2. • What is a barcode?
• Our inspiration for the project
• A summary of the project
• How this project was applied to the “real world”
• Our Project:
‾ Correctly identify unknown or known samples
‾ Identify unknown trees around SUNY New Paltz campus
‾ Identify an unknown insect provided by a Professor
‾ Compare locally sold sushi species to the species label on
their menu
‾ Conclusion
Overview of the Presentation
7. “They found that one-fourth of the fish
samples with identifiable DNA were
mislabeled. A piece of sushi sold as the
luxury treat white tuna turned out to be
Mozambique tilapia, a much cheaper
fish that is often raised by farming. Roe
supposedly from flying fish was
actually from smelt. Seven of nine
samples that were called red snapper
were mislabeled, and they turned out to
be anything from Atlantic cod to
Acadian redfish, an endangered
species.” – The New York Times
Fish Tale Has DNA Hook: Students Find
Bad Labels
12. DNA Copying
• We use primers to copy a region of DNA
• There are five different primers sets , one for each type of organism
• Plant, fungi, invertebrate, vertebrate and fish
Plant Fungi
Animal
15. Gel Electrophoresis
A method that is used for separating and analyzing DNA and their
fragments, based on their size
• Two ladders, of known lengths in the
unit of base pairs, were added to the first
two wells
• When ran, the short DNA strands will be
at the bottom, and longer strands will be
at the top
• Negative charge is at the top while
positive charge is at the bottom
20. • Successful PCR was sent to Genewiz lab, in New Jersey
• Sequences received from Genewiz were then brought to
the Bold Systems website
• Database of organism's DNA sequences
• Compared sequences to databases in three categories
Compare sequence to data
base
35. IN CONCLUSION…
✓ The technique can accurately identify known
and unknown samples
✓ We are confident that the procedure can be
used by other students in a lab setting
36. Works Cited
• "BOLD Systems V3." BOLD Systems V3. BOLD Systems, 2014. Web. 02 Mar. 2016.
• Cenicol, Tony. Tuna Sushi. 2008. The New York Times, New York City. The New York
Times. Web. 15 June 2015.
• "GENEWIZ." GENEWIZ. GENEWIZ, 2016. Web. 02 Mar. 2016.
• Silverman, David. Quotes of the Day. 2008. New York Times, New York City.New York
Times. Web. 15 June 2015.
• Strydhorst, Natasha. 2011. Chimes Calvin College. Chimes Calvin College. Web. 2 Jan.
2016.
• Turner-Juarez, Gwen. Baby Turtle Eating Strawberry. N.d. Wanderings. Web. 15 June
2015.
• Winning, Ethan. Lark Sparrow. 2014. Buntings and New World Sparrows, Walnut Creek,
Contra Costa County, California. Http://ibc.lynxeds.com/. Web. 05 June 2015.
