BIOTECH LECTURES

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Recombinant DNA Technology
Efren E. Magulama
Department of Plant Breeding and Genetics
University of Southern Mindanao
ABE 2017

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rDNA Technology
As a number of experimental
protocols that lead to the transfer
of genetic information (DNA) from
one organism to another

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Basic Techniques in rDNA
technology
Manipulation of DNA in vitro
Transfer of the construct into a host cell
(transformation ) and maintenance of the
cloned DNA in the cell
Cloning or production of many identical
progeny, of phages or plasmids
Identification and selection of the
transformed host cells (those which have
taken up the constructs)
Manipulation of the DNA construct to ensure
production of the protein encoded by the
cloned sequence

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ABE 2017

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ABE 2017

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ABE 2017

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ABE 2017

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ABE 2017

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How to Produce Transgenic Crops
ABE 2017

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Transgenic crops
ABE 2017

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Golden Rice
ABE 2017

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Gene Gun Method
ABE 2017

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ABE 2017

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ABE 2017

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ABE 2017

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ABE 2017

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ABE 2017