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You are interested in using the plasmid pGEE as a vector for incorporating foreign DNA into E.
coli where it can be cloned. The following plasmid map shows the locations of the recognition
sequence (cut) sites of five different restriction endonucleases, as well as the ORI and a
kanamycin-resistance gene (kan'). Pick which pair of restriction endonucleases you would use to
digest the plasmid and the foreign DNA. Note: two combinations are equally good. Find them
both.
Solution
BgII and again using BgII would be a suitable choice because two BgII sites are one after the
other and that would cut a considrable amount of vector DNA, and allow foreign DNA to be
inserted. Making two incisions are necessary for foreign DNA to br inserted.

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You are interested in using the plasmid pGEE as a vector for incorpor.pdf

  • 1. You are interested in using the plasmid pGEE as a vector for incorporating foreign DNA into E. coli where it can be cloned. The following plasmid map shows the locations of the recognition sequence (cut) sites of five different restriction endonucleases, as well as the ORI and a kanamycin-resistance gene (kan'). Pick which pair of restriction endonucleases you would use to digest the plasmid and the foreign DNA. Note: two combinations are equally good. Find them both. Solution BgII and again using BgII would be a suitable choice because two BgII sites are one after the other and that would cut a considrable amount of vector DNA, and allow foreign DNA to be inserted. Making two incisions are necessary for foreign DNA to br inserted.