Principles of operation of coagulometer

1. PRINCIPLES OF
OPERATION OF THE
AUTOMATED
COAGULATION ANALYZER
GROUP THREE

2. INTRODUCTION
• Clotting of blood is one of the most important properties of blood.
• When an individual is hurt, a cascade of reaction (clotting) kicks in to stamp blood
flow by converting it into a gel.
• In the case of excessive or insufficient clotting, it can be extremely harmful.
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3. Continue…
• Coagulation test is performed to analyze relevant anomalies and enable
quick treatment of the same.
• The automated coagulation analyzer is what is used by most
laboratories for this test
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4. AUTOMATED COAGULATION ANALYZER
• Also known as coagulometer, is an analyzer used to
test the coagulation efficiency of blood so as to
diagnose and assess bleeding disorders such as
hemophilia or to monitor patients who are taking
anticoagulant medicines such as aspirin, heparin, or
warfarin.
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5. Continue…
• It generates reliable data in a fast and simple manner.
• Despite it’s precision, the traditional tilt tube method remains
the golden standard for clot-based coagulation studies
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6. PRINCIPLES OF OPERATION OF THE
COAGULOMETER
• The various coagulometers available have different means of detecting the end-
point, which may make comparison of results difficult. Some commonly used
techniques follow:
• Electromechanical;
Impedance, steel ball, rotating cuvette
Impedance, steel ball, rotating steel ball
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7. Continue…
• Photo-optical analysis;
Scattered light detection for clotting assays
Transmitted light detection for chromogenic assays
Transmitted light detection for immunoassays
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8. Impedance, steel ball, rotating cuvette
• The sample cuvette rotates and a steel ball
remains stationary in a magnetic field until the
formation of fibrin strands around the ball
produces movement.
• This is detected by a change in the magnetic field
and the coagulation time is recorded. 10/04/2023 2:20 carra 8

9. Impedance, steel ball, rotating steel ball
• A steel ball rotates under the influence of a magnet until the formation of
fibrin strands around the ball stops it rotating.
• This is detected by a sensor and the coagulation time is recorded.
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10. Scattered light detection for clotting assays
• The increasing turbidity during the formation of a fibrin clot is measured as an
increase in scattered light intensity when exposed to light at a wavelength of
660 nm.
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11. Transmitted light detection for chromogenic
assays (405 nm, 575 nm, 800 nm)
• Colour production leads to a change in light absorbance, which is detected as a
change in transmitted light. The change in absorbance per minute is calculated (Δ
optical density (OD)/min). Various wavelengths can be used, such as 405 nm,
575 nm and 800 nm.
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12. Transmitted light detection for immunoassays
(405 nm, 575 nm, 800 nm)
• The change in light absorbance caused by the antigen–antibody reaction is
detected as the change in transmitted light. The change in absorbance per
minute is calculated (Δ OD/min). Some analyzers detect light transmittance
at multiple wavelengths between 395 and 710 nm.
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13. THANK YOU
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14. MEMBERS AND THEIR INDEX NUMBERS
NAME INDEX NUMBER
Adoku Emmanuel 052142060037
Isaac Sam 052142060093
Hussein Yusha 052142060075
Osei Sarfoah Priscilla 052142060083
Mustapha Maryam 052142060081
Essel Philip 052142060013
Kanyun Wisdom 052142060078
Agyei Badu Samuel 052142060048
Fati Iddriss Adams 052142060046
Oppong Richmond 052142060106
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