traitement des eaux usées par phytoremédiation en zone aride, phytoremédiation des hydrocarbures aromatiques polycycliques, réponse au stress des hydrocarbures aromatiques polycycliques pour les modèles de plantes glycophytes et les halophytes

1. Rencontres de l’ingénierie écologique en Bretagne
Pôle Phytoremédiation
Titre Contact Société / Organisme N°0
Mohamed Laboratoire ECOBIO
TREATMENT OF WASTEWATER BY PHYTOREMEDIATION IN ARID AREA SAGGAÏ 28
Mounir (CNRS, UR1, OSUR)
Laboratoire ECOBIO
PHYTOREMEDIATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) Anne-Sophie DUMAS 29
(CNRS, UR1, OSUR)
STRESS RESPONSE TO POLYCYCLIC AROMATIC HYDROCARBON OF Laboratoire ECOBIO
MOEZ SHIRI 30
GLYCOPHYTES AND HALOPHYTES PLANT MODELS (CNRS, UR1, OSUR)
23 janvier 2013 – Carrefour des Gestions Locales de l’Eau

2. Treatment of wastewater by phytoremediation in arid area
Mohamed Mounir SAGGAÏ1, Moez SHIRI2, Anne-Sophie DUMAS2, Ryad DJERIBI3 and Abdelhak EL AMRANI 2
1 Ouargla University (Algeria)
2 Rennes1 University (France)/ ECOBIO Laboratory
3 Annaba University (Algeria) 28
Macrophytes Phyto-purification Domestic wastewater Organic load Arid climate
Pic.1
Captions
 Research topic Pic.1 Schematic design of the processing station
by macrophytes
- Ecotoxicology Pic. 2 Input-output balance for BOD5 (Biological
Demand of Oxygen )
- Ecological restoration Pic. 3 Input-output balance for COD (Chemical
Demand of Oxygen )
- Phytoremediation Pic. 4 Variation of phenanthrene (enter, center
and Exit)
 Activities and Expertise
Design, construction and control of Wet Areas
Wastewater Treatment, Pic. 2 Pic. 3 Pic. 4
Image 3
Abstract
The aim of this study was the monitoring of the effectiveness of a system of sewage from domestic waste through the use of macrophytes in
an arid climate. The experimental system was designed as fellow; the duration of treatment was 07 days (Pic.1). The reactor effluent through
the basin by gravity with a flow of 4 L/min. The retention time in the decanter was 60 hours. Irrigation frequency is weekly. The parameters
studied are: (TSS, BOD5, COD, EC, pH), nutrients (total nitrogen: total phosphorus and TKN: TP) and microbiological parameters.
The results showed encouraging performance, decline in organic load and microbiologic load (TSS: 95.75%, BOD5: 91.15%, COD: 91.92%,
PT: 24.90% pH 7.5).
Thematic topic
The arid climate and vegetation gives specificity to Saharan regions, which Our Analyzes allowed us to determine the potential of WWG system.
are characterized by a typical ecological fragility, this requires caution in A new approach was developed with the laboratory of ECOBIO for the
approaches and choices to deal with environmental problems. treatment of PAHs.
phytoremediation approach may be used to clean up several sources of The species studied showed high capability to reduce the concentration
pollution, the challenge of this project was to exploit the flora of these regions and of PAHs (Pic. 4)
their potential to serve this choice (Pic.1). These preliminary results open opportunities for broader uses of
 Problematic Saharan plants for phytoremediation.
The project was led and determined to control the potential of a water The project partners are:
treatment system by macrophytes (plants that grown in the study area), and study
the behavior of Selected species without forgetting the quality of the water obtained - Ministry of Water Resources (Algeria)
after treatment (PIC. 2, 3 & 4) . - National Sanitation Office (ONA)
 Subjects of study - Wastewater Garden® (WWG).
• Selected plant can reduce pollution levels to acceptable levels? - APC Témacine
• The cleaning up capability is more or less stable throughout the year? - University of Rennes 1
Coordonnées
CNRS UMR 6553 Mohamed Mounir SAGGAÏ/ Abdelhak El Amrani
6553 Ecobio: Ecosystème,Biodiversité, Evolution abdelhak.elamrani@univ-rennes1.fr
Campus de Beaulieu, CS 742005 mohamed-mounir.saggai@univ-rennes1.fr
35042 Rennes Cedex, France
Les Rencontres de l’ingénierie écologique en Bretagne, au 14e Carrefour des Gestions Locales de l’Eau, le 23 Janvier 2013 à Rennes

3. Phytoremediation of polycyclic aromatic hydrocarbons (PAHs)
Anne-Sophie Dumas1, Richard Berthomé2, Moez Shiri1, Mohamed Mounir Saggaï1 and Abdelhak El Amrani1
1CNRS,Université de Rennes 1, UMR 6553, Ecosystèmes-Biodiversité-Evolution, 35042 Rennes cedex, France,
2URGV, UMR INRA 1165 - CNRS 8114 - UEVE, 2, Rue Gaston Cremieux, CP5708, 91057 Evry cedex, France.
29
Phytoremediation PAH Field trial Molecular mechanisms Plants Green technology
 Fields of Research  Activities
o Decontamination of PAH-contaminated sites o Design and monitoring of PAH phytoremediation sites
o Green/ sustainable technologies o Molecular biology, in-vitro culture, GC-MS analysis
o Interface environmental stress-genome o Transcriptomics , metabolomics
Projects:
• In-vitro analysis of PAHs effects on model plant, Arabidopsis thaliana,
to understand how plants cope with PAHs stress at different scales
(Fig 3.) :
 Plant development in PAH contaminated media
 PAH effects on genome expression (1) and on metabolome at short-term (30
min to 24h)
 PAH quantification in plants (2) and localization of PAH in plants.
 Some additives, such as sucrose, enhance plant tolerance to some
xenobiotics: is it appliable to PAHs?
• Design and monitoring of a full-scale field trial of PAH
phytoremediation: comparison between several plant species, impact
of bringing a uncontaminated soil with its micro-organisms and worms
on the rates of PAH degradation (3)
 Issues
o How to optimize in-situ phytoremediation of PAH?
o What are the processes of detoxification of PAHs in plants?
 Studies Possible projects for the future…
o Create remediation systems combining plants, micro-organisms and
agricultural practices (amendments, tillage) that are adapted to the site, • Testing other species and a mix of several
species (trees and grass) on a PAH
the type of contaminant
contaminated soil
o Identify and optimize the mechanisms of plants PAH metabolism
• Create a guideline that will help in the set-up
o Use our knowledge on detoxification systems in plants and micro- of a phytoremediation system
organisms to enhance phytoremediation by creating or selecting plants Determination of the adapted species
species that are more tolerant and more efficient Establishing a timeline of treatment
Collaborations
(1) URGV, INRA-CNRS, Evry, France
(2) Géosciences, CNRS - University of Rennes 1, Rennes, France
(3) Axson (formerly Bs Coatings), Aubevoye, France.
UMR 6553 Ecobio- Université de Rennes 1- CNRS Contact
Campus de Beaulieu, Avenue du Général Leclerc Anne-Sophie Dumas (PhD student) - Abdelhak El Amrani (Supervisor)
35042 RENNES Cedex – France +33 (0)2 23 23 58 26
http://ecobio.univ-rennes1.fr/news.php anne-sophie.dumas@univ-rennes1.fr
Les Rencontres de l’ingénierie écologique en Bretagne
14e Carrefour des Gestions Locales de l’Eau,
23 Janvier 2013 - Rennes

4. Stress response to polycyclic aromatic hydrocarbon of glycophytes and
Halophytes plant models (Arabidopsis thaliana, Thellungiella salsuginea)
1CNRS,
Moez Shiri1, Med Mounir Saggaï, Anne-Sophie Dumas, Chedly Abdelly1 and Abdelhak El Amrani1
Université de Rennes 1, UMR 6553, Ecosystèmes-Biodiversité-Evolution, équipe Mécanismes à l’Origine, LPE, laboratoire des plantes extrêmophiles
30
de la Biodiversité, 35042 Rennes cedex, France
Polycyclic aromatic
phytoremediation Oxidative stress Abiotic stress Glycophyte Halophyte
hydrocarbon
Knowledge - skills Response of plants to PAH stress
studies of the mechanism of tolerance to abiotic stress.
 Research project
o hydrocarbon pollution
o functional genomics of phytoremediation
Image 1
o Bioremediation and extremophile plants
o Activities and Expertise
o treatment of contaminated sites by organic pollution and
heavy metals
o Application of biological processes…
Image 4
Project
This thesis project is a collaboration between Rennes university and the Laboratory of
Biotechnology and Extremophiles Plant of Borj Cedria center. It will study the physiological,
biochemical and molecular responses of plants to abiotic stress.
This study will focus on how plants may clean up contaminated environment.
 Hypothesis
Extremophile plants have significant potential in terms of
adaptation and tolerance. They have already proven their
ability to grow in extreme conditions, and were used to
desalinate and decontaminate soil polluted by heavy metal.
These traits may help extremophile plants to clean up PAH-
contaminated sites.
 Issues
o Identify the mechanisms of degradation and sequestration of
PAHs in extremophile plants.
o Identify genes involved in stress tolerance towards PAHs
o Isolate the gene or genes responsible for PAHs degradation and
overexpress them in plant in order to increase the remedial
competitiveness
UMR 6553 Ecobio Moez Shiri/ Abdelhak El Amrani
Avenue du Général Leclerc Campus Beaulieu, bât 14a, p.122
Campus de Beaulieu CS 74205 - 35042 RENNES CEDEX
35042 RENNES Cedex - France 02.23.23.58.26
33.(0)2.23.23.58.26
moez.shiri@univ-rennes1.f.fr
http://ecobio.univ-rennes1.fr
abdelhak.elamrani@univ-rennes1.fr
Les Rencontres de l’ingénierie écologique en Bretagne, au 14e Carrefour des Gestions Locales de l’Eau, le 23 Janvier 2013 à Rennes