Counting immunoassay (CIA)
•
0 likes•70 views
In CIA, polystyrene beads are coated with a number of antibodies that are complementary to the target antigens. During incubation, the beads bind to a variety of antigens and jointly form a large mass, but some beads are not bound. The whole solution passes through a cell counter, with only unbound beads counted. The amount of unbound beads is inversely proportional to that of antigens.
Report
Share
Report
Share
Download to read offline
Recommended
Medical abbreviations i, march 24, 13
This document contains a list of common medical abbreviations and their meanings. It includes abbreviations such as # for fracture, 1/12 for 1 month, and 1/52 for 1 week. The list continues for several pages covering abbreviations like A&E for accident and emergency, a&w for alive and well, and a.c. for before meals. The document is authored by Dr. Juan Carlos Díaz Torre, a pediatric neonatologist, and provides contact information.
Contents and front matter
This book provides a practical guide to preimplantation genetic diagnosis (PGD). It discusses the various approaches to obtaining biopsy material from embryos and gametes for single-cell genetic analysis. It covers PGD for single-gene disorders, chromosomal disorders, HLA typing, and the establishment of human embryonic stem cell lines. The book also addresses ethical, social, and legal considerations of PGD.
Apoptosis manual roche
This document is the table of contents for the 3rd edition of the book "Apoptosis, Cell Death, and Cell Proliferation" published by Roche Applied Science. The table of contents outlines the chapters and sections of the book, including an introduction to terminology of cell death and differences between necrosis and apoptosis. It also lists methods for studying apoptosis and cytotoxicity in cell populations and individual cells, such as assays measuring DNA fragmentation, proteases, membrane alterations, and metabolic activity. Product names and manufacturers are provided for the different assay methods.
Case Study Task: Diagnose the Patient
For the IB Biology course. Based on the Case of the Crooked Cell. Credits at the end of the document to avoid giving away the case.
Wiley et al PRER
This document discusses the use of patient-specific induced pluripotent stem cells (iPSCs) for modeling and developing treatments for inherited retinal degenerative diseases. It describes how iPSCs can be used to model various retinal diseases through disease-specific iPSC lines. Additionally, it explores how iPSCs may be applied to test gene therapies and drug candidates, aid genome editing efforts, and serve as a source of cells for transplantation treatments. Overall, the document outlines the potential of iPSC technology to further our understanding of retinal diseases and develop novel therapeutic strategies.
Waters protein therapeutics application proctocols
This document discusses various techniques for characterizing biopharmaceuticals such as proteins and antibodies using liquid chromatography coupled with mass spectrometry (LC/MS). It provides several examples of using LC/MS to characterize monoclonal antibodies and their components such as intact antibodies, substructures, variable regions, and glycan profiles. It also discusses techniques for characterizing peptides generated from protein digestion, including peptide mapping and glycopeptide analysis. The document aims to demonstrate solutions for analyzing biopharmaceuticals using LC/MS.
Passarge toc
This document appears to be the table of contents for a genetics textbook. It lists over 200 topics related to genetics and genomics, organized under headings such as "Fundamentals", "Analysis of DNA", "Eukaryotic Cells", "Formal Genetics", "Genomics", and "Genetics and Medicine". Some of the topics listed include DNA structure, DNA replication, transcription and translation, genetic code, restriction enzymes, DNA sequencing, cell division, Mendelian inheritance, linkage and recombination, chromosomes, gene regulation, epigenetics, signal pathways, embryogenesis, genome analysis, disease-causing genes, cancer genetics, and inherited disorders.
Cellular immunity and immunopathology in autoimmune addison’s disease
The document summarizes current knowledge about cellular immunity and immunopathology in autoimmune Addison's disease (AAD). It discusses that AAD results from selective destruction of steroid hormone-secreting cells in the adrenal cortex by immune-mediated inflammation (adrenalitis). While T cells are thought to play a major role in obliterating adrenocortical cells, the mechanisms behind how autoimmune adrenalitis is initiated and perpetuated remain enigmatic. The review provides an overview of the histopathology seen in AAD and the roles of genetic predisposition, endogenous factors, autoantibodies, and cellular immunity in the pathogenesis of the disease. It proposes a hypothesis for how the autoimmune process
Recommended
Medical abbreviations i, march 24, 13
This document contains a list of common medical abbreviations and their meanings. It includes abbreviations such as # for fracture, 1/12 for 1 month, and 1/52 for 1 week. The list continues for several pages covering abbreviations like A&E for accident and emergency, a&w for alive and well, and a.c. for before meals. The document is authored by Dr. Juan Carlos Díaz Torre, a pediatric neonatologist, and provides contact information.
Contents and front matter
This book provides a practical guide to preimplantation genetic diagnosis (PGD). It discusses the various approaches to obtaining biopsy material from embryos and gametes for single-cell genetic analysis. It covers PGD for single-gene disorders, chromosomal disorders, HLA typing, and the establishment of human embryonic stem cell lines. The book also addresses ethical, social, and legal considerations of PGD.
Apoptosis manual roche
This document is the table of contents for the 3rd edition of the book "Apoptosis, Cell Death, and Cell Proliferation" published by Roche Applied Science. The table of contents outlines the chapters and sections of the book, including an introduction to terminology of cell death and differences between necrosis and apoptosis. It also lists methods for studying apoptosis and cytotoxicity in cell populations and individual cells, such as assays measuring DNA fragmentation, proteases, membrane alterations, and metabolic activity. Product names and manufacturers are provided for the different assay methods.
Case Study Task: Diagnose the Patient
For the IB Biology course. Based on the Case of the Crooked Cell. Credits at the end of the document to avoid giving away the case.
Wiley et al PRER
This document discusses the use of patient-specific induced pluripotent stem cells (iPSCs) for modeling and developing treatments for inherited retinal degenerative diseases. It describes how iPSCs can be used to model various retinal diseases through disease-specific iPSC lines. Additionally, it explores how iPSCs may be applied to test gene therapies and drug candidates, aid genome editing efforts, and serve as a source of cells for transplantation treatments. Overall, the document outlines the potential of iPSC technology to further our understanding of retinal diseases and develop novel therapeutic strategies.
Waters protein therapeutics application proctocols
This document discusses various techniques for characterizing biopharmaceuticals such as proteins and antibodies using liquid chromatography coupled with mass spectrometry (LC/MS). It provides several examples of using LC/MS to characterize monoclonal antibodies and their components such as intact antibodies, substructures, variable regions, and glycan profiles. It also discusses techniques for characterizing peptides generated from protein digestion, including peptide mapping and glycopeptide analysis. The document aims to demonstrate solutions for analyzing biopharmaceuticals using LC/MS.
Passarge toc
This document appears to be the table of contents for a genetics textbook. It lists over 200 topics related to genetics and genomics, organized under headings such as "Fundamentals", "Analysis of DNA", "Eukaryotic Cells", "Formal Genetics", "Genomics", and "Genetics and Medicine". Some of the topics listed include DNA structure, DNA replication, transcription and translation, genetic code, restriction enzymes, DNA sequencing, cell division, Mendelian inheritance, linkage and recombination, chromosomes, gene regulation, epigenetics, signal pathways, embryogenesis, genome analysis, disease-causing genes, cancer genetics, and inherited disorders.
Cellular immunity and immunopathology in autoimmune addison’s disease
The document summarizes current knowledge about cellular immunity and immunopathology in autoimmune Addison's disease (AAD). It discusses that AAD results from selective destruction of steroid hormone-secreting cells in the adrenal cortex by immune-mediated inflammation (adrenalitis). While T cells are thought to play a major role in obliterating adrenocortical cells, the mechanisms behind how autoimmune adrenalitis is initiated and perpetuated remain enigmatic. The review provides an overview of the histopathology seen in AAD and the roles of genetic predisposition, endogenous factors, autoantibodies, and cellular immunity in the pathogenesis of the disease. It proposes a hypothesis for how the autoimmune process
Pubid783354509
This document provides a table of contents for a textbook on clinical immunology. The table of contents lists over 50 chapters organized into sections on fundamental immunological principles, laboratory applications, clinical immunology, tumor immunology, transplantation, musculoskeletal diseases, autoantibodies, connective tissue diseases and vasculitis, and many other areas relating to the immune system and diseases involving immune mechanisms. The chapters cover topics such as immune cell development and function, antigen presentation, complement activation, immunodeficiencies, HIV infection, autoimmune diseases of various organ systems, and clinical uses of immunotherapies and immunosuppressive drugs.
Advances and technical standards in neurosurgery
This document is the contents page for a book titled "Advances and Technical Standards in Neurosurgery Vol. 34". It lists contributors and chapter titles for articles on various topics in neurosurgery, including adjuvant treatment for high-grade astrocytic glioma, deep brain stimulation for psychiatric disorders, and the surgical technique for high flow extracranial to intracranial vascular bypass for giant aneurysms. The contents page provides an overview of the chapters and topics covered in the neurosurgery book.
Kl ph d_thesisfinal
This PhD thesis presents a mathematical model of the signalling pathways regulating endothelial cell responses to fluid shear stress. The model consists of 8 interconnected modules describing processes such as calcium dynamics, calpain activity, integrin activation, and the phosphorylation of proteins like FAK and Src. Differential equations are used to simulate the dynamic behaviour of molecular species over time under varying shear stress conditions. The model aims to improve understanding of how fluid flow stimulation is converted into biochemical signals in endothelial cells and how this relates to the development of atherosclerosis.
At
This thesis describes the development of a multiplex polymerase chain reaction (PCR) assay to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. The PCR uses primers specific to conserved capsular genes to detect all A. pleuropneumoniae strains except serotype 4, and a separate set of primers specific to serotype 5 capsular genes. The sensitivity of the assay was determined to be less than 102 colony forming units. This PCR assay enables definitive distinction of serotype 5 strains from other serotypes without potential cross-reactivity issues of antibody-based methods.
Who blood products
This document provides recommendations for the production, control, and regulation of human plasma for fractionation. It covers appropriate donor selection and screening, production methods, collection, separation, freezing and storage of plasma. The goal is to exclude infectious donations and ensure plasma is produced according to good manufacturing practices. Precautions are outlined to prevent transmission of pathogens from blood and plasma through proper donor screening, epidemiological surveillance, adherence to procedures, and testing and quality control of plasma units.
Inglis PhD Thesis
This dissertation examines methods for measuring the spatial arrangement of neurons and glial cells in the mammalian cortex. The document begins with an introduction discussing the importance of studying brain cell arrangement and the need for quantitative tools. It then provides a literature review on brain anatomy, spatial arrangement mechanisms, and existing measurement theories. The experimental method section describes a three-part process: 1) digitizing tissue samples at high resolution, 2) developing algorithms to recognize cells in the digitized images, and 3) analyzing the data using metrics like cell counts, density maps, and cross-correlations. Results are presented on tissue samples from the macaque monkey and rat brain, focusing on specific cortical areas. Future studies are proposed to integrate the data, analyze
Revision questions on 1.6. Cell division of IB Biology
This document contains revision questions about cell division, including mitosis and cytokinesis. It begins by defining key terms like mitosis, interphase, and the phases of the cell cycle. It then provides questions to test understanding of topics like DNA supercoiling during mitosis, the stages of mitosis, and calculating mitotic indices from micrographs. Later questions address the differences between animal and plant cytokinesis and the role of cyclins in cell cycle control. It concludes by defining cancer-related topics and analyzing a graph showing the correlation between cigarette smoking and lung cancer deaths.
(SpringerBriefs in Public Health) Duncan Dartrey Adams, Christopher Dartrey A...
Autoimmune Disease_ Pathogenesis, Genetics, Immunotherapy, Prophylaxis and Principles for Organ Transplantation
A2 OCR Biology - Unit 1 Module 1 Revision Test
This document appears to be a quiz on biology concepts related to communication and homeostasis. It contains 34 multiple choice questions testing understanding of topics like homeostasis, temperature regulation, sensory receptors, action potentials, synapses, glands, hormones, and the cardiovascular center of the brain. The questions require defining key terms, naming examples, and describing physiological mechanisms and relationships between different biological systems and components.
Pathophysiology a practical approach (Lachel Story) (z-lib.org).pdf
This document provides information about the third edition of the textbook "Pathophysiology: A Practical Approach" by Lachel Story. It was published in 2018 by Jones & Bartlett Learning and discusses pathophysiology from a nursing perspective. The book contains 7 chapters that cover cellular function, immunity, hematopoietic function, cardiovascular function, respiratory function, fluid/electrolyte/acid-base homeostasis, and urinary function. It is intended to help nurses understand disease processes and apply pathophysiological principles to patient care.
Sma12 4668: Clinical Drug Testing in Primary Care
This document provides guidance on clinical drug testing in primary care. It discusses the reasons drug testing can be useful in primary care settings, including monitoring prescription medication use, evaluating unexplained symptoms, and detecting substance use disorders. The document covers important terminology, different testing methods and matrices, considerations for selecting laboratory or point-of-care testing, how to prepare the clinical setting and staff, and how to discuss testing with patients while being culturally sensitive. The goal is to help primary care providers appropriately incorporate and utilize drug testing in their practices.
Absolute Obstetric Anesthesia Review.pdf
This document provides information about a study guide book for obstetric anesthesia certification and recertification. The book is titled "Absolute Obstetric Anesthesia Review" and is authored by Cassandra Wasson, Albert Kelly, David Ninan, and Quy Tran. It is intended to provide a concise study guide for anesthesiology residents, obstetric anesthesiology fellows, and anesthesiologists providing care to obstetric patients. The book is organized based on the outline from the American Board of Anesthesiology and covers topics relevant to the boards and clinical practice of obstetric anesthesia.
PhD Thesis Book
This thesis aimed to identify and validate novel biomarkers for prostate cancer (PCa) diagnosis and prognosis. The mRNA expression levels of 14 genes were measured in prostate tissue samples from men with and without PCa using quantitative reverse transcription PCR. The results showed statistically significant overexpression of 13 genes in PCa tissues compared to non-cancerous tissues. AMACR mRNA was upregulated even in histologically benign areas, indicating potential "field effects". Significant differences were observed between low and high Gleason grades, relapse and non-relapse, and low and high TNM stages. Knockdown experiments in PCa cell lines showed effects on cell invasion and morphology for several genes. Expression of TDRD1 and TMPRSS2-
[] Molecular cancer_therapeutics_strategies_for_d(book_zz.org)
This document is the table of contents for a book titled "Molecular Cancer Therapeutics: Strategies for Drug Discovery and Development". It lists the titles and authors of each chapter in the book. The book contains 8 chapters that cover topics such as cancer genetics, RNA interference, tissue arrays, protein transduction strategies, drug screening assay development, and gene microarray technologies for cancer drug discovery. The table of contents provides a high-level overview of the topics and contributors included in this book on molecular cancer therapeutics.
AS OCR Biology - Unit 1 - Module 1 Revision Test
This document contains a 59 question quiz on cells and cell biology. It covers topics like cell structure, organelles, transport mechanisms, cell division and tissues. For each question, space is provided to write the answer. The quiz is designed to test understanding of key topics in the AS OCR Biology module on cells. It will be marked out of 73 and the percentage score calculated. A QR code is also provided to link to additional online resources for revision.
TaqMan® Gene Expression Assays Protocol
This TaqMan® Gene Expression Assays Protocol provides instructions for performing
real-time reverse transcription-PCR (real-time RT-PCR) using TaqMan Gene
Expression Assays and TaqMan Non-coding RNA Assays.
For more information visit:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/PCR/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/single-tube-taqman-gene-expression-analysis.html?ICID=search-product?CID=TaqManGeneProducts-SS-12312
Shere Billouin-Frazier_Drexel Thesis_May 2010
This document discusses Mycosis Fungoides (MF) and Sezary Syndrome (SS), which are subtypes of Cutaneous T-cell Lymphoma (CTCL). MF and SS are relatively rare but increasing in incidence. The etiology of MF and SS is still unknown, though environmental exposures, genetic predisposition, and infectious agents have been investigated as potential causes. The document provides background on the epidemiology, clinical manifestations, staging, and pathophysiology of MF and SS. It also discusses current treatments for CTCL including histone deacetylase inhibitors.
Atlas of salivary gland pathology
This document is an atlas describing the pathology of salivary gland neoplasms. It begins with chapters on the gross and microscopic anatomy of salivary glands. Subsequent chapters provide detailed descriptions and photomicrographs of various benign and malignant salivary gland tumors, organized by histologic type. The goal of the atlas is to aid in the diagnosis of salivary gland neoplasms for pathologists and head and neck specialists.
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...National Institute for Cellular Biotechnology
First ESACT Frontiers Retreat, 22-26th October, 2016 (Lyon, France) Program & Abstract Book
The European Society for Animal Cell Technology (ESACT) brings together scientists, engineers and other specialists working with animal cells in order to promote communication of experiences between European and International investigators and progress the development of cell systems and their derived products. ESACT was founded in 1976 to create a forum for the exchange of ideas on biological and engineering techniques to promote knowledge and the use of human and animal cells e.g. for the manufacturing of products. Members include scientists and engineers in academic, medical and industrial R&D and Production at applied science institutions and universities, in the medical services, in industry, and in the political and regulatory bodies. ESACT actively encourages interactions between academia, governmental and policy making agencies and the manufacturing and service industries.
Integrin Signalling And Cancer Front Pgs
This thesis submitted by Erik Freiter examines the role of integrin signaling and Src tyrosine kinase in cytoskeletal rearrangement during melanoma metastasis. Freiter hypothesized that Src activity reduces RhoA-mediated cytoskeletal rearrangement. Using cultured metastatic melanoma cells and primary melanocytes, Freiter altered Src activity exogenously. He observed an increase in GTP-bound RhoA when Src activity was decreased but no change in GTP-bound Rac1. The data suggest RhoA is involved in cytoskeletal remodeling downstream of Src in melanoma cells. Src may activate the regulator p190RhoGAP, reducing GTP-bound RhoA levels. Rac1 did not appear to be activated by Src in this model.
ADC conjugate sites analysis
Antibody-drug conjugates employ the specific monoclonal antibodies (mAbs) to achieve targeted delivery of the conjugated cytotoxic molecules to tumor cells.
https://www.creative-biolabs.com/adc/conjugate-sites-analysis.htm
adc chemistry
As one major component of an antibody-drug conjugate (ADC), the antibody is the key for target specificity and serves as the cargo to deliver the cytotoxic drug (payload).
https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm
More Related Content
Similar to Counting immunoassay (CIA)
Pubid783354509
This document provides a table of contents for a textbook on clinical immunology. The table of contents lists over 50 chapters organized into sections on fundamental immunological principles, laboratory applications, clinical immunology, tumor immunology, transplantation, musculoskeletal diseases, autoantibodies, connective tissue diseases and vasculitis, and many other areas relating to the immune system and diseases involving immune mechanisms. The chapters cover topics such as immune cell development and function, antigen presentation, complement activation, immunodeficiencies, HIV infection, autoimmune diseases of various organ systems, and clinical uses of immunotherapies and immunosuppressive drugs.
Advances and technical standards in neurosurgery
This document is the contents page for a book titled "Advances and Technical Standards in Neurosurgery Vol. 34". It lists contributors and chapter titles for articles on various topics in neurosurgery, including adjuvant treatment for high-grade astrocytic glioma, deep brain stimulation for psychiatric disorders, and the surgical technique for high flow extracranial to intracranial vascular bypass for giant aneurysms. The contents page provides an overview of the chapters and topics covered in the neurosurgery book.
Kl ph d_thesisfinal
This PhD thesis presents a mathematical model of the signalling pathways regulating endothelial cell responses to fluid shear stress. The model consists of 8 interconnected modules describing processes such as calcium dynamics, calpain activity, integrin activation, and the phosphorylation of proteins like FAK and Src. Differential equations are used to simulate the dynamic behaviour of molecular species over time under varying shear stress conditions. The model aims to improve understanding of how fluid flow stimulation is converted into biochemical signals in endothelial cells and how this relates to the development of atherosclerosis.
At
This thesis describes the development of a multiplex polymerase chain reaction (PCR) assay to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. The PCR uses primers specific to conserved capsular genes to detect all A. pleuropneumoniae strains except serotype 4, and a separate set of primers specific to serotype 5 capsular genes. The sensitivity of the assay was determined to be less than 102 colony forming units. This PCR assay enables definitive distinction of serotype 5 strains from other serotypes without potential cross-reactivity issues of antibody-based methods.
Who blood products
This document provides recommendations for the production, control, and regulation of human plasma for fractionation. It covers appropriate donor selection and screening, production methods, collection, separation, freezing and storage of plasma. The goal is to exclude infectious donations and ensure plasma is produced according to good manufacturing practices. Precautions are outlined to prevent transmission of pathogens from blood and plasma through proper donor screening, epidemiological surveillance, adherence to procedures, and testing and quality control of plasma units.
Inglis PhD Thesis
This dissertation examines methods for measuring the spatial arrangement of neurons and glial cells in the mammalian cortex. The document begins with an introduction discussing the importance of studying brain cell arrangement and the need for quantitative tools. It then provides a literature review on brain anatomy, spatial arrangement mechanisms, and existing measurement theories. The experimental method section describes a three-part process: 1) digitizing tissue samples at high resolution, 2) developing algorithms to recognize cells in the digitized images, and 3) analyzing the data using metrics like cell counts, density maps, and cross-correlations. Results are presented on tissue samples from the macaque monkey and rat brain, focusing on specific cortical areas. Future studies are proposed to integrate the data, analyze
Revision questions on 1.6. Cell division of IB Biology
This document contains revision questions about cell division, including mitosis and cytokinesis. It begins by defining key terms like mitosis, interphase, and the phases of the cell cycle. It then provides questions to test understanding of topics like DNA supercoiling during mitosis, the stages of mitosis, and calculating mitotic indices from micrographs. Later questions address the differences between animal and plant cytokinesis and the role of cyclins in cell cycle control. It concludes by defining cancer-related topics and analyzing a graph showing the correlation between cigarette smoking and lung cancer deaths.
(SpringerBriefs in Public Health) Duncan Dartrey Adams, Christopher Dartrey A...
Autoimmune Disease_ Pathogenesis, Genetics, Immunotherapy, Prophylaxis and Principles for Organ Transplantation
A2 OCR Biology - Unit 1 Module 1 Revision Test
This document appears to be a quiz on biology concepts related to communication and homeostasis. It contains 34 multiple choice questions testing understanding of topics like homeostasis, temperature regulation, sensory receptors, action potentials, synapses, glands, hormones, and the cardiovascular center of the brain. The questions require defining key terms, naming examples, and describing physiological mechanisms and relationships between different biological systems and components.
Pathophysiology a practical approach (Lachel Story) (z-lib.org).pdf
This document provides information about the third edition of the textbook "Pathophysiology: A Practical Approach" by Lachel Story. It was published in 2018 by Jones & Bartlett Learning and discusses pathophysiology from a nursing perspective. The book contains 7 chapters that cover cellular function, immunity, hematopoietic function, cardiovascular function, respiratory function, fluid/electrolyte/acid-base homeostasis, and urinary function. It is intended to help nurses understand disease processes and apply pathophysiological principles to patient care.
Sma12 4668: Clinical Drug Testing in Primary Care
This document provides guidance on clinical drug testing in primary care. It discusses the reasons drug testing can be useful in primary care settings, including monitoring prescription medication use, evaluating unexplained symptoms, and detecting substance use disorders. The document covers important terminology, different testing methods and matrices, considerations for selecting laboratory or point-of-care testing, how to prepare the clinical setting and staff, and how to discuss testing with patients while being culturally sensitive. The goal is to help primary care providers appropriately incorporate and utilize drug testing in their practices.
Absolute Obstetric Anesthesia Review.pdf
This document provides information about a study guide book for obstetric anesthesia certification and recertification. The book is titled "Absolute Obstetric Anesthesia Review" and is authored by Cassandra Wasson, Albert Kelly, David Ninan, and Quy Tran. It is intended to provide a concise study guide for anesthesiology residents, obstetric anesthesiology fellows, and anesthesiologists providing care to obstetric patients. The book is organized based on the outline from the American Board of Anesthesiology and covers topics relevant to the boards and clinical practice of obstetric anesthesia.
PhD Thesis Book
This thesis aimed to identify and validate novel biomarkers for prostate cancer (PCa) diagnosis and prognosis. The mRNA expression levels of 14 genes were measured in prostate tissue samples from men with and without PCa using quantitative reverse transcription PCR. The results showed statistically significant overexpression of 13 genes in PCa tissues compared to non-cancerous tissues. AMACR mRNA was upregulated even in histologically benign areas, indicating potential "field effects". Significant differences were observed between low and high Gleason grades, relapse and non-relapse, and low and high TNM stages. Knockdown experiments in PCa cell lines showed effects on cell invasion and morphology for several genes. Expression of TDRD1 and TMPRSS2-
[] Molecular cancer_therapeutics_strategies_for_d(book_zz.org)
This document is the table of contents for a book titled "Molecular Cancer Therapeutics: Strategies for Drug Discovery and Development". It lists the titles and authors of each chapter in the book. The book contains 8 chapters that cover topics such as cancer genetics, RNA interference, tissue arrays, protein transduction strategies, drug screening assay development, and gene microarray technologies for cancer drug discovery. The table of contents provides a high-level overview of the topics and contributors included in this book on molecular cancer therapeutics.
AS OCR Biology - Unit 1 - Module 1 Revision Test
This document contains a 59 question quiz on cells and cell biology. It covers topics like cell structure, organelles, transport mechanisms, cell division and tissues. For each question, space is provided to write the answer. The quiz is designed to test understanding of key topics in the AS OCR Biology module on cells. It will be marked out of 73 and the percentage score calculated. A QR code is also provided to link to additional online resources for revision.
TaqMan® Gene Expression Assays Protocol
This TaqMan® Gene Expression Assays Protocol provides instructions for performing
real-time reverse transcription-PCR (real-time RT-PCR) using TaqMan Gene
Expression Assays and TaqMan Non-coding RNA Assays.
For more information visit:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/PCR/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/single-tube-taqman-gene-expression-analysis.html?ICID=search-product?CID=TaqManGeneProducts-SS-12312
Shere Billouin-Frazier_Drexel Thesis_May 2010
This document discusses Mycosis Fungoides (MF) and Sezary Syndrome (SS), which are subtypes of Cutaneous T-cell Lymphoma (CTCL). MF and SS are relatively rare but increasing in incidence. The etiology of MF and SS is still unknown, though environmental exposures, genetic predisposition, and infectious agents have been investigated as potential causes. The document provides background on the epidemiology, clinical manifestations, staging, and pathophysiology of MF and SS. It also discusses current treatments for CTCL including histone deacetylase inhibitors.
Atlas of salivary gland pathology
This document is an atlas describing the pathology of salivary gland neoplasms. It begins with chapters on the gross and microscopic anatomy of salivary glands. Subsequent chapters provide detailed descriptions and photomicrographs of various benign and malignant salivary gland tumors, organized by histologic type. The goal of the atlas is to aid in the diagnosis of salivary gland neoplasms for pathologists and head and neck specialists.
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...National Institute for Cellular Biotechnology
First ESACT Frontiers Retreat, 22-26th October, 2016 (Lyon, France) Program & Abstract Book
The European Society for Animal Cell Technology (ESACT) brings together scientists, engineers and other specialists working with animal cells in order to promote communication of experiences between European and International investigators and progress the development of cell systems and their derived products. ESACT was founded in 1976 to create a forum for the exchange of ideas on biological and engineering techniques to promote knowledge and the use of human and animal cells e.g. for the manufacturing of products. Members include scientists and engineers in academic, medical and industrial R&D and Production at applied science institutions and universities, in the medical services, in industry, and in the political and regulatory bodies. ESACT actively encourages interactions between academia, governmental and policy making agencies and the manufacturing and service industries.
Integrin Signalling And Cancer Front Pgs
This thesis submitted by Erik Freiter examines the role of integrin signaling and Src tyrosine kinase in cytoskeletal rearrangement during melanoma metastasis. Freiter hypothesized that Src activity reduces RhoA-mediated cytoskeletal rearrangement. Using cultured metastatic melanoma cells and primary melanocytes, Freiter altered Src activity exogenously. He observed an increase in GTP-bound RhoA when Src activity was decreased but no change in GTP-bound Rac1. The data suggest RhoA is involved in cytoskeletal remodeling downstream of Src in melanoma cells. Src may activate the regulator p190RhoGAP, reducing GTP-bound RhoA levels. Rac1 did not appear to be activated by Src in this model.
Similar to Counting immunoassay (CIA) (20)
Revision questions on 1.6. Cell division of IB Biology
Revision questions on 1.6. Cell division of IB Biology
(SpringerBriefs in Public Health) Duncan Dartrey Adams, Christopher Dartrey A...
(SpringerBriefs in Public Health) Duncan Dartrey Adams, Christopher Dartrey A...
Pathophysiology a practical approach (Lachel Story) (z-lib.org).pdf
Pathophysiology a practical approach (Lachel Story) (z-lib.org).pdf
[] Molecular cancer_therapeutics_strategies_for_d(book_zz.org)
[] Molecular cancer_therapeutics_strategies_for_d(book_zz.org)
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...
Program & Abstract Book: First ESACT Frontiers Retreat, 22-26th October, 2016...
More from EchoHan4
ADC conjugate sites analysis
Antibody-drug conjugates employ the specific monoclonal antibodies (mAbs) to achieve targeted delivery of the conjugated cytotoxic molecules to tumor cells.
https://www.creative-biolabs.com/adc/conjugate-sites-analysis.htm
adc chemistry
As one major component of an antibody-drug conjugate (ADC), the antibody is the key for target specificity and serves as the cargo to deliver the cytotoxic drug (payload).
https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm
ADC Biotechnology
To retain antibody bioactivity, mild, near-physiological conditions are often used for conjugation reactions. Under these conditions, endogenous amino acids such as Lys and Cys are chemically reactive and can be used as conjugation sites.https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm
Antibody Design and Conjugation
As one major component of an antibody-drug conjugate (ADC), the antibody is the key for target specificity and serves as the cargo to deliver the cytotoxic drug (payload). A payload drug can be attached to different sites on an antibody using diverse conjugation chemistry. Multiple endogenous amino acids can serve as potential conjugation sites. However, to achieve more precisely controlled site-directed conjugations and subsequently a narrower distribution of drug-to-antibody ratio (DAR), special moieties with unique conjugation chemistries are engineered into antibody sequences in our antibody design services.
https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm
adc cd40
CD40, also known as TNFRSF5, is a type I transmembrane protein. The molecular weight of CD40 is 48-kDa and it consists of a 193 amino acid (aa) extracellular domain, 21 aa leader sequence, 22 aa transmembrane domain, and a 62 aa intracellular domain in human (90 aa in mouse).https://www.creative-biolabs.com/adc/target-cd40-122.htm
ADC Biochemical Analysis
ADC preparation involves the chemical conjugation of the three components and depending on the conjugation strategy used, this process often yields complex and heterogeneous products.https://www.creative-biolabs.com/adc/adc-biochemical-analysis.htm
ADC Antibody Screening
While conventional cancer therapies (surgery, chemo therapy, and radiation therapy) have shown some success in the battle again cancer, they are often accompanied by complex and sometimes, severe side-effects due to the lack of target specificity. To circumvent this flaw and improve the efficacy and safety of cancer treatment, targeted cancer therapies, especially antibody-drug conjugates (ADCs), have been actively exploited and they are gaining a significant amount of attention during the recent years.https://www.creative-biolabs.com/adc/adc-antibody-screening.htm
ADC Affinity Measurement
The elegant design of an antibody-drug conjugate is designated to achieve targeted delivery of the conjugated cytotoxic agents to tumor cells and drug release upon antigen binding and internalization, thus maximizing the antitumor effects while minimizing cytotoxicity to normal tissues. The efficacy of an ADC greatly depends on the specific antigen binding activities of the monoclonal antibody (mAb) portion of the molecule.https://www.creative-biolabs.com/adc/adc-affinity-measurement.htm
5t4 adc
Development of 5T4-based Bispecific ADCs
A bispecific antibody can bind two different targets or two distinct epitopes on the same target. 5T4, specifically overexpressed on the cell surface of various tumors and internalized rapidly when bound to antibody, may be used as an attractive target to develop effective immunotherapy such as bispecific antibody-drug conjugate (ADC).
https://www.creative-biolabs.com/adc/development-of-5t4-based-bispecific-adcs.htm
IND Publishing and Submission
✔ Registration with CTSC
✔ Preparing IND package including Cover letter, IND, 1571, 1572 form and certification form 3674.
✔ Assembling and binding volumes
✔ Submission
https://www.creative-biolabs.com/immuno-oncology/ind-publishing-and-submission.htm
Pre-IND Meeting
For the drug development, pre-IND meeting is a critical tool to discuss the needs and challenges specific to the general product development, nonclinical testing, manufacturing information, protocol design or other regulatory questions defined in the Code of Federal Regulations (21 CFR 312.82). https://www.creative-biolabs.com/immuno-oncology/pre-ind-meeting.htm
Medical Writing and Translation
✔ Clinical overviews (eCTD Module 2.5) including literature review and references
✔ Clinical summaries (eCTD Module 2.7) including clinical pharmacology, efficacy, and safety
✔ Clinical study report preparation and review (eCTD Module 5)
✔ Clinical justification documents for EU, US and other emerging Regulatory markets
✔ Gap analysis for dossiers in clinical module
✔ Clinical and nonclinical document support, handling queries during HA meetings and responding to them
✔ Technical review dossiers
✔ Biowaiver support and justification document services
https://www.creative-biolabs.com/immuno-oncology/medical-writing-and-translation.htm
Regulatory Strategy Consulting
To gain approval for clinical testing after finalizing the pre-clinical testing of innovative new therapies, it is a key milestone for pharmaceutical companies to apply for approval of Investigational New Drug (IND) with FDA or other agencies.https://www.creative-biolabs.com/immuno-oncology/regulatory-strategy-consulting.htm
IND Regulatory Services
Navigating the drug development process from early stage discovery to clinical stage is complex and expensive. https://www.creative-biolabs.com/immuno-oncology/ind-regulatory-services.htm
cGMP Manufacturing
In the United States, the Current Good Manufacturing Practice (cGMP) is the Food and Drug Administration (FDA) 's formal regulations on the design, monitoring, control and maintenance of manufacturing processes and facilities.https://www.creative-biolabs.com/immuno-oncology/cgmp-manufacturing.htm
Antibody and Protein Toxicology
Pre-clinical toxicology is a study of the toxic effects of drugs in development based on statistical and quantitative analysis. https://www.creative-biolabs.com/immuno-oncology/antibody-and-protein-toxicology.htm
Antibody and Protein Pharmacology
Pharmacology is a key component concerned with the study of drug action in animal models which is essential and determinant to IND approval and ultimate NDA approval for a drug candidate. https://www.creative-biolabs.com/immuno-oncology/antibody-and-protein-pharmacology.htm
More from EchoHan4 (20)
Recently uploaded
National Security Agency - NSA mobile device best practices
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
Imagine a world where machines not only perform tasks but also learn, adapt, and make decisions. This is the promise of Artificial Intelligence (AI), a technology that's not just enhancing our lives but revolutionizing entire industries.
June Patch Tuesday
Ivanti’s Patch Tuesday breakdown goes beyond patching your applications and brings you the intelligence and guidance needed to prioritize where to focus your attention first. Catch early analysis on our Ivanti blog, then join industry expert Chris Goettl for the Patch Tuesday Webinar Event. There we’ll do a deep dive into each of the bulletins and give guidance on the risks associated with the newly-identified vulnerabilities.
5th LF Energy Power Grid Model Meet-up Slides
5th Power Grid Model Meet-up
It is with great pleasure that we extend to you an invitation to the 5th Power Grid Model Meet-up, scheduled for 6th June 2024. This event will adopt a hybrid format, allowing participants to join us either through an online Mircosoft Teams session or in person at TU/e located at Den Dolech 2, Eindhoven, Netherlands. The meet-up will be hosted by Eindhoven University of Technology (TU/e), a research university specializing in engineering science & technology.
Power Grid Model
The global energy transition is placing new and unprecedented demands on Distribution System Operators (DSOs). Alongside upgrades to grid capacity, processes such as digitization, capacity optimization, and congestion management are becoming vital for delivering reliable services.
Power Grid Model is an open source project from Linux Foundation Energy and provides a calculation engine that is increasingly essential for DSOs. It offers a standards-based foundation enabling real-time power systems analysis, simulations of electrical power grids, and sophisticated what-if analysis. In addition, it enables in-depth studies and analysis of the electrical power grid’s behavior and performance. This comprehensive model incorporates essential factors such as power generation capacity, electrical losses, voltage levels, power flows, and system stability.
Power Grid Model is currently being applied in a wide variety of use cases, including grid planning, expansion, reliability, and congestion studies. It can also help in analyzing the impact of renewable energy integration, assessing the effects of disturbances or faults, and developing strategies for grid control and optimization.
What to expect
For the upcoming meetup we are organizing, we have an exciting lineup of activities planned:
-Insightful presentations covering two practical applications of the Power Grid Model.
-An update on the latest advancements in Power Grid -Model technology during the first and second quarters of 2024.
-An interactive brainstorming session to discuss and propose new feature requests.
-An opportunity to connect with fellow Power Grid Model enthusiasts and users.
Building Production Ready Search Pipelines with Spark and Milvus
Spark is the widely used ETL tool for processing, indexing and ingesting data to serving stack for search. Milvus is the production-ready open-source vector database. In this talk we will show how to use Spark to process unstructured data to extract vector representations, and push the vectors to Milvus vector database for search serving.
Introduction of Cybersecurity with OSS at Code Europe 2024
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
20240607 QFM018 Elixir Reading List May 2024
Everything I found interesting about the Elixir programming ecosystem in May 2024
Digital Marketing Trends in 2024 | Guide for Staying Ahead
https://www.wask.co/ebooks/digital-marketing-trends-in-2024
Feeling lost in the digital marketing whirlwind of 2024? Technology is changing, consumer habits are evolving, and staying ahead of the curve feels like a never-ending pursuit. This e-book is your compass. Dive into actionable insights to handle the complexities of modern marketing. From hyper-personalization to the power of user-generated content, learn how to build long-term relationships with your audience and unlock the secrets to success in the ever-shifting digital landscape.
20240609 QFM020 Irresponsible AI Reading List May 2024
Everything I found interesting about the irresponsible use of machine intelligence in May 2024
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
A Mix Chart displays historical data of numbers in a graphical or tabular form. The Kalyan Rajdhani Mix Chart specifically shows the results of a sequence of numbers over different periods.
Programming Foundation Models with DSPy - Meetup Slides
Prompting language models is hard, while programming language models is easy. In this talk, I will discuss the state-of-the-art framework DSPy for programming foundation models with its powerful optimizers and runtime constraint system.
GraphRAG for Life Science to increase LLM accuracy
GraphRAG for life science domain, where you retriever information from biomedical knowledge graphs using LLMs to increase the accuracy and performance of generated answers
HCL Notes and Domino License Cost Reduction in the World of DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
Best 20 SEO Techniques To Improve Website Visibility In SERP
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
Artificial Intelligence for XMLDevelopment
In the rapidly evolving landscape of technologies, XML continues to play a vital role in structuring, storing, and transporting data across diverse systems. The recent advancements in artificial intelligence (AI) present new methodologies for enhancing XML development workflows, introducing efficiency, automation, and intelligent capabilities. This presentation will outline the scope and perspective of utilizing AI in XML development. The potential benefits and the possible pitfalls will be highlighted, providing a balanced view of the subject.
We will explore the capabilities of AI in understanding XML markup languages and autonomously creating structured XML content. Additionally, we will examine the capacity of AI to enrich plain text with appropriate XML markup. Practical examples and methodological guidelines will be provided to elucidate how AI can be effectively prompted to interpret and generate accurate XML markup.
Further emphasis will be placed on the role of AI in developing XSLT, or schemas such as XSD and Schematron. We will address the techniques and strategies adopted to create prompts for generating code, explaining code, or refactoring the code, and the results achieved.
The discussion will extend to how AI can be used to transform XML content. In particular, the focus will be on the use of AI XPath extension functions in XSLT, Schematron, Schematron Quick Fixes, or for XML content refactoring.
The presentation aims to deliver a comprehensive overview of AI usage in XML development, providing attendees with the necessary knowledge to make informed decisions. Whether you’re at the early stages of adopting AI or considering integrating it in advanced XML development, this presentation will cover all levels of expertise.
By highlighting the potential advantages and challenges of integrating AI with XML development tools and languages, the presentation seeks to inspire thoughtful conversation around the future of XML development. We’ll not only delve into the technical aspects of AI-powered XML development but also discuss practical implications and possible future directions.
Recently uploaded (20)
National Security Agency - NSA mobile device best practices
National Security Agency - NSA mobile device best practices
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
Building Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and Milvus
Introduction of Cybersecurity with OSS at Code Europe 2024
Introduction of Cybersecurity with OSS at Code Europe 2024
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying Ahead
20240609 QFM020 Irresponsible AI Reading List May 2024
20240609 QFM020 Irresponsible AI Reading List May 2024
Nordic Marketo Engage User Group_June 13_ 2024.pptx
Nordic Marketo Engage User Group_June 13_ 2024.pptx
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
GraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracy
HCL Notes and Domino License Cost Reduction in the World of DLAU
HCL Notes and Domino License Cost Reduction in the World of DLAU
WeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation Techniques
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
Counting immunoassay (CIA)
- 1. Bioassays for Antibody and Protein ADCC, CDC, and ADCP…………………..………………............................. Immunoassay………………………………….….…....................................... Immune Checkpoint Assay………………….………………………………. FcR Binding Assay………………….……………………………………………. Tissue Cross-Reactivity Studies……………….….……………………….. Immune Cells Function Assay……..…….…..…………………………….. Immuno-Oncology Assay……..…….…..…………………………………... Contamination Testing……..…….…..……………………………………….. Protein Characterization……..…….…..…………………………………….. Viral Clearance Studies……..…….…..………………………………………. Host Cell Protein(HCP) Analysis…..……….….................................... Biosafety Testing……………………………………...................................... Analytical and Bioassay Quality Control….................................. Custom Antibody Bioassays Service….......................................... CONTENTS 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Designed Just for You To ensure the best quality of our antibody and protein bioassays service , Creative Biolabs has developed several platforms supported by our structure analysis scientists. In addition, we also have quality control lab, molecular biology lab, etc. Creative -biolabs Creative Biolabs Confidential 3