Exercise 8 Histologie Preparelion of Tissues Read the stepe for the hi.docx
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Exercise 8 Histologie Preparelion of Tissues Read the stepe for the histologic preparation of tisues in Chapter 5 of the texbbook, Mateh the steps below to the corresponding photograph. There are fewer photos than there are steps, to not all of the steps will have matchies. Stee 1: Tissue is ploced in a fucativo selution wech ou loimetin. The ratio of the volume of formalin to the volume of lissue is 10: 1. This slep is coliod fixation. Siep 2. Tissues are examined, sectioned, and ploced in car 1efles. Which ore numbered for identicicalien. They are then placed in an oulomalic lissuo procestor to be hoatod Elup 5: Using a apeciol eveling device colad a micolone, Siop 6 : Severd alices ave compleied serialy, ferming a pooe. Thin skip, which is corerily ploced on the surbes of a evele: boin ovoraight. Undor a vocuum systam, the fissubs a'e detyr dratod and infillogled with paraflin: Slep 3: The lissue cossethes are remaved from the procossor Siep 7: Careluly, the thin secient ore colecind ondb bteied gloss microvepes ilides: Siep B) The alides ore voralened to a warm incubator, which tpeedi the drying process and hepe the posalinitited fasue io odhare to the gions side Slap 9. The Itwes ove ravelered to a matine that deporat: and the contents nansterred to rectangulat matal molds. finimes and tehydroles them so that oq oous kgins cus tood With thom. They ore then alained and kesequenty dehy which are flled with liquid paraffin. The paraffin hardans dioled again. Into a soit white wax, Slep 4: Just before being sliced with ice to focilitate slicing Shep. 10: A snali onount of Pentourt and a prolestive cover slip are applied lo eoch side. Step. 11. Finuhed sides are logged in a pounal betore being examined mieroteopicoly by a veterinary pohalogy Step Step. Step Stcp .
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