2. INTRODUCTION
The Vascular Adhesion Molecule-1 (VCAM-1) is a cell surface glycoprotein expressed by
cytokine-activated endothelium, mediates the adhesion of monocytes and lymphocytes.
Several polymorphisms were identified in the promoter region of the VCAM-1 gene that affects
its expression levels and many of these polymorphisms were suggested to be associated
within range of inflammatory diseases .
High serum levels of V-CAMP-1 in patients with
T2DM may support the role of inflammation in
the pathogenesis of type 2 diabetes.
3. INTRODUCTION
Diabetes mellitus : common global health problem
metabolic disorder inflammation reflecting response
common genetic variants + environmental factors like older age,
O Obesity and family history of diabetes
Single nucleotide polymorphisms (SNPs) are defined as loci with alleles that
differ at a single base, with the rarer allele having a frequency of at least 1% in
a random set of individuals in a population.
4. AIM
The aim of this study was to examine the genotype of VCAM-1 gene
polymorphisms (rs3783605 A>G) and its association with T2DM in Iraqi patients.
5. Materiales y métodos
129 sujetos Iraqis
68 pacientes (42 and 26 ) 61 sujetos sanos (31 and 30 )
diagnosticados con T2DM
6. Materiales y métodos
■ Extracción DNA:
La extracción consiste en el
aislamiento y purificación de
moléculas de
ADN y se basa en las
características fisicoquímicas
de la molécula.
Búsqueda de
modificaciones génicas
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7. Materiales y métodos
PCR:
La reacción en cadena de
la polimerasa, o PCR,
Se trata de una técnica
usada para crear un gran
número de copias de un
segmento de ADN, que
utiliza ciclos de
desnaturalización,
apareamiento con
cebadores y extensión por
una ADN polimerasa
termoresistente.
Mediante el uso de la PCR se
puede determinar el genotipo
de un individuo para un
polimorfismo dado, como un
microsatélite o un SNP (single
nucleotide polymorphism).
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12. Results
■ VCAM-1 polymorphism analysis showed a higher frequency of A/G
genotype in control group than patients group {100% vs. 88.2%}, that
showed statistical significance (p=0.007)
■ The [Table/Fig-5] shows significant differences (p≤ 0.05) between the
patients and control in all tested clinical features (BMI, FBS, HbA1c,
HDL, LDL, VLDL, TG and Abdominal Circumference)
■ There was a significant difference between A/G genotype patients
group and the G/G genotype patients group (p≤0.05) in all clinical
tests results.
13. DISCUSSION
Author Concept YES OR
NO?
-Rashid A, Haider I.
-Sheikhpour R, Sadeghian F,
Rajabi S,et al.
“Both male and female diabetic patients have
elevated levels of FBS, HbA1c, and lipid
profile”
-Gorlova OY, Ying J, Amos CI,
Spitz MR, Peng B, Gorlov IP.
-Lachance J, et al.
“Risky alleles are mostly derived, low-
frequency (i.e., minor) variants”
Ganz ML, Wintfeld N, Li Q,
Alas V, Langer J, Hammer M,
et al.
“other studies found a strong and
independent association between BMI and
the risk of being diagnosed with T2DM”
Yao M, Wu Y, Fang Q, Sun L, Li
T, Qiao H.
Tsuchiya T, Schwarz PEH,
Bosque-Plata L del, Geoffrey
Hayes M, Dina C, Froguel P, et
al.
Many SNPs in different genes were studied
and found to be associated with the
occurrence and development of T2DM
14. Conclusions
■ DMT2 is an example of how genetics interacts
with many environmental factors producing
important variation in DNA sequences (epigenetics)
that causes suceptibility to develop certain diseases.
■ There is a high prevalence of A/G genotype.
