Biodegradable polymer Matrix Nanocomposites for Tissue Engineering
Magnetic Freeze Casting Presentation
1. Magnetic Freeze Casting with Surface
Magnetized Hydroxyapatite for
Bioinspired Bone Implants
CINDY AYALA
BIOENGINEERING REU
DEPARTMENT OF MECHANICAL AND AEROSPACE ENGINEERING
ADVISOR: PROF. JOANNA MCKIT TRICK
GRAD STUDENT MENTOR: MICHAEL FRANK
2. Motivation
Osteoporosis
- causes erosion of trabecular (spongy) bone
- leads to decreased bone mass, especially in the elderly
- results in brittle bones that lead to unexpected failure
- titanium implants can lead to stress shielding
(reduction in bone density due to removal of stress from
bone by the implant)
- titanium implants often require adjustment surgeries
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[1] http://www.medguidance.com/thread/What-Causes-Osteoporosis.html
Goal
Create a porous scaffold made of bone
mineral which mimics the structure of bone
5. Procedures
• For this experiment, hydroxyapatite is used because it is the essential mineral
component of bone and teeth.
• Hydroxyapatite particles are magnetized by mixing cationic charged ferrofluid,
hydroxyapatite and water
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Magnet
12. Conclusion
• Hydroxyapatite particles (HA, 2 μm) are compared to
past work with alumina (195, 225, 350 nm).
• Bigger particle size (HA) needs lower magnetic field to
align more particle chains in scaffold center.
• Stiffness (Young’s Modulus) is enhanced when more
particle chains are aligned in scaffold center.
• 20 vol% HA (≈75% porosity) at 25 mT was best condition.
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Scaffold
X-section
Scaffold
Center Cube
Particle
Chains
Goal
More particle chain alignment in
scaffold center rather than at poles
13. Future Work
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• Take best condition (20 vol% HA, ≈75% porosity, 25 mT) and do further work to strengthen scaffold.
• Wrap scaffold cylinder with a biodegradable thermopolymer (polylactic acid, PLA) to resemble impact resistant
porcupine quill that is a keratin foam wrapped with a keratin cortex sheath.
• Infiltrate scaffold pores with a biodegradable polymer (polyethylene glycol diacrylated, PEGDA), photoinitiator
and phosphate binding element (calcium acetate) and then UV cure to crosslink PEGDA within HA scaffold.
• Compare overall mechanical properties of reinforced HA scaffold implant in axial and radial (Brazilian Test)
compression to assess viability of structural design for spongy bone biomedical implants.
14. Acknowledgements
•Professor McKittrick for welcoming me into her team
•PhD student Michael Frank for mentoring me through this project
•Fellow undergrads Sze Hei Siu, Louis Guibert, and Joyce Mok for assisting me in my research
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Editor's Notes
Superparamagnetic magnetite nanoparticles (≈ 10 nm) within cationic charged ferrofluid were electrostatically absorbed onto the surface of hydroxyapatite particles (≈ 2 µm) by mixing in water. Slurries of 10 and 20 vol % magnetized hydroxyapatite were then subjected to a low magnetic field (25 or 50 mT) during magnetic freeze casting. Subsequent freeze drying and sintering steps produced scaffolds with pores where ice crystals had previously grown in a uniform direction