1. Adjuvant effect and TLR4 mediated activation of
macrophages by polysaccharide from Polyporus albicans
Author: Antik Bose
Senior Scientist and Project Advisor, Fred Hutchinson Cancer Research
Center, 1100 Fairview Avenue N, Seattle, WA 98109.
Abstract
A water soluble polysaccharide from mycelium of Polyporus albicans has been
identified for its potency in stimulating cellular and humoral immune response.
The adjuvant potential of this polysaccharide was identified and found to be
higher than conventional adjuvants. The 37 KDa polysaccharide was purified using
different macroporous absorption resins and Reverse phase HPLC. Structural
determination was done by sugar and methylation analysis, 13
C NMR, FTIR,
MALDI-TOF,ESI-IT, MALDI-PSD TOF spectroscopy. The molecular size of the
polysaccharide was determined by Zetasier nanosystem. It showed TLR4
dependent NO, IL-1β and TNF-α production.