1. TM
Enzymes are proteins that catalyze biological
reactions, lowering energy barriers to achieve
reaction efficiency. For every chemical reaction
that occurs in life, there's an enzyme that
specializes in making that process quick and easy.
An enzyme "finds" its target in complex mixtures,
using a highly specialized active site. We’ve
simply harnessed the natural enzymatic
mechanisms to be used for analytical chemistry.
Enzymes offer precision, specificity, sensitivity,
reliability, safety, and ecological stewardship for
analytical chemistry by replacing toxic chemicals
and methods involving high heat or pressure.
NECi Superior Enzymes has been designing
recombinant enzymes for analytical chemists
since 1993. We develop robust assays for
industrial quality assurance, agriculture,
academia, environmental research, regulatory
monitoring, citizen science, and more.
Cofounder of NECi Superior Enzymes, Bill
Campbell, is a renowned biochemist and
recombinant protein design expert.
Dr. Campbell’s research on nitrate reductase
resulted in numerous peer-reviewed scientific
journal publications along with various research
grants and awards to continue develop enzyme
based analytical assays and test kits.
We design recombinant proteins in order to
develop robust assays that
deliver precise & accurate results
without the use of toxic reagents
Why Enzymes? Why Us?
SPECIFIC PRECISE SENSITIVE RELIABLE NON-TOXIC
The enzymatic
active site only
binds nitrate -
eliminating
potential
interferences,
false positives and
false negatives.
Enzymes only
target their
analyte, even in
complex sample
matrices, offering
reproducible
results.
Enzymes offer
low detection
limits with
minimal
interferences,
finding even the
smallest amount
of the target
analyte.
Enzymes only react
with target analyte
in a small reaction
volume, ensuring
data accuracy in
these robust
analytical methods.
Enzymes are
biological proteins
and react under
gentle conditions
for safe and clean
analysis. Small
aqueous reaction
volume minimizes
reagent waste.
BENEFITS OF REAGENT GRADE ENZYMES FOR ANALYTICAL CHEMISTRY
ENZYMES FOR ANALYTICAL CHEMISTRY
334 Hecla Street, Lake Linden, MI 49945
info@nitrate.com | 1.906.296.1130
sales@nitrate.com | 1.906.296.1115
www.nitrate.com

2. HOW THE ENZYMATIC NITRATE ASSAY WORKS
ANALYTICAL ENZYME TEST KITS + PRODUCTS
ON-SITE TEST KITS LABORATORY TEST KITS ENZYME REAGENT PACKS
Easy to perform, no
laboratory or training
required for accurate on-site
analysis anywhere
Supplied with all necessary
packaged pre-measured
reagents
Handheld photometer
provides digital results on
mobile Android devices
Kits for water, soil, plant, &
forage extracts in 5, 10, 25,
or 50 sample packs
Supplied with pre-measured
reagents ready to reconstitute
Easy to follow complete
assay guide included
Robust assay available in
microplate, test tube, & NADH
disappearance formats
Optimized for laboratories
with basic supplies such as
pipettes, ice, d-I water, a
spectrophotometer, &
graduated cylinders
Ideal for laboratories with
in-house reagent preparation
capabilities
Optimized reagent packs for
method development,
automated instruments,
Competitive cost per test
Choose from 1, 2, 3, or 5 units
per vial, or 1 unit + optimized
NADH aliquot
TM
334 Hecla Street, Lake Linden, MI 49945
info@nitrate.com | 1.906.296.1130
sales@nitrate.com | 1.906.296.1115
www.nitrate.com
Place orders online at www.nitrate.com/products