23. What is the number one method of infection transmission? Improper use of personal protective equipment (PPE). Contagious respiratory diseases. Contaminated water. Hand contact. 24. Alcohol-based hand sanitizers are not recommended after contact with which organism(s)? Gram-negative bacteria. Norovirus. Spore-forming bacteria such as Clostridium difficile (C. diff) and Bacillus sp. Methicillin-resistant Staphylococcus aureus (MRSA). 26. When doffing gowns, gloves and masks, why are gloves taken off first? Because they are the most contaminated. To avoid the possible effects of skin allergies due to prolonged skin contact. Because they were put on last. To make the removal of the other items safer and easier..