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1.    A diagram was provided that showed part of a system designed to meet all basic needs of a
      family of four living in the tropics. The system was based in part on the generation of fuel gas
      by bacterial fermentation of urine and faeces.
      (a)   Candidates were asked to describe how processes which naturally formed part of the
            nitrogen cycle could make nitrogen contained in urine and faeces available to crop plants.
            There were many competent answers that showed detailed knowledge of the relevant
            parts of the nitrogen cycle and scored full marks. There were also very weak accounts,
            however, that included unselective descriptions of the whole of the nitrogen cycle
            including inappropriate references to denitrification and nitrogen fixation. The average
            type of response tended to include one or other of the following faults: the assertion that
            ammonia was present in urine or faeces; use of the term “nitrogen” in a very loose way
            with little reference to specific forms; errors in the sequence of nitrite and nitrate
            production; confusion in the nomenclature of the microorganisms involved; lack of
            precision concerning uptake of nitrate by plants.


      (b)   In the system, solid waste from the fermenter was used as fertiliser for crop plants and
            candidates were asked to explain the advantage of growing leguminous plants such as
            groundnuts or beans. It was evident that many candidates were unfamiliar with
            leguminous plants. Answers were often quite weak, with scoring limited to mention of
            root nodules (root “noodles” were held to be nutritious, but unacceptable here) and
            nitrogen fixing bacteria (with the latter frequently misidentified). There was widespread
            belief that nitrate was the product of nitrogen fixation and, in some cases, that the plant
            itself fixed the nitrogen. Few candidates appreciated that remains of the legumes would
            need to be decomposed before releasing material that could be converted to nitrates and
            consequently there were many vague answers such as “legumes return goodness to the
            soil”. Ironically, some of those who had discussed nitrogen fixation entirely out of
            context in part (a) did not repeat this material which was now relevant.


            The question concluded by asking candidates to explain the advantage in the system of
            stocking the pond with fish that feed on algae rather than with carnivorous fish. Most
            candidates chose to refer, sometimes at undue length, to prevention of eutrophication.
            Very few, however, discussed trophic levels in relation to relative energy loss but some
            did suggest that stocking with fish that feed on algae would produce a greater yield of
            fish. A large number of obscure answers suggested that carnivorous fish would need to be
            fed by “bringing meat in” and there was even the occasional idea that the cattle and
            chickens would be fed to these fish.




2.    There was a great deal of confusion between nitrogen fixing and denitrifying bacteria in part
      (a). Candidates are still not clear about the sequence of changes in the nitrogen cycle. Some
      failed to read the question carefully and began with nitrogen gas in the atmosphere rather than
      nitrogen compounds in dead spruce seeds. Weaker candidates still fail to define the compound
      containing the atom of nitrogen.




Hyde Clarendon Sixth Form College                                                                1
Decomposition or ammonification releasing ammonia from organic sources of nitrogen still
      seems to be the weak link. Candidates frequently misread part (b), and often suggested that
      moss must contain nitrogen fixing bacteria in their roots. Many spotted that competition was
      involved but were unsure of the factor for which the organisms were competing. Again,
      competing for nitrogen was a common error.




3.    The essays indicated that most candidates had given thought to organising material into a logical
      and coherent account. The Quality of Language was considerably better and there was
      substantially less of the colloquial expression that marred many essays in previous years. The
      main criticism in this area was the widespread failure to present new ideas in discrete
      paragraphs. However, as was the case last year, the scientific content of the essays proved to be
      disappointing. Work was often extremely superficial and not infrequently failed to reflect the
      detail expected of candidates following an A Level course of study.


      Relationships between animals and plants
      This was the least popular of the essays and was often chosen by the weaker candidates. As a
      result, many responses offered no more than a brief discussion of animal dependence on food
      and oxygen from plant photosynthesis before embarking on lengthy and often anecdotal
      accounts of the provision of habitats, shelter and building materials. Only rarely did such
      accounts offer an appropriately detailed discussion of trophic levels, energy transfer and carbon
      dioxide/oxygen balance. Where nutrient cycling was introduced, the better candidates
      successfully established the link between animal excretion, decomposition and the uptake of
      mineral ions by plants. Many less able candidates lost sight of the question at this point and
      essays digressed into discussions of the roles of various micro-organisms. Other areas which led
      to the introduction of much peripheral matter were references to pharmaceutical compounds
      derived from plants and to habitat destruction. Some accounts were totally irrelevant and
      suggested that candidates had misinterpreted the question as requiring a discussion of the
      similarities and differences between animals and plants.




4.    In part (a) it was obvious that some candidates had actually done this sort of exercise as they
      described in detail how random numbers are generated and used with a grid on the ground to
      place the quadrats. A minority of students still described throwing random quadrats but this
      method is prone to bias and is not random. The most common error was to omit the method of
      generating random numbers.


      Many wrote at length in part (b)without focusing on the stages of heather growth given in the
      diagram, or failed even to offer an indication of the heather’s age, and vaguely used the terms
      ‘early’ and ‘late’. The diversity of heather and not of the whole community was a popular
      misunderstanding. Some candidates agreed that the diversity would change but failed to say
      whether it would get greater or less. Although many did use the information in the table to
      explain that increased cover offered by the heather, blocked light and affected diversity. Many
      offered other factors such as more nutrients in the soil, but could not easily rationalise how that
      occurred.




Hyde Clarendon Sixth Form College                                                                 2
In part (c)(i) some candidates did manage to calculate the rate but this caused problems for
      many. Some demonstrated misunderstandings with the correct notation of SI units. Few read the
      question in part (c)(ii)so many did not relate age of the heather to the changing distribution of
      green shoots and older woody ones. The most common incorrect response involved a discussion
      of the death of parts of the plant. In part (iii) the availability of extra food for grouse was
      frequently given, but few related the fact that burning heather eventually increased cover, which
      provided the grouse with somewhere to hide. In part (d)(i) many students failed to show an
      understanding of the term ‘organic’. This can be the only explanation for responses such as
      ammonia, soil, humus, bone and root. Some however did offer protein and few even gave DNA.
      Candidates really had a problem with (d)(ii) as phosphate, sodium, and hydrogen ions were
      given, this suggested that the candidates read the question as “name an ion” rather than “name
      an ion containing nitrogen”. Ammonia and the correct named ion, but with an incorrect
      chemical formula, were common mistakes.


      In part (e) there was still a great deal of confusion over the role of the bacteria and the sequence
      of the nitrogen-containing compounds. The relative positions of ammonium, nitrite and nitrate
      ions were regularly switched. Many candidates did, however, correctly name the bacteria
      involved in the nitrogen cycle and gave complete and accurate accounts. Saprobiotic bacteria
      were rarely mentioned and often the pathway began with the ammonium ions, but with no
      mention as to how they were created from organic nitrogenous compounds. Inappropriate
      bacteria such as the nitrogen-fixing bacteria were often mentioned, candidates being unable to
      identify the relevant parts of the nitrogen cycle and so found it necessary to include everything
      they knew.


      In part (f) most candidates realised that the table showed changing amounts of nitrogen in the
      soil and heather plants, but they were at a loss to explain the reason for burning heather after 8
      rather than after 15 years. Few realised the significance of creating gaseous oxides of nitrogen
      during burning and thought that burning would place nitrogen directly into the soil.




5.    (a)   There was a strong centre bias to this part of the question. Candidates were either familiar
            with the ways in which organisms were classified or their answers appeared t6 be based
            largely on guesses.


      (b)   In part (i) most candidates appeared to be of the opinion that digestive enzymes were
            present in worm casts so that digestion could continue in the soil. Some were of the
            opinion that, as worms were primitive animals, they had short guts. They therefore
            ingested the casts and passed them through the gut a second time. There were relatively
            few references to the key points that the enzymes were not themselves digested and being
            proteins they were too large to be absorbed through the gut wall. Some of the answers to
            part (ii)showed a good understanding of the basic principles of experimental design, but
            far too many simply assumed that demonstration of the presence of reducing sugar or the
            absence of starch in worm casts proved the presence of amylase.




Hyde Clarendon Sixth Form College                                                                 3
(c)   Many candidates were content to take the lack of earthworms at face value and made no
            attempt to link it in any sensible way to the use of fungicide. Some realised that the
            information in the passage about breaking the leaves into smaller pieces was relevant, but
            few went on to develop this point further and describe the effect of increased surface area
            on the rate of microbial decomposition.


      (d)   The answers to this part of the question were generally poor. Although there were some
            references in part (i) to nitrate and ammonium ions, mineralised nitrogen was rarely
            described as being more than “nitrogen in mineral form”. The tendency to repeat the
            question also characterised many of the answers to part (ii) with excretory nitrogen
            generally described as being “nitrogen in excreted products”. Where the answer was
            amplified, excretory nitrogen was usually identified as the nitrogen found in faeces.
            Evidence from the answers to this part of the question, and elsewhere in the paper, point
            to few candidates understanding the differences between the processes of secretion,
            excretion and egestion.


      (e)   The number of times that ammonia appeared in the answer to part (i) suggested that either
            few candidates understood the meaning of the word “organic” or that ammonia was
            genuinely regarded as an example of an organic compound. Where both of the examples
            offered were organic, lipids and carbohydrates often featured. There were, in addition,
            many inappropriate references to cells and tissues. Although there were some excellent
            answers to part (ii), others reflected a fundamental lack of knowledge on the part of the
            candidates concerned or were totally unselective in the material they presented. The
            production of ammonia was often omitted, the terms “nitrifying” and “nitrogen-fixing”
            were frequently confused and nitrates were too often described as being converted into
            nitrites.


      (f)   In part (i) most candidates showed understanding that the behaviour of earthworms in dry
            conditions led to a smaller surface area over which water could be lost. Some,
            unfortunately, linked this behaviour to saving energy through a lower rate of respiration,
            while others referred to reduced rates of sweating. There were also many sound answers
            to part (ii).


      (g)   Many candidates understood the difficult concept that lowering the C : N ratio could be
            achieved either by removing carbon or by adding nitrogen. Unfortunately, the
            explanations of how these effects were achieved Were often insufficiently clear to gain
            credit. Once again, far too much use was made of unqualified pronouns and many
            members of the examining team commented on difficulties in deciding to what “it” was
            referring.




6.    Many candidates did not study the diagram of energy flow through the ecosystem with
      sufficient care.
      (a)   This was concerned with the possible fates of energy already absorbed by the producers
            but not yet transferred to organic molecules. Hence reference to energy loss by reflection
            was irrelevant, and respiratory loss was inappropriate, especially as the diagram showed
            this at a later stage.




Hyde Clarendon Sixth Form College                                                              4
(b)   In calculating the energy transfer to the primary consumers, many candidates failed to
            subtract the value for material in the producers which was not actually consumed, hence
            8760 was a more common answer than the correct 2920 kJ m –2.


      (c)   Many candidates displayed a sound grasp of the topic of nitrification by soil bacteria.
            Some spoiled their answers by giving supplementary, incorrect chemical formulae (e.g.
            “nitrate / NH3”). Some candidates included irrelevant extra material about nitrogen
            fixation and denitrification, and some had these different aspects of the nitrogen cycle
            totally confused.




7.    This question required the use of extended prose throughout. Full marks were only available to
      those able to express the appropriate concepts clearly and unambiguously in scientific terms.
      Many candidates produced good answers, using both information from the passage and their
      own biological knowledge. Lack of detail, misconceptions and careless expression all
      contributed to the loss of marks for other candidates.


      (a)   The main points given by candidates here were related to deforestation causing loss of
            habitat, loss of food, and potential extinction or the need to migrate elsewhere. Some
            gave details relating to exposure of the soil and hence erosion and leaching of ions. Very
            few mentioned changes in climate or in light levels as a result of removal of the trees.


      (b)   Many understood that trees could act as carbon sinks by absorbing carbon dioxide (but
            not ‘carbon’ as a high proportion of weak candidates stated) for use in photosynthesis and
            conversion of organic molecules within the tree. Careless omission of any of these details
            resulted in the loss of marks – the third point being the one most commonly left out. Far
            too many candidates believed that carbon dioxide was converted into oxygen or that it
            was used in ‘respiration’ rather than photosynthesis.


      (c)   Some candidates did not make it clear whether the scenario they were describing related
            to the situation where ploughing took place or, as the question stated, to where ploughing
            was reduced. Such ambiguity in the answer cost marks. Once more, ‘carbon’ was often
            the substance used rather than dead organic matter and this carbon was often thought to
            combine directly with oxygen to form carbon dioxide. Ploughing sometimes introduced
            ‘air’ into the soil, with oxygen not being specified. Similarly, the process of respiration, if
            mentioned in the context of saprotrophic organisms in the soil, was rarely qualified as
            being aerobic. Some candidates even imagined a peculiar situation whereby carbon
            dioxide was still formed in the soil but it was trapped due to a lack of ploughing.




Hyde Clarendon Sixth Form College                                                                  5
(d)   This section was specifically about the role of nitrifying bacteria. These were frequently
            confused with saprotrophic and with nitrogen fixing bacteria. For those thinking the latter,
            ploughing introduced nitrogen gas into the soil ready for fixation. Only correct, relevant
            details were rewarded, such as the conversion of ammonium ions to nitrite and then to
            nitrate, using the extra oxygen introduced by ploughing. Many candidates knew that
            nitrate ions were taken up by crop plants and some explained how these could be
            converted into named organic compounds, such as DNA or protein, which could be used
            in turn by the plant to increase its growth or yield (hence being of benefit to the crop
            plants, as required by the question). Such completeness of detail was the domain of the
            better candidates who frequently scored full marks.




8.    (a)   Weak answers followed the stem of the question and only referred to carbon rather than
            considering the form it would take in the atmosphere, namely carbon dioxide. Unclear
            responses proposed that ‘slash and burn’ would increase its concentration without
            explaining whether it was the ‘slash’, the removal of trees, or ‘burn’, the combustion of
            trees, that was responsible for the change. Not all candidates used the allocation of marks
            to construct their answer and generally included only the increase due to release of carbon
            dioxide from burning. Better candidates identified the reduced uptake of carbon dioxide
            since there would be fewer photosynthesising organisms.


      (b)   The concept tested in this question was secondary succession. Where the role of pioneer
            species was identified, clarification was needed since soil already existed from the earlier
            presence of organisms. Weak responses produced unnecessary descriptions of primary
            succession. Better candidates recognised that recolonisation would occur with seeds and
            spores from neighbouring areas although this was not always well expressed. Many
            achieved credit by explaining the process of succession and the resultant re-establishment
            of a climax community, but there were also many who appeared unfamiliar with the
            concept or who could not communicate the main ideas adequately.


      (c)   This question allowed candidates to demonstrate their ability to interpret information and
            apply their knowledge. Thus, good candidates could identify the source of ammonium
            compounds in manure, which soil bacteria would be relevant, and what was meant by
            crop yield. Many showed a good knowledge of the nitrogen cycle but only the better
            candidates considered the role of nitrogen-fixing bacteria. It was not necessary to name a
            specific nitrifying bacterium, but where this was done, the bacterium had to be linked to
            the appropriate reaction to secure credit.


      (d)   It was pleasing to see so many candidates familiar with environmental concerns and
            conservation, although candidates often failed to express themselves clearly. A large
            proportion considered the view of what would be lacking if a forest ecosystem was
            removed, rather than the advantages of conserving one, but examiners sought to credit the
            concepts. There was a limited appreciation of forests as sustainable resources but frequent
            reference to habitats, carbon sinks, species diversity and food chains allowed maximum
            credit to be obtained by many.




Hyde Clarendon Sixth Form College                                                               6
9.    (a)   Some candidates were able to gain maximum credit by linking contraction of the
            diaphragm and intercostal muscles to an increase in volume of the chest and a decrease in
            pressure, but there were many who gained little credit for their responses. Of these, some
            failed to take note of the requirements of the question and emphasised the role of
            chemoreceptors, some clearly confused inspiration with expiration, and others wrote in
            extremely simplistic terms about unspecified muscles “working” and the chest “getting
            bigger”.


      (b)   Candidates who identified part (i) as a question relating to experimental technique
            produced sound answers, commenting on the need to allow breathing rate and tidal
            volume to stabilise.
            Others responded inappropriately with such answers as “allowing the average to be
            worked out” or “warming up”. The mathematical problem in part (ii) created relatively
            few difficulties, although there were those who were uncertain of the required approach,
            or incorrectly converted the figures to cm3. The examiners had little sympathy with those
            candidates who confessed at this point that they did not have calculators. Answers to
            part (iii) were almost universally disappointing. Although candidates clearly experienced
            little difficulty with the wording of the question, they did not take sufficient care in
            picking out the underlying trends, a feature which also frequently marks their approach to
            coursework. Thus many did no more than describe an “increase in breathing rate and tidal
            volume”. Good answers needed to refer to the change in gradient.




10.   Only the better candidates were able to give satisfactory answers to the sections requiring
      understanding of the chemical aspects of the process of photosynthesis.
      (a)   Many were confused concerning the identity of the carbon dioxide acceptor substance in
            the Calvin cycle. Not many of those that did know this to be RuBP went on to stress that
            two molecules of GP would be formed per molecule of carbon dioxide fixed. The concept
            of equilibrium existing between the rates of formation and breakdown of GP was foreign
            to all but the best candidates. Many were beguiled by the superficial resemblance of the
            relevant part of the graph to a typical ‘limiting factors’ curve. Some others argued in
            favour of some sort of saturation phenomenon.


      (b)   In order to explain that the concentration of GP was increasing in the dark, many
            candidates concentrated, incorrectly, on the formation of GP rather than on its lack of
            conversion to other substances. Some even invoked GP production in glycolysis
            (forgetting that it would also have to be broken down before any ATP were produced).
            Only the most able candidates realised that conversion of GP to TP required ATP and
            reduced NADP from the lightdependent reactions and that these would, of course, soon
            be depleted in the dark.




Hyde Clarendon Sixth Form College                                                               7
(c)   Most candidates tended to be successful in their interpretation of the data in the table and
            understood that, in the light (between 8 am and 4 pm) photosynthesis would occur, hence
            removing carbon dioxide from the air and leaving a lower concentration, as shown in the
            data.
            Many also appreciated that the absence of leaves, or reduced light levels, or even the
            production of carbon dioxide by soil organisms, would result in a higher concentration of
            carbon dioxide at ground level than that found higher up among the leaves of the plants.


      (d)   Many candidates realised that the wind could alter the concentration of carbon dioxide in
            the air, but not all of these explained that this would be due to the wind bringing in fresh
            supplies or, alternatively, removing carbon dioxide. Very few candidates went on to
            explain that the wind would therefore have introduced another experimental variable and
            hence made any measurements of carbon dioxide concentration in the air (which was the
            basis of the experiment) unreliable. There was also much irrelevant discussion of the
            effect of the wind on the rate of transpiration.


      (e)   While many candidates appreciated that saprotrophic microorganisms would cause dead
            leaves to decay and hence release carbon dioxide which could then be taken up by the
            plants, fewer gave more specific details relating to the involvement of digestion and
            respiration. Many thought that ‘carbon’ would be released into soil for uptake by plant
            roots. Some could not resist the temptation to include irrelevant comments about the
            nitrogen cycle, despite carbon having been stated quite explicitly as the subject of the
            question.




11.   This question required the use of extended prose throughout. Full marks were only available to
      those able to express the appropriate concepts clearly and unambiguously in scientific terms.
      Many candidates produced good answers. Lack of detail, misconceptions and careless
      expression all contributed to the loss of marks for other candidates.


      (a)   Many candidates were very careless and gave few practical details of how sticky traps
            could be used to compare the diversity of flying insects in the two environments.
            Elementary procedures such as the use of multiple traps and details of how these would
            be placed at random were frequently overlooked. Most suggested that the number of
            individuals of each species caught by the traps should be counted and used to calculate an
            index of diversity, but many others were confused and thought that the mark-release-
            recapture technique should have been used in order to find the Lincoln Index.


            While most candidates appreciated that the diversity of insects in the forest would be
            greater than that in a cultivated field, some gave very little detail in their explanation of
            the reason for this. Better candidates included details regarding the relative harshness of
            the two environments (often giving the use of pesticides in cultivation as a factor
            detrimental to insects), and the abundance of different habitats and greater variety and
            quantity of food available in the forest. Many wasted both time and space by reiterating
            the converse argument for the second habitat rather than expressing themselves in
            comparative terms once only.


      (b)   The explanation offered for why crops could only be grown for a few years in a field
            produced by clearing part of the forest were frequently vague. References were made to
            ‘nitrogen’ ‘running out’ with no detail such as the removal of material in the crops at

Hyde Clarendon Sixth Form College                                                                  8
harvest leaving little organic matter to decay in the soil, or to leaching of soluble
             nitrogen-containing ions such as ammonium and nitrate. The better candidates had little
             problem however in applying their knowledge and understanding of the nitrogen cycle to
             explain how the concentration of nitrates in the soil could be restored with time. Some
             mentioned nitrogen fixation (both atmospheric and biological) and most gave detailed
             accounts of putrefaction and nitrification. Weaker candidates tended to concentrate on
             colonisation and succession and lost sight of any connection with nitrate production.




12.   BYA8
      Candidates gave the impression of leaving themselves adequate time for attempting the essay
      and it was encouraging to note that many were able to write three or more sides on their chosen
      topic. Some obviously encountered considerable problems in deciding which of the two essays
      to attempt and it was not uncommon to see comprehensive plans relating to both and
      sometimes, even, a substantial false start. It is important, however, for candidates to appreciate
      that length in itself is not a merit.
      Care has to be taken over ensuring that the essay is written clearly enough for it to be read by
      the examiner. This was by no means always the case. There was surprisingly little evidence of
      planning in the work from some centres, and it is possible that this was the main reason for so
      many candidates involving themselves in so much needless repetition.


      Many candidates felt the need for a lengthy introduction defining the title. Unfortunately such
      introductions were frequently devoid of significant factual content or merely served to indicate
      that the candidate had little intention of restricting the essay to relevant processes.
      The carbon cycle was generally well understood in principle but accounts frequently extended
      over one or more pages and lacked significant detail, merely referring to carbon being
      transferred.


      Candidates who had a thorough knowledge of the nitrogen cycle often produced excellent
      accounts of this topic. Others experienced considerable problems. These often stemmed from
      failing to appreciate the underlying principles. Thus it was not uncommon to encounter
      candidates who saw the whole process as a mechanism centred on maintaining the atmospheric
      concentration of nitrogen constant.
      Such candidates inevitably started their accounts with a reference to nitrogen fixation and ended
      with denitrification. There was also much confusion between the events of the different stages
      in the cycle.


      Biochemical cycles featured in many accounts and, in general, the level of factual knowledge
      here was impressive. Unfortunately many saw inclusion of this material as an opportunity to
      display a broader understanding of the processes of respiration and photosynthesis. As a result,
      they included much material which could not be considered as relevant to an essay on cycles.




Hyde Clarendon Sixth Form College                                                                9
The oestrous cycle was the best described physiological cycle and, where candidates had paced
      themselves sensibly and allowed time to incorporate detail of hormonal control, produced some
      excellent detail. Other cycles were less convincing and there were many accounts of
      physiological process such as synaptic transmission and digestion which, with the best will in
      the world, could not be considered as cyclical. A case could be made for the inclusion of other
      physiological processes such as those involving homeostasis, the circulation of blood and the
      mechanism controlling breathing, but these processes were also usually described as linear
      sequences or they lacked the necessary detail to add to the quality of the essay.


      BYA9
      The essays varied greatly in length, depth of knowledge and relevance. The most common
      failing was the inclusion of a number of cycle names without at least a paragraph about the
      cycle at the appropriate depth. Candidates should not hesitate to use small, simple sketches (e.g.
      carbon cycle, structure of glucose) to illustrate their work, provided such diagrams are labelled
      accurately. The full range of marks was used on both essays, with Essay A being less popular
      than Essay B, and there was pleasing evidence of planning, both in the form of a plan and as
      demonstrated by a piece of coherent, flowing prose. There is a requirement for technical
      language at this level so terms such as ‘kicks in’, and ‘Mother Nature’ devalued the essays in
      which they appeared. It is important that candidates realise that erroneous or unsupported
      mention of an aspect of the specification may actually detract from the impression their essay
      gives.


      Candidates found it hard to introduce this topic succinctly. The expectation was that candidates
      would select at least one each from a range of cycles from which they could emphasise the
      common feature of the continuity of a cyclical process, describing the details in sufficient depth
      and in technical language such that they gained maximum marks. Some candidates achieved
      this ideal.
      The range covered included ecological cycles (usually nitrogen and carbon cycles),
      physiological cycles (the menstrual cycle, the cardiac cycle), biochemical cycles (the Krebs and
      Calvin cycles) and parasitic life cycles (Plasmodium and Schistosoma).


      Excellent candidates described the carbon and, more particularly, the nitrogen cycle in
      considerable depth. The range of ways in which plants could obtain the nitrogen-containing ions
      they need for protein synthesis was thoroughly explained, sometimes with faultless use of the
      names of the microorganisms involved. Weaker candidates muddled the process involved,
      confusing names and introducing erroneous ideas (‘carbon is taken up through the roots’,
      ‘plants take in carbon dioxide for respiration’, ‘when plants and trees are eaten, the nitrate they
      contain…’, etc.). In some cases, cycles were named but nothing was offered to explain them at
      all.


      Again some excellent accounts of the menstrual cycle were seen, including considerable detail
      on hormones and on uterine changes, The cardiac cycle was well described by a number of
      candidates, but many used this term to mean the circulation of blood from the heart to lungs and
      back again. These answers generally offered little detail or accuracy above average GCSE level.




Hyde Clarendon Sixth Form College                                                               10
The Krebs and Calvin cycles were clearly set in context and briefly related to linked reactions
      by the better candidates. Weaker essays muddled the two processes or included lengthy (and
      irrelevant) sections on the ETC or the light-dependent reaction. It was, of course, possible to use
      cyclic photophosphorylation as a valid example of a cycle but few managed to do this
      successfully.
      Descriptions of parasitic life cycles were of variable quality. Some near perfect accounts were
      seen, but in others, particularly when describing schistosomiasis, the stages of the life cycle
      appeared to be a mystery.




13.   Many candidates found this to be quite a difficult question. Answers were frequently expressed
      with insufficient clarity and data were often used non-selectively.
      (a)   Most candidates realised that the data for the grass in graphs C and D showed no
            significant difference. Weaker candidates imagined that some of the slight differences
            were of significance.
      (b)   For those who selected the appropriate graphs for comparison (i.e. A and C, with neither
            having Rhizobium added and only C having the nitrate), there was little difficulty in
            finding evidence for the effect of nitrate fertiliser on the soybeans. Less selective
            candidates produced very confused arguments.


      (c)   This section required candidates to both describe and explain the effect of Rhizobium on
            the growth of the soybeans. Many candidates did either one or the other. Explanations
            were frequently confused regarding the details of nitrogen fixation and answers often
            included nitrate production in addition to, or instead of, that of ammonium ions. A
            sizeable minority realised that a mutualistic relationship existed between the Rhizobium
            and the legume (or that, at least, the bacterium formed nodules on the plant’s roots), and
            some went on to explain that the plant would gain organic nitrogen compounds such as
            amino acids or proteins as a result of the relationship.
            In sections (b) and (c), a sizeable proportion of candidates appeared confused regarding
            what had been used in the experiment as a measure of plant growth – there were many
            references to the ‘number of seeds’ produced but, since this was plotted on the x-axis, it
            should have been realised that the number of seeds was a manipulated variable and not a
            result.




Hyde Clarendon Sixth Form College                                                               11
14.   (a)   Some accounts of the activities of decomposers and nitrifying bacteria in the recycling of
            carbon and nitrogen from fallen leaves were truly excellent. Aspects which tended to be
            omitted were mainly those associated with the early stages of saprotrophic nutrition, i.e.
            the secretion of hydrolytic enzymes for external digestion followed by absorption of the
            products by diffusion or active transport. Details of respiration releasing carbon dioxide
            to be used by the trees in photosynthesis, of the release of ammonium compounds its
            conversion to nitrite and nitrate and the subsequent use of the latter by the trees to
            produce organic nitrogen compounds such as amino acids were frequently given.
            However, weaker candidates typically knew few of these details and often described how
            ‘carbon’ was released into the soil, in which state it was apparently taken up by the trees’
            roots. Some candidates were determined to display all they knew about the nitrogen cycle
            and included irrelevant details of denitrification and nitrogen fixation.


      (b)   For candidates who kept to the point, this was a very straightforward question: felled
            trees no longer removed carbon dioxide from the atmosphere by photosynthesis and
            burning them released more carbon dioxide back into the atmosphere – hence a predicted
            rise in the carbon dioxide concentration. Some became too involved in telling a story and
            often forgot about one or other of the key processes.


      (c)   Answers to this section were very disappointing. Many candidates merely repeated
            information given in the stem of the question but did not use this, nor did they attempt to
            apply biological principles to the situation with which they were presented. Others treated
            examiners to the answer they had prepared earlier, with sound biological facts irrelevant
            to this question. One area which was hardly ever explored was how the protective canopy
            of the softwood trees might actually have modified the environment to make it suitable for
            the growth of hardwoods beneath it – such aspects as protection from wind and
            rainstorms, provision of a humid environment, shedding their leaves making ions
            available to the hardwoods, reducing soil erosion and leaching of ions due to their root
            systems being already established in the soil. Usually answers contained a reference to
            the light penetrating following tree felling so that seeds could germinate and the idea that
            succession to a climax community would occur, and little else of any relevance. Hardly
            any candidates considered that being exposed to light might possibly be advantageous for
            photosynthesis in the seedling trees. Better candidates realised that more seeds could be
            transported into the cleared area from the adjacent forest, and that animals might return
            because new habitats and food would be available for them as the new trees began to
            grow.




15.   Once again, the nitrogen cycle proved a weak link in the understanding of a large number of
      candidates.


      (a)   More candidates were able to identify process P as denitrification than were able to
            identify process Q as nitrogen fixation. Nitrification was a common wrong answer, as
            was ammonification, since this refers to the conversion of nitrogen in organic compounds
            to ammonia.




Hyde Clarendon Sixth Form College                                                              12
(b)   Many candidates misread the questions and did not realise that they had to account for the
            apparent discrepancy between the amount of ammonia converted to nitrates and the
            amount formed by nitrogen fixation. Good candidates knew that ammonia is also formed
            by the decomposition of proteins, amino acids, urea and other organic compounds in the
            detritus that contain nitrogen.


      (c)   Most candidates realised that hydrogen is lost and oxygen is gained in the conversion of
            ammonia to nitrate.




16.   (a)   Nearly all candidates knew that habitats would be lost, and a good number also knew that
            this would reduce the number of species in the area.
      (b)   Better candidates realised that both potassium nitrate and ammonium nitrate would
            release nitrate ions into the soil immediately, and that the ammonium ions in ammonium
            nitrate could be nitrified by nitrifying bacteria to provide a secondary release of nitrates.
            Many candidates, however, just did not apply their knowledge to the problem and merely
            recited chunks of the nitrogen cycle, with some confusion between nitrifying and
            nitrogen-fixing bacteria.




17.   (a)   Most candidates knew the mark-release-recapture technique, and were able to describe
            the various steps. However, they did not always explain the reasons behind the steps. For
            example, they did not always explain that the released insects should be left for a suitable
            period of time to allow them to re-integrate with the rest of the population.


      (b)   (i)     Nearly all candidates knew that there would be only one degree of freedom.
            (ii)    Most candidates knew that the 0.05 level of probability is that most commonly
                    used in biological analysis to judge statistical significance.
            (iii)   Responses to this section were generally disappointing. Most candidates were
                    unable to reason that, because the value for χ2 is greater than the critical value,
                    then there is a probability of less than one in one thousand that the results are due
                    to chance. They were uncertain as to whether the difference in values of χ2 implied
                    that the differences in results are due to chance or due to some biological cause.
                    They wrote about rejecting a null hypothesis which had not been stated and also
                    merely that ‘the results are statistically significant’. Candidates should be aware of
                                        2
                    the logic that, if χ is greater than the critical value, there is only a probability of
                    (usually one in twenty) that the results are due to chance as the basis for rejecting
                    any null hypothesis and accepting the experimental hypothesis.


      (c)   (i)     A number of candidates realised that some of the biomass produced in
                    photosynthesis would be respired by the plant, but very few actually explained that
                    biomass is lost in the form of carbon dioxide. Most of those who involved
                    respiration in their answers suggested that energy is lost, which is true, but loss of
                    energy does not account for the difference in biomass between gross primary
                    production and net primary production.


            (ii)    Nearly all knew that a higher net primary production would lead to more dead
                    plants and so more food for the detritivorous insects.

Hyde Clarendon Sixth Form College                                                                 13
(iii)   Most candidates realised that decomposers would respire compounds from the dead
                    plant remains, releasing carbon dioxide, which could be taken in by plants to be
                    used in photosynthesis.
                    However, only better candidates knew that the decomposers were saprobionts and
                    supplied details of extra-cellular digestion.




18.   Essays remain extremely variable in quality. For some candidates they provided the saving
      grace and did much to redeem the limited quality of the two structured questions. Others proved
      themselves unable to recall basic A-level knowledge, and produced superficial and poorly
      constructed accounts. The biggest single factor in limiting the marks awarded was undoubtedly
      the ability to base the essay on appropriate, detailed biology. Thus, for example, many of the
      essays on bacteria ignored the detail of genetic engineering, nutrient cycles and cellulose
      digestion which form a major part of the A-level specification and, instead, centred their essays
      around such topics as yoghurt and cheese production. Essays generally met the requirements for
      breadth although some devoted so much time to scene setting and significance that they left
      themselves little time to consider more fundamental issues. There is little doubt that plans would
      have helped here but these were often conspicuous by their absence. Given the pressure of
      writing under examination conditions, the quality of written communication was usually sound,
      often better than that displayed by the same candidates in Questions 1 and 2. The use of
      technical language, however, was less impressive and a lack of understanding of the terms “ion”
      and “bacteria” provided an obvious handicap.


      Certain topics lent themselves to excellent accounts of how inorganic ions are used
      physiologically - nerve impulses, nephron function, absorption in the intestine, root pressure and
      guard cell activity. These topics enabled candidates to demonstrate what they knew and how
      well they understood the processes concerned. There were many detailed and accurate accounts
      concerning the roles of sodium and potassium ions in the transmission of nerve impulses and of
      calcium ions in synaptic transmission. Less able candidates sometimes confused the roles of the
      ions or the parts played by diffusion and active transport. The movement of ions in the counter-
      current mechanism of the loop of Henle also appeared frequently, but here confusion over detail
      was more apparent.


      The identity of inorganic ions and their names produced problems for some in describing the
      role of ions in the synthesis of biologically important molecules. Phosphorus, for example, was
      commonly identified as the ion important in the synthesis of ATP and phospholipid. This lack of
      understanding occasionally led to much irrelevance, especially where discussing hydrogen
      bonding in DNA, or the importance of carbon, oxygen and hydrogen in macromolecules.
      The role of hydrogen ions gave scope for some sound biological detail in descriptions of the
      role of reduced coenzymes in photosynthesis and respiration. Few candidates considered the
      importance of hydrogen ions in changing the pH environment of enzymes, but many discussed
      the buffering effect of haemoglobin when describing the transport of carbon dioxide as
      hydrogencarbonate in the blood.




Hyde Clarendon Sixth Form College                                                             14
19.   Essays remain extremely variable in quality. For some candidates they provided the saving
      grace and did much to redeem the limited quality of the two structured questions. Others proved
      themselves unable to recall basic A-level knowledge, and produced superficial and poorly
      constructed accounts. The biggest single factor in limiting the marks awarded was undoubtedly
      the ability to base the essay on appropriate, detailed biology. Thus, for example, many of the
      essays on bacteria ignored the detail of genetic engineering, nutrient cycles and cellulose
      digestion which form a major part of the A-level specification and, instead, centred their essays
      around such topics as yoghurt and cheese production. Essays generally met the requirements for
      breadth although some devoted so much time to scene setting and significance that they left
      themselves little time to consider more fundamental issues. There is little doubt that plans would
      have helped here but these were often conspicuous by their absence. Given the pressure of
      writing under examination conditions, the quality of written communication was usually sound,
      often better than that displayed by the same candidates in Questions 1 and 2. The use of
      technical language, however, was less impressive and a lack of understanding of the terms “ion”
      and “bacteria” provided an obvious handicap.


      Although this option was frequently chosen by less able candidates, overall it was impressive to
      note that many identified the key areas of nutrient cycling, mutualistic relationships and the uses
      of bacteria in enzyme production and genetic engineering, and wrote about these topics with
      conviction.
      Better candidates demonstrated a good understanding of the role of bacteria in the nitrogen
      cycle although the origins of ammonia were often omitted from discussion. A number of
      frequent misconceptions studded the work of less able candidates; denitrifying bacteria were
      considered to play a key role in “restarting” the cycle, nitrifying bacteria commonly converted
      nitrates to ammonium compounds and nitrogen fixation involved the conversion of “nitrogen in
      lightning” to nitrates.


      There were many excellent accounts of the role of bacteria in ruminant digestion. The best of
      these not only considered cellulose hydrolysis but also discussed the production of fatty acids,
      and the conversion of ammonia to urea and its subsequent secretion in saliva. More limited
      candidates confined their answers largely to the bacterial digestion of cellulose. These accounts
      were characterised by superficiality rather than by error.
      Genetic engineering provided a third line of approach and, again, there were some very good
      accounts where candidates showed a secure understanding of basic principles. The less
      convincing accounts showed confusion over detail. Not infrequently, the role of restriction
      enzymes was held to be to remove sections of DNA from plasmids, and many considered the
      role of genetically engineered bacteria to be in producing many copies of the gene coding for
      insulin. In addition, some of the terminology was heavily reliant on the use of molecular “glue”
      and “scissors” than on the relevant enzymes. Some good accounts were subsequently spoilt by
      the introduction of poorly remembered examples from the food industry:
            yoghurt, cheese and, not infrequently, yeast in the production of alcohol.




Hyde Clarendon Sixth Form College                                                              15
20.   (a)   Too many candidates saw two empty boxes in the flowchart in (i) and either wrote the
            names of both substances in the boxes or the number of carbon atoms in each substance.
            This clearly is the result of not reading the question carefully. Those who did answer the
            question set, usually scored both marks. In part (ii) good candidates realised that all ATP
            is produced in mitochondria, except that produced in glycolysis. They therefore arrived at
            the correct answer of 36 ATP by deducting 2 from the net total yield of 38 ATP per
            molecule of glucose, or by deducting 4 from the total production of 40 ATP. Others did
            arrive at the correct answer by working out where each molecule of ATP was produced,
            but many attempting this method did so in a disorganised way and so made errors in
            calculation. In (iii) most candidates knew that, in the absence of oxygen, some of the
            reactions of respiration could not take place, but many were unable to describe the extent
            of anaerobic respiration. Well prepared candidates were able to state clearly that only
            glycolysis would take place and, therefore, the ATP production of the Krebs cycle and
            electron transport chain would be lost. They also often


      (b)   Despite being given specific information in part (i) concerning the features of the
            heterocysts (thick walls and the absence of chlorophyll), and the requirements of nitrogen
            fixation (anaerobic conditions) candidates too often invented other features and reasons
            other than maintaining anaerobic conditions for those features. Disappointingly few
            candidates confined themselves to answers based on excluding oxygen and not producing
            oxygen, which would inhibit the process of nitrogen fixation. There were some excellent
            answers to part (ii) from candidates who appreciated that nitrogen-containing compounds
            in the rice plants would be the starting point for the reactions of the nitrogen cycle, and
            duly described the roles of decomposition and nitrification accurately and logically. Some
            realised that the decomposers would produce carbon dioxide as a result of their
            respiration and that this could be used in photosynthesis by the leaves of the rice plants.
            However, too many just assumed that the ammonia produced by the heterocysts would be
            released into the soil, apparently unused by the fern and, in their answers, took this as the
            starting point for the nitrogen cycle. This clearly shows less appreciation of the situation
            as described.




21.   (a)   In (i), almost everybody sketched the pyramid correctly. Some did not draw the pyramid
            in the conventional way; in effect they drew it upside down, but still showed the correct
            relationships between the trophic levels. A few candidates failed to the gain the mark
            because they did not label their diagram. Likewise, in (ii), nearly everyone knew that
            energy is lost between trophic levels and could suggest at least one way in which it is lost.


      (b)   In this question, it was a relatively common failing for candidates to be unable to write
            about the relevant part of the nitrogen cycle without also trying to include other aspects.
            In (i), some candidates failed to distinguish between the roles of decomposers and
            nitrifying bacteria in their answers, phrasing their responses along the lines of “the
            decomposers and nitrifying bacteria convert the organic substances into ammonia and
            then to nitrates”. It was also disappointing to read the number of answers that included
            references to lightning and the Haber process. However, candidates who understood the
            nitrogen cycle well usually had little problem with this question. There was a general
            understanding in (ii) that nitrogen-fixing bacteria convert nitrogen gas into a form that is
            more readily available; however, there was also a widespread misconception that they
            convert the gas directly into nitrate ions.


      (c)   In (i), a disappointing number of candidates did not read the question carefully and
            described changes in the populations of both types of bacteria and both types of

Hyde Clarendon Sixth Form College                                                               16
protoctistans, usually without really explaining the reasons for any of the changes. Good
            candidates recognised the predator-prey relationship between the dispersed bacteria and
            the free-swimming protoctistans in the way the numbers increased and then declined
            slightly out of phase with each other. In (ii), candidates who understood the process of
            succession were generally able to recognise the changes in the environment in the
            treatment tank that resulted in changes in the community inhabiting that environment. A
            common failing was not to make clear that it is the activities of the organisms that inhabit
            an area that change the environment and so make it suitable for colonisation by other
            species.




22.   Unit 8
      It is difficult to comment meaningfully on the standard of the essays this year compared to that
      in previous years. Essays ranged from the outstanding to the extremely weak and their quality
      appeared at times to be independent of the quality of the responses to the other questions in the
      paper. Thus, there were some excellent answers to questions 1 and 2 which were followed by
      extremely limited essays and, at the other extreme, papers whose sole redeeming feature was a
      competent essay. Particularly apparent this year were the many essays which showed no
      evidence whatsoever of a plan. They were often poorly organised and lacked coherence, with
      timing often presenting an additional problem. Many candidates still see length in itself as a
      virtue. Such candidates often produced scripts with two or three extra sheets attached. They
      often started with some promise but frequently incorporated much that at best could only be
      regarded as being of marginal relevance.


      There were some outstanding essays on this topic where the detail throughout was fully in
      keeping with what might be expected of an A-level candidate, and which did not stray from the
      theme of the relationship between structure and function. Such essays were a pleasure to read
      and mark. Others, although sound and attracting considerable credit, tended to belabour
      particular aspects unnecessarily. A frequent example of this was in protein structure where the
      concept of shape and fit was illustrated at considerable length with enzymes, hormones, carriers,
      and antibodies. All were correct and each was relevant but, taken together, they contributed little
      more than could be provided by considering one example. This approach not infrequently led to
      candidates discussing one particular polymer at great length at the expense of others, thus
      affecting the overall balance of the essay. At the other end of the spectrum, it was apparent that
      some candidates were uncertain as to the meaning of the term ‘polymer’. It was not unusual to
      see substantial paragraphs written about lipids, and there were also frequent references to “small
      polymers such as glucose and maltose”. Apart from this, errors often arose from a poor
      understanding of technical terminology. Thus a- and P-glucose were regularly confused;
      cellulose molecules were described as consisting of p-pleated sheets, and there was considerable
      uncertainty over the relationship between amino acids and DNA, the latter all too frequently
      being described as a polymer of the former.




Hyde Clarendon Sixth Form College                                                              17
Unit 9
      The essays varied greatly in length and biological content, but less so in breadth and relevance.
      Many essays showed good evidence of planning in a way which would clearly be useful to the
      author, and which kept their work relevant. Some candidates failed to plan at all.
      The competence with which the scientific terminology was used was pleasing, with many
      technical terms being used in the majority of essays. Weaker candidates tended to relate their
      work in everyday language, dwelling on the distribution of large surface areas rather than on the
      link with transfer processes.


      Many of the candidates who chose to write this essay showed themselves to be competent
      biochemists. They understood the nature of a polymer, and frequently opened their essay with a
      definition. Some of the best candidates were at pains to point out that lipids were not polymers,
      and so would not be discussing them. Sadly, some thought they were, and included as much on
      this group of substances as they did on the relevant ones.


      The most frequently discussed polymers were proteins and carbohydrates. Extensive and
      detailed knowledge of the structure of proteins was included, with descriptions of peptide
      bonds, primary to quaternary structures, and the bonds which hold them together. Weaker
      candidates were somewhat haphazard in their descriptions, but the better ones were not only
      capable of demonstrating a lot of detail but their approach was highly logical. The links with
      function were less competent. Some simply offered a selection of proteins, while the best essays
      discussed the differences, for example, between those with a simple secondary structure
      compared with the globular examples. Frequent choices were enzymes and haemoglobin. Other
      interesting additions included keratin, antibodies and hormones; although most felt there was no
      need to comment on the fact that not all hormones are proteins. As a consequence a few lipids
      were mentioned. The work on the structure was in far more detail than the function in many
      cases, leaving candidates missing the opportunity to demonstrate detailed knowledge of the
      control of blood glucose, the principles of immunology, and the transport of blood gases. This
      was a route which could have been exploited by those capable biologists whose biochemistry is
      perhaps a little sketchy.


      The work on the structure of carbohydrates was equally detailed by a lot of candidates who
      chose this essay. However, it was much more often that the function of the molecule was linked
      very closely to its structure. Starch and glycogen were described as large, compact molecules
      while the work on cellulose reflected its role in the structure of a plant. There was some
      confusion over the solubility of some of these molecules.


      Candidates who moved on to discuss the nucleic acids were less frequently seen. Those that did
      gave good descriptions of their structure, but a number went on to give huge amounts of detail
      about the processes in which they play a part, rather than making the more careful links to their
      function. Full details of protein synthesis, DNA replication, and genetic engineering, were
      inappropriate. Weaker candidates confused nucleic acids with proteins.




Hyde Clarendon Sixth Form College                                                              18
It was easy for candidates to use this essay to show their A-level knowledge. Some candidates
      tried to use the outline of an essay they may have written earlier on a single polymer. There was
      a wide range of marks, with a good number of the better candidates gaining full or close to full
      marks.




23.   Part (a)(i) appeared to indicate a problem over the term ‘organic’, as the most common incorrect
      answers were ammonia and nitrate. In part (ii) the process of death or decay was often given
      rather than a source of the material. Nitrifying, nitrogen fixing and denitrifying bacteria caused
      confusion in answers to part (b); a substantial number of candidates did not know which type of
      bacteria did what. Although this flow diagram was not the usual form of the nitrogen cycle,
      enough clues were given to allow candidates to relate it to their own knowledge, and many were
      able to do so. 183 was a common incorrect answer to part (c), obtained by deducting the value
      for ‘cattle’ from that for inorganic nitrogen’. Many gave 339 which suggested that they had the
      principle correct, but missed out the effect of fixation in providing some of the ‘nitrogen’
      requirements of clover. While a lot of the candidates knew that clover had nitrogen fixing
      bacteria in root nodules, a significant number thought that the clover did its own nitrogen, fixing
      in part (d). A common circular argument was that cattle eat the clover, add the nitrogen from the
      clover to the soil via dung and urine to benefit the grass which is an advantage to the cattle.
      Only a few suggested that growing clover might save the farmer having to buy and apply
      fertiliser.




24.   ‘Ammonia’ rather than ammonium was a common response in part (b). Although many
      candidates made correct references to nitrifying bacteria in part (b), few were unable to give
      even a GCSE level response to the processes involved in the production of ammonium ions.




25.   Three times as many candidates attempted the essay on cycles in biology as on the structure and
      function of carbohydrates. As expected, the carbohydrates essay tended to be shorter but there
      was no evidence to suggest that this title attracted less able candidates. Most candidates
      provided some form of plan, although these varied greatly in length and detail. Fortunately,
      compared to last year, fewer candidates spent a disproportionate amount of their time on a plan
      to the detriment of the essay itself. There was some evidence to support the view that candidates
      were better prepared for the essays, particularly in terms of providing relevant information and
      breadth of knowledge. Most examiners commented on a general improvement from last year
      although quality of written communication and poor handwriting remains a problem for a
      significant number of candidates.


      This essay title enabled candidates to include a wide range of topics from several modules. As
      with essay (a), content beyond the specification, and to a greater extent from the option
      modules, was also credited. Almost invariably candidates included the carbon and nitrogen
      cycles in their answers. The weakest candidates limited their discussions to superficial accounts
      of these nutrient cycles and often the water cycle, with few if any factual details of A level
      standard. However, most candidates were able to provide a greater breadth of knowledge and
      included metabolic, physiological and in some cases life cycles in their essays.




Hyde Clarendon Sixth Form College                                                              19
The quality of written communication in relation to nutrient cycles was particularly
      disappointing. Many candidates referred to animals obtaining ‘nitrogen’ from plants, and
      decomposers releasing ‘carbon’ and ‘nitrogen’. Plants ‘breathing in gases’ and energy being
      ‘produced’ or ‘recycled’ were not uncommon phrases. Many candidates suggested that nitrifying
      bacteria are involved in nitrogen fixation. There was also some confusion over the role of
      decomposers with few candidates specifying the nitrogen compounds involved. Many
      candidates did not display an understanding of nutrient cycles, with some suggesting that the
      importance of the nitrogen cycle is to maintain the nitrogen content in the air.


      Most candidates mentioned the Krebs cycle, with many candidates also referring to the light-
      independent reaction. Descriptions of the Krebs cycle often failed to complete the recycling of
      the four-carbon compound and tended to concentrate on ATP production. Unfortunately, very
      few candidates described the cyclical features of the electron transport chain. Similarly, the
      cyclic nature of ATP formation and breakdown was rarely considered. Nevertheless, there were
      some excellent accounts of the Krebs cycle which outlined the importance of regenerating a
      four-carbon compound to react with acetyl coenzyme A. Generally candidates displayed a
      clearer understanding of the regeneration of RuBP in the light-independent reaction. However,
      there was still the tendency for candidates to provide incomplete cycles.
      Discussion of metabolic cycles often led to irrelevancies, particularly glycolysis and non-cyclic
      features of the light-dependent reaction.


      Where attempted, candidates provided some worthwhile attempts to convey the cyclical nature
      of negative feedback, usually in relation to the control of blood glucose. However, a significant
      number of candidates referred to insulin and glucagon as enzymes and there was some
      confusion between glycogen and glucagon. Many candidates provided details of the ornithine
      cycle even though this is not required in this specification. A significant number of candidates,
      presumably those taking option 8, included the menstrual cycle in their essays. However, many
      of these candidates included irrelevant information such as details of the process of fertilization.
      References to synaptic transmission and actomyosin bridge formation as cyclical processes were
      infrequent. However, when attempted, these descriptions were detailed and comprehensive. This
      was certainly not the case for descriptions of the cardiac cycle. These accounts were often
      confused and failed to relate pressure and volume changes in the heart and aorta to events in the
      cardiac cycle.


      A significant number of candidates included descriptions of mitosis and meiosis, often towards
      the end of the essay. These descriptions were often superficial or contained numerous
      inaccuracies. Candidates were often confused between chromatids and chromosomes and many
      had difficulty explaining the significance of meiosis in a life cycle. A few candidates provided
      detailed descriptions of predator / prey cycles or viral replication.




26.   (a)   In general, this question was not well answered in that too many failed to realise that it
            was a three-mark question and just gave one idea, rephrased or extended. Surprisingly
            few candidates recognised that monoculture provides a huge food supply and hence
            promotes rapid population increase within a pest population. Most recognised that the
            pest could have an increased effect on the crop in a monoculture.




Hyde Clarendon Sixth Form College                                                               20
(b)    (i)    The majority of candidates were able to identify that the ‘resistance’ allele is
                    recessive, that the parents would therefore be heterozygous, and that the resistant
                    offspring would be homozygous recessive. Too many incorrectly referred to
                    mutations resulting in resistance.
             (ii)   Most candidates scored well with bioaccumulation and killing non-target
                    organisms being the most frequently seen correct responses. It was surprising to
                    see a significant number of candidates referring to the pesticide leaching into the
                    water and causing eutrophication.




27.   (i)    Most candidates gained credit for the fact that the fertiliser had leached into the river, but
             only the more able explained that it is excess fertiliser that leaches in this way.
      (ii)   The vast majority of candidates scored highly by correctly recounting the standard
             sequence of events in eutrophication.



28. No Report available for this question.




Hyde Clarendon Sixth Form College                                                                 21

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Tropical homestead system uses bacterial fermentation and nitrogen cycle

  • 1. 1. A diagram was provided that showed part of a system designed to meet all basic needs of a family of four living in the tropics. The system was based in part on the generation of fuel gas by bacterial fermentation of urine and faeces. (a) Candidates were asked to describe how processes which naturally formed part of the nitrogen cycle could make nitrogen contained in urine and faeces available to crop plants. There were many competent answers that showed detailed knowledge of the relevant parts of the nitrogen cycle and scored full marks. There were also very weak accounts, however, that included unselective descriptions of the whole of the nitrogen cycle including inappropriate references to denitrification and nitrogen fixation. The average type of response tended to include one or other of the following faults: the assertion that ammonia was present in urine or faeces; use of the term “nitrogen” in a very loose way with little reference to specific forms; errors in the sequence of nitrite and nitrate production; confusion in the nomenclature of the microorganisms involved; lack of precision concerning uptake of nitrate by plants. (b) In the system, solid waste from the fermenter was used as fertiliser for crop plants and candidates were asked to explain the advantage of growing leguminous plants such as groundnuts or beans. It was evident that many candidates were unfamiliar with leguminous plants. Answers were often quite weak, with scoring limited to mention of root nodules (root “noodles” were held to be nutritious, but unacceptable here) and nitrogen fixing bacteria (with the latter frequently misidentified). There was widespread belief that nitrate was the product of nitrogen fixation and, in some cases, that the plant itself fixed the nitrogen. Few candidates appreciated that remains of the legumes would need to be decomposed before releasing material that could be converted to nitrates and consequently there were many vague answers such as “legumes return goodness to the soil”. Ironically, some of those who had discussed nitrogen fixation entirely out of context in part (a) did not repeat this material which was now relevant. The question concluded by asking candidates to explain the advantage in the system of stocking the pond with fish that feed on algae rather than with carnivorous fish. Most candidates chose to refer, sometimes at undue length, to prevention of eutrophication. Very few, however, discussed trophic levels in relation to relative energy loss but some did suggest that stocking with fish that feed on algae would produce a greater yield of fish. A large number of obscure answers suggested that carnivorous fish would need to be fed by “bringing meat in” and there was even the occasional idea that the cattle and chickens would be fed to these fish. 2. There was a great deal of confusion between nitrogen fixing and denitrifying bacteria in part (a). Candidates are still not clear about the sequence of changes in the nitrogen cycle. Some failed to read the question carefully and began with nitrogen gas in the atmosphere rather than nitrogen compounds in dead spruce seeds. Weaker candidates still fail to define the compound containing the atom of nitrogen. Hyde Clarendon Sixth Form College 1
  • 2. Decomposition or ammonification releasing ammonia from organic sources of nitrogen still seems to be the weak link. Candidates frequently misread part (b), and often suggested that moss must contain nitrogen fixing bacteria in their roots. Many spotted that competition was involved but were unsure of the factor for which the organisms were competing. Again, competing for nitrogen was a common error. 3. The essays indicated that most candidates had given thought to organising material into a logical and coherent account. The Quality of Language was considerably better and there was substantially less of the colloquial expression that marred many essays in previous years. The main criticism in this area was the widespread failure to present new ideas in discrete paragraphs. However, as was the case last year, the scientific content of the essays proved to be disappointing. Work was often extremely superficial and not infrequently failed to reflect the detail expected of candidates following an A Level course of study. Relationships between animals and plants This was the least popular of the essays and was often chosen by the weaker candidates. As a result, many responses offered no more than a brief discussion of animal dependence on food and oxygen from plant photosynthesis before embarking on lengthy and often anecdotal accounts of the provision of habitats, shelter and building materials. Only rarely did such accounts offer an appropriately detailed discussion of trophic levels, energy transfer and carbon dioxide/oxygen balance. Where nutrient cycling was introduced, the better candidates successfully established the link between animal excretion, decomposition and the uptake of mineral ions by plants. Many less able candidates lost sight of the question at this point and essays digressed into discussions of the roles of various micro-organisms. Other areas which led to the introduction of much peripheral matter were references to pharmaceutical compounds derived from plants and to habitat destruction. Some accounts were totally irrelevant and suggested that candidates had misinterpreted the question as requiring a discussion of the similarities and differences between animals and plants. 4. In part (a) it was obvious that some candidates had actually done this sort of exercise as they described in detail how random numbers are generated and used with a grid on the ground to place the quadrats. A minority of students still described throwing random quadrats but this method is prone to bias and is not random. The most common error was to omit the method of generating random numbers. Many wrote at length in part (b)without focusing on the stages of heather growth given in the diagram, or failed even to offer an indication of the heather’s age, and vaguely used the terms ‘early’ and ‘late’. The diversity of heather and not of the whole community was a popular misunderstanding. Some candidates agreed that the diversity would change but failed to say whether it would get greater or less. Although many did use the information in the table to explain that increased cover offered by the heather, blocked light and affected diversity. Many offered other factors such as more nutrients in the soil, but could not easily rationalise how that occurred. Hyde Clarendon Sixth Form College 2
  • 3. In part (c)(i) some candidates did manage to calculate the rate but this caused problems for many. Some demonstrated misunderstandings with the correct notation of SI units. Few read the question in part (c)(ii)so many did not relate age of the heather to the changing distribution of green shoots and older woody ones. The most common incorrect response involved a discussion of the death of parts of the plant. In part (iii) the availability of extra food for grouse was frequently given, but few related the fact that burning heather eventually increased cover, which provided the grouse with somewhere to hide. In part (d)(i) many students failed to show an understanding of the term ‘organic’. This can be the only explanation for responses such as ammonia, soil, humus, bone and root. Some however did offer protein and few even gave DNA. Candidates really had a problem with (d)(ii) as phosphate, sodium, and hydrogen ions were given, this suggested that the candidates read the question as “name an ion” rather than “name an ion containing nitrogen”. Ammonia and the correct named ion, but with an incorrect chemical formula, were common mistakes. In part (e) there was still a great deal of confusion over the role of the bacteria and the sequence of the nitrogen-containing compounds. The relative positions of ammonium, nitrite and nitrate ions were regularly switched. Many candidates did, however, correctly name the bacteria involved in the nitrogen cycle and gave complete and accurate accounts. Saprobiotic bacteria were rarely mentioned and often the pathway began with the ammonium ions, but with no mention as to how they were created from organic nitrogenous compounds. Inappropriate bacteria such as the nitrogen-fixing bacteria were often mentioned, candidates being unable to identify the relevant parts of the nitrogen cycle and so found it necessary to include everything they knew. In part (f) most candidates realised that the table showed changing amounts of nitrogen in the soil and heather plants, but they were at a loss to explain the reason for burning heather after 8 rather than after 15 years. Few realised the significance of creating gaseous oxides of nitrogen during burning and thought that burning would place nitrogen directly into the soil. 5. (a) There was a strong centre bias to this part of the question. Candidates were either familiar with the ways in which organisms were classified or their answers appeared t6 be based largely on guesses. (b) In part (i) most candidates appeared to be of the opinion that digestive enzymes were present in worm casts so that digestion could continue in the soil. Some were of the opinion that, as worms were primitive animals, they had short guts. They therefore ingested the casts and passed them through the gut a second time. There were relatively few references to the key points that the enzymes were not themselves digested and being proteins they were too large to be absorbed through the gut wall. Some of the answers to part (ii)showed a good understanding of the basic principles of experimental design, but far too many simply assumed that demonstration of the presence of reducing sugar or the absence of starch in worm casts proved the presence of amylase. Hyde Clarendon Sixth Form College 3
  • 4. (c) Many candidates were content to take the lack of earthworms at face value and made no attempt to link it in any sensible way to the use of fungicide. Some realised that the information in the passage about breaking the leaves into smaller pieces was relevant, but few went on to develop this point further and describe the effect of increased surface area on the rate of microbial decomposition. (d) The answers to this part of the question were generally poor. Although there were some references in part (i) to nitrate and ammonium ions, mineralised nitrogen was rarely described as being more than “nitrogen in mineral form”. The tendency to repeat the question also characterised many of the answers to part (ii) with excretory nitrogen generally described as being “nitrogen in excreted products”. Where the answer was amplified, excretory nitrogen was usually identified as the nitrogen found in faeces. Evidence from the answers to this part of the question, and elsewhere in the paper, point to few candidates understanding the differences between the processes of secretion, excretion and egestion. (e) The number of times that ammonia appeared in the answer to part (i) suggested that either few candidates understood the meaning of the word “organic” or that ammonia was genuinely regarded as an example of an organic compound. Where both of the examples offered were organic, lipids and carbohydrates often featured. There were, in addition, many inappropriate references to cells and tissues. Although there were some excellent answers to part (ii), others reflected a fundamental lack of knowledge on the part of the candidates concerned or were totally unselective in the material they presented. The production of ammonia was often omitted, the terms “nitrifying” and “nitrogen-fixing” were frequently confused and nitrates were too often described as being converted into nitrites. (f) In part (i) most candidates showed understanding that the behaviour of earthworms in dry conditions led to a smaller surface area over which water could be lost. Some, unfortunately, linked this behaviour to saving energy through a lower rate of respiration, while others referred to reduced rates of sweating. There were also many sound answers to part (ii). (g) Many candidates understood the difficult concept that lowering the C : N ratio could be achieved either by removing carbon or by adding nitrogen. Unfortunately, the explanations of how these effects were achieved Were often insufficiently clear to gain credit. Once again, far too much use was made of unqualified pronouns and many members of the examining team commented on difficulties in deciding to what “it” was referring. 6. Many candidates did not study the diagram of energy flow through the ecosystem with sufficient care. (a) This was concerned with the possible fates of energy already absorbed by the producers but not yet transferred to organic molecules. Hence reference to energy loss by reflection was irrelevant, and respiratory loss was inappropriate, especially as the diagram showed this at a later stage. Hyde Clarendon Sixth Form College 4
  • 5. (b) In calculating the energy transfer to the primary consumers, many candidates failed to subtract the value for material in the producers which was not actually consumed, hence 8760 was a more common answer than the correct 2920 kJ m –2. (c) Many candidates displayed a sound grasp of the topic of nitrification by soil bacteria. Some spoiled their answers by giving supplementary, incorrect chemical formulae (e.g. “nitrate / NH3”). Some candidates included irrelevant extra material about nitrogen fixation and denitrification, and some had these different aspects of the nitrogen cycle totally confused. 7. This question required the use of extended prose throughout. Full marks were only available to those able to express the appropriate concepts clearly and unambiguously in scientific terms. Many candidates produced good answers, using both information from the passage and their own biological knowledge. Lack of detail, misconceptions and careless expression all contributed to the loss of marks for other candidates. (a) The main points given by candidates here were related to deforestation causing loss of habitat, loss of food, and potential extinction or the need to migrate elsewhere. Some gave details relating to exposure of the soil and hence erosion and leaching of ions. Very few mentioned changes in climate or in light levels as a result of removal of the trees. (b) Many understood that trees could act as carbon sinks by absorbing carbon dioxide (but not ‘carbon’ as a high proportion of weak candidates stated) for use in photosynthesis and conversion of organic molecules within the tree. Careless omission of any of these details resulted in the loss of marks – the third point being the one most commonly left out. Far too many candidates believed that carbon dioxide was converted into oxygen or that it was used in ‘respiration’ rather than photosynthesis. (c) Some candidates did not make it clear whether the scenario they were describing related to the situation where ploughing took place or, as the question stated, to where ploughing was reduced. Such ambiguity in the answer cost marks. Once more, ‘carbon’ was often the substance used rather than dead organic matter and this carbon was often thought to combine directly with oxygen to form carbon dioxide. Ploughing sometimes introduced ‘air’ into the soil, with oxygen not being specified. Similarly, the process of respiration, if mentioned in the context of saprotrophic organisms in the soil, was rarely qualified as being aerobic. Some candidates even imagined a peculiar situation whereby carbon dioxide was still formed in the soil but it was trapped due to a lack of ploughing. Hyde Clarendon Sixth Form College 5
  • 6. (d) This section was specifically about the role of nitrifying bacteria. These were frequently confused with saprotrophic and with nitrogen fixing bacteria. For those thinking the latter, ploughing introduced nitrogen gas into the soil ready for fixation. Only correct, relevant details were rewarded, such as the conversion of ammonium ions to nitrite and then to nitrate, using the extra oxygen introduced by ploughing. Many candidates knew that nitrate ions were taken up by crop plants and some explained how these could be converted into named organic compounds, such as DNA or protein, which could be used in turn by the plant to increase its growth or yield (hence being of benefit to the crop plants, as required by the question). Such completeness of detail was the domain of the better candidates who frequently scored full marks. 8. (a) Weak answers followed the stem of the question and only referred to carbon rather than considering the form it would take in the atmosphere, namely carbon dioxide. Unclear responses proposed that ‘slash and burn’ would increase its concentration without explaining whether it was the ‘slash’, the removal of trees, or ‘burn’, the combustion of trees, that was responsible for the change. Not all candidates used the allocation of marks to construct their answer and generally included only the increase due to release of carbon dioxide from burning. Better candidates identified the reduced uptake of carbon dioxide since there would be fewer photosynthesising organisms. (b) The concept tested in this question was secondary succession. Where the role of pioneer species was identified, clarification was needed since soil already existed from the earlier presence of organisms. Weak responses produced unnecessary descriptions of primary succession. Better candidates recognised that recolonisation would occur with seeds and spores from neighbouring areas although this was not always well expressed. Many achieved credit by explaining the process of succession and the resultant re-establishment of a climax community, but there were also many who appeared unfamiliar with the concept or who could not communicate the main ideas adequately. (c) This question allowed candidates to demonstrate their ability to interpret information and apply their knowledge. Thus, good candidates could identify the source of ammonium compounds in manure, which soil bacteria would be relevant, and what was meant by crop yield. Many showed a good knowledge of the nitrogen cycle but only the better candidates considered the role of nitrogen-fixing bacteria. It was not necessary to name a specific nitrifying bacterium, but where this was done, the bacterium had to be linked to the appropriate reaction to secure credit. (d) It was pleasing to see so many candidates familiar with environmental concerns and conservation, although candidates often failed to express themselves clearly. A large proportion considered the view of what would be lacking if a forest ecosystem was removed, rather than the advantages of conserving one, but examiners sought to credit the concepts. There was a limited appreciation of forests as sustainable resources but frequent reference to habitats, carbon sinks, species diversity and food chains allowed maximum credit to be obtained by many. Hyde Clarendon Sixth Form College 6
  • 7. 9. (a) Some candidates were able to gain maximum credit by linking contraction of the diaphragm and intercostal muscles to an increase in volume of the chest and a decrease in pressure, but there were many who gained little credit for their responses. Of these, some failed to take note of the requirements of the question and emphasised the role of chemoreceptors, some clearly confused inspiration with expiration, and others wrote in extremely simplistic terms about unspecified muscles “working” and the chest “getting bigger”. (b) Candidates who identified part (i) as a question relating to experimental technique produced sound answers, commenting on the need to allow breathing rate and tidal volume to stabilise. Others responded inappropriately with such answers as “allowing the average to be worked out” or “warming up”. The mathematical problem in part (ii) created relatively few difficulties, although there were those who were uncertain of the required approach, or incorrectly converted the figures to cm3. The examiners had little sympathy with those candidates who confessed at this point that they did not have calculators. Answers to part (iii) were almost universally disappointing. Although candidates clearly experienced little difficulty with the wording of the question, they did not take sufficient care in picking out the underlying trends, a feature which also frequently marks their approach to coursework. Thus many did no more than describe an “increase in breathing rate and tidal volume”. Good answers needed to refer to the change in gradient. 10. Only the better candidates were able to give satisfactory answers to the sections requiring understanding of the chemical aspects of the process of photosynthesis. (a) Many were confused concerning the identity of the carbon dioxide acceptor substance in the Calvin cycle. Not many of those that did know this to be RuBP went on to stress that two molecules of GP would be formed per molecule of carbon dioxide fixed. The concept of equilibrium existing between the rates of formation and breakdown of GP was foreign to all but the best candidates. Many were beguiled by the superficial resemblance of the relevant part of the graph to a typical ‘limiting factors’ curve. Some others argued in favour of some sort of saturation phenomenon. (b) In order to explain that the concentration of GP was increasing in the dark, many candidates concentrated, incorrectly, on the formation of GP rather than on its lack of conversion to other substances. Some even invoked GP production in glycolysis (forgetting that it would also have to be broken down before any ATP were produced). Only the most able candidates realised that conversion of GP to TP required ATP and reduced NADP from the lightdependent reactions and that these would, of course, soon be depleted in the dark. Hyde Clarendon Sixth Form College 7
  • 8. (c) Most candidates tended to be successful in their interpretation of the data in the table and understood that, in the light (between 8 am and 4 pm) photosynthesis would occur, hence removing carbon dioxide from the air and leaving a lower concentration, as shown in the data. Many also appreciated that the absence of leaves, or reduced light levels, or even the production of carbon dioxide by soil organisms, would result in a higher concentration of carbon dioxide at ground level than that found higher up among the leaves of the plants. (d) Many candidates realised that the wind could alter the concentration of carbon dioxide in the air, but not all of these explained that this would be due to the wind bringing in fresh supplies or, alternatively, removing carbon dioxide. Very few candidates went on to explain that the wind would therefore have introduced another experimental variable and hence made any measurements of carbon dioxide concentration in the air (which was the basis of the experiment) unreliable. There was also much irrelevant discussion of the effect of the wind on the rate of transpiration. (e) While many candidates appreciated that saprotrophic microorganisms would cause dead leaves to decay and hence release carbon dioxide which could then be taken up by the plants, fewer gave more specific details relating to the involvement of digestion and respiration. Many thought that ‘carbon’ would be released into soil for uptake by plant roots. Some could not resist the temptation to include irrelevant comments about the nitrogen cycle, despite carbon having been stated quite explicitly as the subject of the question. 11. This question required the use of extended prose throughout. Full marks were only available to those able to express the appropriate concepts clearly and unambiguously in scientific terms. Many candidates produced good answers. Lack of detail, misconceptions and careless expression all contributed to the loss of marks for other candidates. (a) Many candidates were very careless and gave few practical details of how sticky traps could be used to compare the diversity of flying insects in the two environments. Elementary procedures such as the use of multiple traps and details of how these would be placed at random were frequently overlooked. Most suggested that the number of individuals of each species caught by the traps should be counted and used to calculate an index of diversity, but many others were confused and thought that the mark-release- recapture technique should have been used in order to find the Lincoln Index. While most candidates appreciated that the diversity of insects in the forest would be greater than that in a cultivated field, some gave very little detail in their explanation of the reason for this. Better candidates included details regarding the relative harshness of the two environments (often giving the use of pesticides in cultivation as a factor detrimental to insects), and the abundance of different habitats and greater variety and quantity of food available in the forest. Many wasted both time and space by reiterating the converse argument for the second habitat rather than expressing themselves in comparative terms once only. (b) The explanation offered for why crops could only be grown for a few years in a field produced by clearing part of the forest were frequently vague. References were made to ‘nitrogen’ ‘running out’ with no detail such as the removal of material in the crops at Hyde Clarendon Sixth Form College 8
  • 9. harvest leaving little organic matter to decay in the soil, or to leaching of soluble nitrogen-containing ions such as ammonium and nitrate. The better candidates had little problem however in applying their knowledge and understanding of the nitrogen cycle to explain how the concentration of nitrates in the soil could be restored with time. Some mentioned nitrogen fixation (both atmospheric and biological) and most gave detailed accounts of putrefaction and nitrification. Weaker candidates tended to concentrate on colonisation and succession and lost sight of any connection with nitrate production. 12. BYA8 Candidates gave the impression of leaving themselves adequate time for attempting the essay and it was encouraging to note that many were able to write three or more sides on their chosen topic. Some obviously encountered considerable problems in deciding which of the two essays to attempt and it was not uncommon to see comprehensive plans relating to both and sometimes, even, a substantial false start. It is important, however, for candidates to appreciate that length in itself is not a merit. Care has to be taken over ensuring that the essay is written clearly enough for it to be read by the examiner. This was by no means always the case. There was surprisingly little evidence of planning in the work from some centres, and it is possible that this was the main reason for so many candidates involving themselves in so much needless repetition. Many candidates felt the need for a lengthy introduction defining the title. Unfortunately such introductions were frequently devoid of significant factual content or merely served to indicate that the candidate had little intention of restricting the essay to relevant processes. The carbon cycle was generally well understood in principle but accounts frequently extended over one or more pages and lacked significant detail, merely referring to carbon being transferred. Candidates who had a thorough knowledge of the nitrogen cycle often produced excellent accounts of this topic. Others experienced considerable problems. These often stemmed from failing to appreciate the underlying principles. Thus it was not uncommon to encounter candidates who saw the whole process as a mechanism centred on maintaining the atmospheric concentration of nitrogen constant. Such candidates inevitably started their accounts with a reference to nitrogen fixation and ended with denitrification. There was also much confusion between the events of the different stages in the cycle. Biochemical cycles featured in many accounts and, in general, the level of factual knowledge here was impressive. Unfortunately many saw inclusion of this material as an opportunity to display a broader understanding of the processes of respiration and photosynthesis. As a result, they included much material which could not be considered as relevant to an essay on cycles. Hyde Clarendon Sixth Form College 9
  • 10. The oestrous cycle was the best described physiological cycle and, where candidates had paced themselves sensibly and allowed time to incorporate detail of hormonal control, produced some excellent detail. Other cycles were less convincing and there were many accounts of physiological process such as synaptic transmission and digestion which, with the best will in the world, could not be considered as cyclical. A case could be made for the inclusion of other physiological processes such as those involving homeostasis, the circulation of blood and the mechanism controlling breathing, but these processes were also usually described as linear sequences or they lacked the necessary detail to add to the quality of the essay. BYA9 The essays varied greatly in length, depth of knowledge and relevance. The most common failing was the inclusion of a number of cycle names without at least a paragraph about the cycle at the appropriate depth. Candidates should not hesitate to use small, simple sketches (e.g. carbon cycle, structure of glucose) to illustrate their work, provided such diagrams are labelled accurately. The full range of marks was used on both essays, with Essay A being less popular than Essay B, and there was pleasing evidence of planning, both in the form of a plan and as demonstrated by a piece of coherent, flowing prose. There is a requirement for technical language at this level so terms such as ‘kicks in’, and ‘Mother Nature’ devalued the essays in which they appeared. It is important that candidates realise that erroneous or unsupported mention of an aspect of the specification may actually detract from the impression their essay gives. Candidates found it hard to introduce this topic succinctly. The expectation was that candidates would select at least one each from a range of cycles from which they could emphasise the common feature of the continuity of a cyclical process, describing the details in sufficient depth and in technical language such that they gained maximum marks. Some candidates achieved this ideal. The range covered included ecological cycles (usually nitrogen and carbon cycles), physiological cycles (the menstrual cycle, the cardiac cycle), biochemical cycles (the Krebs and Calvin cycles) and parasitic life cycles (Plasmodium and Schistosoma). Excellent candidates described the carbon and, more particularly, the nitrogen cycle in considerable depth. The range of ways in which plants could obtain the nitrogen-containing ions they need for protein synthesis was thoroughly explained, sometimes with faultless use of the names of the microorganisms involved. Weaker candidates muddled the process involved, confusing names and introducing erroneous ideas (‘carbon is taken up through the roots’, ‘plants take in carbon dioxide for respiration’, ‘when plants and trees are eaten, the nitrate they contain…’, etc.). In some cases, cycles were named but nothing was offered to explain them at all. Again some excellent accounts of the menstrual cycle were seen, including considerable detail on hormones and on uterine changes, The cardiac cycle was well described by a number of candidates, but many used this term to mean the circulation of blood from the heart to lungs and back again. These answers generally offered little detail or accuracy above average GCSE level. Hyde Clarendon Sixth Form College 10
  • 11. The Krebs and Calvin cycles were clearly set in context and briefly related to linked reactions by the better candidates. Weaker essays muddled the two processes or included lengthy (and irrelevant) sections on the ETC or the light-dependent reaction. It was, of course, possible to use cyclic photophosphorylation as a valid example of a cycle but few managed to do this successfully. Descriptions of parasitic life cycles were of variable quality. Some near perfect accounts were seen, but in others, particularly when describing schistosomiasis, the stages of the life cycle appeared to be a mystery. 13. Many candidates found this to be quite a difficult question. Answers were frequently expressed with insufficient clarity and data were often used non-selectively. (a) Most candidates realised that the data for the grass in graphs C and D showed no significant difference. Weaker candidates imagined that some of the slight differences were of significance. (b) For those who selected the appropriate graphs for comparison (i.e. A and C, with neither having Rhizobium added and only C having the nitrate), there was little difficulty in finding evidence for the effect of nitrate fertiliser on the soybeans. Less selective candidates produced very confused arguments. (c) This section required candidates to both describe and explain the effect of Rhizobium on the growth of the soybeans. Many candidates did either one or the other. Explanations were frequently confused regarding the details of nitrogen fixation and answers often included nitrate production in addition to, or instead of, that of ammonium ions. A sizeable minority realised that a mutualistic relationship existed between the Rhizobium and the legume (or that, at least, the bacterium formed nodules on the plant’s roots), and some went on to explain that the plant would gain organic nitrogen compounds such as amino acids or proteins as a result of the relationship. In sections (b) and (c), a sizeable proportion of candidates appeared confused regarding what had been used in the experiment as a measure of plant growth – there were many references to the ‘number of seeds’ produced but, since this was plotted on the x-axis, it should have been realised that the number of seeds was a manipulated variable and not a result. Hyde Clarendon Sixth Form College 11
  • 12. 14. (a) Some accounts of the activities of decomposers and nitrifying bacteria in the recycling of carbon and nitrogen from fallen leaves were truly excellent. Aspects which tended to be omitted were mainly those associated with the early stages of saprotrophic nutrition, i.e. the secretion of hydrolytic enzymes for external digestion followed by absorption of the products by diffusion or active transport. Details of respiration releasing carbon dioxide to be used by the trees in photosynthesis, of the release of ammonium compounds its conversion to nitrite and nitrate and the subsequent use of the latter by the trees to produce organic nitrogen compounds such as amino acids were frequently given. However, weaker candidates typically knew few of these details and often described how ‘carbon’ was released into the soil, in which state it was apparently taken up by the trees’ roots. Some candidates were determined to display all they knew about the nitrogen cycle and included irrelevant details of denitrification and nitrogen fixation. (b) For candidates who kept to the point, this was a very straightforward question: felled trees no longer removed carbon dioxide from the atmosphere by photosynthesis and burning them released more carbon dioxide back into the atmosphere – hence a predicted rise in the carbon dioxide concentration. Some became too involved in telling a story and often forgot about one or other of the key processes. (c) Answers to this section were very disappointing. Many candidates merely repeated information given in the stem of the question but did not use this, nor did they attempt to apply biological principles to the situation with which they were presented. Others treated examiners to the answer they had prepared earlier, with sound biological facts irrelevant to this question. One area which was hardly ever explored was how the protective canopy of the softwood trees might actually have modified the environment to make it suitable for the growth of hardwoods beneath it – such aspects as protection from wind and rainstorms, provision of a humid environment, shedding their leaves making ions available to the hardwoods, reducing soil erosion and leaching of ions due to their root systems being already established in the soil. Usually answers contained a reference to the light penetrating following tree felling so that seeds could germinate and the idea that succession to a climax community would occur, and little else of any relevance. Hardly any candidates considered that being exposed to light might possibly be advantageous for photosynthesis in the seedling trees. Better candidates realised that more seeds could be transported into the cleared area from the adjacent forest, and that animals might return because new habitats and food would be available for them as the new trees began to grow. 15. Once again, the nitrogen cycle proved a weak link in the understanding of a large number of candidates. (a) More candidates were able to identify process P as denitrification than were able to identify process Q as nitrogen fixation. Nitrification was a common wrong answer, as was ammonification, since this refers to the conversion of nitrogen in organic compounds to ammonia. Hyde Clarendon Sixth Form College 12
  • 13. (b) Many candidates misread the questions and did not realise that they had to account for the apparent discrepancy between the amount of ammonia converted to nitrates and the amount formed by nitrogen fixation. Good candidates knew that ammonia is also formed by the decomposition of proteins, amino acids, urea and other organic compounds in the detritus that contain nitrogen. (c) Most candidates realised that hydrogen is lost and oxygen is gained in the conversion of ammonia to nitrate. 16. (a) Nearly all candidates knew that habitats would be lost, and a good number also knew that this would reduce the number of species in the area. (b) Better candidates realised that both potassium nitrate and ammonium nitrate would release nitrate ions into the soil immediately, and that the ammonium ions in ammonium nitrate could be nitrified by nitrifying bacteria to provide a secondary release of nitrates. Many candidates, however, just did not apply their knowledge to the problem and merely recited chunks of the nitrogen cycle, with some confusion between nitrifying and nitrogen-fixing bacteria. 17. (a) Most candidates knew the mark-release-recapture technique, and were able to describe the various steps. However, they did not always explain the reasons behind the steps. For example, they did not always explain that the released insects should be left for a suitable period of time to allow them to re-integrate with the rest of the population. (b) (i) Nearly all candidates knew that there would be only one degree of freedom. (ii) Most candidates knew that the 0.05 level of probability is that most commonly used in biological analysis to judge statistical significance. (iii) Responses to this section were generally disappointing. Most candidates were unable to reason that, because the value for χ2 is greater than the critical value, then there is a probability of less than one in one thousand that the results are due to chance. They were uncertain as to whether the difference in values of χ2 implied that the differences in results are due to chance or due to some biological cause. They wrote about rejecting a null hypothesis which had not been stated and also merely that ‘the results are statistically significant’. Candidates should be aware of 2 the logic that, if χ is greater than the critical value, there is only a probability of (usually one in twenty) that the results are due to chance as the basis for rejecting any null hypothesis and accepting the experimental hypothesis. (c) (i) A number of candidates realised that some of the biomass produced in photosynthesis would be respired by the plant, but very few actually explained that biomass is lost in the form of carbon dioxide. Most of those who involved respiration in their answers suggested that energy is lost, which is true, but loss of energy does not account for the difference in biomass between gross primary production and net primary production. (ii) Nearly all knew that a higher net primary production would lead to more dead plants and so more food for the detritivorous insects. Hyde Clarendon Sixth Form College 13
  • 14. (iii) Most candidates realised that decomposers would respire compounds from the dead plant remains, releasing carbon dioxide, which could be taken in by plants to be used in photosynthesis. However, only better candidates knew that the decomposers were saprobionts and supplied details of extra-cellular digestion. 18. Essays remain extremely variable in quality. For some candidates they provided the saving grace and did much to redeem the limited quality of the two structured questions. Others proved themselves unable to recall basic A-level knowledge, and produced superficial and poorly constructed accounts. The biggest single factor in limiting the marks awarded was undoubtedly the ability to base the essay on appropriate, detailed biology. Thus, for example, many of the essays on bacteria ignored the detail of genetic engineering, nutrient cycles and cellulose digestion which form a major part of the A-level specification and, instead, centred their essays around such topics as yoghurt and cheese production. Essays generally met the requirements for breadth although some devoted so much time to scene setting and significance that they left themselves little time to consider more fundamental issues. There is little doubt that plans would have helped here but these were often conspicuous by their absence. Given the pressure of writing under examination conditions, the quality of written communication was usually sound, often better than that displayed by the same candidates in Questions 1 and 2. The use of technical language, however, was less impressive and a lack of understanding of the terms “ion” and “bacteria” provided an obvious handicap. Certain topics lent themselves to excellent accounts of how inorganic ions are used physiologically - nerve impulses, nephron function, absorption in the intestine, root pressure and guard cell activity. These topics enabled candidates to demonstrate what they knew and how well they understood the processes concerned. There were many detailed and accurate accounts concerning the roles of sodium and potassium ions in the transmission of nerve impulses and of calcium ions in synaptic transmission. Less able candidates sometimes confused the roles of the ions or the parts played by diffusion and active transport. The movement of ions in the counter- current mechanism of the loop of Henle also appeared frequently, but here confusion over detail was more apparent. The identity of inorganic ions and their names produced problems for some in describing the role of ions in the synthesis of biologically important molecules. Phosphorus, for example, was commonly identified as the ion important in the synthesis of ATP and phospholipid. This lack of understanding occasionally led to much irrelevance, especially where discussing hydrogen bonding in DNA, or the importance of carbon, oxygen and hydrogen in macromolecules. The role of hydrogen ions gave scope for some sound biological detail in descriptions of the role of reduced coenzymes in photosynthesis and respiration. Few candidates considered the importance of hydrogen ions in changing the pH environment of enzymes, but many discussed the buffering effect of haemoglobin when describing the transport of carbon dioxide as hydrogencarbonate in the blood. Hyde Clarendon Sixth Form College 14
  • 15. 19. Essays remain extremely variable in quality. For some candidates they provided the saving grace and did much to redeem the limited quality of the two structured questions. Others proved themselves unable to recall basic A-level knowledge, and produced superficial and poorly constructed accounts. The biggest single factor in limiting the marks awarded was undoubtedly the ability to base the essay on appropriate, detailed biology. Thus, for example, many of the essays on bacteria ignored the detail of genetic engineering, nutrient cycles and cellulose digestion which form a major part of the A-level specification and, instead, centred their essays around such topics as yoghurt and cheese production. Essays generally met the requirements for breadth although some devoted so much time to scene setting and significance that they left themselves little time to consider more fundamental issues. There is little doubt that plans would have helped here but these were often conspicuous by their absence. Given the pressure of writing under examination conditions, the quality of written communication was usually sound, often better than that displayed by the same candidates in Questions 1 and 2. The use of technical language, however, was less impressive and a lack of understanding of the terms “ion” and “bacteria” provided an obvious handicap. Although this option was frequently chosen by less able candidates, overall it was impressive to note that many identified the key areas of nutrient cycling, mutualistic relationships and the uses of bacteria in enzyme production and genetic engineering, and wrote about these topics with conviction. Better candidates demonstrated a good understanding of the role of bacteria in the nitrogen cycle although the origins of ammonia were often omitted from discussion. A number of frequent misconceptions studded the work of less able candidates; denitrifying bacteria were considered to play a key role in “restarting” the cycle, nitrifying bacteria commonly converted nitrates to ammonium compounds and nitrogen fixation involved the conversion of “nitrogen in lightning” to nitrates. There were many excellent accounts of the role of bacteria in ruminant digestion. The best of these not only considered cellulose hydrolysis but also discussed the production of fatty acids, and the conversion of ammonia to urea and its subsequent secretion in saliva. More limited candidates confined their answers largely to the bacterial digestion of cellulose. These accounts were characterised by superficiality rather than by error. Genetic engineering provided a third line of approach and, again, there were some very good accounts where candidates showed a secure understanding of basic principles. The less convincing accounts showed confusion over detail. Not infrequently, the role of restriction enzymes was held to be to remove sections of DNA from plasmids, and many considered the role of genetically engineered bacteria to be in producing many copies of the gene coding for insulin. In addition, some of the terminology was heavily reliant on the use of molecular “glue” and “scissors” than on the relevant enzymes. Some good accounts were subsequently spoilt by the introduction of poorly remembered examples from the food industry: yoghurt, cheese and, not infrequently, yeast in the production of alcohol. Hyde Clarendon Sixth Form College 15
  • 16. 20. (a) Too many candidates saw two empty boxes in the flowchart in (i) and either wrote the names of both substances in the boxes or the number of carbon atoms in each substance. This clearly is the result of not reading the question carefully. Those who did answer the question set, usually scored both marks. In part (ii) good candidates realised that all ATP is produced in mitochondria, except that produced in glycolysis. They therefore arrived at the correct answer of 36 ATP by deducting 2 from the net total yield of 38 ATP per molecule of glucose, or by deducting 4 from the total production of 40 ATP. Others did arrive at the correct answer by working out where each molecule of ATP was produced, but many attempting this method did so in a disorganised way and so made errors in calculation. In (iii) most candidates knew that, in the absence of oxygen, some of the reactions of respiration could not take place, but many were unable to describe the extent of anaerobic respiration. Well prepared candidates were able to state clearly that only glycolysis would take place and, therefore, the ATP production of the Krebs cycle and electron transport chain would be lost. They also often (b) Despite being given specific information in part (i) concerning the features of the heterocysts (thick walls and the absence of chlorophyll), and the requirements of nitrogen fixation (anaerobic conditions) candidates too often invented other features and reasons other than maintaining anaerobic conditions for those features. Disappointingly few candidates confined themselves to answers based on excluding oxygen and not producing oxygen, which would inhibit the process of nitrogen fixation. There were some excellent answers to part (ii) from candidates who appreciated that nitrogen-containing compounds in the rice plants would be the starting point for the reactions of the nitrogen cycle, and duly described the roles of decomposition and nitrification accurately and logically. Some realised that the decomposers would produce carbon dioxide as a result of their respiration and that this could be used in photosynthesis by the leaves of the rice plants. However, too many just assumed that the ammonia produced by the heterocysts would be released into the soil, apparently unused by the fern and, in their answers, took this as the starting point for the nitrogen cycle. This clearly shows less appreciation of the situation as described. 21. (a) In (i), almost everybody sketched the pyramid correctly. Some did not draw the pyramid in the conventional way; in effect they drew it upside down, but still showed the correct relationships between the trophic levels. A few candidates failed to the gain the mark because they did not label their diagram. Likewise, in (ii), nearly everyone knew that energy is lost between trophic levels and could suggest at least one way in which it is lost. (b) In this question, it was a relatively common failing for candidates to be unable to write about the relevant part of the nitrogen cycle without also trying to include other aspects. In (i), some candidates failed to distinguish between the roles of decomposers and nitrifying bacteria in their answers, phrasing their responses along the lines of “the decomposers and nitrifying bacteria convert the organic substances into ammonia and then to nitrates”. It was also disappointing to read the number of answers that included references to lightning and the Haber process. However, candidates who understood the nitrogen cycle well usually had little problem with this question. There was a general understanding in (ii) that nitrogen-fixing bacteria convert nitrogen gas into a form that is more readily available; however, there was also a widespread misconception that they convert the gas directly into nitrate ions. (c) In (i), a disappointing number of candidates did not read the question carefully and described changes in the populations of both types of bacteria and both types of Hyde Clarendon Sixth Form College 16
  • 17. protoctistans, usually without really explaining the reasons for any of the changes. Good candidates recognised the predator-prey relationship between the dispersed bacteria and the free-swimming protoctistans in the way the numbers increased and then declined slightly out of phase with each other. In (ii), candidates who understood the process of succession were generally able to recognise the changes in the environment in the treatment tank that resulted in changes in the community inhabiting that environment. A common failing was not to make clear that it is the activities of the organisms that inhabit an area that change the environment and so make it suitable for colonisation by other species. 22. Unit 8 It is difficult to comment meaningfully on the standard of the essays this year compared to that in previous years. Essays ranged from the outstanding to the extremely weak and their quality appeared at times to be independent of the quality of the responses to the other questions in the paper. Thus, there were some excellent answers to questions 1 and 2 which were followed by extremely limited essays and, at the other extreme, papers whose sole redeeming feature was a competent essay. Particularly apparent this year were the many essays which showed no evidence whatsoever of a plan. They were often poorly organised and lacked coherence, with timing often presenting an additional problem. Many candidates still see length in itself as a virtue. Such candidates often produced scripts with two or three extra sheets attached. They often started with some promise but frequently incorporated much that at best could only be regarded as being of marginal relevance. There were some outstanding essays on this topic where the detail throughout was fully in keeping with what might be expected of an A-level candidate, and which did not stray from the theme of the relationship between structure and function. Such essays were a pleasure to read and mark. Others, although sound and attracting considerable credit, tended to belabour particular aspects unnecessarily. A frequent example of this was in protein structure where the concept of shape and fit was illustrated at considerable length with enzymes, hormones, carriers, and antibodies. All were correct and each was relevant but, taken together, they contributed little more than could be provided by considering one example. This approach not infrequently led to candidates discussing one particular polymer at great length at the expense of others, thus affecting the overall balance of the essay. At the other end of the spectrum, it was apparent that some candidates were uncertain as to the meaning of the term ‘polymer’. It was not unusual to see substantial paragraphs written about lipids, and there were also frequent references to “small polymers such as glucose and maltose”. Apart from this, errors often arose from a poor understanding of technical terminology. Thus a- and P-glucose were regularly confused; cellulose molecules were described as consisting of p-pleated sheets, and there was considerable uncertainty over the relationship between amino acids and DNA, the latter all too frequently being described as a polymer of the former. Hyde Clarendon Sixth Form College 17
  • 18. Unit 9 The essays varied greatly in length and biological content, but less so in breadth and relevance. Many essays showed good evidence of planning in a way which would clearly be useful to the author, and which kept their work relevant. Some candidates failed to plan at all. The competence with which the scientific terminology was used was pleasing, with many technical terms being used in the majority of essays. Weaker candidates tended to relate their work in everyday language, dwelling on the distribution of large surface areas rather than on the link with transfer processes. Many of the candidates who chose to write this essay showed themselves to be competent biochemists. They understood the nature of a polymer, and frequently opened their essay with a definition. Some of the best candidates were at pains to point out that lipids were not polymers, and so would not be discussing them. Sadly, some thought they were, and included as much on this group of substances as they did on the relevant ones. The most frequently discussed polymers were proteins and carbohydrates. Extensive and detailed knowledge of the structure of proteins was included, with descriptions of peptide bonds, primary to quaternary structures, and the bonds which hold them together. Weaker candidates were somewhat haphazard in their descriptions, but the better ones were not only capable of demonstrating a lot of detail but their approach was highly logical. The links with function were less competent. Some simply offered a selection of proteins, while the best essays discussed the differences, for example, between those with a simple secondary structure compared with the globular examples. Frequent choices were enzymes and haemoglobin. Other interesting additions included keratin, antibodies and hormones; although most felt there was no need to comment on the fact that not all hormones are proteins. As a consequence a few lipids were mentioned. The work on the structure was in far more detail than the function in many cases, leaving candidates missing the opportunity to demonstrate detailed knowledge of the control of blood glucose, the principles of immunology, and the transport of blood gases. This was a route which could have been exploited by those capable biologists whose biochemistry is perhaps a little sketchy. The work on the structure of carbohydrates was equally detailed by a lot of candidates who chose this essay. However, it was much more often that the function of the molecule was linked very closely to its structure. Starch and glycogen were described as large, compact molecules while the work on cellulose reflected its role in the structure of a plant. There was some confusion over the solubility of some of these molecules. Candidates who moved on to discuss the nucleic acids were less frequently seen. Those that did gave good descriptions of their structure, but a number went on to give huge amounts of detail about the processes in which they play a part, rather than making the more careful links to their function. Full details of protein synthesis, DNA replication, and genetic engineering, were inappropriate. Weaker candidates confused nucleic acids with proteins. Hyde Clarendon Sixth Form College 18
  • 19. It was easy for candidates to use this essay to show their A-level knowledge. Some candidates tried to use the outline of an essay they may have written earlier on a single polymer. There was a wide range of marks, with a good number of the better candidates gaining full or close to full marks. 23. Part (a)(i) appeared to indicate a problem over the term ‘organic’, as the most common incorrect answers were ammonia and nitrate. In part (ii) the process of death or decay was often given rather than a source of the material. Nitrifying, nitrogen fixing and denitrifying bacteria caused confusion in answers to part (b); a substantial number of candidates did not know which type of bacteria did what. Although this flow diagram was not the usual form of the nitrogen cycle, enough clues were given to allow candidates to relate it to their own knowledge, and many were able to do so. 183 was a common incorrect answer to part (c), obtained by deducting the value for ‘cattle’ from that for inorganic nitrogen’. Many gave 339 which suggested that they had the principle correct, but missed out the effect of fixation in providing some of the ‘nitrogen’ requirements of clover. While a lot of the candidates knew that clover had nitrogen fixing bacteria in root nodules, a significant number thought that the clover did its own nitrogen, fixing in part (d). A common circular argument was that cattle eat the clover, add the nitrogen from the clover to the soil via dung and urine to benefit the grass which is an advantage to the cattle. Only a few suggested that growing clover might save the farmer having to buy and apply fertiliser. 24. ‘Ammonia’ rather than ammonium was a common response in part (b). Although many candidates made correct references to nitrifying bacteria in part (b), few were unable to give even a GCSE level response to the processes involved in the production of ammonium ions. 25. Three times as many candidates attempted the essay on cycles in biology as on the structure and function of carbohydrates. As expected, the carbohydrates essay tended to be shorter but there was no evidence to suggest that this title attracted less able candidates. Most candidates provided some form of plan, although these varied greatly in length and detail. Fortunately, compared to last year, fewer candidates spent a disproportionate amount of their time on a plan to the detriment of the essay itself. There was some evidence to support the view that candidates were better prepared for the essays, particularly in terms of providing relevant information and breadth of knowledge. Most examiners commented on a general improvement from last year although quality of written communication and poor handwriting remains a problem for a significant number of candidates. This essay title enabled candidates to include a wide range of topics from several modules. As with essay (a), content beyond the specification, and to a greater extent from the option modules, was also credited. Almost invariably candidates included the carbon and nitrogen cycles in their answers. The weakest candidates limited their discussions to superficial accounts of these nutrient cycles and often the water cycle, with few if any factual details of A level standard. However, most candidates were able to provide a greater breadth of knowledge and included metabolic, physiological and in some cases life cycles in their essays. Hyde Clarendon Sixth Form College 19
  • 20. The quality of written communication in relation to nutrient cycles was particularly disappointing. Many candidates referred to animals obtaining ‘nitrogen’ from plants, and decomposers releasing ‘carbon’ and ‘nitrogen’. Plants ‘breathing in gases’ and energy being ‘produced’ or ‘recycled’ were not uncommon phrases. Many candidates suggested that nitrifying bacteria are involved in nitrogen fixation. There was also some confusion over the role of decomposers with few candidates specifying the nitrogen compounds involved. Many candidates did not display an understanding of nutrient cycles, with some suggesting that the importance of the nitrogen cycle is to maintain the nitrogen content in the air. Most candidates mentioned the Krebs cycle, with many candidates also referring to the light- independent reaction. Descriptions of the Krebs cycle often failed to complete the recycling of the four-carbon compound and tended to concentrate on ATP production. Unfortunately, very few candidates described the cyclical features of the electron transport chain. Similarly, the cyclic nature of ATP formation and breakdown was rarely considered. Nevertheless, there were some excellent accounts of the Krebs cycle which outlined the importance of regenerating a four-carbon compound to react with acetyl coenzyme A. Generally candidates displayed a clearer understanding of the regeneration of RuBP in the light-independent reaction. However, there was still the tendency for candidates to provide incomplete cycles. Discussion of metabolic cycles often led to irrelevancies, particularly glycolysis and non-cyclic features of the light-dependent reaction. Where attempted, candidates provided some worthwhile attempts to convey the cyclical nature of negative feedback, usually in relation to the control of blood glucose. However, a significant number of candidates referred to insulin and glucagon as enzymes and there was some confusion between glycogen and glucagon. Many candidates provided details of the ornithine cycle even though this is not required in this specification. A significant number of candidates, presumably those taking option 8, included the menstrual cycle in their essays. However, many of these candidates included irrelevant information such as details of the process of fertilization. References to synaptic transmission and actomyosin bridge formation as cyclical processes were infrequent. However, when attempted, these descriptions were detailed and comprehensive. This was certainly not the case for descriptions of the cardiac cycle. These accounts were often confused and failed to relate pressure and volume changes in the heart and aorta to events in the cardiac cycle. A significant number of candidates included descriptions of mitosis and meiosis, often towards the end of the essay. These descriptions were often superficial or contained numerous inaccuracies. Candidates were often confused between chromatids and chromosomes and many had difficulty explaining the significance of meiosis in a life cycle. A few candidates provided detailed descriptions of predator / prey cycles or viral replication. 26. (a) In general, this question was not well answered in that too many failed to realise that it was a three-mark question and just gave one idea, rephrased or extended. Surprisingly few candidates recognised that monoculture provides a huge food supply and hence promotes rapid population increase within a pest population. Most recognised that the pest could have an increased effect on the crop in a monoculture. Hyde Clarendon Sixth Form College 20
  • 21. (b) (i) The majority of candidates were able to identify that the ‘resistance’ allele is recessive, that the parents would therefore be heterozygous, and that the resistant offspring would be homozygous recessive. Too many incorrectly referred to mutations resulting in resistance. (ii) Most candidates scored well with bioaccumulation and killing non-target organisms being the most frequently seen correct responses. It was surprising to see a significant number of candidates referring to the pesticide leaching into the water and causing eutrophication. 27. (i) Most candidates gained credit for the fact that the fertiliser had leached into the river, but only the more able explained that it is excess fertiliser that leaches in this way. (ii) The vast majority of candidates scored highly by correctly recounting the standard sequence of events in eutrophication. 28. No Report available for this question. Hyde Clarendon Sixth Form College 21