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CANADIAN RESEARCH FOCUS Interview with Dr. Mehrdad Rafat “ PEG-PLA microparticles for encapsulation and delivery of Tat-EGFP to retinal cells”,  Biomaterials (2010).  31: 3414-3421.  doi:10.1016/j.biomaterials.2010.01.031 May 7 th , 2010 conducted by Patricia Comeau
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Figure 1: Tat-EGFP encapsulated nano/microparticles subretinally injected into the outer nuclear layer of the retina Image courtesy of Rafat, M., University of Ottawa; and Kolb, E., University of Utah, 2010
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Interview with Dr. Rafat  Vision Program, Ottawa Hospital Research Institute and Department of Cellular and Molecular Medicine, University of Ottawa
What is the need for controlled release technologies in the eye?  ,[object Object],…continued on next slide  ->
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What are the key target diseases? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
How and where are the microparticles injected into the retina? ,[object Object],…continued on next slide  ->
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What other characteristics of the microparticles, aside from size and morphology, influence subretinal delivery? ,[object Object]
Clinically, what would you replace Tat-EGFP with to treat a retinal disease? ,[object Object],…continued on next slide  ->
[object Object]
Why is there a difference between the cellular uptake at 48 and 96hours? ,[object Object]
How do electroretinograms determine the biocompatibility of micro-particles? ,[object Object],…continued on next slide  ->
[object Object]
Does the presence of the microparticles in the retina pose any potential risks?  ,[object Object]
When do you plan for the particles to release the drug? ,[object Object]
How are the polymer degradation products removed from the eye? ,[object Object]
What major hurdles remain to be overcome before patients can benefit from its application? ,[object Object],…continued on next slide  ->
[object Object],[object Object]
CC-CRS Question #8 Thank you for the interview!
Dr. Mehrdad Rafat Biography of
[object Object],…continued on next slide  ->
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[object Object]

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Non-viral ocular delivery

  • 1. CANADIAN RESEARCH FOCUS Interview with Dr. Mehrdad Rafat “ PEG-PLA microparticles for encapsulation and delivery of Tat-EGFP to retinal cells”, Biomaterials (2010). 31: 3414-3421. doi:10.1016/j.biomaterials.2010.01.031 May 7 th , 2010 conducted by Patricia Comeau
  • 2.
  • 3.
  • 4. Figure 1: Tat-EGFP encapsulated nano/microparticles subretinally injected into the outer nuclear layer of the retina Image courtesy of Rafat, M., University of Ottawa; and Kolb, E., University of Utah, 2010
  • 5.
  • 6. Interview with Dr. Rafat Vision Program, Ottawa Hospital Research Institute and Department of Cellular and Molecular Medicine, University of Ottawa
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23. CC-CRS Question #8 Thank you for the interview!
  • 24. Dr. Mehrdad Rafat Biography of
  • 25.
  • 26.
  • 27.