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Nucleus: structure and function

Submitted to: Dr. Mohammad Saeed
Submitted by: Sami ur rehman
Submitted date:11/11/2013
• Nucleus
• Nuclear Envelope
• Nuclear Components
 Nucleoplasm
 RNA and RNA protéine complexes

• Nucleolus
• ORGANISATION OF DNA IN CHROMOSOMES
 Nucléosomes and Chromatin'Structure

• REPLICATION OF DNA
 Semi-conservative Replication
 Replication Fork

2
• Enzymes Involved in DNA Replication
• Synthesis of Okazaki Fragments
• TRANSCRIPTION
 RNA Synthesis in Procaryotes
 RNA Synthesis in Eucaryotes

• Different Types of RNA
• RNA PROCESSING IN EUCARYOTES
 Modification of mRNA
 Removal of Introns
3
Lysosomes: fusion and function
•
•
•
•
•
•

Lysosomes
The discovery of lysosomes
Membrane traffic routes to lysosomes
Endosome fusion with lysosomes
Delivery to lysosomes and lysosomal fusion.
Electron microscopy of endosome–lysosome
fusion.
4
• Tethering

• Trans-SNARE complex formation.
• Hybrid organelles.
• Reformation of lysosomes from hybrids.
• Membrane fusion.
5

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Nucleus

  • 1. Nucleus: structure and function Submitted to: Dr. Mohammad Saeed Submitted by: Sami ur rehman Submitted date:11/11/2013
  • 2. • Nucleus • Nuclear Envelope • Nuclear Components  Nucleoplasm  RNA and RNA protéine complexes • Nucleolus • ORGANISATION OF DNA IN CHROMOSOMES  Nucléosomes and Chromatin'Structure • REPLICATION OF DNA  Semi-conservative Replication  Replication Fork 2
  • 3. • Enzymes Involved in DNA Replication • Synthesis of Okazaki Fragments • TRANSCRIPTION  RNA Synthesis in Procaryotes  RNA Synthesis in Eucaryotes • Different Types of RNA • RNA PROCESSING IN EUCARYOTES  Modification of mRNA  Removal of Introns 3
  • 4. Lysosomes: fusion and function • • • • • • Lysosomes The discovery of lysosomes Membrane traffic routes to lysosomes Endosome fusion with lysosomes Delivery to lysosomes and lysosomal fusion. Electron microscopy of endosome–lysosome fusion. 4
  • 5. • Tethering • Trans-SNARE complex formation. • Hybrid organelles. • Reformation of lysosomes from hybrids. • Membrane fusion. 5