2. Overview
• Nitrogen is critical.
– In everything basically
• Luckily very abundant.
• Unluckily it’s hard to get at.
– Most is N≡N in air.
• Methods of Acquisition
1. Nitrate based Fertilizer.
2. Retrieval from
decomposition.
3. Natural atmospheric
conversation.
4. Nitrogen Fixation.
3. Nitrogen Fixation, and our way around it
• Beijerinck 1901.
– Nitrogenase to
the rescue!
– Prokaryotes, th
e unsung
champions of
life.
• Agricultures way
around it.
– Use a crap ton
of fertilizer.