1. The document discusses QuEChERS sample preparation and cleanup methods using different sorbents to remove interferents like fatty acids, pigments, and sterols from pesticide extracts.
2. It evaluates the effectiveness of a new engineered carbon material called CarbonX compared to traditional glass-backed carbon sorbent for removing contaminants while maintaining high pesticide recoveries and minimizing column and liner fouling during GC/MS analysis.
3. The CarbonX material showed superior recoveries of planar pesticides above 75% and was able to remove extreme fatty acid contaminants from botanical extracts, demonstrating its potential to handle difficult sample matrices compared to other cleanup methods.
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Quechers cleanup presentation_itsp
1. QuEChERS Sample Prep and its
Cleanup Effectiveness and
Efficiency – A Look at Sorbents
2. QuEChERS
1. Extraction of Pesticides With Acetonitrile, MgSO4 and Salt
Cleanup of Fatty Acids with
Primary Secondary Amine
Cleanup of
Pigments with
GCB
Cleanup of Sterols and
Non-polar interferents
with C18 Silica
2. Dispersive Solid Phase Extraction (dSPE) Cleanup of Interferents
O
OH
O
OHH3C
H3C
Palmitic Acid
Stearic Acid
Chlorophyll a
Campesterol
3. Pigmented Sample Issues
• Liner Fouling
• Column Retention Time
Shortens
• Peak Tailing
Carrot Pea Pineapple Raspberry
Liner with 50
Injections of
Spinach Extract
after dSPE
cleanup with
GCB
5. 0.0 20.0 40.0 60.0 80.0 100.0 120.0
Chlorothalonil
Chlorpyrifos-methyl
DCBP
Chlorpyrifos
Folpet
Coumaphos
Recovery (%)
N
N
ClCl
Cl
Cl
N
O
O
S
C
Cl
Cl Cl
Current Solution – GCB
6. A New Engineered Carbon Material
CarbonX GCB
Before AfterAfter
Effect of Glass Microscope Slide
Weight on Particle Diameter
Intact particles
after grinding
Crushed Particles
Pore Diameter (A)
PoreVol(cm3/g)
Repeatable and Reproducible Pore Structure
80 A avg pore
0
0.2
0.4
0.6
0.8
1
1.2
0 100 200 300 400
CarbonX
GCBSPE1
Time(min)
Time to elute 4 mL of
CH2Cl2 from
3 mL SPE Cartridge
under gravity flow.
N= 10. GCB cartridge
is commercially
packed & sourced.
More Reproducible Flow Characteristics
100 um
7. Color results from 3 cleanup styles
GCBGCB w/ Tol
CarbonX
Plus
Spinach
Extract
15. Conclusions
• Superior QuEChERS Recoveries with
Engineered Carbon for GC/MS
• Similar Color Removal
• Greater than 75% recovery for planar pesiticides
• Toluene Unnecessary
• Decreased Liner and Column Fouling
• Sometimes Extreme Issues Can Necessitate
Extreme Cleanup Solutions
• Miniaturization Can Assist when Sample Volume
is an Issue or Automation is Desired
Confidential
16. Dr. Jon Thompson
United Science
Dr. Chunze Lai
R&D
Dr. Li Chen
R&D
Dr. Conor Smith
R&D
Prof. Dwight Stoll
Gustavus
Doug Fryer
Product Manager