2. Why is cleaning
important?
• House hold surfaces are very good
breeding grounds for bacteria especially
the kitchen.
• A single cell of bacteria can multiply into
8 million cells within 24 hours.
• Common illnesses such as the flu and
cold are preventable by cleaning.
3. Lysol
• Lysol claims to be effective against Avian
Influenza, Herpes, E. Coli, Staphlococcus
Aureus, and Pseudomonas Aeruginosa.
4. Pine-Sol
• Claims to be effective against
Staphlococcus Aureus, Salmonella
Choleraesuis, and athletes foot fungus.
5. Hypothesis
• After reading each product description
Lysol claims to kill a larger variety of
bacteria.
• Therefore we will hypothesize that Lysol
will be a more effective anti bacterial.
6. Experimental Design
• Using two different methods: standard
Kirby Bauer and a modified version Use
Dilution Assay.
• Using three different types of bacteria to
represent a large population of bacteria.
Gram Negative (E. Coli), Gram Positive (S.
Aureus), P. Aeruginosa (Resistant).
7. Use Dilution Assay
• Using 3 TSA Broths a bacteria culture was
grown.
• The culture was poured into a beaker with
more TSA broth.
• 10mL of each disinfectant was poured into
the culture.
• Samples were taken at 0,1, 2, 3, 4, 5, 8, 10
• Samples were streaked on a TSA plate and
observed.
8. Kirby Bauer
• Using 3 TSA plates, a lawn of each
respective bacteria was spread.
• 2 Sterile disc were soaked in a disinfectant.
• The disc were then dropped onto the TSA
plate and observed.
9. Predicted Outcomes:
Kirby Bauer
• Lysol will create a larger zone of inhibition
for E. Coli, S. Aureus, and P. Aeruginosa.
10. Predicted Outcomes:
Use Dilution Assay
• Lysol will kill E. Coli, S. Aureus, and P.
Aeruginosa faster than Pinesol.
• Pinesol will only kill S. Aureus.
11. Results: Use Dilution
Assay (Lysol)
• Growth in P. Aeruginosa and Ecoli through
10 minutes.
• Growth halts after 2 minutes for S. Aureus
12. • Growth in P. Aeruginosa and S. Aureus
through 10 minutes.
• Growth halts after 10 minutes for E. Coli
13. • E. Coli - Lysol showed a 6mm ZOI while
Pinesol showed a 8mm ZOI
• P. Aeruginosa - Both showed a 6mm ZOI.
• S. Aureus - Lysol showed a 6mm ZOI and
Pinesol showed a 10mm ZOI.
14. Conclusions
• In the Use Dilution Assay Lysol we can
extrapolate that Gram Positive bacteria
while Pinesol only kill Gram Negative
bacteria.
• In the Kirby Bauer test Lysol showed a
larger ZOI in both Gram Positive and
Negative bacteria.
• It’s a DRAW!
