Présentation conjointe CRNS/Biozone Europe au congrès européen de virologie en 2008.

1. Cold oxygen plasma technology efficiency against airborne
respiratory viruses
Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1
VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de
1
Médecine Laënnec, 69372 Lyon France
2
Biozone Europe, France

2. What is plasma?
Fourth state of matter
Plasma = ionized gas
Man-made plasma: many ways to make
plasma
⇒“Cold” plasma
Energy by radio-frequency, microwave
or UV-light…
Plasma as a sterilizing agent
(Menashi, 1968)
Oxygen plasma efficiency on B. subtilis and Clostridium sporogenes (Nelson
and Berger, 1989)

3. BiozoneTM technology
Cutting edge air & surface purification technology
Combination of : - Deep ultraviolet light,
- Photo-catalytic oxidation
- Cold oxygen plasma (COP)
Photo-Catalytic oxidation process destroys air pollutants on surfaces
COP eliminates reproductive capability of micro-organisms
Deep UV light sterilizes micro-organisms that pass through the machine
Negative ions decrease total concentration of harmful chemicals in the air
Ozone produced, obliterates bacteria and fungi and remove odour from the air

4. Airborne respiratory viruses
RSV and hPIV-3
Most important pathogens encountered in low respiratory tract
infections in infants and small children (bronchiolitis and pneumonia)
Common reinfections among children and adults (Upper respiratory
tract infection).
Infection transmitted by contact with infectious material and by aerosol
(droplets)
Influenza
Responsible of recurrent annual epidemics causing acute febrile
respiratory illness
Emerging of avian Influenza H5N1: potential threat for a pandemic
Mild to severe illness, all age group
Infection by aerosol (droplets)

5. Objective
Quantify the impact of deep ultraviolet light and cold oxygen plasma
(COP) technologies on viral decontamination of several respiratory
viruses
Human respiratory syncytial virus (RSV)
Human parainfluenza type 3 virus (hPIV-3)
Avian Influenza A/H5N2
Classic germicide UV-light lamp
Device BioZoneTM UV-light Lamp (Cold 02 plasma + germicide UV)
External BioZoneTM UV-light Lamp (Cold 02 plasma only)

6. Study Design
BSL3 Lab
RSV-A Long strain (ATCC VR-26)
hPIV-3 C-243 strain (ATCC VR-93)
H5N2 A/Finch/England/2051/91 strain
Viral production
Purification/concentration
UV/COP
Device
Nebullization
Air flow
Droplets
Upstream Downstream
outlet outlet
Viral titration
(TCID50/mL)
Flow velocity 0,9m/s
Reaction Time 0,44s

7. Results/1
Impact of UV and cold oxygen plasma on viral decontamination of hPIV-3
Downstream
TICD50/mL 1,00E+10 outlet
Initial
Upstream
1,00E+09
outlet No
1,00E+08 treatment
1,00E+07
1,00E+06
BioZoneTM
1,00E+05
Classic COP only
UV 7 log
1,00E+04
1,00E+03
1,00E+02 BioZoneTM
COP and UV
1,00E+01
1,00E+00
Comparable effects of UV and COP (4 and 3,6 log destruction rate)
Combination of UV and COP (BioZoneTM Lamp) allow high destruction
of hPIV-3: 7 log destruction rate, >99,99% in 0,44s

8. Results/2
Impact of UV and cold oxygen plasma on viral decontamination of RSV
Downstream
1,00E+09
outlet
TICD50/mL Initial
1,00E+08
Upstream No
outlet treatment
1,00E+07
1,00E+06 Classic BioZoneTM
UV COP only 3,8 log
1,00E+05
BioZoneTM
1,00E+04
COP and UV
1,00E+03
1,00E+02
1,00E+01
1,00E+00
Comparable effects of UV and COP (2,1 and 2 log destruction rate)
Combination of UV and COP (BioZoneTM lamp) allow high destruction
of RSV: 3,8 log destruction rate, >99,99% in 0,44s

9. Results/3
Impact of UV and cold oxygen plasma on viral decontamination of H5N2
TICD50/mL 1,00E+09
Initial Downstream
1,00E+08
outlet
1,00E+07
Upstream
1,00E+06 outlet
1,00E+05 No
treatment
1,00E+04
1,00E+03
Classic BioZone
TM 5,5 log
1,00E+02 COP only
UV
1,00E+01 BioZoneTM
COP and UV
1,00E+00
Comparable effects of UV and COP (3,5 and 2,9 log destruction rate)
Combination of UV and COP (BioZoneTM lamp) allow high destruction of
H5N2: 5,5 log destruction rate, >99,99% in 0,44s

10. Conclusions and Perspectives
Difference of results between viruses could be explain by virus
properties (structure of viral envelope) or viral suspension
preparation.
High efficiency of BiozoneTM lamp in viral decontamination
⇒ Future tests with other viruses (rhinovirus, hepatitis virus…)
⇒ Surface decontamination, Photocatalytic oxidation parameter
Cold oxygen plasma technology could be an innovatory and efficient
way to decontaminate air to protect human populations against
respiratory viruses
=> Decontamination of hospital wards, BSL3 labs…

11. Acknowledgements
We thank all the Staff at Centre de biologie et de Pathologie
Est clinical virology lab (Lyon, France) and all the staff of
VirPath lab (Bruno Lina’s group, Lyon, France)