Cold oxygen plasma technology efficiency against airborne
                       respiratory viruses

    Terrier O.1, Col...
What is plasma?

                                                        Fourth state of matter
                          ...
BiozoneTM technology

 Cutting edge air & surface purification technology
         Combination of : - Deep ultraviolet lig...
Airborne respiratory viruses


          RSV and hPIV-3
          Most important pathogens encountered in low respiratory ...
Objective



    Quantify the impact of deep ultraviolet light and cold oxygen plasma
    (COP) technologies on viral deco...
Study Design
                                                                               BSL3 Lab

                    ...
Results/1
Impact of UV and cold oxygen plasma on viral decontamination of hPIV-3


                                       ...
Results/2
Impact of UV and cold oxygen plasma on viral decontamination of RSV


                                          ...
Results/3
Impact of UV and cold oxygen plasma on viral decontamination of H5N2



    TICD50/mL   1,00E+09
               ...
Conclusions and Perspectives

   Difference of results between viruses could be explain by virus
   properties (structure ...
Acknowledgements




       We thank all the Staff at Centre de biologie et de Pathologie
       Est clinical virology lab...
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Escv 2008 Cnrs Biozone

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Présentation conjointe CRNS/Biozone Europe au congrès européen de virologie en 2008.

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Escv 2008 Cnrs Biozone

  1. 1. Cold oxygen plasma technology efficiency against airborne respiratory viruses Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1 VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de 1 Médecine Laënnec, 69372 Lyon France 2 Biozone Europe, France
  2. 2. What is plasma? Fourth state of matter Plasma = ionized gas Man-made plasma: many ways to make plasma ⇒“Cold” plasma Energy by radio-frequency, microwave or UV-light… Plasma as a sterilizing agent (Menashi, 1968) Oxygen plasma efficiency on B. subtilis and Clostridium sporogenes (Nelson and Berger, 1989)
  3. 3. BiozoneTM technology Cutting edge air & surface purification technology Combination of : - Deep ultraviolet light, - Photo-catalytic oxidation - Cold oxygen plasma (COP) Photo-Catalytic oxidation process destroys air pollutants on surfaces COP eliminates reproductive capability of micro-organisms Deep UV light sterilizes micro-organisms that pass through the machine Negative ions decrease total concentration of harmful chemicals in the air Ozone produced, obliterates bacteria and fungi and remove odour from the air
  4. 4. Airborne respiratory viruses RSV and hPIV-3 Most important pathogens encountered in low respiratory tract infections in infants and small children (bronchiolitis and pneumonia) Common reinfections among children and adults (Upper respiratory tract infection). Infection transmitted by contact with infectious material and by aerosol (droplets) Influenza Responsible of recurrent annual epidemics causing acute febrile respiratory illness Emerging of avian Influenza H5N1: potential threat for a pandemic Mild to severe illness, all age group Infection by aerosol (droplets)
  5. 5. Objective Quantify the impact of deep ultraviolet light and cold oxygen plasma (COP) technologies on viral decontamination of several respiratory viruses Human respiratory syncytial virus (RSV) Human parainfluenza type 3 virus (hPIV-3) Avian Influenza A/H5N2 Classic germicide UV-light lamp Device BioZoneTM UV-light Lamp (Cold 02 plasma + germicide UV) External BioZoneTM UV-light Lamp (Cold 02 plasma only)
  6. 6. Study Design BSL3 Lab RSV-A Long strain (ATCC VR-26) hPIV-3 C-243 strain (ATCC VR-93) H5N2 A/Finch/England/2051/91 strain Viral production Purification/concentration UV/COP Device Nebullization Air flow Droplets Upstream Downstream outlet outlet Viral titration (TCID50/mL) Flow velocity 0,9m/s Reaction Time 0,44s
  7. 7. Results/1 Impact of UV and cold oxygen plasma on viral decontamination of hPIV-3 Downstream TICD50/mL 1,00E+10 outlet Initial Upstream 1,00E+09 outlet No 1,00E+08 treatment 1,00E+07 1,00E+06 BioZoneTM 1,00E+05 Classic COP only UV 7 log 1,00E+04 1,00E+03 1,00E+02 BioZoneTM COP and UV 1,00E+01 1,00E+00 Comparable effects of UV and COP (4 and 3,6 log destruction rate) Combination of UV and COP (BioZoneTM Lamp) allow high destruction of hPIV-3: 7 log destruction rate, >99,99% in 0,44s
  8. 8. Results/2 Impact of UV and cold oxygen plasma on viral decontamination of RSV Downstream 1,00E+09 outlet TICD50/mL Initial 1,00E+08 Upstream No outlet treatment 1,00E+07 1,00E+06 Classic BioZoneTM UV COP only 3,8 log 1,00E+05 BioZoneTM 1,00E+04 COP and UV 1,00E+03 1,00E+02 1,00E+01 1,00E+00 Comparable effects of UV and COP (2,1 and 2 log destruction rate) Combination of UV and COP (BioZoneTM lamp) allow high destruction of RSV: 3,8 log destruction rate, >99,99% in 0,44s
  9. 9. Results/3 Impact of UV and cold oxygen plasma on viral decontamination of H5N2 TICD50/mL 1,00E+09 Initial Downstream 1,00E+08 outlet 1,00E+07 Upstream 1,00E+06 outlet 1,00E+05 No treatment 1,00E+04 1,00E+03 Classic BioZone TM 5,5 log 1,00E+02 COP only UV 1,00E+01 BioZoneTM COP and UV 1,00E+00 Comparable effects of UV and COP (3,5 and 2,9 log destruction rate) Combination of UV and COP (BioZoneTM lamp) allow high destruction of H5N2: 5,5 log destruction rate, >99,99% in 0,44s
  10. 10. Conclusions and Perspectives Difference of results between viruses could be explain by virus properties (structure of viral envelope) or viral suspension preparation. High efficiency of BiozoneTM lamp in viral decontamination ⇒ Future tests with other viruses (rhinovirus, hepatitis virus…) ⇒ Surface decontamination, Photocatalytic oxidation parameter Cold oxygen plasma technology could be an innovatory and efficient way to decontaminate air to protect human populations against respiratory viruses => Decontamination of hospital wards, BSL3 labs…
  11. 11. Acknowledgements We thank all the Staff at Centre de biologie et de Pathologie Est clinical virology lab (Lyon, France) and all the staff of VirPath lab (Bruno Lina’s group, Lyon, France)

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