The document discusses the generation and husbandry of transgenic mice. It describes how transgenic mice are produced by injecting DNA into fertilized mouse eggs, which can integrate randomly and potentially disrupt endogenous genes. It also describes targeted "knockout" techniques using embryonic stem cells. The document provides details on planning and maintaining transgenic mouse colonies, including breeding strategies, housing, health monitoring and common pathogens.

1. Transgenic mice: generation and husbandry

7. Brinster's growth hormone mouse

8. Trangenic mouse embryo in which the promoter for a gene expressed in neuronal progenitors (neurogenin 1) drives expression of a beta-galactosidase reporter gene. Neural structures expressing the reporter transgene are dark blue-green. (Dr. Anne Calof)

9. GFP transgenic mouse (Nagy) Tail tip 9.5 day embryos - GFP and wt

10. GFP transgenic mouse (Nagy)

15. Pronuclear injection

17. Implanting 1(or 2) cell embryos 1 2

18. Implanting 1(or 2) cell embryos (cont.) 3

20. Vasectomizing 1 2

27. Typical KO vector *tk:thymidine kinase

29. ES cells growing in culture

32. Successfully transformed ES cells are injected into blastocysts

34. Implanting blastocysts 1 2

35. Implanting blastocysts (cont.) 3 4

36. Littermates Black mouse - no apparent ES cell contribution Chimeric founder - strong ES cell contribution Chimeric founder - weaker ES cell contribution

37. Chimeric mouse

60. The End and Good bye!