This document discusses accelerating rapid diagnostics using new detection methods. Traditional diagnostic workflows can take 2+ days but detecting pathogens faster could improve patient outcomes and reduce costs. LumiByte is developing new methods like MuScan for single-cell detection and Colony Tracker for monitoring microbial growth that can provide results in hours rather than days. Faster diagnostics are needed to combat antimicrobial resistance by improving treatment and reducing inappropriate antibiotic use.

1. Accelerating Rapid Diagnostics
Vivian Monteban, September 2013

2. LumiByte
We catch microbes
accelerating rapid diagnostics
ultra fast
automated
optical
bacterial detection
Saving time = Saving lives
2sep-13

3. Clinical microbiology
• Shorten time till definitive treatment will:
– Decreased morbidity & mortality
– Decreased costs –
• decreased use of drugs,
• the need for isolation, and
• the use of additional laboratory and other
diagnostic studies.
– Shorter duration of stay in hospital
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4. Traditional workflow
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Sample collected
Microscopy
(often uninformative for
clinical purposes)
Pathogen(s) cultures
Antibiotic sensitivity
determined
(complex resistances may need
further confirmation)
Empirical
therapy
Definitive
therapy
Minutes to hours
24-48 hours*
24-48 hours*
*longer for slow growers e.g.
Mycobacterium spp.
•Total time: more then 2 days!
•Amplification increases error
•Can be difficult to take samples
100% sterile
•Speed up individual stages
•Combine
•Improve Emperical therapy
•Shorten time till definitive
treatment

5. sep-13 5
Sample collected
Sample enrichment/
Culturing
Antibiotic suseptibility
determined
(complex resistances may need
even further confirmation)
Day(s) to weeks*
Day(s) to weeks*
Report for QC
Therapy

6. sep-13 6
Day(s) to weeks*
Day(s) to weeks*
Report for QC
Antibiotic suseptibility
determined
(complex resistances may need
even further confirmation)
Sample enrichment/
Culturing
Sample collected Minutes to hours Report for QCTherapy 2-3 timepoints; 1 day
Therapy

7. Portfolio
• MuScan – single cell detection
• Colony Tracker– growth detection (R&D)
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8. Rapid Detection method
Filtrate Scanning Analyse
10 -60 minutes
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9. MuScan – single cell detection
Applications
• Process monitoring
• Root cause analysis
• Hygiene screening and absence
testing (e.g. endoscopes)
Sample size
• Pure water: >1L
• Blood : currently 1ml
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White light Blue light Amber light Violet light

10. Key facts (WHO)
• Infections caused by resistant microorganisms often fail to respond to
conventional treatment, resulting in prolonged illness, greater risk of
death and higher costs.
• A high percentage of hospital-acquired infections are caused by highly
resistant bacteria such as methicillin-resistant Staphylococcus
aureus (MRSA) or multidrug-resistant Gram-negative bacteria.
• New resistance mechanisms have emerged, making the latest
generation of antibiotics virtually ineffective.
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Antimicrobial resistance
Fact sheet N°194
Updated May 2013

11. Emergence and spread of AMR (WHO)
Important factors that
accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and
monitoring systems;
• inappropriate use of antimicrobial medicines.
• insufficient diagnostic, prevention and therapeutic tools.
• lack of a comprehensive and coordinated response;
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12. Portfolio
• MuScan – single cell detection
• Colony Tracker– growth detection (R&D)
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13. Colony Tracker
The Consept:
• Monitoring individual bacterial micro-colony growth
• Growth based, ultra fast
• Antibiotic susceptibility testing
• Double infections easily recognized
Simplified image analysis
only colonies grow!
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Images provided by:
Alice den Hertog - KIT Biomedical Research

14. KIT - Tuberculosis
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Images provided by:
Alice den Hertog - KIT Biomedical Research

15. KIT - Tuberculosis
• Conditions can be changed after initial
growth detection
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Images provided by:
Alice den Hertog - KIT Biomedical Research

16. Emergence and spread of AMR (WHO)
Important factors that
accelerate the emergence and spread of AMR:
• weak or absent antimicrobial resistance surveillance and monitoring systems;
• inappropriate use of antimicrobial medicines.
• insufficient diagnostic, prevention and therapeutic tools.
• lack of a comprehensive and coordinated response;
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The Colony Tracker provides answers days
before standard methods!

17. Acknowledgment
– Alice den Hertog - KIT Biomedical Research
– Richard Anthony - KIT Biomedical Research
– Frank Fey – Center for Consepts in
Mechatronics, Nuenen
– Colin J. Ingham – MicroDISH, Houten
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18. Accelerating Rapid
Diagnostics
LumiByte B.V.
De Pinckart 24
Postbus 12
5670 AA Nuenen
Tel. +31 (0)88 1168600
www.lumibyte.eu
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