Presentation designed for laboratory staff with computers that would not display Flash. Used PowerPoint to create an eLearning module on how to operate the pH meter.

1. Measuring pH Using the Orion
3 Star Plus pH Meter with Printer
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 1

2. Orion 3 Star Plus pH Meter
Front of Meter & Keypad
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 2

3. Start Up
• Turn Printer ON
• Press POWER Key on
Meter
 Display will read:
 Star PLUS
 Software Version and
current method
 Header information will
print:
 Meter Serial Number
 Meter Software Version
 Method currently set
• Check print quality
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 3

4. Calibrate Method 1
Open
Fill Hole
Rinse electrode and
Select Method (#
blot carefully
decimals) to be used
for samples
Press MEASURE
to set method
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 4

5. Calibrate Method 1
CAL 1 Displayed..
Press CALIBRATE
• pH will stop flashing when
reading stable
• Buffer value will be displayed
Immerse electrode
with leading zeroes
in first buffer
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 5

6. Calibrate Method 1
After pressing CALIBRATE,
• CAL 2 displayed
• pH flashes while reading in
progress
• Remove electrode from
first buffer, rinse & blot
• Immerse electrode in
second buffer
• pH will stop flashing when
reading stable
• Buffer value will be displayed
with leading zeroes
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 6

7. Calibrate Method 1
After pressing CALIBRATE,• Remove electrode from
• CAL 3 displayed
pH will stop flashing when
second buffer, rinse & blot
• pH flashes while reading in
reading stable • Immerse electrode in third
• progress
Buffer value will be displayed
buffer
with leading zeroes
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 7

8. Completing Calibration
Press MEASURE to print
& save calibration results
• Check date and time
(not visible in photo)
• Check mV for each
buffer
• Check slope 1 and slope
2
• Initial & date printout
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 8

9. Verifying Instrument Performance
• Remove electrode from
buffer, rinse & blot
• Immerse electrode in QC
Press MEASURE (6.86) buffer
pH will stop flashing when
reading stable
Press MEASURE
to print result
• Initial & date
• Identify sample as
QC Buffer
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 9

10. Documenting Calibration
Calibration & Initial
QC Buffer on form
• Check Dates & Times
• Check Method
• Check Calibration #
• Check Limits:
• 4.01 +177 mV ± 30 mV
• 7.00 0 mV ± 30 mV
• 10.01 -177 mV ± 30 mV
• Slope 1 and 2:
95% - 102%
• QC Buffer: 6.86 ± 0.05
Method 1: 6.8 – 6.9
Method 2: 6.81 – 6.91
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 10

11. Measuring Sample pH
• Remove electrode from
buffer or storage solution,
rinse & blot
• Immerse electrode in
Press MEASURE sample
pH will stop flashing when
reading stable
Press MEASURE
again to print result
• Initial & date
• Identify sample
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 11

12. Reviewing Results
• Check Dates & Times
• If > 2 hours since last QC Buffer run,
instrument performance must be
verified by running the QC Buffer as a
Batch control
• Check Method
• 1 decimal for any product QC and most
reagents/solution
• 2 decimals if reagent procedure
requires more sensitivity
• NO ROUNDING
• Check Calibration #
• Match to the most recent calibration for
the method selected
• Attach product results to Product QC
Forms
• Attach reagent/solution pH
measurements to Reagent pH Log
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Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 12