Molecular mapping of genes for resistance to the bean pod weevil
                          (Apion godmani Wagner) in commo...
Upcoming SlideShare
Loading in...5
×

Poster62: Molecular mapping of genes for resistance to the bean pod weevil (Apion godmani Wagner) in common bean

528

Published on

Poster for CIAT 2009 Knowledge Sharing Week

Published in: Technology, Travel
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
528
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
8
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "Poster62: Molecular mapping of genes for resistance to the bean pod weevil (Apion godmani Wagner) in common bean"

  1. 1. Molecular mapping of genes for resistance to the bean pod weevil (Apion godmani Wagner) in common bean M.W. Blair1*, C. Muñoz1, R. Garza2, C. Cardona1 1/ CIAT - International Center for Tropical Agriculture, A. A. 6713, Cali, Colombia, 2/ INIFAP, A.P. No. 10, Chapingo, Estado de Mexico 56230, Mexico Justification Figures and Tables Results and Discussion The bean pod weevil (Apion godmani Wagner) is After cloning and conversion to sequence tagged Table 1. Percentage seed damage by Apion godmani in recombinant a serious insect pest of common beans inbred lines (RILs) and parents. site (STS) markers, one of the markers was (Phaseolus vulgaris L.) grown in Mexico and Genotype 1994 1995 1996 1997 polymorphic as a SCAR marker while four detected Central America that is best controlled by host- Mean 10 most resistant RILs 5.2b ± 0.57 6.7b ± 0.53 11.6c ± 0.84 12.5b ± 0.68 polymorphism as CAPS markers upon digestion with restriction enzymes (Figure 2). plant resistance available in Durango or Jalisco Mean 10 most suscep tible RILS s 38.0a ± 0.96 34.2a ± 1.08 42.0b ± 1.56 55.6a ± 1.60 genotypes such as J-117. Given unreliable ‘Jamapa’ (susc eptible parent) 44.8a ± 2.84 35.6a ± 3.19 56.3a ± 2.80 61.2a ± 2.78 Nine molecular markers for Apion resistance were infestation by the insect, the use of marker- J 117 (resistant parent) 1.1b ± 0.45 4.0b ± 0.74 9.1c ± 0.82 6.6b ± 1.31 mapped to loci on chromosomes 2, 3, 4 and 6 (linkage assisted selection is desirable. groups b01, b08, b07and b11,respectively) based on genetic analysis the Jamapa x J-117 population and two reference populations (DOR364 x G19833 and Introduction BAT93 x JaloEEP558) for which chromosome and linkage group designations are known (Figure 3). Apion godmani (Wagner) is a destructive insect pest of common beans grown in highland regions of Mexico The loci on linkage groups b01 and b11 loci may be (states of Puebla, Durango, and Chiapas) and Central associated with the Agr and Agm genes while America (especially Guatemala, Honduras, El Salvador additional resistance genes may exist on b07 and b08. and Northern Nicaragua). Insect life cycle alternates These are among the first specific markers between forest soils / crop refuse where the adults over- developed for tagging insect resistance in common winter and fields of common bean where they bean and are expected to be useful for evaluating the reproduce. Bean pod weevils generally appear in bean mechanism of resistance to A. godmani and for crops during flowering and consume small amounts of Figure 1. Population distribution for 50 recombinant inbred lines from the Jamapa x J-117 population over four seasons. marker assisted selection of resistance in breeding young leaves and flowers before beginning to programs for Mexico and Central America. reproduce. Damage occurs when the bean pod weevil females lay their eggs in the mesocarp of 1- 4 cm long b01 markers (I) b01 markers (II) developing common bean pods and the resulting larvae W9-1300S- OPF-10 F10-500S OPU-01 U1-1400R burrow into the immature seeds inside causing yield M 1 2 3 4 5 6 7 8 m bp M 1 2 3 4 m 5 6 7 8 bp M 1 OPW-09 2 3 4 m 5 W9-1300S 6 7 8 9 AluI 10 11 12 M bp 1 OPZ-04 2 3 4 M 5 Z4-800R 6 7 8 bp loss, reduced seed quality and lower seed viability. Yield loss caused by the insect is variable depending on the climatic conditions of the previouis year but can be 1400 1237 up to 90%. 460 800 500 650 Objectives 270 Given that major genes are thought to be involved in resistance, our objective in this study was to develop b08 markers b11 markers STS markers for Apion resistance using bulked C1-800S- B1-1400R- segregant analysis of recombinant inbred lines derived OPK -16 SK16 OPC-01 C1–800S RsaI OPW-06 SW6-800R OPB-01 B1-1400R TaqI bp M 1 2 3 4 5 7 M bp M 1 2 3 4 5 6 7 8 9 10 M bp bp M 1 2 3 4 5 6 7 8 M bp M 1 2 3 4 5 6 7 8 9 10 11 12 M bp from the cross Jamapa x J-117, where ‘J-117’ is a resistant landrace source and ‘Jamapa’ is a susceptible black-seeded cultivar from Mexico. In this study we 1400 developed a total of nine SCAR and CAPS markers, 890 732 780 confirmed their linkage to Apion resistance genes and 682 682 550 521 600 mapped them to their genomic position based on integrated genetic maps of common beans. 132 Materials and Methods Plant Material: Recombinant inbred line population (50 Figure 2. (above). SCAR and CAPS markers developed from genotypes) was developed from a cross of Jamapa (susceptible) polymorphic RAPD bands found in bulked segregant analysis of and J-117 (resistant) through single seed descent to the F5 Apion godmani resistant and susceptible parents and individuals generation and evaluated for Apion resistance over four of the Jamapa x J-117 population. SCAR. consecutive seasons (1994 – 1997) at the Santa Lucía de Prías Experiment Station (INIFAP) near Texcoco. Resistance was Figure 3. (left). Linkage relationships of Apion resistance RAPD, measured as damage level on a random sample of 30 pods per SCAR and CAPS markers in recombinant inbred line populations Jamapa x J-117 (JJ), DOR364 x G19833 (DG) and BAT93 x Jalo plot taken at crop maturity (Table 1). EEP 558 (BJ) for linkage groups b01, b07, b08 and b11. Bulked Segregant Analysis: DNAs from the four most resistant and four most susceptible recombinant inbred lines were combined into their respective bulks. A total of 131 decamer References primers were used to discover random amplified (RAPD) polymorphisms among the parents that were also observed in Blair MW, Muñoz C, Garza R, Cardona C (2006) Molecular mapping of genes for resistance to the bean pod weevil (Apion the resistant and susceptible bulks. Both repulsion and coupling godmani Wagner) in common bean. Theor Appl Genet 112: markers were considered as long as the presence or absence of 913–923. the bands was consistent between the bulks and the parents.

×