Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.

1. Bio.Kitchen
your lab for the Biological Transformation
“I think the biggest innovations of the 21st century will
be at the intersection of biology and technology. A new
era is beginning.” - Steve Jobs
“If I were a teenager today, I'd
be hacking biology.” - Bill Gates

3. DNA

4. Transcription and Translation

6. Digital Biology

7. 2021
171%
of biocapacity used
https://www.footprintnetwork.org/2017/06/27/earth-overshoot

8. SARS-CoV 2

10. Gene Code Sars-Cov-2 Nucleocapsid
1 atgtctgata atggacccca aaatcagcga aatgcacccc gcattacgtt tggtggaccc
61 tcagattcaa ctggcagtaa ccagaatgga gaacgcagtg gggcgcgatc aaaacaacgt
121 cggccccaag gtttacccaa taatactgcg tcttggttca ccgctctcac tcaacatggc
181 aaggaagacc ttaaattccc tcgaggacaa ggcgttccaa ttaacaccaa tagcagtcca
241 gatgaccaaa ttggctacta ccgaagagct accagacgaa ttcgtggtgg tgacggtaaa
301 atgaaagatc tcagtccaag atggtatttc tactacctag gaactgggcc agaagctgga
361 cttccctatg gtgctaacaa agacggcatc atatgggttg caactgaggg agccttgaat
421 acaccaaaag atcacattgg cacccgcaat cctgctaaca atgctgcaat cgtgctacaa
481 cttcctcaag gaacaacatt gccaaaaggc ttctacgcag aagggagcag aggcggcagt
541 caagcctctt ctcgttcctc atcacgtagt cgcaacagtt caagaaattc aactccaggc
601 agcagtaggg gaacttctcc tgctagaatg gctggcaatg gcggtgatgc tgctcttgct
661 ttgctgctgc ttgacagatt gaaccagctt gagagcaaaa tgtctggtaa aggccaacaa
721 caacaaggcc aaactgtcac taagaaatct gctgctgagg cttctaagaa gcctcggcaa
781 aaacgtactg ccactaaagc atacaatgta acacaagctt tcggcagacg tggtccagaa
841 caaacccaag gaaattttgg ggaccaggaa ctaatcagac aaggaactga ttacaaacat

11. Protein Code
Nucleocapsid
>YP_009724397.2 nucleocapsid phosphoprotein [Severe acute
respiratory syndrome coronavirus 2]
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRP
QGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGY
YRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDG
IIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAE
GSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGD
AALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKP
RQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH
WPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDP
NFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKK
QQTVTLLPAADLDDFSKQLQQSMSSADSTQA

14. https://nextstrain.org/ncov/global?animate=2019-12-06,2020-09-22,0,1,30000&d=tree,map&p=grid

15. Corona Virus Family
https://smw.ch/article/doi/smw.2020.20224

16. Digital Biology

19. Write
Read
Write
Read
Reading and Writing Life

20. Cloning Cycle - Bacteria Incubation

21. Spectrophotometer

23. Cloning Cycle - RNA / DNA Extraction

25. Cloning Cycle - PCR / Copying DNA

26. open QPCR

29. Cloning Cycle - Sequencing

30. DNA READING - Costs per Genome dropped at 5x Moore’s Law Since 2001
© National Human Genome Research Institute
MOORE‘S LAW
COST PER GENOME

31. DNA READING - Sequencing Machines
LEARN MORE YOUR DNA FOR €169 ONE MILLION PEOPLE DONE
© Novogene

32. DNA READING - USB Sequencing Device
HOW IT WORKS BUY FOR $1000
© Oxford Nanopore

33. Cloning Cycle - DNA Synthesis

34. DESIGNING DNA – Development Tools
OPEN EDITOR
© Genome Compiler

35. Printing DNA - Kilobaser
© Applied BioSystems

37. Cloning Cycle - Protein Extraction

38. GMO Production and purification of LAMP enzymes
Heterologous Production
● E. Coli Tuner Cells
● Shaker Incubator
Expressed Enzymes
● isothermal Polymerase
● Reverse Transcriptase
Purification
● His-Tag Affinity Chromatography
● Ion-Exchange Chromatography
Quality Control
● SDS Page
● UV-Vis Spectroscopy

39. Moore’s Law for Biology
Exponentially grow the number of experiments per scientist
Experiments
per day
10.000.000
1.000.000
100.000
1.000
100
2010 2015
2021

40. 40

41. Biodigital Teleportation

42. Literature
www.buyzero.de
Questions?
trojok@unternehmertum.d
e

43. Open Source
improved data availability, transparency and analysis
Wetware
Software Hardware
BIO
COMMONS