conference presentation circa 2001

1. Characterization of Myostatin in Brook Trout Steven Roberts, Rick Goetz USDA Grant # 2001-35206-10943 Marine Biological Laboratory - Woods Hole, MA Program in Scientific Aquaculture

3. Disrupted gene in mice resulting in an increase in skeletal muscle Lee, S-J and A.C. McPherron 1999 Curr Op Gen & Dev 9:604-607

4. Maccatrozzo et al. 2001 FEBS Letters 509:36-40

8. RT-PCR – b/m MSTN MALES FEMALES RM WM

9. RT-PCR – ov MSTN RED MUSCLE WHITE MUSCLE MALE FEMALE

10. Only ov MSTN was detected fertilization eyeing hatching yolk-sac resorption Knight AE 1963 Trans Am Fish Soc. 92:344

11. Protein 18 kDa 52 kDa R W MSTNAb 31 kDa LAP Mature

12. MSTNAb (50 kDa) MALES FEMALES RM WM

13. MSTNAb (14 kDa) MALES FEMALES RM WM

14. MSTNAb (14 kDa) RM WM MALES FEMALES

15. MALES FEMALES Ab1 R W R W 50 kDa 14 kDa 14 kDa 50 kDa

16. MALES FEMALES 13 kDa 50 kDa 50 kDa 14 kDa 14 kDa LAP Mature

17. MALES FEMALES 50 kDa 14 kDa 14 kDa 13 kDa LAP Mature 50 kDa

18. MALES FEMALES 13 kDa LAP Mature 50 kDa 14 kDa 14 kDa 50 kDa

19. MALES FEMALES 13 kDa LAP Mature 50 kDa 14 kDa 14 kDa 50 kDa

21. fertilization eyeing hatching yolk-sac resorption Highest Expression Protein Expression Knight AE 1963 Trans Am Fish Soc. 92:344 Ov Transcript Expression