HYPOXIA IMPACTS ON                                                        PACIFIC OYSTER                                  ...
Hypoxia• Dissolved oxygen               CROSS                             Anthropogenic  concentration <2mg/l           SE...
Dead Zone?                                      AQUATICChronicle/Ken Stevens :Michigan Department of Natural Resources Tho...
Plasticity of Gene Expression?                                               HYPOXIAhttp://en.wikipedia.org/wiki/File:Phen...
Secondary Ecological Impacts• Heat-shock proteins • Hsp70
Secondary Ecological Impacts• Heat-shock proteins • Hsp70• Hypoxia-Inducible Factors • HIF-1α
Secondary Ecological Impacts• Heat-shock proteins • Hsp70• Hypoxia-Inducible Factors • HIF-1α• Insulin-like Growth Factor ...
Hypoxia Exposure  mRNA gene expression                         High DO                 Low DO                             ...
Pacific OysterWhy?• Abundant• Lives in the  intertidal zone• Commercially  important
Experimental Design
Hsp70                                   1.8                                   1.6                                   1.4   ...
HIF-1α
hrIGF-1                                       2.5                                        2     Mean mRNA expression of hrI...
Summary1. Hsp70: General stress response, but lower levels in Low DOacclimated group2. HIF-1α: Lower levels in Low DO accl...
Importance                Adapt                Grow                SurviveCommercial                Conservation
FISH441: Oyster Hypoxia and acclimation
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FISH441: Oyster Hypoxia and acclimation

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Environmental Physiology (FISH441) group presentation of research project

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  • HALLEY:In terms on North American hypoxia is associated with areas such as the Gulf of Mexico and the Chesapeake Bay, however it does occur on the west coast.Tends to be episodic, occurring during the summer after stratification of the water column, preventing the mixing of the warmer nutrient rich upper layer with the cooler dense bottom layer and water exchange is limited. The organic matter that forms in the nutrient rich surface waters ultimately sink to the bottom and stars to decompose; this respiration requires oxygen thus resulting in hypoxia/anoxia bottom waters. As the summer progresses the vertical range of hypoxia tends to increase over the summer months.Human activities have also been linked to the increase in duration, extent and magnitude of hypoxic events.
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  • the ability of an organism to change its phenotype in response to changes in the environment
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  • FISH441: Oyster Hypoxia and acclimation

    1. 1. HYPOXIA IMPACTS ON PACIFIC OYSTER (CRASSOSTREA GIGAS) GENE EXPRESSION Abby Tillotson Halley Nelson Rizky Darmawanhttp://osufoundation.org/news/featurednews/current/moore/index.htm
    2. 2. Hypoxia• Dissolved oxygen CROSS Anthropogenic concentration <2mg/l SECTION contribution• Worldwide • Estuaries • Semi-enclosed seas• Commonly seasonal• Anthropogenic• Increase in duration, extent Thermo- or and magnitude Pycnocline LOW DO Diaz (2001); Diaz and Rosenberg (2008)
    3. 3. Dead Zone? AQUATICChronicle/Ken Stevens :Michigan Department of Natural Resources Thousandsof gizzard shad were found along the shore (Mona Lake)
    4. 4. Plasticity of Gene Expression? HYPOXIAhttp://en.wikipedia.org/wiki/File:Phenotypic_plasticity_diagram.svg
    5. 5. Secondary Ecological Impacts• Heat-shock proteins • Hsp70
    6. 6. Secondary Ecological Impacts• Heat-shock proteins • Hsp70• Hypoxia-Inducible Factors • HIF-1α
    7. 7. Secondary Ecological Impacts• Heat-shock proteins • Hsp70• Hypoxia-Inducible Factors • HIF-1α• Insulin-like Growth Factor • hrIGF-1 http://pubs.niaaa.nih.gov/publications/arh26-4/274-281.htm
    8. 8. Hypoxia Exposure mRNA gene expression High DO Low DO Acclimation
    9. 9. Pacific OysterWhy?• Abundant• Lives in the intertidal zone• Commercially important
    10. 10. Experimental Design
    11. 11. Hsp70 1.8 1.6 1.4 mean mRNA expression of Hsp70 1.2 1 0.8 0.6 0.4 0.2 0 High DO Control Low DO Acclimation
    12. 12. HIF-1α
    13. 13. hrIGF-1 2.5 2 Mean mRNA expression of hrIGF-1 1.5 1 0.5 0 High DO Control Low DO Acclimation
    14. 14. Summary1. Hsp70: General stress response, but lower levels in Low DOacclimated group2. HIF-1α: Lower levels in Low DO acclimated group3. hrIGF-1: High levels in Low DO acclimated group
    15. 15. Importance Adapt Grow SurviveCommercial Conservation

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