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Bay scallop genetic diversity
and population structure in
       Massachusetts

              Steven Roberts
       School of Aquatic and Fishery Sciences
             University of Washington
Outline

•Beginning
• Marker Development
• Populations / Enhancement
• Conclusions
NCBI Present Status
Library Characteristics

                              • ESTs = 7057
Individual clones sequenced
   D-hinge 671

                              • CoreNucleotides = 85
   Met 61
   Set 932
   Adductor 367
   Gonad 58
8% sequences <200 bp

                              • Contigs 845
Average length - 719 bp
970 singletons
217 contigs
                              • Singletons 2746
                              • Unique Seqs 3591
Enhancement
Population Differences


             Self-assignment
             Only Adults
              Random = 11%
              Chatham = 60%
Assessing Enhancement




private alleles
showing up post
enhancement
Summary
• Identify markers that effectively identify
  all potential parent populations
 • functional markers (SSR-ESTs, SNPs)
• Isolation coupled with life history can
  dramatically affect genetic diversity
• Temporal (yearly) variation is interesting
  and critical for understanding diversity

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NSA08 Roberts Scallop

  • 1. Bay scallop genetic diversity and population structure in Massachusetts Steven Roberts School of Aquatic and Fishery Sciences University of Washington
  • 2. Outline •Beginning • Marker Development • Populations / Enhancement • Conclusions
  • 3.
  • 4.
  • 5.
  • 6. NCBI Present Status Library Characteristics • ESTs = 7057 Individual clones sequenced D-hinge 671 • CoreNucleotides = 85 Met 61 Set 932 Adductor 367 Gonad 58 8% sequences <200 bp • Contigs 845 Average length - 719 bp 970 singletons 217 contigs • Singletons 2746 • Unique Seqs 3591
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20. Population Differences Self-assignment Only Adults Random = 11% Chatham = 60%
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 27. Summary • Identify markers that effectively identify all potential parent populations • functional markers (SSR-ESTs, SNPs) • Isolation coupled with life history can dramatically affect genetic diversity • Temporal (yearly) variation is interesting and critical for understanding diversity