Bay scallop genetic diversity
and population structure in
       Massachusetts

              Steven Roberts
       School...
Outline

•Beginning
• Marker Development
• Populations / Enhancement
• Conclusions
NCBI Present Status
Library Characteristics

                              • ESTs = 7057
Individual clones sequenced
   D-...
Enhancement
Population Differences


             Self-assignment
             Only Adults
              Random = 11%
              Ch...
Assessing Enhancement




private alleles
showing up post
enhancement
Summary
• Identify markers that effectively identify
  all potential parent populations
 • functional markers (SSR-ESTs, S...
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
NSA08 Roberts Scallop
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NSA08 Roberts Scallop

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NSA08 Roberts Scallop

  1. 1. Bay scallop genetic diversity and population structure in Massachusetts Steven Roberts School of Aquatic and Fishery Sciences University of Washington
  2. 2. Outline •Beginning • Marker Development • Populations / Enhancement • Conclusions
  3. 3. NCBI Present Status Library Characteristics • ESTs = 7057 Individual clones sequenced D-hinge 671 • CoreNucleotides = 85 Met 61 Set 932 Adductor 367 Gonad 58 8% sequences <200 bp • Contigs 845 Average length - 719 bp 970 singletons 217 contigs • Singletons 2746 • Unique Seqs 3591
  4. 4. Enhancement
  5. 5. Population Differences Self-assignment Only Adults Random = 11% Chatham = 60%
  6. 6. Assessing Enhancement private alleles showing up post enhancement
  7. 7. Summary • Identify markers that effectively identify all potential parent populations • functional markers (SSR-ESTs, SNPs) • Isolation coupled with life history can dramatically affect genetic diversity • Temporal (yearly) variation is interesting and critical for understanding diversity

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