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Use of irradiation in agriculture
Stan Ignatowicz
Warsaw Agricultural University
Warsaw, Poland
Radiation techniques in
agriculture: food irradiation
Benefits of food
irradiation
Benefits of food irradiation
Reduction of pathogenic microorganisms;
Decontamination;
Extension of shelf-life;
Disinfestation;
Parasite disinfection
Delay of physiological processes: inhibition
of sprouting.
(1) Reduction of pathogenic
microorganisms
Food-borne disease
Incidence of food-borne disease is
increasing.
Public awareness of the health threat
posed by pathogens in food.
Public awareness of the health threat posed by
pathogens in food.
polio virus
Food-borne disease
USA: food-borne disease:
76 million cases;
325,000 hospitalizations;
5,000 deaths each year (100 deaths
per week).
Salmonella, Listeria, Toxoplasma –
1500 deaths annually.
Salmonella
Shigella
Shigella
Food-borne pathogenic
microorganisms
Listeria
monocytogenes :
dairy products,
processed meat
and other foods
kept under
refrigeration.
Vibrio :raw
mollusks.
Salmonella,
Campylobacter jejuni
- poultry
Escherichia coli
0157:H7 – meat
and dairy products,
hamburger meat,
apple juice, water
Examples:
fresh poultry
carcasses obtained
with GMP:
2.5 kGy is suggested
to eliminate
Salmonella and
some others.
2x shelf-life at
<5oC.
frozen and died
egg products –
2.5 kGy:
inactivation of
microorganisms in
the non-
pasteurized egg
products.
Mechanically separated meat
(MSM)
Dose of 4 kGy is very effective for
reduction the spoilage
microorganisms in MSM (pork, beef,
poultry).
Irradiated MSM can be used as an
additive to sausage production and to
other meat products.
(2) Decontamination
Spices, herbs
Spices, herbs,
vegetable
seasonings – heavily
contaminated with
microorganisms.
Before they can be
safely incorporated
into food products,
the microbial load
should be reduced.
Spices, herbs
Ethylene oxide –
carcinogen.
Fumigation with
ethylene oxide banned
in EU in 1991.
Irradiation – a viable
alternative.
Spices, herbs
20 countries;
>60,000 tonnes in 1997.
USA: 4,500 tonnes in 1993,
30,000 tonnes in 1997.
Decontamination:
local herbal seasonings
5 kGy;
gamma rays, accelerated
electrons;
The treatment preserved
• basic sensory properties;
• quality.
• the microbial load reduced.
Radiation hygienization of animal
feed
poultry feed;
7 kGy – inhibition of formation of toxins by
Aspergillus and Fusarium fungi.
A. repens and F. sporotrichioides – most
radioresistant (65% survival of spores) at a 7 kGy
dose.
Radiation hygienization of animal
feed
fodder for carp;
5 kGy.
>90% survival of fish fed irradiated feeds.
No negative effects.
High fish yield: 3 tonnes per hectare,
control – only 1 tonne per hectare.
Radiation hygienization of animal
feed
animal carcases for fur animals
5-8 kGy;
decreased microbial contamination.
Irradiation + low temp. = extension of
shelf-life.
Post-production medium of fungi
Fungi
Post-production medium
Utility of post-production medium of
Pleurotus ostreatus as protein rich
fodder component for animals;
7-10 kGy – wheat straw of good
quality.
culturing the fungi;
protein rich fodder component.
(3) Extension of shelf-life
Example: strawberries
Strawberries are frequently spoiled by
Botrytis mold.
2-3 kGy; cold - 10oC
shelf-life up to 14 days.
Quality of fruits should be as good as
possible before the treatment.
Example: strawberries
Combined process:
radiation treatment with 2.5 kGy;
wrapping in plastic;
cold storage at 2-4oC
Control of mold; extension of shelf-life.
Example: rye bread
The effective dose
for shelf-life of
irradiated rye
bread – 1.5 kGy.
Shelf-life up to 30
days in ambient
temp. (control: few
days).
(4) Disinfestation
Disinfestation
(5) Parasite disinfestation
Parasite disinfestation
Trichinella spiralis;
pork meat;
live for 20 years in
cysts;
0.15 kGy.
Taenia solium, T.
saginata
0.15-0.5 kGy:
lost of infection
ability.
(6) Delay of physiological
processes: inhibition of
sprouting
Inhibition of onion sprouting
Onions for long-term storage by
irradiation.
50-60 Gy; treatment not later than 2
months after harvest.
Over 90% consumers accepted the method.
Inhibition of garlic sprouting
Garlic for long-term storage by irradiation.
150 Gy: 6 and 11 month storage in temp.
4oC-15oC.
Quality higher by 22-36%.
The score of consumer tests was 7 in 9
point hedonic scale.
Inhibition of potatoes sprouting
The optimal absorbed dose for sprout
inhibition of potatoes varies with
the varieties,
time of irradiation after harvest and
post irradiation storage temperature.
For many varieties - the dose 70-100 Gy.
Acceptance of irradiated potatoes – 90%.
Inhibition of potatoes sprouting
Side effects of potatoes
irradiation:
increase of darkening or
browning,
decrease of wound healing
ability,
increase of storage rot,
spoilage,
sweetening.
Delay of physiological processes:
mushrooms
The technology of fresh mushrooms
irradiation consists of the following stages:
preparation of the mushrooms,
packaging,
transportation to the irradiator,
treatment with ionizing radiation,
storage of irradiated mushrooms and
transport to the market.
Delay of physiological processes:
mushrooms
Irradiation with high energy
electrons of 10 MeV
retarded the processes of
mushroom growth and
ageing.
Benefits:
the caps closed,
no elongation of stems,
lowered down the
discoloration.
Delay of physiological processes:
mushrooms
The dose of 2 kGy was more effective
in the keeping quality of mushrooms
at 8-day storage period in comparison
with lower doses.
Delay of physiological processes
to slow down the ripening of
some fruits:
- bananas,
- mangoes,
- papayas
by irradiation at 0.25 to 1 kGy
dose.
Irradiate fruits before
ripening starts!
Food irradiation application
Low doses (up to 1 kGy)
Inhibition of
sprouting 0.05-0.15
potatoes, onions,
garlic, root ginger,
yam,
Insect
disinfestation
and parasite
disfection
0.15-0.5
cereals and pulses,
fresh and dried
fruits, dried fish and
meat, fresh pork
Delay of
physiological
processes (e.g.
ripening)
0.25-1.0
fresh fruits,
vegetables
Food irradiation application
Medium doses (1-10 kGy)
Extension of
shelf-life 1.0-3.0
fresh fish,
strawberries,
mushrooms
Elimination of
spoilageand
pathogenic
organisms
1.0-7.0
fresh and frozen
seafood,raw or
frozen poultry and
meat, etc.
Improving
technological
properties of
food
0.25-1.0
grapes (increasing
juice yield),
dehydrated
vegetables
Food irradiation application
High doses (10-50 kGy)
Industrial
sterilization (+
mild heat)
30-50
meat, poultry,
seafood, prepared
foods, sterilized
hospital diets
Elimination of
spoilage and
pathogenic
organisms
30-50
Spices, enzyme
preparations, natural
gum
Food irradiation in Poland
Food irradiation in Poland
Food irradiation has been studied for a
long time in Poland.
The investigations had begun at the end of
1950-ties in the Warsaw Agricultural
University.
In the following years the research was
extended to the other scientific centers.
Book on food irradiation
1963, W. Bednarczyk – „Food
Preservation by Ionizing Radiation”.
Food irradiation in Poland
Till the end of 1970-ties the investigations on
food irradiation were of the random nature.
The objectives involved the fundamental research
area of food science.
The JECF recommended in 1980 the general
approval of foods treated with the doses up to 10
kGy as nconditionally wholesome: the interest on
practical application of food irradiation was gained
in Poland.
Food irradiation in Poland
In 1986 the governmentl bodies decided to
recognize the possibilities of practical
application of radiation techniques in
agriculture.
Program:
Radiation preservation of food,
Radiation hygienisation of animal feed,
Pilot plants for food irradiation.
Experimental Plant for Food
Irradiation: Warszawa-Włochy
The operator of the plant is the Institute
of Nuclear Chemistry and Technology,
Warsaw.
The plant is located in old Russian fortress
chambres.
Additional buildings: technological hall and
laboratory were constructed.
Experimental Plant for Food
Irradiation: Warszawa-Włochy
In the plant two electron beam acceleratores
are located:
10 MeV, 1 kW, build at the Institute for
Nuclear Studies in Swierk;
10 MeV, 10 kW, made in USSR.
Multipurpose irradiation plant
Operator: Dept. of Nuclear Methods in
Agriculture, Univ. of Agriculture, Poznań.
60Co with the initial activity of 550,000 Ci
(Nordion International).
Dreams ....., should be in 1994.
List of irradiated food cleared for human
consumption in Poland
Product Purpose of irradiation Dose (kGy)
onions sprout inhibition 0.06
garlic sprout inhibition up to 0.15
spices decontamination up to 10
potatoes sprout inhibition up to 0.1
mushrooms shelf-life extention up to 2.5
Thank you for your attention!

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use of irradiation in agricultural science

  • 1. Use of irradiation in agriculture Stan Ignatowicz Warsaw Agricultural University Warsaw, Poland
  • 2. Radiation techniques in agriculture: food irradiation Benefits of food irradiation
  • 3. Benefits of food irradiation Reduction of pathogenic microorganisms; Decontamination; Extension of shelf-life; Disinfestation; Parasite disinfection Delay of physiological processes: inhibition of sprouting.
  • 4. (1) Reduction of pathogenic microorganisms
  • 5. Food-borne disease Incidence of food-borne disease is increasing. Public awareness of the health threat posed by pathogens in food.
  • 6. Public awareness of the health threat posed by pathogens in food. polio virus
  • 7. Food-borne disease USA: food-borne disease: 76 million cases; 325,000 hospitalizations; 5,000 deaths each year (100 deaths per week). Salmonella, Listeria, Toxoplasma – 1500 deaths annually.
  • 10. Food-borne pathogenic microorganisms Listeria monocytogenes : dairy products, processed meat and other foods kept under refrigeration. Vibrio :raw mollusks. Salmonella, Campylobacter jejuni - poultry Escherichia coli 0157:H7 – meat and dairy products, hamburger meat, apple juice, water
  • 11. Examples: fresh poultry carcasses obtained with GMP: 2.5 kGy is suggested to eliminate Salmonella and some others. 2x shelf-life at <5oC. frozen and died egg products – 2.5 kGy: inactivation of microorganisms in the non- pasteurized egg products.
  • 12. Mechanically separated meat (MSM) Dose of 4 kGy is very effective for reduction the spoilage microorganisms in MSM (pork, beef, poultry). Irradiated MSM can be used as an additive to sausage production and to other meat products.
  • 14. Spices, herbs Spices, herbs, vegetable seasonings – heavily contaminated with microorganisms. Before they can be safely incorporated into food products, the microbial load should be reduced.
  • 15. Spices, herbs Ethylene oxide – carcinogen. Fumigation with ethylene oxide banned in EU in 1991. Irradiation – a viable alternative.
  • 16. Spices, herbs 20 countries; >60,000 tonnes in 1997. USA: 4,500 tonnes in 1993, 30,000 tonnes in 1997.
  • 17. Decontamination: local herbal seasonings 5 kGy; gamma rays, accelerated electrons; The treatment preserved • basic sensory properties; • quality. • the microbial load reduced.
  • 18. Radiation hygienization of animal feed poultry feed; 7 kGy – inhibition of formation of toxins by Aspergillus and Fusarium fungi. A. repens and F. sporotrichioides – most radioresistant (65% survival of spores) at a 7 kGy dose.
  • 19. Radiation hygienization of animal feed fodder for carp; 5 kGy. >90% survival of fish fed irradiated feeds. No negative effects. High fish yield: 3 tonnes per hectare, control – only 1 tonne per hectare.
  • 20. Radiation hygienization of animal feed animal carcases for fur animals 5-8 kGy; decreased microbial contamination. Irradiation + low temp. = extension of shelf-life.
  • 22. Post-production medium Utility of post-production medium of Pleurotus ostreatus as protein rich fodder component for animals; 7-10 kGy – wheat straw of good quality. culturing the fungi; protein rich fodder component.
  • 23. (3) Extension of shelf-life
  • 24. Example: strawberries Strawberries are frequently spoiled by Botrytis mold. 2-3 kGy; cold - 10oC shelf-life up to 14 days. Quality of fruits should be as good as possible before the treatment.
  • 25. Example: strawberries Combined process: radiation treatment with 2.5 kGy; wrapping in plastic; cold storage at 2-4oC Control of mold; extension of shelf-life.
  • 26. Example: rye bread The effective dose for shelf-life of irradiated rye bread – 1.5 kGy. Shelf-life up to 30 days in ambient temp. (control: few days).
  • 30. Parasite disinfestation Trichinella spiralis; pork meat; live for 20 years in cysts; 0.15 kGy. Taenia solium, T. saginata 0.15-0.5 kGy: lost of infection ability.
  • 31. (6) Delay of physiological processes: inhibition of sprouting
  • 32. Inhibition of onion sprouting Onions for long-term storage by irradiation. 50-60 Gy; treatment not later than 2 months after harvest. Over 90% consumers accepted the method.
  • 33. Inhibition of garlic sprouting Garlic for long-term storage by irradiation. 150 Gy: 6 and 11 month storage in temp. 4oC-15oC. Quality higher by 22-36%. The score of consumer tests was 7 in 9 point hedonic scale.
  • 34. Inhibition of potatoes sprouting The optimal absorbed dose for sprout inhibition of potatoes varies with the varieties, time of irradiation after harvest and post irradiation storage temperature. For many varieties - the dose 70-100 Gy. Acceptance of irradiated potatoes – 90%.
  • 35. Inhibition of potatoes sprouting Side effects of potatoes irradiation: increase of darkening or browning, decrease of wound healing ability, increase of storage rot, spoilage, sweetening.
  • 36. Delay of physiological processes: mushrooms The technology of fresh mushrooms irradiation consists of the following stages: preparation of the mushrooms, packaging, transportation to the irradiator, treatment with ionizing radiation, storage of irradiated mushrooms and transport to the market.
  • 37. Delay of physiological processes: mushrooms Irradiation with high energy electrons of 10 MeV retarded the processes of mushroom growth and ageing. Benefits: the caps closed, no elongation of stems, lowered down the discoloration.
  • 38. Delay of physiological processes: mushrooms The dose of 2 kGy was more effective in the keeping quality of mushrooms at 8-day storage period in comparison with lower doses.
  • 39. Delay of physiological processes to slow down the ripening of some fruits: - bananas, - mangoes, - papayas by irradiation at 0.25 to 1 kGy dose. Irradiate fruits before ripening starts!
  • 40. Food irradiation application Low doses (up to 1 kGy) Inhibition of sprouting 0.05-0.15 potatoes, onions, garlic, root ginger, yam, Insect disinfestation and parasite disfection 0.15-0.5 cereals and pulses, fresh and dried fruits, dried fish and meat, fresh pork Delay of physiological processes (e.g. ripening) 0.25-1.0 fresh fruits, vegetables
  • 41. Food irradiation application Medium doses (1-10 kGy) Extension of shelf-life 1.0-3.0 fresh fish, strawberries, mushrooms Elimination of spoilageand pathogenic organisms 1.0-7.0 fresh and frozen seafood,raw or frozen poultry and meat, etc. Improving technological properties of food 0.25-1.0 grapes (increasing juice yield), dehydrated vegetables
  • 42. Food irradiation application High doses (10-50 kGy) Industrial sterilization (+ mild heat) 30-50 meat, poultry, seafood, prepared foods, sterilized hospital diets Elimination of spoilage and pathogenic organisms 30-50 Spices, enzyme preparations, natural gum
  • 44. Food irradiation in Poland Food irradiation has been studied for a long time in Poland. The investigations had begun at the end of 1950-ties in the Warsaw Agricultural University. In the following years the research was extended to the other scientific centers.
  • 45. Book on food irradiation 1963, W. Bednarczyk – „Food Preservation by Ionizing Radiation”.
  • 46. Food irradiation in Poland Till the end of 1970-ties the investigations on food irradiation were of the random nature. The objectives involved the fundamental research area of food science. The JECF recommended in 1980 the general approval of foods treated with the doses up to 10 kGy as nconditionally wholesome: the interest on practical application of food irradiation was gained in Poland.
  • 47. Food irradiation in Poland In 1986 the governmentl bodies decided to recognize the possibilities of practical application of radiation techniques in agriculture. Program: Radiation preservation of food, Radiation hygienisation of animal feed, Pilot plants for food irradiation.
  • 48. Experimental Plant for Food Irradiation: Warszawa-Włochy The operator of the plant is the Institute of Nuclear Chemistry and Technology, Warsaw. The plant is located in old Russian fortress chambres. Additional buildings: technological hall and laboratory were constructed.
  • 49. Experimental Plant for Food Irradiation: Warszawa-Włochy In the plant two electron beam acceleratores are located: 10 MeV, 1 kW, build at the Institute for Nuclear Studies in Swierk; 10 MeV, 10 kW, made in USSR.
  • 50. Multipurpose irradiation plant Operator: Dept. of Nuclear Methods in Agriculture, Univ. of Agriculture, Poznań. 60Co with the initial activity of 550,000 Ci (Nordion International). Dreams ....., should be in 1994.
  • 51. List of irradiated food cleared for human consumption in Poland Product Purpose of irradiation Dose (kGy) onions sprout inhibition 0.06 garlic sprout inhibition up to 0.15 spices decontamination up to 10 potatoes sprout inhibition up to 0.1 mushrooms shelf-life extention up to 2.5
  • 52. Thank you for your attention!