The document describes experiments testing the bacterial inhibiting and color fastness properties of chitosan and cotton. It involves growing two types of bacteria (Staphylococcus aureus and Escherichia coli) on plates containing samples and monitoring growth over 24 and 48 hours. It also involves dyeing cotton and cotton chitosan samples and testing their color fastness after washing. The results show that bacteria grew less densely on plates containing chitosan samples compared to cotton samples, indicating chitosan can decrease bacterial growth. It also shows the cotton chitosan had better color fastness than plain cotton.

1. Process of testing bacterial inhibiting property ( 1 ) Put bacteria on all plates Spread plate Put all plates into incubator at 37 o C for 24 hours Put a piece of cotton on each plate

2. Process of testing bacterial inhibiting property ( 2 ) Move every piece of cotton from the plate in incubator to another plate with media Put all plates into incubator at 37 o C for 24 hours put all plates into incubator at 37 oC for 24 hours

3. Result of testing Staphylococcus aureus bacterial inhibiting property

4. CONTROL 24 ชม . 48 ชม .

5. Fluka 24 ชม . 48 ชม .

6. Biofile 24 ชม . 48 ชม .

7. S 1 24 ชม . 48 ชม .

8. Fluka xanthate 24 ชม . 48 ชม .

9. Biofile xanthate 24 ชม . 48 ชม .

10. S 1 xanthate 24 ชม . 48 ชม .

11. Note : + Found less densely bacteria ++ Found medium densely bacteria +++ Found high densely bacteria ++ + S 1 X +++ ++ Biofile X ++ + Fluka X +++ +++ S 1 +++ ++ Biofile ++ + Fluka +++ +++ CONTROL Past to 48 hour Past to 24 hour Kind of Chitosan Staphylococcus aureus

12. Result of testing Escherichia coli bacterial inhibiting property

13. CONTROL 24 ชม . 48 ชม .

14. Fluka 24 ชม . 48 ชม .

15. Biofile 24 ชม . 48 ชม .

16. S 1 24 ชม . 48 ชม .

17. Fluka xanthate 24 ชม . 48 ชม .

18. Biofile xanthate 24 ชม . 48 ชม .

19. S 1 xanthate 24 ชม . 48 ชม .

20. Note : + Found less densely bacteria ++ Found medium densely bacteria +++ Found high densely bacteria +++ +++ S 1 X ++ ++ Biofile X ++ + Fluka X ++ ++ S 1 ++ + Biofile ++ + Fluka +++ +++ CONTROL Past to 48 hour Past to 24 hour Kind of Chitosan Escherichia coli

21. Process of testing the color fastness Dye the cotton for 1 hour by 50 cm 3 of the color solution , concentration 1% by m /v dry the cotton in the air W ash in th e distilled water.

22. C onclu sion 1. the cotton chitosan has a clearer pleat than the cotton.

23. 1. the bacteria in the cotton chitosan plate grow less densely than the bacteria in the cotton plate. It may be assumed that chitosan can decrease the growth of Staphylococcus aureus and Escherichia coli . C onclu sion

24. 3. the color fastness property of the cotton chitosan is better than that of the cotton. C onclu sion