Sonali.CV.PPT
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Sonali Uttam is currently pursuing an MSc in Experimental Medicine at McGill University. She received a BTech in Biotechnology from Amity University in 2015. For her undergraduate thesis, she studied the regulatory role of heat stress transcription factor A7a in response to high temperature stress in Arabidopsis thaliana. She has work experience from an industrial training program at Imperial Life Sciences and as a Junior Research Fellow at the Translational Health Science and Technology Institute studying bacterial sepsis in neonates. Her goals are to pursue biomedical research and develop tools to help doctors better treat and diagnose diseases.
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Sonali.CV.PPT
- 1. Sonali Uttam
- 2. Education • Msc. Experimental Medinice (Present Student) McGill University • Biotechnology (2016) McGill University • B.Tech.- Biotechnology (2011-15) Amity University
- 3. Bachelors of Technology in Biotechnology 2011-2015 Amity University, Noida, India Thesis: ‘To Study the regulatory role of heat stress transcription factor A7a in response to high temperature stress in Arabidopsis thaliana.’ Supervisor: Dr. Manu Agarwal BACHELOR EDUCATION
- 4. (a) (c)(b) BACHELOR THESIS Col-O mA7a HSF HSF HS HSP Heat stress HSE • Grow Arabidopsis • Apply heat stress •RNA Extraction + DNase treatment • cDNA • Real Time PCR • Fold Change (Δ Δ Ct method)
- 5. 0.00 50.00 100.00 150.00 200.00 250.00 300.00 350.00 400.00 450.00 Col-0 Control Col-0 6 h hsfa7a Control hsfa7a 6 h Test Gene 1 Test Gene 1 BACHELOR THESIS
- 6. Preadipocytes AdipocytesOil Red O Staining of Adipocytes Mammalian Cell Culture
- 7. EXPERIENCE Project trainee November, 2015 – February, 2016 Biotech Industrial Training program Conducted by: Biotech Consortium India Limited (BCIL) on behalf of Department of Biotechnology (DBT) Government of India. Posted at: Imperial Life Sciences, Gurgaon • Data Curation • Liquid Biopsy Panel • Microarray (Affymetrix) • MassARRAY (Sequenom)
- 8. EXPERIENCE Junior Research Fellow February – August, 2016 Vaccine and Infectious Disease Research Centre (VIDRC) Translational Health Science and Technology Institute (THSTI), India. Project: Bacterial Sepsis in Neonates Funded by: Department of Biotechnology, Govt. of India Principal Investigator: Dr. Krishnamohan Atmakuri •Isolation of bacteria from blood • RNA extraction – Blood, Bacteria • Protein isolation • SDS PAGE, Silver staining
- 9. 0 20 40 60 80 100 120 Day 0 Day 4 Day 7 %viability Storage period Recovery after storage on 4°C without HiSep Suggested MicrobEnrich Filtrationhypaque ficollCentrifugation
- 10. y = -3.3984x + 39.095 R² = 0.9935 20.00 25.00 30.00 35.00 40.00 0.00 2.00 4.00 6.00 CT Log of amount of cDNA (pg) Standard curve Ct value vs. amount of cDNA used in a qPCR reaction M. smeg gDNA 20 kb
- 11. What I want to do: • Pursue Biomedical research •Develop tools which can be used by doctors to treat / diagnose diseases better
- 12. Thank you!