The document describes the infrastructure for three zones - Atlanta, Houston, and Austin. It includes details on the servers, storage, and networking in each zone. The Atlanta zone has 10 servers with 1.1TB of SAN storage and 2 Oracle databases. It also has core servers, satellites, and infrastructure/slice components across two datacenters. Similarly, the Houston and Austin zones each have 8 satellite servers running on ESX virtual machines across two datacenters with various networking components.
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Bennington College was facing declining enrollment and rising costs in the 1990s. To address this, the admissions office was overhauled and a new recruitment strategy was developed focused on reimagining the student experience from initial contact through enrollment. This included redesigning marketing materials like viewbooks, the application, and campus visits to better convey Bennington's distinctive curriculum and invite students to engage in the type of work done there. The results were dramatic increases in applications, enrollment yields, and the academic quality of incoming classes without a significant budget increase.
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Nick Carraway begins his story by describing how it will end. "Gatsby turned out all right," he explains, "it is what preyed on Gatsby...that temporarily closed out my interest in the abortive sorrows and short-winded elations of men" (6-7). By insisting from his narrative's outset on its hero's happy ending, Nick sets for himself an essentially elegiac ambition: to ensure that his readers come to the last page of the novel convinced that Gatsby is "something gorgeous."
The document describes the infrastructure for three zones - Atlanta, Houston, and Austin. It includes details on the servers, storage, and networking in each zone. The Atlanta zone has 10 servers with 1.1TB of SAN storage and 2 Oracle databases. It also has core servers, satellites, and infrastructure/slice components across two datacenters. Similarly, the Houston and Austin zones each have 8 satellite servers running on ESX virtual machines across two datacenters with various networking components.
Re-imagining the College Application | ColemanDan Coleman
Bennington College was facing declining enrollment and rising costs in the 1990s. To address this, the admissions office was overhauled and a new recruitment strategy was developed focused on reimagining the student experience from initial contact through enrollment. This included redesigning marketing materials like viewbooks, the application, and campus visits to better convey Bennington's distinctive curriculum and invite students to engage in the type of work done there. The results were dramatic increases in applications, enrollment yields, and the academic quality of incoming classes without a significant budget increase.
Desire & the Elegiac Narrator: Building a World where Gatsby Turns Out All Ri...Dan Coleman
Nick Carraway begins his story by describing how it will end. "Gatsby turned out all right," he explains, "it is what preyed on Gatsby...that temporarily closed out my interest in the abortive sorrows and short-winded elations of men" (6-7). By insisting from his narrative's outset on its hero's happy ending, Nick sets for himself an essentially elegiac ambition: to ensure that his readers come to the last page of the novel convinced that Gatsby is "something gorgeous."
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Tất cả các loại thực phẩm chức năng có đầy đủ các chứng nhận đều tốt. Nhưng riêng Vision có "Những ưu thế vượt trội" so với các loại thực phẩm chức năng khác.
www.mientayvn.com Tải thêm các tài liệu sinh học khác tại địa chỉ:
https://drive.google.com/folderview?id=0Bw5sTGnTS7NhUk01a3RYQV9TUjJ4blJDUDcyekp6UQ&usp=sharing
www.mientayvn.com Tải thêm các tài liệu sinh học khác tại địa chỉ:
https://drive.google.com/folderview?id=0Bw5sTGnTS7NhUk01a3RYQV9TUjJ4blJDUDcyekp6UQ&usp=sharing
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www.mientayvn.com Tải thêm các tài liệu sinh học khác tại địa chỉ:
https://drive.google.com/folderview?id=0Bw5sTGnTS7NhUk01a3RYQV9TUjJ4blJDUDcyekp6UQ&usp=sharing
www.mientayvn.com Tải thêm các tài liệu sinh học khác tại địa chỉ:
https://drive.google.com/folderview?id=0Bw5sTGnTS7NhUk01a3RYQV9TUjJ4blJDUDcyekp6UQ&usp=sharing
18. 1.2. C¬ chÕ tÝch cùc:
a. §Æc ®iÓm
s¬ ®å minh ho¹ ®Æc ®iÓm ®iÒu hoμ qu¸ tr×nh tæng hîp
pr«tªin ë Procaryota theo c¬ chÕ tÝch cùc
GGeenn đđiiềềuu hhooàà((RR))
ADN
OOPPEERRÔÔNN
VVïïnngg cchhØØ hhuuyy ((OO))NNhhóómm ggeenn ccấấuu ttrrúúcc
AA BB CC
mARN
CCáácc pphhầầnn
ttửử hhoo¹tt hhoo¸
Qu¸ tr×nh tổng hợp mARN diÔn
ra
19. 1.2. C¬ chÕ tÝch cùc:
a. §Æc ®iÓm
+ Ho¹t ®éng cña gen chØ b¾t ®Çu khi cã mÆt cña mét lo¹i
protein ho¹t ho¸ (®¬n chÊt hay phøc chÊt). Khi chÊt ho¹t
ho¸ nμy t
¬ng t¸c víi vÞ trÝ ®Æc hiÖu trªn vïng ®iÒu khiÓn
(activator) th× qu¸ tr×nh phiªn m· x¶y ra.
b. VÝ dô1: Ho¹t ®éng cña operon ara theo c¬ chÕ
tÝch cùc
Quan s¸t h×nh vÏ: M« t¶ cÊu tróc, ®Æc ®iÓm vμ c¬ chÕ ho¹t
®éng cña operon ara.
20.
21. + CÊu tróc, ho¹t ®éng cña Operon ara:
- CÊu tróc: Gen A m· cho izomerase
Operon aza gåm Gen B m· ho¸ cho kinase
Gen D m· ho¸ cho epimerase
S¶n phÈm cña c¸c gen nμy cÇn cho q/t biÕn ®æi ®êng
ara thμnh xelluloz –
5- photphat
22. + §Æc ®iÓm ho¹t
-®Khéin mg«: i trêng
-Khi m«i trêng
kh«ng cã arabinonse th× E trong TB rÊt Ýt
hÕt arabinonse th× E l¹i gi¶m nhanh
-Khi m«i trêng
cã bæ sung arabinonse th× sè lîng
E t¹o ra
trong TB t¨ng nhanh
30. 1.4.Ho¹t ho¸ ph¶n håi:
a. §Æc ®iÓm: s¶n phÈm do gen (operon) ®ã t¹o ra quay trë
l¹i ho¹t ho¸ gen (operon) ®ã.
b. VÝ dô: Sù ho¹t ®éng cña gen ara C
• C¸c c¬ chÕ kh¸c:
• VÝ dô: §iÒu khiÓn gen th«ng qua c¸c promotor kh¸c
•
PG2 PG2 +
mARN
+
35. CÊu tróc vμ chøc n¨ng cña nhãm gen m· ho¸ c¸c enzym cu¶
chu tr×nh tiÒm tan hoÆc sinh tan:.
36. CÊu tróc cña nhãm gen m· ho¸ c¸c enzym cña chu tr×nh tiÒm
tan hoÆc sinh tan:
P PR Cro CII E ORN PL OL1 OL3 PRM 2 OR1 ORCIII OL2 CI 3
Trong ®ã: Gen CIII ® Pro CIII ® ®/h ho¹t ®éng gen Cro,
CII.
Gen N ® Pro N ® ng¨n c¶n dõng p/tæng
hîp.
Gen CI ® Pro CI ® ®/h ho¹t ®éng gen phÝa bªn
ph¶i vμ chÝnh nã (b¸m vμo vÞ trÝ O1, O2 ho¹t ho¸ PRM; khi
b¸m vμo c¶ O3 k×m h·m PRM
C r o Gen Cro ® Pro Cro ® ®/h ho¹t ®éng gen CI.
Gen CII ® Pro CII ® ®/h ho¹t ®éng cña gen CI
PL, OL vïng ®iÒu kiÓn cña gen bªn tr¸i CI; PR, OR vïng ®iÒu
kiÓn cña gen bªn ph¶i CI ;PE , PRM vïng khëi ®éng cña gen
CI
46. 2.3.C¬ chÕ:
Khi yÕu tè Trans g¾n lªn vïng Cis ( b»ng liªn
kÕt yÕu h×nh thμnh gi÷a a.a cña Trans víi
baz¬ cña Cis) sÏ t¹o nªn chç gÊp cong cña ADN
Gãc gÊp cong cña ADN thay ®æi tïy theo lo¹i
protein vμ cã ¶nh hëng
®Õn tèc ®é ho¹t ®éng
cña gen.
§ång thêi Trans qua vïng ho¹t tÝnh thø 3 sÏ liªn
kÕt víi ARNpolymerase ë vïng promotor cña
gen ®Ó t¹o nªn nh÷ng vßng bªn
Sù h× nh thμ nh vß ng bªn t¹ o ® iÒ u kiÖn cho s ù
® /h phiªn m · bë i c ¸ c vïng ADN ë c ¸ ch x a nha u.
47. Trans 1 mÆt g¾n vμo Cis ë ®Çu 5’ cña gen ®Ých,
mÆt kh¸c sÏ t
¬ng t¸c víi nh÷ng protein ®/h kh¸c.
T
¬ng t¸c gi÷a protein ®/h vμ Trans cïng víi sù gÊp l¹i
cña ADN sÏ t¹o nªn 1 cÊu tróc thuËn lîi cho sù g¾n
enzym vμo promotor cña gen ®Ých . Lóc ®ã sù
phiªn m· cña gen ®Ých b¾t ®Çu
50. Ngoμi ra cßn cã c¬ chÕ ®/h b»ng chän läc
promotor
VÝ dô: Gen α-amylase, cã 2
promotor
Gen nμy m· hãa cho enzym α-amylase, gen
nμy cã 2 promotor: P1,P2; lμ gen kh¶m: chøa
c¸c Intron vμ c¸c Exon: S, L, 1,2,…
51.
52.
53. 3. §iÒu hßa ho¹t ®éng cña gen sau phiªn m·:
Bao gåm:
GhÐp nèi kh¸c biÖt VÝ dô: Gen calcitonine
63. 1.1.S÷a ch÷a b»ng c¸ch lo¹i bá c¸c
Baz¬( BER)
U
( sai háng hoÆc kh«ng thÝch hîp)
ADN
glycosylase
GAP endonuclease
ß
G
X
ADNpolymeras ß
e
Lygase
G
ß
G
U
-ADNglycosylase ph¸
hñy LK N-glycozit
HiÖn nay ®· biÕt ®îc
6 lo¹i
ADNglycosylase
-Ap endonuclease
c¾t LK
photphodieste ë vÞ
trÝ 5 ®Ó lo¹i bá ®ê-
nAgDNpolymerase
tæng hîp bæ sung
-Lygase nèi liÒn vÕt
hë
Gåm c¸c Enzym nhËn biÕt vμ c¾t bá c¸c baz¬ bÞ khö amin, c¸c baz¬ cã vßng
liªn kÕt bÞ hë hay cã liªn kÕt ®«i C=C®C-C.
64. 2. S÷a ch÷a b»ng c¸ch lo¹i bá c¸c nucleotid (NER
) Kh¸c víi c¬ chÕ BER
- Cho phÐp lo¹i bá vμ s÷a ch÷a bÊt kú sai háng nμo
c -ñCaÇ AnD sNù tham gia cña nhiÒu enzym ®Ó kiÓm
so¸t ADN
VÝ
ë vi khuÈn
dô:
Cã 4 enzym liªn quan ®Õn c¬ chÕ s÷a ch÷a NER
UvrA, UvrB, UvrC, UvrD
65. VÝ dô: ë vi khuÈn
UvrA + UvrB
ß
ß
Cã 4 enzym liªn quan ®Õn c¬
chÕ s÷a ch÷a NER
UvrA, UvrB, UvrC, UvrD
-UvrA+ UvrB® P/hîp, nhËn
biÕt vÞ trÝ sai háng vμ g¾n
-lUªnvr A® tã¸c h khái p/hîp nhê NL ATP
-UvrC + UvrB® P/hîp, cã ho¹t tÝnh
endonuclease, UvrB c¾t vÒ phÝa 3’
c¸ ch vÞ trÝ sai háng kho¶ng 4 nu, UvrC
c¾t vÒ phÝa 5’ c¸ch vÞ trÝ sai háng
-kUhov¶rnDg 8c nãu .ho¹t tÝnh Helicase,
lo¹i bá ®o¹n sîi ®¬n ADN
-ADNpolymerase tæng hîp bæ
s-Luynggase nèi liÒn vÕt hë
UvrA UvrC
ß ß
ß UvrD
ADNpolymerase ß Lygase
66. Phøc endonucleaseABC cïng phèi hîp víi 1 sè
protein m· bëi gen thuéc c¬ chÕ SOS, cã kh¶
n¨ng c¾t vμ thay thÕ ®o¹n ADN chøa lçi dμi
12®2000 nucleotid
C¬ chÕ s÷a ch÷a NER ë sinh vËt Eucaryota
x¶y ra t
¬ng tù nhë
vi khuÈn.
§o¹n ADN bÞ c¾t thêng
dμi 30
RnuÊctl ehoiÕtidm trêng
hîp ®¹t ®Õn
1500nucleotid