Tangential Flow Filtration (TFF) is a separation process that uses membranes to separate components in a liquid solution or suspension on the basis of size or molecular weight differences. Pellicon® cassettes combine the advantages of efficient, gentle processing, and linear scalability for effective, predictable scale-up from laboratory to process applications.

1. Scale-Up Quickly and Reliably
With Pellicon®
Tangential Flow Filtration Cassettes and Hardware
Introduction
Tangential Flow Filtration (TFF) is a separation process that uses membranes to separate
components in a liquid solution or suspension on the basis of size or molecular weight differences.
Pellicon®
cassettes combine the advantages of efficient, gentle processing, and linear scalability for effective, predictable scale-up from laboratory to
process applications.
Whether you are clarifying, concentrating, or purifying milliliters or thousands of liters of solution, you can have confidence in the Pellicon®
product
family of TFF cassettes to take you from bench-top to process volumes with predictable, scalable results.
The rugged design and chemical compatibility of Pellicon®
cassettes makes it the most reliable and best performing ultrafiltration system available.
The Pellicon®
cassette technology is available in a range of surface areas, for process development to purifying large volumes in pilot or manufacturing
scale. The membranes and feed channels stay the same, ensuring an easy-to-use, scale-up or scale-down transition from one surface area to the next.
Conclusion
The devices met or exceeded the acceptance criteria for comparability and scalability in the following areas:
Pellicon®
3 devices demonstrated similar performance despite containing different lots of membrane.
With Pellicon®
cassettes and hardware you can achieve:
Pellicon®
3 cassettes: mab & recombinant
protein, plasma
Ultracel®
PLC membrane: 10, 30 kD
Biomax®
membrane: 10, 30, 50 kD
Pellicon®
2 cassettes: All applications
Ultracel®
PLC membrane: 5, 10, 30, 100, 300, 1000 kD
Biomax®
membrane: 5, 8, 10, 30, 50, 100, 300, 500,
1000 kD
Durapore®
membrane: 0,1 0,22 0,45 0,65 um
Holder Family
A complete range of holders from pre-clinical up to full-scale manufacturing –
Stand alone or for integration into a system
TFF System Family
A complete range of easy-to-use, linearly scalable TFF systems
0.5 to 30 m20.1 to 1.5 m2
50 to 264 cm2
50 cm2
Cogent®
Process-Scale
System
Mobius®
FlexReady
Solution for TFF
Cogent®
M1
System
Cogent®
μScale
System
• More cost-effective feedstream
• Increase the speed to market by reducing scale-up testing
• Easier, more reliable scale-up and scale-down
• Higher product recovery
• Higher flux
• Higher yields
• Faster and gentler processing
• Greater robustness
Because of their reputation for performance and reliability, Pellicon®
cassettes with membranes have been utilized in almost every plasma, vaccine and
biotech protein production process in the world.
PRE-CLINICAL
PRE-CLINICAL
PHASE 2
PHASE 2
PHASE 3
PHASE 3
MARKETING APPROVALPHASE 1
PHASE 1
88 to 264 cm2 0.1 to 5 m2
5 to 140 m2
Pellicon®
2 Mini 0.1 m2
Pellicon®
2 Maxi 2.5 m2
Pellicon®
2 0.5 m2
300 ML – 100 L 2 L AND UP 10 L AND UP
Pellicon®
3 88 cm2
Pellicon®
3 0.11 m2
Pellicon®
3 0.57 m2
Pellicon®
3 1.14 m2
PILOT / PRODUCTIONSCALE-UP / SCALE-DOWN
Pellicon®
Cassettes
The ideal solution for your Tangential Flow Ultra Micro Filtration applications
0.0
0.0 10.0 20.0 40.0
20.0
60.0
80.0
TMP (psi)
Flux(lmh)
40.0
100.0
30.0 50.0
120.0
cassette 0.57m²cassette 88cm
cassette 0.11m² cassette 1.14m²
140.0
160.0
60.0 80.070.0 90.0
average +/-10%
average
Protein Process Performance in Scalability
The Pellicon®
lines of Ultracel®
TFF cassettes are used in a variety of applications within the biopharmaceutical industry. Devices containing 10kD
PLCGC membrane were selected as representative of the product family. These devices were challenged with a model protein stream to compare the
performance of the Pellicon®
3 minis as well as scalability within the Pellicon®
3 line.
The Pellicon®
3 cassettes met the
specifications for equivalency in terms of
protein flux performance. The average
limiting flux of the Pellicon®
88 cm2
cassette
fell within 10%.
Pellicon®
cassettes’ true linear scalability
allows for faster, but gentler processing
of proteins. This results in higher product
yields and a more cost-effective solution
for your purification and separation needs.
Figure 1: Average flux performance Pellicon®
3 cassettes with 40 g/L BSA
The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.
Copyright © 2017 Merck KGaA. All Rights Reserved. Merck, Millipore and the Vibrant M are trademarks of Merck. Pellicon, Ultracel, Biomax Durapore,
Mobius and Cogent are registered trademarks of Merck.
Lit. No. PS2299EN00 Ver. 2.0 | 2017 - 03030 | 03/2017
Frédéric SENGLER, Karine PERON, Millipore S.A.S.
2017 - 03030 - PS2299EN00 Scale up quickly and Reliably_V3.indd 1 3/27/17 9:19 AM