Sanger sequencing uses which of the following to determine the nucleotide sequence by terminating replication? Primers dNTPS ATP ddNTPs The insulin produced by recombinant DNA technology is: A combination of E . coli and human insulin Cheaper but less effective than pig insulin for treating diabetes Engineered to be more effective than human insulin Identical to human insulin produced in the pancreas Question 9 1 pts The PCR step during which the double- stranded template molecule becomes single-stranded is called Annealing Elongation Extension Denaturation.