Download to read offline
![Eckdaten
Gründung oyen.de
2001
Schwerpunkt als Berater, Trainer & Medienprogrammierer
Lösungsfindung für Publishing Herausforderungen
Schwerpunkte als Autor
Berichterstattung über Trends, Software und Technologien die
das systemgestützte Publizieren in die Ausgabekanäle Print,
Web und mobile Endgeräte beeinflussen [Multi Channel
Publishing]
0/3
KW So. 26/08/12](https://image.slidesharecdn.com/redaktionssysteme-2011-oyendekopie-120826091153-phpapp01/85/Redaktionssysteme-2011-3-320.jpg)
![Cover / Umschlag
Cover Vorwort Faxanfor
derung
Rücken
01 03
Rückseite Rückseite CMS
Smile
Publishing System
02 04
Publications
Heiler Print
Vorlaufseiten (Grau I) römisch nummeriert P ein Produkt pro Seite
6
LightCycler 480 System
Instrument 6
LightCycler 480 System
Instrument
Manager
Manager
Product 4711 Cat. No. Pack Size Price Product 4711 Cat. No. Pack Size Price
03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50
(96 reactions) (96 reactions)
Application Application
03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50
The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions)
to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50
DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions)
CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50
into vectors allows rapid protein expression from wild-type or modified (96 reactions) into vectors allows rapid protein expression from wild-type or modified (96 reactions)
DNA sequences. This method of generating DNA templates for protein ex- DNA sequences. This method of generating DNA templates for protein ex-
pression avoids the time-consuming step of cloning into expression plasmids Contents pression avoids the time-consuming step of cloning into expression plasmids Contents
and can be used for initial, rapid screening for expression. 1. Forward Primer and can be used for initial, rapid screening for expression. 1. Forward Primer
01 02 03 04
2. Reverse Primer 2. Reverse Primer
Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression
■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression
■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade
■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Benefit by adding epitope-tag sequences for detection with antibodies.
■ Flexibly introduce desired mutations, change codon usage and generate ■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins. truncated proteins.
Product Description Product Description
In order to generate linear expression constructs, two PCRs must be per- In order to generate linear expression constructs, two PCRs must be per-
formed. In the first reaction, gene-specific primers are used to add overlap formed. In the first reaction, gene-specific primers are used to add overlap
regions to the sequence of the target gene. The product of the first PCR is regions to the sequence of the target gene. The product of the first PCR is
mixed with two flanking primers and DNA fragments coding for the T7 pro- mixed with two flanking primers and DNA fragments coding for the T7 pro-
motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence
(C-terminal or N-terminal). In the second overlap extension PCR, the prod- (C-terminal or N-terminal). In the second overlap extension PCR, the prod-
uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’
ends are extended. This linear expression construct is then amplified via the ends are extended. This linear expression construct is then amplified via the
flanking primers. Figure 1: Principle of the generation of PCR products for in flanking primers. Figure 1: Principle of the generation of PCR products for in
vitro expression using RTS Linear Template Generation Sets. vitro expression using RTS Linear Template Generation Sets.
product TOC all List of
Real-Time PCR Systems
Real-Time PCR Systems
The introduction of an N-terminal His6-tag is shown. For detailed The introduction of an N-terminal His6-tag is shown. For detailed
Background Information Background Information
information on the mechanism, refer to the RTS website (www. information on the mechanism, refer to the RTS website (www.
One of the major advantages of cell-free compared to cell-based expression One of the major advantages of cell-free compared to cell-based expression
proteinexpression.com → RTS Wheat Germ Products → Linear proteinexpression.com → RTS Wheat Germ Products → Linear
systems is the ability to use PCR-generated templates. Using the RTS Wheat systems is the ability to use PCR-generated templates. Using the RTS Wheat
new
Templates and Vectors for both N- and C-terminal tagging). Templates and Vectors for both N- and C-terminal tagging).
Germ Linear Template Generation Set, His6-tag, if primers are designed for Germ Linear Template Generation Set, His6-tag, if primers are designed for
the first (gene specific) of the two PCRsaccording to the guidelines provided the first (gene specific) of the two PCRsaccording to the guidelines provided
in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products
duce a completely functional linear expression template for expression in Product 5088, see page 123 duce a completely functional linear expression template for expression in Product 5088, see page 123
either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-
tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ
Linear Template Generation Set). Primers for the second PCR are provided Linear Template Generation Set). Primers for the second PCR are provided
literature chapters Intern.
in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-
sign of these kits enables easy transfer of products from any expression sys- sign of these kits enables easy transfer of products from any expression sys-
Real-Time PCR
Real-Time PCR
tem to both the RTS Wheat Germ and E. coli platforms, as well as between tem to both the RTS Wheat Germ and E. coli platforms, as well as between
the two RTS systems. At a later step in the RTS workflow, insertion of these the two RTS systems. At a later step in the RTS workflow, insertion of these
products into an RTS pIVEX Wheat Germ vector may be required (e.g., to products into an RTS pIVEX Wheat Germ vector may be required (e.g., to
products
produce a template for expression scale-up to RTS 500 Wheat Germ CECF, produce a template for expression scale-up to RTS 500 Wheat Germ CECF,
or to ensure that a well-characterized [sequenced] and stabilized template is or to ensure that a well-characterized [sequenced] and stabilized template is
used in a series of subsequent experiments). used in a series of subsequent experiments).
& support Represent 103
Ordering and product information, or technical support:
www.pharma-enhance-science.com
Subscriptions for electronic Pharmaica, product spotlights and more:
www.pharma-enhance-science.com/enews
Information on products available in bulk: scbio.pharma.com
Bestellungen
Gebührenfrei: 0800/235-3152
Fax: 0621/235-3136
Online Shop: www.IBuyPharma.de
103
Ordering and product information, or technical support:
www.pharma-enhance-science.com
Subscriptions for electronic Pharmaica, product spotlights and more:
www.pharma-enhance-science.com/enews
Information on products available in bulk: scbio.pharma.com
Bestellungen
Gebührenfrei: 0800/235-3152
Fax: 0621/235-3136
Online Shop: www.IBuyPharma.de
material
01
P Produkt über 2 Seiten
LightCycler 480 System LightCycler 480 System
6 Instrument 6 Instrument
Product 4711 Cat. No. Pack Size Price Product 4711 Cat. No. Pack Size Price
03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50
(96 reactions) (96 reactions)
Application Application
03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50
The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions)
to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50
DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions)
CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50
into vectors allows rapid protein expression from wild-type or modified (96 reactions) into vectors allows rapid protein expression from wild-type or modified (96 reactions)
DNA sequences. This method of generating DNA templates for protein ex- DNA sequences. This method of generating DNA templates for protein ex-
pression avoids the time-consuming step of cloning into expression plasmids Contents pression avoids the time-consuming step of cloning into expression plasmids Contents
and can be used for initial, rapid screening for expression. 1. Forward Primer and can be used for initial, rapid screening for expression. 1. Forward Primer
2. Reverse Primer 2. Reverse Primer
Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression
■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression
■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade
■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Benefit by adding epitope-tag sequences for detection with antibodies.
■ Flexibly introduce desired mutations, change codon usage and generate ■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins. truncated proteins.
Product Description Product Description
In order to generate linear expression constructs, two PCRs must be per- In order to generate linear expression constructs, two PCRs must be per-
formed. In the first reaction, gene-specific primers are used to add overlap formed. In the first reaction, gene-specific primers are used to add overlap
regions to the sequence of the target gene. The product of the first PCR is regions to the sequence of the target gene. The product of the first PCR is
mixed with two flanking primers and DNA fragments coding for the T7 pro- mixed with two flanking primers and DNA fragments coding for the T7 pro-
Chapter 1-6
motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence
(C-terminal or N-terminal). In the second overlap extension PCR, the prod- (C-terminal or N-terminal). In the second overlap extension PCR, the prod-
uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’
ends are extended. This linear expression construct is then amplified via the ends are extended. This linear expression construct is then amplified via the
flanking primers. Figure 1: Principle of the generation of PCR products for in flanking primers. Figure 1: Principle of the generation of PCR products for in
vitro expression using RTS Linear Template Generation Sets. vitro expression using RTS Linear Template Generation Sets.
Real-Time PCR Systems
Real-Time PCR Systems
The introduction of an N-terminal His6-tag is shown. For detailed The introduction of an N-terminal His6-tag is shown. For detailed
Background Information Background Information
information on the mechanism, refer to the RTS website (www. information on the mechanism, refer to the RTS website (www.
One of the major advantages of cell-free compared to cell-based expression One of the major advantages of cell-free compared to cell-based expression
proteinexpression.com → RTS Wheat Germ Products → Linear proteinexpression.com → RTS Wheat Germ Products → Linear
systems is the ability to use PCR-generated templates. Using the RTS Wheat systems is the ability to use PCR-generated templates. Using the RTS Wheat
Templates and Vectors for both N- and C-terminal tagging). Templates and Vectors for both N- and C-terminal tagging).
Germ Linear Template Generation Set, His6-tag, if primers are designed for Germ Linear Template Generation Set, His6-tag, if primers are designed for
the first (gene specific) of the two PCRsaccording to the guidelines provided the first (gene specific) of the two PCRsaccording to the guidelines provided
in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products
duce a completely functional linear expression template for expression in Product 5088, see page 123 duce a completely functional linear expression template for expression in Product 5088, see page 123
either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-
tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ
Linear Template Generation Set). Primers for the second PCR are provided Linear Template Generation Set). Primers for the second PCR are provided
in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-
sign of these kits enables easy transfer of products from any expression sys- sign of these kits enables easy transfer of products from any expression sys-
Real-Time PCR
Real-Time PCR
01 02 03 04
tem to both the RTS Wheat Germ and E. coli platforms, as well as between tem to both the RTS Wheat Germ and E. coli platforms, as well as between
the two RTS systems. At a later step in the RTS workflow, insertion of these the two RTS systems. At a later step in the RTS workflow, insertion of these
products into an RTS pIVEX Wheat Germ vector may be required (e.g., to products into an RTS pIVEX Wheat Germ vector may be required (e.g., to
produce a template for expression scale-up to RTS 500 Wheat Germ CECF, produce a template for expression scale-up to RTS 500 Wheat Germ CECF,
or to ensure that a well-characterized [sequenced] and stabilized template is or to ensure that a well-characterized [sequenced] and stabilized template is
used in a series of subsequent experiments). used in a series of subsequent experiments).
Chapter 103
Ordering and product information, or technical support:
www.pharma-enhance-science.com
Subscriptions for electronic Pharmaica, product spotlights and more:
www.pharma-enhance-science.com/enews
Information on products available in bulk: scbio.pharma.com
Bestellungen
Gebührenfrei: 0800/235-3152
Fax: 0621/235-3136
Online Shop: www.IBuyPharma.de
103
Ordering and product information, or technical support:
www.pharma-enhance-science.com
Subscriptions for electronic Pharmaica, product spotlights and more:
www.pharma-enhance-science.com/enews
Information on products available in bulk: scbio.pharma.com
Bestellungen
Gebührenfrei: 0800/235-3152
Fax: 0621/235-3136
Online Shop: www.IBuyPharma.de
Num & Section Product Sonder
Section Intro pages Seiten 02
index (TOC (4 Vari.)
small)
P mehre Produkte pro Seite
Restriction Enzymes Restriction Enzymes
4 4
Asp EI Asp EI
from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price
11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50
(10 U/µl) (10 U/µl)
Compatible Ends: None. Compatible Ends: None.
Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents
Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B
Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC
Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer
with GC-RICH Solution: 100%. #not available in U.S. A B L M H with GC-RICH Solution: 100%. #not available in U.S. A B L M H
Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
Isoschizomers: Tth111 I. Isoschizomers: Tth111 I.
Heat inactivation: No information available. Heat inactivation: No information available.
Asp EI Asp EI
from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price
11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50
(10 U/µl) (10 U/µl)
Compatible Ends: None. Compatible Ends: None.
Mapping and Cloning of Nucleic Acids
Mapping and Cloning of Nucleic Acids
Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents
Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B
Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC
Temperature Optimum: +37°C Buffer Temperature Optimum: +37°C Buffer
Isoschizomers: Tth111 I. A B L M H Isoschizomers: Tth111 I. A B L M H
Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
Asp EI Asp EI
from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price
11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50
(10 U/µl) (10 U/µl)
Compatible Ends: None. Compatible Ends: None.
Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents
Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B
Molecular Biology
Molecular Biology
Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC
Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer
with GC-RICH Solution: 100%. #not available in U.S. A B L M H with GC-RICH Solution: 100%. #not available in U.S. A B L M H
Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
Isoschizomers: Tth111 I. Isoschizomers: Tth111 I.
Heat inactivation: No information available. Heat inactivation: No information available.
Asp EI Asp EI
from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price
11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50
(10 U/µl) (10 U/µl)
Compatible Ends: None. Compatible Ends: None.
Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents
Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B
Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC
Temperature Optimum: +37°C Buffer Temperature Optimum: +37°C Buffer
Isoschizomers: Tth111 I. A B L M H Isoschizomers: Tth111 I. A B L M H
Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
326 326
To place an order: 0800 262 1640 or Technical inquiries: 800 262 4911 To place an order: 0800 262 1640 or Technical inquiries: 800 262 4911
www.roche-applied-science.com e-mail: biochems.us@roche.com www.roche-applied-science.com e-mail: biochems.us@roche.com
03
Anhang (Grau II) arabisch nummeriert
P Kreuztabelle Doppelseite
01 02 03 04 06 07
Trade- Sonder- Patent & Product Product Terms &
seiten Licence Index A-Z Index 0-9 Conditions
marks
Disclaimer
04
Publishing System generate InDesign pages
Indesign Book
3/15
KW So. 26/08/12](https://image.slidesharecdn.com/redaktionssysteme-2011-oyendekopie-120826091153-phpapp01/85/Redaktionssysteme-2011-15-320.jpg)
Uploaded byBüro joerg.oyen.de
Redaktionssysteme 2011
The document discusses redaktionssystems (content management systems) in German. It provides an overview of the history and focus areas of the company oyen.de, which was founded in 2001 and specializes in consulting, training, and programming solutions for publishing challenges. It then discusses key aspects of content management systems like the path from data to layout and output, different system approaches and overlaps, flexible versus automated layouts, and components of collaboration.
More Related Content
Redaktionssysteme 2011
- 1. »Redaktionssysteme« oyen.de | 2011 KW So. 26/08/12
- 2. im Detail Mögliche KWSo. 26/08/12 Wege von Daten, über Layout zu unterschiedlichen
- 3. Eckdaten Gründung oyen.de 2001 Schwerpunkt als Berater, Trainer & Medienprogrammierer Lösungsfindung für Publishing Herausforderungen Schwerpunkte als Autor Berichterstattung über Trends, Software und Technologien die das systemgestützte Publizieren in die Ausgabekanäle Print, Web und mobile Endgeräte beeinflussen [Multi Channel Publishing] 0/3 KW So. 26/08/12
- 4. Zeitstrahl DBP webasierte InBetween / Einführung Werbemit- Einrichtung / ePaper & DBP telprodu- Training & mobile 1. InDesign ktion via Markteinführ Devices Konferenz W2P ung 2010 2009 2007 2005 2004 2001 1999 1994 1986 Print2iPad / Katalog- 1. DBP CD-ROM DTP mit digital trends Print Marktüber- Kataloge Aldus Publishing goes Web / sicht Adobe auf PDF- Pagemaker Multi InDesign / Basis / Channel QuarkX- BluePrint Publishing / Press Search&Or Collabo- der 0/4 ration KW So. 26/08/12
- 5. CRM 360° Publishing Blick PIM PRINT Publishing WEB Systeme Datenbereit- MRM Ausgabe Gestalten & stellung Veredeln Kommunikation Werbemittel MAM DEVICES Verpackung Katalog / Anzeige Broschüren Katalog / Anzeige Broschüren Preisliste Preisliste Produkt Marken Handbuch Research Handbuch POS MaFo Handbuch POS POS E-Mailing E-Mailing Newsletter Newsletter Webshop Medien Information Manager Vehikel PRINT WEB Magalog Magalog Produkt finder DEVICES Social eBook eBook Spiel- Spiel Kiosk* Kiosk* Media konsolen consolen iPad mobile mobile mobile smart TV smart Tablet phone Radio phone E-Mailing E-Mailing Kommunikation Kunden- Kunden magazine Verlag Bücher magazine Bücher Newsletter Newsletter Geschäfts- Presse- Zeitung Magazin Geschäfts Pressemitteil Zeitung Magazin berichte mitteilungen berichte ungen 0/5 Information & Unterhaltung PR / Unternehmens - KW So. 26/08/12
- 6. Redaktionssysteme 1) Der Weg von Daten über Layout zur Ausgabe 2) Systemansätze und Überschneidungen 3) flexibles Layout ./. automatisiertes Layout 4) Komponenten 5) Zusammenarbeit 0/6 KW So. 26/08/12
- 7. 1 Der Weg von Daten über Layout zur Ausgabe KW So. 26/08/12
- 8. Zuweisungen Daten >Layout > Ausgabe Beispiele Ergebnis Seiten / Objekte Layout Daten / Asset Verknüpfung Programmierung Vorlagen Ausgabeformate Print Web Regelwerke Seitenaufbau / Umbuch mobile Katalog Endgeräte Magalog Seiten Objektaufbau / Verwendung Adaption Magazin Sprachen Stilvorgaben Absatz Formate Anzeige Kanal Zeichen Newsletter Grep Print Broschüre Print Konvertierung JPEG Preisliste Korrekturworkflow Abstimmung / Seitenformate Geschäftsberichte Freigabe PDF Print Statusüberwachung Web POS Bereitstellung PNG Remote Publishing / web2Print Office Verpackung Abgleichen Dokumentation Zusammenstellung Import CSV / XML Übernahme Dokumenten für Daten Ausgabe http:// Request Sammeln WebShop ODBC Web Portal Web Daten Filtern Layout Ausgabe (Kanal) bidirektional Aktualisierung Export RTF Konfigurator Inhalte Inhalte XML Newsletter Aufbereiten Seitenaufbau (Print) SlideShow Aufbereitung mit Daten Umsetzung Audio / Video Player iPad Vervollständigen Verknüpfen mit Daten Multimediales Interaktion und Assets aus Dateisystem - Animation Anreichern Tablet - Anwendung mobile mobile - E-Mail / URL - Schaltflächen / Navigation Endgeräte Endgeräte Seitenverweise Erstellung - Formulare Smartphone TOC / Index / Sprungziele Web/URL Web-Inhalte Querverweise eBook Popup-Windows Neuer Knoten scrollbare Bereiche KIOSK Reflow-Inhalte (ePub /iPad) oyen.de | 2011 1/8 KW So. 26/08/12
- 9. Zuweisungen Daten >Layout > Ausgabe Beispiele Ergebnis Seiten / Objekte Layout Daten / Asset Verknüpfung Programmierung Vorlagen Ausgabeformate Print Programmierung Web Regelwerke Vorlagen Seitenaufbau / Umbuch Katalog mobile Endgeräte Magalog Seiten Objektaufbau / Verwendung Adaption Katalog Magazin Sprachen Stilvorgaben Absatz Formate Magalog Anzeige Adaption Kanal Zeichen Magazin Formate Newsletter Grep Anzeige Print Broschüre Print Newsletter Konvertierung JPEG Print Broschüre Preisliste Print Korrekturworkflow Abstimmung / Seitenformate Geschäftsberichte Preisliste Konvertierung PDF Print Abstimmung / Freigabe Statusüberwachung Seitenformate Geschäftsberichte Freigabe Web POS Bereitstellung PNG Remote Publishing / web2Print POS Bereitstellung Office Verpackung Verpackung Abgleichen Dokumentation Zusammenstellung Dokumentation Abgleichen Import CSV / XML Übernahme Zusammenstellung Übernahme Dokumenten für Dokumenten für Daten Request Daten Ausgabe Ausgabe http:// Sammeln Sammeln WebShopWebShop ODBC Web Web Portal Portal WebWeb Daten Daten Filtern Filtern Layout Layout Ausgabe (Kanal) Ausgabe (Kanal) Aktualisierung Aktualisierung Export Export RTF Konfigurator bidirektional Konfigurator Inhalte Inhalte Inhalte Inhalte XML Newsletter Aufbereiten Newsletter Aufbereiten Seitenaufbau (Print) Umsetzung SlideShow iPad Vervollständigen Aufbereitung mit Daten Umsetzung Multimediales Audio / Video Player iPad Vervollständigen Verknüpfen mit Daten Anreichern Multimediales Interaktion Tablet und Assets aus Dateisystem - Animation mobile mobile Anreichern Endgerätemobile Tablet Endgeräte Erstellung - Anwendung Smartphone mobile - E-Mail / URL TOC / Index / - Schaltflächen / Navigation Endgeräte Endgeräte Seitenverweise Querverweise Erstellung - Formulare Smartphone eBook TOC / Index / Sprungziele Web/URL Web-Inhalte Querverweise KIOSK eBook Popup-Windows Neuer Knoten scrollbare Bereiche KIOSK Reflow-Inhalte (ePub /iPad) oyen.de | 2011 1/9 KW So. 26/08/12
- 10. 2 Systemansätze und Überschneidungen KW So. 26/08/12
- 11. Systemansätze und Überschneidungen ... Web Content Management System komplexe Verwaltung von Inhalten auf Internetseiten, kann in Richtung Printausgabe erweitert werden Printproduktionssystem effektive Erstellung von Printprodukten mittels Erweiterungen in Desktop Publishing Programmen, lösen sich von Print- orientierung Media Asset Managementsystem (Universelle) Verwaltung von Bildern, Grafiken und Produktionsdaten. Bieten Workflow- und Publishingfunktionalitäten bis hin zur Printausgabe 2/11 KW So. 26/08/12
- 12. 3 flexibles Layout ./. automatisiertes Layout KW So. 26/08/12
- 13. flexibles Layout ./.automatisiertes Layout Text um Bild schreiben Medienneutrale und redundanzfreie Speicherung der Inhalte erfolgt im Nachgang Ausgabe auf Knopfdruck Fokus liegt auf Verwaltung, Finden von Informationen, sichern von Fakten und Mehrfachverwendung von (Teil)inhalten. 3/13 KW So. 26/08/12
- 14. 01 Annotierung 1. Anmerkungen auf Layoutvorschau (Bild JPEG/PDF) von a) Layout b) Text c) Bild -> unabhängig von InDesign erstellen -> einblenden und abarbeiten erfolgt in InDesign 2. Speichern in WIS 3. Freigaben WIS 2.x System Push 02 Texte - Seitenplanung a) Übernahme a) Artikeltextvarianten b) Neugestaltung b) Verwendung c) neue Angebote c) Übergabe Manuskript Urtext / Änderungen - Briefings Rücklesen a) Layout b) Text c) Bild d) Angebote 03 Platzhalter e) Zusammen- stellungen für D-A-CH a) Preise - Abstimmungen b) Landesspezifische - Korrekturen Layoutelemente - Verwendung -> Befüllung per Workflow - Bereitstellung -> Übergabe Korrekte Best-Nr. Preis, Maße, etc. 04 Bilder Pull InDesign Doppelseite a) Top / Top-five (Vignetten) / Serienbilder c) Logos d) Stimmungsbilder -> Bilder für Litho sammeln -> Palette Briefing Bilder 05 Layout Status autom. Mitteilung / Statusaktion aus InDesign - Dokumentensperre - Teilsperren für Preise etc. - Verzeichnisüberwachung 3/14 KW So. 26/08/12
- 15. Cover / Umschlag Cover Vorwort Faxanfor derung Rücken 01 03 Rückseite Rückseite CMS Smile Publishing System 02 04 Publications Heiler Print Vorlaufseiten (Grau I) römisch nummeriert P ein Produkt pro Seite 6 LightCycler 480 System Instrument 6 LightCycler 480 System Instrument Manager Manager Product 4711 Cat. No. Pack Size Price Product 4711 Cat. No. Pack Size Price 03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50 (96 reactions) (96 reactions) Application Application 03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50 The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 into vectors allows rapid protein expression from wild-type or modified (96 reactions) into vectors allows rapid protein expression from wild-type or modified (96 reactions) DNA sequences. This method of generating DNA templates for protein ex- DNA sequences. This method of generating DNA templates for protein ex- pression avoids the time-consuming step of cloning into expression plasmids Contents pression avoids the time-consuming step of cloning into expression plasmids Contents and can be used for initial, rapid screening for expression. 1. Forward Primer and can be used for initial, rapid screening for expression. 1. Forward Primer 01 02 03 04 2. Reverse Primer 2. Reverse Primer Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Flexibly introduce desired mutations, change codon usage and generate ■ Flexibly introduce desired mutations, change codon usage and generate truncated proteins. truncated proteins. Product Description Product Description In order to generate linear expression constructs, two PCRs must be per- In order to generate linear expression constructs, two PCRs must be per- formed. In the first reaction, gene-specific primers are used to add overlap formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro- mixed with two flanking primers and DNA fragments coding for the T7 pro- motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod- (C-terminal or N-terminal). In the second overlap extension PCR, the prod- uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the ends are extended. This linear expression construct is then amplified via the flanking primers. Figure 1: Principle of the generation of PCR products for in flanking primers. Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. vitro expression using RTS Linear Template Generation Sets. product TOC all List of Real-Time PCR Systems Real-Time PCR Systems The introduction of an N-terminal His6-tag is shown. For detailed The introduction of an N-terminal His6-tag is shown. For detailed Background Information Background Information information on the mechanism, refer to the RTS website (www. information on the mechanism, refer to the RTS website (www. One of the major advantages of cell-free compared to cell-based expression One of the major advantages of cell-free compared to cell-based expression proteinexpression.com → RTS Wheat Germ Products → Linear proteinexpression.com → RTS Wheat Germ Products → Linear systems is the ability to use PCR-generated templates. Using the RTS Wheat systems is the ability to use PCR-generated templates. Using the RTS Wheat new Templates and Vectors for both N- and C-terminal tagging). Templates and Vectors for both N- and C-terminal tagging). Germ Linear Template Generation Set, His6-tag, if primers are designed for Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products duce a completely functional linear expression template for expression in Product 5088, see page 123 duce a completely functional linear expression template for expression in Product 5088, see page 123 either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided Linear Template Generation Set). Primers for the second PCR are provided literature chapters Intern. in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- sign of these kits enables easy transfer of products from any expression sys- sign of these kits enables easy transfer of products from any expression sys- Real-Time PCR Real-Time PCR tem to both the RTS Wheat Germ and E. coli platforms, as well as between tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to products into an RTS pIVEX Wheat Germ vector may be required (e.g., to products produce a template for expression scale-up to RTS 500 Wheat Germ CECF, produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments). used in a series of subsequent experiments). & support Represent 103 Ordering and product information, or technical support: www.pharma-enhance-science.com Subscriptions for electronic Pharmaica, product spotlights and more: www.pharma-enhance-science.com/enews Information on products available in bulk: scbio.pharma.com Bestellungen Gebührenfrei: 0800/235-3152 Fax: 0621/235-3136 Online Shop: www.IBuyPharma.de 103 Ordering and product information, or technical support: www.pharma-enhance-science.com Subscriptions for electronic Pharmaica, product spotlights and more: www.pharma-enhance-science.com/enews Information on products available in bulk: scbio.pharma.com Bestellungen Gebührenfrei: 0800/235-3152 Fax: 0621/235-3136 Online Shop: www.IBuyPharma.de material 01 P Produkt über 2 Seiten LightCycler 480 System LightCycler 480 System 6 Instrument 6 Instrument Product 4711 Cat. No. Pack Size Price Product 4711 Cat. No. Pack Size Price 03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50 (96 reactions) (96 reactions) Application Application 03 728 790 001 1 set 214,50 03 728 790 001 1 set 214,50 The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) The RTS Wheat Germ Linear Template Generation Set, His6-tag can be used (96 reactions) to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 to rapidly generate linear expression constructs by PCR, ready for use as 03 728 790 001 1 set 214,50 DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ (96 reactions) CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 CECF protein expression reactions. The use of PCR instead of subcloning 03 728 790 001 1 set 214,50 into vectors allows rapid protein expression from wild-type or modified (96 reactions) into vectors allows rapid protein expression from wild-type or modified (96 reactions) DNA sequences. This method of generating DNA templates for protein ex- DNA sequences. This method of generating DNA templates for protein ex- pression avoids the time-consuming step of cloning into expression plasmids Contents pression avoids the time-consuming step of cloning into expression plasmids Contents and can be used for initial, rapid screening for expression. 1. Forward Primer and can be used for initial, rapid screening for expression. 1. Forward Primer 2. Reverse Primer 2. Reverse Primer Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all Benefits 3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Rapidly create linear expression constructs. regulatory elements necessary for in vitro protein expression ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Optimize regulatory elements. 4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Save time; no need for laborious cloning. all regulatory elements necessary for in vitro protein expression ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Take advantage of flexible positioning of His6-tag. 5. Water, PCR-grade ■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Benefit by adding epitope-tag sequences for detection with antibodies. ■ Flexibly introduce desired mutations, change codon usage and generate ■ Flexibly introduce desired mutations, change codon usage and generate truncated proteins. truncated proteins. Product Description Product Description In order to generate linear expression constructs, two PCRs must be per- In order to generate linear expression constructs, two PCRs must be per- formed. In the first reaction, gene-specific primers are used to add overlap formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro- mixed with two flanking primers and DNA fragments coding for the T7 pro- Chapter 1-6 motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod- (C-terminal or N-terminal). In the second overlap extension PCR, the prod- uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the ends are extended. This linear expression construct is then amplified via the flanking primers. Figure 1: Principle of the generation of PCR products for in flanking primers. Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. vitro expression using RTS Linear Template Generation Sets. Real-Time PCR Systems Real-Time PCR Systems The introduction of an N-terminal His6-tag is shown. For detailed The introduction of an N-terminal His6-tag is shown. For detailed Background Information Background Information information on the mechanism, refer to the RTS website (www. information on the mechanism, refer to the RTS website (www. One of the major advantages of cell-free compared to cell-based expression One of the major advantages of cell-free compared to cell-based expression proteinexpression.com → RTS Wheat Germ Products → Linear proteinexpression.com → RTS Wheat Germ Products → Linear systems is the ability to use PCR-generated templates. Using the RTS Wheat systems is the ability to use PCR-generated templates. Using the RTS Wheat Templates and Vectors for both N- and C-terminal tagging). Templates and Vectors for both N- and C-terminal tagging). Germ Linear Template Generation Set, His6-tag, if primers are designed for Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products in the pack insert, the resulting product can be used in a second PCR to pro- Additional Products duce a completely functional linear expression template for expression in Product 5088, see page 123 duce a completely functional linear expression template for expression in Product 5088, see page 123 either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera- tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de- sign of these kits enables easy transfer of products from any expression sys- sign of these kits enables easy transfer of products from any expression sys- Real-Time PCR Real-Time PCR 01 02 03 04 tem to both the RTS Wheat Germ and E. coli platforms, as well as between tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments). used in a series of subsequent experiments). Chapter 103 Ordering and product information, or technical support: www.pharma-enhance-science.com Subscriptions for electronic Pharmaica, product spotlights and more: www.pharma-enhance-science.com/enews Information on products available in bulk: scbio.pharma.com Bestellungen Gebührenfrei: 0800/235-3152 Fax: 0621/235-3136 Online Shop: www.IBuyPharma.de 103 Ordering and product information, or technical support: www.pharma-enhance-science.com Subscriptions for electronic Pharmaica, product spotlights and more: www.pharma-enhance-science.com/enews Information on products available in bulk: scbio.pharma.com Bestellungen Gebührenfrei: 0800/235-3152 Fax: 0621/235-3136 Online Shop: www.IBuyPharma.de Num & Section Product Sonder Section Intro pages Seiten 02 index (TOC (4 Vari.) small) P mehre Produkte pro Seite Restriction Enzymes Restriction Enzymes 4 4 Asp EI Asp EI from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price 11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50 (10 U/µl) (10 U/µl) Compatible Ends: None. Compatible Ends: None. Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer with GC-RICH Solution: 100%. #not available in U.S. A B L M H with GC-RICH Solution: 100%. #not available in U.S. A B L M H Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Isoschizomers: Tth111 I. Isoschizomers: Tth111 I. Heat inactivation: No information available. Heat inactivation: No information available. Asp EI Asp EI from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price 11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50 (10 U/µl) (10 U/µl) Compatible Ends: None. Compatible Ends: None. Mapping and Cloning of Nucleic Acids Mapping and Cloning of Nucleic Acids Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Temperature Optimum: +37°C Buffer Temperature Optimum: +37°C Buffer Isoschizomers: Tth111 I. A B L M H Isoschizomers: Tth111 I. A B L M H Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Asp EI Asp EI from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price 11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50 (10 U/µl) (10 U/µl) Compatible Ends: None. Compatible Ends: None. Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Molecular Biology Molecular Biology Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Activity in PCR buffer: 0%. Restriction Site: GACN↓NNGTC Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplemented Buffer with GC-RICH Solution: 100%. #not available in U.S. A B L M H with GC-RICH Solution: 100%. #not available in U.S. A B L M H Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Temperature Optimum: +37°C 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Isoschizomers: Tth111 I. Isoschizomers: Tth111 I. Heat inactivation: No information available. Heat inactivation: No information available. Asp EI Asp EI from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price from Methanococcus aeolicus PL-15/H Cat. No. Pack Size Price 11 131 354 001 400 U 64,50 11 131 354 001 400 U 64,50 (10 U/µl) (10 U/µl) Compatible Ends: None. Compatible Ends: None. Isoschizomers: Tth111 I. Contents Isoschizomers: Tth111 I. Contents Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Methylation Sensitivity: No information available. Enzyme solution supplied with SuRE/Cut Buffer B Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Activity in PCR buffer: 100% Restriction Site: GACN↓NNGTC Temperature Optimum: +37°C Buffer Temperature Optimum: +37°C Buffer Isoschizomers: Tth111 I. A B L M H Isoschizomers: Tth111 I. A B L M H Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% Heat inactivation: No information available. 50 to 75% 100% 25 to 50% 75 to 100% 75 to 100% 326 326 To place an order: 0800 262 1640 or Technical inquiries: 800 262 4911 To place an order: 0800 262 1640 or Technical inquiries: 800 262 4911 www.roche-applied-science.com e-mail: biochems.us@roche.com www.roche-applied-science.com e-mail: biochems.us@roche.com 03 Anhang (Grau II) arabisch nummeriert P Kreuztabelle Doppelseite 01 02 03 04 06 07 Trade- Sonder- Patent & Product Product Terms & seiten Licence Index A-Z Index 0-9 Conditions marks Disclaimer 04 Publishing System generate InDesign pages Indesign Book 3/15 KW So. 26/08/12
- 16.
- 17. 4 Komponenten KW So. 26/08/12
- 18. Komponenten . Editorial System Erstellung und Bearbeitung von Content - Verwaltungswerkzeuge für Zugriffsunterstützung, Aufgabenverteilung und Statusüberwachung - Verarbeitungswerkzeuge für Contenterzeugung, properitär und/oder integration externen (Text)Editoren -> Layout-orientiert -> Zeilenfallverbindlich -> Content-orientiert -> strukturelle inhaltliche Aufbereitung 4/18 KW So. 26/08/12
- 19. Komponenten .. Content Repository Zentrale Speicherung der erstellten Inhalte - Trennung von Inhalt und Form -> Multi Format Publishing - Abbildung Makrostrukturen -> Inhaltebausteine referenzieren - Abbildung Mikrostrukturen -> Textkörper zerlegen - gleichzeitiger Zugriff verschiedener Benutzer auf Inhalte -> Check-Out -> Ein Benutzer bearbeitet Inhalt, Inhalt ist für andere Benutzer gesperrt -> Check-In -> neue Version wird geladen und zur Bearbeitung durch andere Benutzer freigeben 4/19 KW So. 26/08/12
- 20. Komponenten ... Publishing System Ausgabeseite des Content Management Systems - Printprodukte -> Seitenvorbereitung -> Ausschiessen -> Farbseparierung - Onlineprodukte -> Granularität der Gestaltung -> Template / StyleSheet / Skript -> Zeitpunkt der Erzeugung -> statisch oder dynamisch 4/20 KW So. 26/08/12
- 21. 5 Zusammenarbeit KW So. 26/08/12
- 22. mit jCatalog PIM& Cross Media Publisher Preislisten / Katalog 1. Planung & Steuerung Bilder Texte 2. Kreation Daten Publikations- Vorlagen- Layout- planung erstellung abstimmung CMS PIM 3. Repro Cross Media Publications Manager Publisher Bilderstellung Bildbearbeitung 4. Bilddatenmanagement Datenhandling Bilder / Texte CSV Daten InDesign.- PDF Dokumente 5. Reinzeichnung Seitenaufbau Text / Bild Aktualisierung Integration Abstimmung Lektorat Finishing Dokumenten- PDF Inh.verz. / TOC sammlung Druckdaten oyen.de | 2011 5/22 KW So. 26/08/12
- 23. Zusammenarbeit Termin- und Ablaufplanung Basis für strukturierte Prozesse und Abläufe -> Eskalationsstufen, Benachrichtigung und Aufgabendelegierung Echtzeitkommunikation Instant Messaging und Chat Whiteboarding Skizzieren von Anordnungen, Seitenaufbauten Annotierungen Ergänzend zu Annotierungen im PDF, webasierte, kollaborative Anmerkungen 5/23 KW So. 26/08/12
- 24.