2. Brief History of Recombinant DNA
1970s- it is when, the used of
recombinant DNA technology to
produce genetically engineered
organisms started with the
pioneering transfer of genes
between bacteria of same
Escherichia coli (E. coli) species.
3. 1982
- It is the year by which, the full
approval from national drug
regulatory authorities, notably the
US Food and Drug Administration
had received the protocol of
producing the first genetically
modified organism (Insulin).
4. What is Recombinant DNA?
• It is often shortened as rDNA.
• Molecules of DNA from the
different species that are inserted
into host organism to produce new
genetic combinations that are of
value to science, medicine,
agriculture and industry.
5. • It is an artificially made DNA
strand that is formed by the
combination of two or more gene
sequences.
• This new combination may or may
not occur naturally but is
engineered specifically for a
purpose to be used in one of the
many applications of rDNA.
6. Important Application of rDNA
1. Agricultural Application ( Crop
improvement)
a) Distant hybridization
b) Development of transgenic plants
c) development of root nodules in
cereal crops.
d) Development of C4 plants
7. Important Application of rDNA
2. Medical applications
a) Production of antibiotics
b) Production of hormones
c) Production of vaccines
d) Production of interferon
e) Production of enzymes
8. Important Application of rDNA
f) Gene theraphy
g) Solution of disputed parentage
h) Diagnosis of disease
i) Production of transgenic animals
9. Important Application of rDNA
3. Industrial applications
- rDNA techniques help in the
production of chemical compounds
of commercial importance,
improvement of existing
fermentation processes and
production of proteins from wastes.
