25:40 has historically focused on serving neglected and overlooked communities in South Africa. It began working with orphanages in Cape Town that were caring for children affected by AIDS. It then shifted its focus to more rural, underdeveloped areas like Hamburg and Canzibe in the Eastern Cape as these regions received less support. 25:40 supported organizations until they could become more self-sufficient and build capacity. A key theme has been maintaining a child-centered approach and focusing on initiatives that directly help orphans and vulnerable children.