This document discusses protease production from Aspergillus niger and explores cost effective carbon and nitrogen sources. It begins by defining proteases and explaining that they account for 60% of the total enzyme market. It then discusses how A. niger is well-suited for protease production due to its diverse habitat and ability to produce various enzymes for survival. The document explores solid-state fermentation as a preferred production method and examines different carbon and nitrogen sources, finding that rice bran or wheat bran and soybean meal are most cost effective. In summary, it focuses on optimizing protease production from A. niger through consideration of fermentation methods and inexpensive nutrient sources.

1. Quest for Cost Effective Carbon and Nitrogen Sources for
Protease Production from A. niger
NITINJAY SINGH
ID-17BTBIOCE011
SUB -ENZYME TECHNOLOGY
PROJECT TITLE

2. CONTENT
• What are proteases ?
• Why proteases is important ?
• How Protease are produce ?
• Why Aspergillus Niger is important in protease production ?
• Cost effective carbon and nitrogen source

3. What are proteases ?
• Proteases occur naturally in all organisms.
• Hydrolyzed peptide bonds.
• 60 % of total enzyme market.

4. CLASSIFICATION OF PROTEASES
• On the basis of site of action on protein
Proteases are broadly classified as endo- or exo-enzymes
• Based on the pH
They are referred to as acidic, neutral, or alkaline proteases.
• Further categorized as serine proteases, aspartic proteases, threonine proteases cysteine
proteases

5. Source of Protease
• Sources
• Animal Proteases: pancreatic trypsin, chymotrypsin, pepsin and rennin.
• Plant Proteases: Papain, keratinases, and ficin
• Microbial proteases: Fungal or Bacterial

6. Why Aspergillus Niger for Protease production ?
The fungi possess diverse habitat, grow ubiquitously and produce various
proteins/enzymes their survival.
Fungal proteases offer a distinct advantage over bacterial enzymes in terms of ease of
downstream processing
Fungal enzymes are commonly used in industries due to various technical reasons,
including the feasibility of obtaining enzymes at high concentration in the
fermentation medium .

7. PRODUCTION OF PROTEASES
• Solid-state fermentation is a preferred method for the production
of acid proteases by the fungi.

8. COST EFFECTIVE CARBON AND NITROGEN SOURCE
• Effect of different carbon sources
Rice bran or wheat bran is used for economic basis

9. Effect of different nitrogen sources
Effect of different nitrogen sources such as urea,
nutrient broth, meat extract, peptone and beef extract
were studied on the production of protease by A. niger
On the economic basis soya bean meal was selected as
nitrogen source

10. Why proteases are important ?

11. THANK YOU