This study investigated the effects of post-activation potentiation (PAP) using maximum isometric contractions on peak power output in college football players. Twelve players performed vertical jump and back squat tests both with and without preceding PAP. PAP involved a six-second maximum voluntary isometric contraction in the back squat position. Mean vertical jump and back squat peak powers were 8-12% higher with PAP but differences were not statistically significant. While PAP may provide small improvements in power, the study was underpowered and larger samples are needed to determine if PAP using maximum isometric contractions can effectively enhance athletic performance.