nkums-journal-v5n2p411-en

‫ﺷﻤﺎﻟﯽ‬ ‫ﺧﺮاﺳﺎن‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ‫ﻣﺠﻠﻪ‬Journal of North Khorasan University of Medical Sciences
‫ﺗﺎﺑﺴﺘﺎن‬1392‫؛‬5)2:(411-418411-418Summer2013;5(2):
‫ﯾﺎﺑﯽ‬ ‫ﻣﮑﺎن‬‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺳﻨﺘﺰﮐﻨﻨﺪه‬ ‫آﻧﺰﯾﻢ‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬ ‫اﯾﺰوﻓﺮم‬)eNOS(‫ﺑﺎﻓﺖ‬ ‫در‬
‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﻪ‬ ‫ﻣﺒﺘﻼ‬ ‫ﻣﺮدان‬ ‫ﺑﯿﻀﻪ‬
‫ﻧﻮﯾﻦ‬ ‫ﻏﻔﺎري‬ ‫ﻣﻌﺮﻓﺖ‬1
،‫ﻋﺎﺑﺪ‬ ‫ﻓﺮﻫﻨﮓ‬2
‫ﮐﯿﺎﻧﯽ‬ ‫ﻓﺎﻃﻤﻪ‬ ،3
،‫ﻓﻮﻗﯽ‬ ‫ﺧﺪﯾﺠﻪ‬4*
1
‫ﻋﻠﻤﯽ‬ ‫ﻫﯿﺎت‬ ‫ﻋﻀﻮ‬‫ﺗﺤﻘﯿﻘ‬ ‫ﻣﺮﮐﺰ‬‫ﻃﺎﻟﻘﺎﻧﯽ‬ ‫ﺑﯿﻤﺎرﺳﺘﺎن‬ ‫ﻧﺎﺑﺎروري‬ ‫ﺑﺎروري‬ ‫ﺑﻬﺪاﺷﺖ‬ ‫ﺎت‬،‫ﺑﻬﺸﺘﯽ‬ ‫ﺷﻬﯿﺪ‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬،‫اﯾﺮان‬ ،‫ﺗﻬﺮان‬
2
‫ﺻﺒﻮﺣﯽ‬ ‫ﻧﺎﺑﺎروري‬ ‫ﻣﺮﮐﺰ‬،‫ﺗﻬﺮان‬ ‫ﻣﻬﺪﯾﻪ‬ ‫ﺑﯿﻤﺎرﺳﺘﺎن‬.‫ﻣﺮﮐﺰ‬‫ﻧﺎﺑﺎروي‬ ‫و‬ ‫ﺑﺎروري‬ ‫ﺑﻬﺪاﺷﺖ‬ ‫ﺗﺤﻘﯿﻘﺎت‬‫اﯾﺮان‬ ،‫ﺗﻬﺮان‬ ،
3
‫ﺑﯿﻮﺷﯿﻤﯽ‬ ‫ارﺷﺪ‬ ‫ﮐﺎرﺷﻨﺎس‬،‫ﺑﻬﺸﺘﯽ‬ ‫ﺷﻬﯿﺪ‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬‫اﯾﺮان‬ ،‫ﺗﻬﺮان‬ ،
4
‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ‫ﻋﻠﻤﯽ‬ ‫ﻫﯿﺎت‬ ‫ﻋﻀﻮ‬‫ﺗﺸﺮﯾﺤﯽ‬ ‫ﻋﻠﻮم‬ ‫دﭘﺎرﺗﻤﺎن‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫داﻧﺸﮑﺪه‬ ،‫ﺷﻤﺎﻟﯽ‬ ‫ﺧﺮاﺳﺎن‬‫اﯾﺮان‬ ،‫ﺗﻬﺮان‬ ،
‫ﻣﺴﺌﻮل‬ ‫ﻧﻮﯾﺴﻨﺪه‬:‫ﺗﺸﺮﯾﺤﯽ‬ ‫ﻋﻠﻮم‬ ‫دﭘﺎرﺗﻤﺎن‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫داﻧﺸﮑﺪه‬ ،‫ﺷﻤﺎﻟﯽ‬ ‫ﺧﺮاﺳﺎن‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ‫ﻋﻠﻤﯽ‬ ‫ﻫﯿﺎت‬ ‫ﻋﻀﻮ‬
‫اﻟﮑﺘﺮوﻧﯿﮏ‬ ‫ﭘﺴﺖ‬:alashtlake@gmail.com
‫وﺻﻮل‬:2/2/92‫اﺻﻼح‬:19/4/92‫ﭘﺬﯾﺮش‬:16/6/92
‫ﭼﮑﯿﺪه‬
‫زﻣﯿﻨﻪ‬‫وﻫﺪف‬:‫ﮐﻪ‬ ‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﻣﺮداﻧﻪ‬ ‫ﻋﻠﻞ‬ ‫ﺑﺎ‬ ‫ﻧﺎﺑﺎروري‬ ‫ﻫﺎي‬ ‫ﻋﻠﺖ‬ ‫از‬ ‫ﯾﮑﯽ‬‫اﯾﻦ‬‫ﺑﯿﻀﻮي‬ ‫ﭘﺲ‬ ‫اﺧﺘﻼﻻت‬ ‫ﺟﺰء‬ ‫اﺧﺘﻼل‬)post
testicular(‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬.‫ﻣﻄﺎ‬ ‫ﻃﺒﻖ‬‫ﭘﺬﯾﺮﻓﺖ‬ ‫ﺻﻮرت‬ ‫ﻗﺒﻼ‬ ‫ﮐﻪ‬ ‫ﻣﺸﺎﺑﻬﯽ‬ ‫ﻟﻌﺎت‬‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺎن‬ ‫از‬ ‫ﻣﺘﻔﺎوﺗﯽ‬ ‫ﻣﻘﺎدﯾﺮ‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﯿﻤﺎران‬eNOS‫ﺧﻮد‬ ‫از‬ ‫را‬
‫دﻫﻨﺪ‬ ‫ﻣﯽ‬ ‫ﻧﺸﺎن‬.‫ﻫﻤﯿﻦ‬ ‫ﺑﻪ‬‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺎن‬ ‫ﺑﺮرﺳﯽ‬ ‫ﺑﻪ‬ ‫ﮐﻪ‬ ‫ﺷﺪﯾﻢ‬ ‫آن‬ ‫ﺑﺮ‬ ‫دﻟﯿﻞ‬eNOS‫ﺑﭙﺮدازﯾﻢ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﯿﻤﺎران‬ ‫در‬.
‫و‬ ‫ﻣﻮاد‬‫روش‬‫ﮐ‬‫ﺎ‬‫ر‬:‫ﻣﻮردي‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬ ‫در‬–‫ﺷﺎﻫﺪي‬‫ﺗﻌﺪاد‬10‫و‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫درﮔﺮوه‬ ‫ﻧﻔﺮ‬7‫ﻧﻔﺮ‬‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫در‬)‫ﺑﺎرور‬ ‫ان‬‫د‬‫ﻣﺮ‬ ‫از‬(‫ﮐﻪ‬
‫و‬ ‫درﻣﺎن‬ ‫ﺟﻬﺖ‬‫ﻧﺎﺑ‬ ‫ﺗﺸﺨﯿﺺ‬ ‫ﯾﺎ‬‫ﻣﺮا‬ ‫ﻧﺎزاﯾﯽ‬ ‫ﻣﺮاﮐﺰ‬ ‫ﺑﻪ‬ ‫ﺎروري‬‫ﻧﻤﻮدﻧ‬ ‫ﺟﻌﻪ‬‫ﺪ‬‫ﺷﺪﻧﺪ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫وارد‬.‫ﺗﺤﺖ‬ ‫دوﮔﺮوه‬ ‫ﻫﺮ‬ ‫از‬ ‫ﮐﺘﺒﯽ‬ ‫ﻧﺎﻣﻪ‬ ‫رﺿﺎﯾﺖ‬ ‫ﮐﺴﺐ‬ ‫ﺿﻤﻦ‬
‫ﻗﺮارﮔﺮﻓﺘﻨﺪ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﯿﻮﭘﺴﯽ‬ ‫ﻋﻤﻞ‬.‫ﻧﻤﻮﻧﻪ‬ ‫ﺳﭙﺲ‬‫ﺑﻪ‬ ‫ﻫﺎ‬‫ر‬‫ارزﯾﺎﺑﯽ‬ ‫ﻣﻮرد‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫وش‬‫ﮔﺮﻓﺖ‬ ‫ﻗﺮار‬.
‫ﻫﺎ‬ ‫ﯾﺎﻓﺘﻪ‬:‫آﻧﺰﯾﻢ‬eNOS‫در‬‫و‬ ‫ﻻﯾﺪﯾﮓ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫در‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫ه‬‫ردو‬‫ﮔﺮوه‬‫ﺷﺪﻧﺪ‬ ‫ﺑﯿﺎن‬‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬ ‫وﻟﯽ‬‫ﻧﺸﺪ‬ ‫دﯾﺪه‬ ‫ﻧﺮﻣﺎل‬ ‫زاﯾﺎي‬.
‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺎن‬ ‫ﺑﺎرز‬ ‫ﺗﻔﺎوت‬ ‫ﺗﻨﻬﺎ‬eNOS‫اﺳﺖ‬ ‫ﺑﻮده‬ ‫ﮐﻨﺘﺮل‬ ‫از‬ ‫ﺑﯿﺸﺘﺮ‬ ‫اﻧﺴﺪادي‬ ‫ﮔﺮوه‬ ‫در‬ ‫ﺑﯿﺎن‬ ‫اﯾﻦ‬ ‫ﮐﻪ‬ ‫اﺳﺖ‬ ‫ﺑﻮده‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬.‫ﻧﺘﺎﯾﺞ‬ ‫اﯾﻦ‬
‫ﻣﻌﻨ‬ ‫آﻣﺎري‬ ‫ازﻟﺤﺎظ‬‫د‬ ‫ﯽ‬‫اﺳﺖ‬ ‫ارﺑﻮده‬)001/0(P<
‫ﮔﯿﺮي‬ ‫ﻧﺘﯿﺠﻪ‬:‫و‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫زا‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺑﺎ‬ ‫ﻫﺎ‬ ‫ﺳﻠﻮل‬ ‫اﯾﻦ‬ ‫ﻣﺘﻘﺎﺑﻞ‬ ‫ارﺗﺒﺎط‬‫در‬ ‫را‬ ‫ﻣﻬﻤﯽ‬ ‫ﺑﺎﻟﻘﻮه‬ ‫ﻧﻘﺶ‬ ‫اﺣﺘﻤﺎﻻ‬ ‫ﻧﯿﻔﺮ‬ ‫ﺳﻤﯽ‬ ‫ﺗﻮﺑﻮل‬ ‫ﯾﺎي‬
‫و‬ ‫اﺳﭙﺮم‬ ‫ﮐﯿﻔﯿﺖ‬‫دارد‬ ‫ﺑﺮﻋﻬﺪه‬ ‫ﻣﻮﻓﻖ‬ ‫ﺑﺎروري‬ ‫ﯾﮏ‬ ‫وﻗﻮع‬ ‫ﻧﻬﺎﯾﺘﺎ‬.
‫ﮐﻠﯿﺪي‬ ‫ﻫﺎي‬ ‫واژه‬:‫آ‬‫زواﺳﭙﺮﻣﯽ‬‫اﻧﺴﺪادي‬،‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬،eNOS،‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬
‫ﻣﻘﺪﻣﻪ‬
‫ﺣﺪود‬ ‫ﺟﻬﺎﻧﯽ‬ ‫آﻣﺎر‬ ‫ﻃﺒﻖ‬10‫اﻟﯽ‬15%‫داراي‬ ‫زوﺟﯿﻦ‬
‫ﺑﺎﺷﻨﺪ‬ ‫ﻣﯽ‬ ‫ﻧﺎﺑﺎروري‬ ‫ﻣﺸﮑﻼت‬]1.[‫ﻣﺮداﻧﻪ‬ ‫ﻋﻠﺖ‬ ‫ﺑﻪ‬ ‫ﻧﺎﺑﺎروري‬
‫اﺳﺖ‬ ‫داده‬ ‫اﺧﺘﺼﺎص‬ ‫ﺧﻮد‬ ‫ﺑﻪ‬ ‫را‬ ‫ﻧﺎﺑﺎروري‬ ‫ﻋﻠﻞ‬ ‫از‬ ‫ﻧﯿﻤﯽ‬ ‫ﺣﺪود‬
]2،1[.‫اﺳﺖ‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﻣﺮداﻧﻪ‬ ‫ﻧﺎﺑﺎروري‬ ‫ﻫﺎي‬ ‫ﻋﻠﺖ‬ ‫از‬ ‫ﯾﮑﯽ‬
]2[.‫ﮐﻪ‬ ‫ﮔﺮدد‬ ‫ﻣﯽ‬ ‫اﻃﻼق‬ ‫درﻣﺮدان‬ ‫وﺿﻌﯿﺘﯽ‬ ‫ﺑﻪ‬ ‫آزواﺳﭙﺮﻣﯽ‬
‫ﻧﺸﻮد‬ ‫ﯾﺎﻓﺖ‬ ‫اﺳﭙﺮﻣﯽ‬ ‫ﻫﯿﭻ‬ ‫ﺳﺎﻧﺘﺮﻓﯿﻮژﺷﺪه‬ ‫ﺳﻤﯿﻨﺎل‬ ‫ﻣﺎﯾﻊ‬ ‫در‬
]3[‫ﮐﻠﯽ‬ ‫ﮔﺮوه‬ ‫دو‬ ‫ﺑﻪ‬ ‫آزواﺳﭙﺮﻣﯽ‬‫ا‬‫وﻏﯿﺮاﻧﺴﺪادي‬ ‫ﻧﺴﺪادي‬
‫ﺷﻮد‬ ‫ﻣﯽ‬ ‫ﺗﻘﺴﯿﻢ‬]4[.‫ﻃﻮل‬ ‫در‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫در‬
‫اﻧﺴﺪاد‬ ‫اﻧﺰال‬ ‫ﺗﺎ‬ ‫ﺑﯿﻀﻪ‬ ‫از‬ ‫اﺳﭙﺮم‬ ‫ﺣﺮﮐﺖ‬ ‫ﻣﺴﯿﺮ‬‫ﺻﻮرت‬ ‫ﺑﻪ‬
‫ﻓﯿﺰﯾﮑﯽ‬‫در‬ ‫اﺳﺖ‬ ‫ﻃﺒﯿﻌﯽ‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬ ‫وﻟﯽ‬ ‫وﺟﻮددارد‬
‫ﯾﺎ‬ ‫ﮐﺎﻫﺶ‬ ‫ﺑﺎ‬ ‫ﺑﯿﻤﺎر‬ ‫ﻏﯿﺮاﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫در‬ ‫ﺣﺎﻟﯿﮑﻪ‬
‫ﻣﻮاﺟ‬ ‫ﺑﯿﻀﻪ‬ ‫در‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬ ‫ﻓﻘﺪان‬‫ﺷﺪﯾﺪ‬ ‫وﻧﻘﺺ‬ ‫ﺷﺪه‬ ‫ﻪ‬
‫وﺟﻮددارد‬ ‫ﺑﯿﻀﻮي‬.‫اﺛﺮ‬ ‫در‬ ‫ﺗﻮاﻧﺪ‬ ‫ﻣﯽ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬
‫دﯾﺪﯾﻤﯽ‬ ‫اﭘﯽ‬ ‫زاﯾﯽ‬ ‫ﺑﯿﻤﺎري‬،‫و‬ ‫وازال‬‫اﯾﺠﺎد‬ ‫اﻧﺰاﻟﯽ‬ ‫ﻣﺠﺎراي‬ ‫ﯾﺎ‬
‫ﮔﺮدد‬.‫واز‬ ‫اﻧﺴﺪاد‬ ‫ﻋﻠﻞ‬ ‫ﺗﺮﯾﻦ‬ ‫ﺷﺎﯾﻊ‬ ‫از‬ ‫ﯾﮑﯽ‬‫اﺳﺖ‬ ‫وازﮐﺘﻮﻣﯽ‬ ‫ال‬.
‫ﺑﻪ‬ ‫ﺗﻮان‬ ‫ﻣﯽ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﺮوز‬ ‫در‬ ‫ﺷﺎﯾﻊ‬ ‫ﻋﻠﻞ‬ ‫دﯾﮕﺮ‬ ‫از‬
‫ادرا‬ ‫ﺳﯿﺴﺘﻢ‬ ‫ﻫﺎي‬ ‫ﻋﻔﻮﻧﺖ‬‫ر‬‫ي‬-‫ﺗﻨﺎﺳﻠﯽ‬،‫ﻫﺎي‬ ‫ﺟﺮاﺣﺖ‬
‫ﻣﻘﺎﻟﻪ‬‫ﭘﮋوﻫﺸﯽ‬
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412‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺳﻨﺘﺰﮐﻨﻨﺪه‬ ‫آﻧﺰﯾﻢ‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬ ‫اﯾﺰوﻓﺮم‬ ‫ﯾﺎﺑﯽ‬ ‫ﻣﮑﺎن‬..‫و‬ ‫ﻧﻮﯾﻦ‬ ‫ﻏﻔﺎري‬ ‫ﻣﻌﺮﻓﺖ‬‫ﻫﻤﮑﺎران‬
‫ا‬‫واﯾﻨﮕﻮﺋﯿ‬ ‫ﺳﮑﺮوﺗﺎل‬‫و‬ ‫ﺟﺮاﺣﯽ‬ ‫ﻃﯽ‬ ‫در‬ ‫ﻨﺎل‬‫و‬ ‫ژﻧﺘﯿﮑﯽ‬ ‫ﻋﻮاﻣﻞ‬
‫ﻧﻤﻮد‬ ‫اﺷﺎره‬ ‫وارﯾﮑﻮﺳﻞ‬ ‫ﯾﺎ‬]5[.‫ﻧﻘﺶ‬ ‫داﻧﺸﻤﻨﺪان‬ ‫اﺧﯿﺮا‬
‫اﺣﺘﻤﺎﻟﯽ‬ ‫ﻋﺎﻣﻞ‬ ‫ﻋﻨﻮان‬ ‫ﺑﻪ‬ ‫ﻧﯿﺰ‬ ‫را‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬‫دﺧﯿﻞ‬‫در‬
‫اﻧﺪ‬ ‫داﻧﺴﺘﻪ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬]6[‫ﻣﻮﻟﮑﻮل‬ ‫اﯾﻦ‬‫ﺗﺒﺪﯾﻞ‬ ‫از‬
‫اﺳﯿﺪاﻣﯿﻨﻪ‬L-arginine‫و‬ ‫ﮐﻮﻓﺎﮐﺘﻮرﻫﺎ‬ ‫ﯾﮑﺴﺮي‬ ‫واﺳﻄﻪ‬ ‫ﺑﻪ‬
‫آﻧﺰﯾﻢ‬‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺳﻨﺘﺰﮐﻨﻨﺪه‬1
‫ﺑﻪ‬L-citruline‫و‬
‫ﮔﺮدد‬ ‫ﻣﯽ‬ ‫ﺗﺒﺪﯾﻞ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬]7[.‫ﺗﺎ‬‫ﺳﻪ‬ ‫ﺣﺎل‬ ‫ﺑﻪ‬
‫اﯾﺰوﻓﺮم‬NOS‫ﻧﻮروﻧﯽ‬ ‫ﻫﺎي‬ ‫ﻧﺎم‬ ‫ﺑﻪ‬-‫و‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬‫اﻟﻘﺎﺋﯽ‬
‫و‬ ‫ﺷﻨﺎﺳﺎﯾﯽ‬‫اﺳﺖ‬ ‫ﺷﺪه‬ ‫ﺗﺨﻠﯿﺺ‬]7[.‫ﻫﺎي‬ ‫اﯾﺰوﻓﺮم‬ ‫ﻣﯿﺎن‬ ‫در‬
NOS‫ا‬‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺳﻨﺘﺰﮐﻨﻨﺪه‬ ‫آﻧﺰﯾﻢ‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬ ‫ﯾﺰوﻓﺮم‬
‫ﮐﻠﺴﯿ‬ ‫ﺑﻪ‬ ‫واﺑﺴﺘﻪ‬‫اﺳﺖ‬ ‫ﺳﻠﻮﻟﯽ‬ ‫داﺧﻞ‬ ‫ﻢ‬]9،8[‫اﺧﯿﺮ‬ ‫ﻣﻄﺎﻟﻌﺎت‬
‫ﮐﻨﺪ‬ ‫ﻣﯽ‬ ‫ﭘﯿﺸﻨﻬﺎد‬‫ﻓﯿﺰﯾﻮﻟﻮژي‬ ‫دﺳﺘﮕﺎه‬ ‫در‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﮐﻪ‬
‫ﻋﻤﻠﮑﺮد‬ ‫ﻗﺒﯿﻞ‬ ‫از‬ ‫ﻣﺨﺘﻠﻔﯽ‬ ‫ﻓﯿﺰﯾﻮﻟﻮژﯾﮑﯽ‬ ‫ﻫﺎي‬ ‫ﻧﻘﺶ‬ ‫ﻣﺮداﻧﻪ‬
‫ﻧﻌﻮﻇﯽ‬،‫آﻧﺪروژن‬ ‫ﺗﺮﺷﺢ‬،‫اﺳﭙﺮم‬ ‫ﺣﺮﮐﺖ‬،‫اﺳﭙﺮم‬ ‫ﺑﻠﻮغ‬،‫ﮐﯿﻔﯿﺖ‬
‫اﺳﭙﺮم‬،‫اﺳﭙﺮم‬ ‫ﯾﺎﺑﯽ‬ ‫ﻇﺮﻓﯿﺖ‬،‫اﯾﻔﺎ‬ ‫را‬ ‫اﺳﭙﺮم‬ ‫ﺑﻪ‬ ‫ﺗﺨﻤﮏ‬ ‫اﺗﺼﺎل‬
‫ﮐﻨﺪ‬ ‫ﻣﯽ‬]10[.‫ﻣﮑ‬‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫در‬ ‫ﻣﻮﻟﮑﻮل‬ ‫اﯾﻦ‬ ‫ﯾﺎﺑﯽ‬ ‫ﺎن‬،‫اﭘﯽ‬
‫دﯾﺪﯾﻢ‬،‫در‬ ‫را‬ ‫ﻣﻮﻟﮑﻮل‬ ‫اﯾﻦ‬ ‫ﻧﻘﺶ‬ ‫وزﯾﮑﻮل‬ ‫وﺳﻤﯿﻨﺎل‬ ‫ﭘﺮوﺳﺘﺎت‬
‫و‬ ‫ﻋﺮوﻗﯽ‬ ‫ﺗﻌﺎدل‬ ‫ﺑﺮﻗﺮاري‬‫و‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬ ‫در‬‫اﺳﭙﺮم‬ ‫ﺑﻠﻮغ‬
‫ﮐﻨﺪ‬ ‫ﻣﯽ‬ ‫ﭘﯿﺸﻨﻬﺎد‬]10-8[‫ﺗﻮﻟﯿﺪﻣﺜﻠﯽ‬ ‫ﻫﺎي‬ ‫ﺳﯿﺴﺘﻢ‬‫ﺑﺎ‬
‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫ﻫﺎي‬ ‫ازروش‬ ‫اﺳﺘﻔﺎده‬‫آﻧﺰﯾﻢ‬eNOS
‫ﻣ‬‫ﮐﻨ‬ ‫ﻣﯽ‬ ‫ﭘﯿﺸﻨﻬﺎد‬ ‫اﺧﯿﺮ‬ ‫ﻄﺎﻟﻌﺎت‬‫آﻧﺰﯾﻢ‬ ‫اﯾﻦ‬ ‫ﺑﯿﺎن‬ ‫ﺪ‬‫ﻣﻬﻢ‬ ‫ﻧﻘﺶ‬
‫ﺑﺎﻓﺖ‬ ‫در‬ ‫را‬ ‫اي‬ ‫ﺑﺎﻟﻘﻮه‬‫دﯾﺪﯾﻢ‬ ‫اﭘﯽ‬ ‫ﻫﺎي‬،‫ﭘﺮوﺳﺘﺎت‬،‫ﮐﯿﺴﻪ‬
‫ﺳﻤﯿﻨﺎل‬ ‫ﻫﺎي‬،‫و‬‫ﺑ‬ ‫ﻫﻤﭽﻨﯿﻦ‬‫و‬ ‫ﻣﻮش‬ ‫ﺑﯿﻀﻪ‬ ‫ﺎﻓﺖ‬‫اﯾﻔﺎ‬ ‫اﻧﺴﺎن‬
‫ﮐﻨﺪ‬ ‫ﻣﯽ‬]11[.‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫در‬eNOS‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬
‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ازﻗﺒﯿﻞ‬ ‫اﻧﺴﺎن‬ ‫ﻧﺮﻣﺎل‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫ﻣﺨﺘﻠﻒ‬
‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫اﻧﺪوﺗﻠﯿﺎل‬،‫ﻻﯾﺪﯾﮓ‬ ‫ﺳﻠﻮل‬ ‫در‬،‫در‬‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
‫ﺳﺮﺗﻮﻟﯽ‬،‫ﺳﻤﯽ‬ ‫ﺗﻮﺑﻮل‬ ‫اﻃﺮاف‬ ‫ﻫﺎي‬ ‫ﻣﯿﻮﻓﯿﺒﺮوﺑﻼﺳﺖ‬
‫اﺳﺖ‬ ‫ﺷﺪه‬ ‫ﯾﺎﺑﯽ‬ ‫ﻧﯿﻔﺮﻣﮑﺎن‬]10،7[.‫ﺻﻮرﺗﯽ‬ ‫در‬‫اﯾﻦ‬ ‫ﺑﯿﺎن‬ ‫ﮐﻪ‬
‫در‬ ‫آﻧﺰﯾﻢ‬‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫ﻣﺨﺘﻠﻒ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫ﺑﻪ‬ ‫ﻣﺒﺘﻼ‬ ‫ﻣﺮدان‬
‫اﺳﺖ‬ ‫ﻧﺸﺪه‬ ‫ﺑﺮرﺳﯽ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬.‫ﻟﺬا‬‫در‬‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬
‫ﻣﻨﻈﻮرﺑﺮرﺳﯽ‬ ‫ﺑﻪ‬‫و‬ ‫ﺑﯿﺎن‬ ‫ﺑﺮرﺳﯽ‬‫ﻧﻘﺶ‬‫اﻧﺰﯾﻢ‬eNOS‫در‬
‫ﺑﯿﻤﺎر‬‫ا‬‫ن‬‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬‫آﻧﺰﯾﻢ‬ ‫ﯾﺎﺑﯽ‬ ‫ﻣﮑﺎن‬ ‫ﺑﺮرﺳﯽ‬ ‫ﺑﻪ‬
eNOS‫اﻓﺮاد‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫در‬‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬‫ﺑﺎ‬ ‫وﺑﯿﻤﺎران‬‫اﺧﺘﻼل‬
‫آزواﺳﭙﺮﻣﯽ‬‫اﻧﺴﺪادي‬‫ﻗﺮارﮔﺮﻓﺘﻨﺪ‬.
‫ش‬ ‫رو‬‫ﮐﺎر‬
1 -Nitric oxide synthase (NOS)
‫ﻣﻮردي‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬ ‫در‬–‫ﺗﻌﺪاد‬ ‫ﺷﺎﻫﺪي‬10‫ﻣﺮدان‬ ‫از‬ ‫ﻧﻔﺮ‬
‫و‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﻪ‬ ‫ﻣﺒﺘﻼ‬7‫ﺑﺎﻓﺖ‬ ‫ﺑﺎ‬ ‫ﻣﺮدان‬ ‫از‬ ‫ﻧﻔﺮ‬
‫ﻃﺒﯿﻌﯽ‬ ‫ﺑﯿﻀﻪ‬‫ﻣﻮرد‬‫ﺑﺮر‬‫ﮔﺮﻓﺘﻨﺪ‬ ‫ﻗﺮار‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫ﺳﯽ‬.
‫از‬‫دو‬ ‫اﯾﻦ‬‫ﮔﺮوه‬‫ﻣﺠﻮز‬ ‫ﮐﺴﺐ‬ ‫از‬ ‫ﭘﺲ‬‫از‬‫اﺧﻼق‬ ‫ﮐﻤﯿﺘﻪ‬
‫ﺷﻬﯿﺪ‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬‫و‬ ‫ﺑﻬﺸﺘﯽ‬‫ﮐﺘﺒﯽ‬ ‫ﻧﺎﻣﻪ‬ ‫رﺿﺎﯾﺖ‬‫از‬
‫ﺑﯿ‬‫روش‬ ‫ﺑﻪ‬ ‫ﺑﺮداري‬ ‫ﻧﻤﻮﻧﻪ‬ ‫ﻣﺒﺘﻼ‬ ‫ﻤﺎران‬‫ﺑﯿﻀﻪ‬ ‫ﺑﯿﻮﭘﺴﯽ‬2
‫ﺻﻮرت‬
‫ﭘﺬﯾﺮﻓﺖ‬.‫ﻗﺎﺑﻞ‬‫در‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﯿﻮﭘﺴﯽ‬ ‫ﻋﻤﻞ‬ ‫ﮐﻪ‬ ‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫ذﮐﺮ‬
‫ﺣﯿﻦ‬‫و‬ ‫درﻣﺎن‬‫ﯾﺎ‬‫ﺑﺮاي‬ ‫ﺗﺸﺨﯿﺺ‬‫اﻧﺠ‬‫ﮐﻤﮏ‬ ‫ﻫﺎي‬ ‫ﺗﮑﻨﯿﮏ‬ ‫ﺎم‬
‫اﺳﭙﺮم‬ ‫ﺑﻪ‬ ‫دﺳﺘﺮﺳﯽ‬ ‫ﺑﺮاي‬ ‫ﺑﺎروري‬‫را‬ ‫ﺧﻄﺮي‬ ‫و‬ ‫ﺷﺪه‬ ‫اﻧﺠﺎم‬
‫ﺑﯿﻤﺎر‬ ‫ﻣﺘﻮﺟﻪ‬‫ﻧﻨﻤﻮد‬.
‫ﻫﺎ‬ ‫ﺑﺎﻓﺖ‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬:
‫ﻗﺒﻠﯽ‬ ‫ﻣﻄﺎﻟﻌﺎت‬ ‫اﺳﺎس‬ ‫ﺑﺮ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬ ‫در‬ ‫ﮔﺮﻓﺘﻪ‬ ‫ﮐﺎر‬ ‫ﺑﻪ‬ ‫روش‬
‫ﭘﺬﯾﺮﻓﺖ‬ ‫اﻧﺠﺎم‬]9.[‫و‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫ﺑﯿﻮﭘﺴﯽ‬ ‫از‬ ‫ﭘﺲ‬‫ﺗﺸﺨﯿﺺ‬
‫آزواﺳﭙﺮﻣﯽ‬ ‫اﻧﺴﺪادي‬ ‫ﻧﻮع‬)‫ﺑﯿ‬ ‫ﻧﻤﻮﻧﻪ‬ ‫در‬ ‫اﺳﭙﺮم‬ ‫وﺟﻮد‬‫ﻮﭘﺴﯽ‬
‫ﺷﺪه‬‫و‬‫ﺳﻤﯿﻨﺎل‬ ‫ﻣﺎﯾﻊ‬ ‫ﻧﻤﻮﻧﻪ‬ ‫در‬ ‫اﺳﭙﺮم‬ ‫وﺟﻮد‬ ‫ﻋﺪم‬‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬
‫و‬ ‫ﺑﻮﺋﯿﻦ‬ ‫ﻓﯿﮑﺴﺎﺗﯿﻮ‬ ‫ﻣﺤﻠﻮل‬ ‫در‬‫از‬ ‫ﭘﺲ‬4‫ﻣﺤﻠﻮل‬ ‫در‬ ‫ﺳﺎﻋﺖ‬
‫ﻓﺮﻣﺎﻟﯿﻦ‬ ‫ﻓﯿﮑﺴﺎﺗﯿﻮ‬10%‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫ﺑﺮرﺳﯽ‬ ‫ﺑﺮاي‬
‫ﻗﺮارداد‬‫ه‬‫ﺷﺪ‬.
‫ﻣﺮاﺣﻞ‬‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫اﻧﺠﺎم‬:
1.‫ﺑﺮ‬‫ﻫﺎي‬ ‫ش‬5‫از‬ ‫ﻣﯿﮑﺮوﻧﯽ‬‫ﭘﺎراﻓﯿﻨﯽ‬ ‫ﻫﺎي‬ ‫ﺑﻠﻮك‬‫ﻫﺎ‬ ‫ﺑﺎﻓﺖ‬‫و‬
‫داد‬ ‫ﻗﺮار‬‫ﺑﺎ‬ ‫ﺷﺪه‬ ‫دار‬ ‫ﭘﻮﺷﺶ‬ ‫ﻫﺎي‬ ‫ﻻم‬ ‫روي‬ ‫ﺑﺮ‬ ‫ن‬Poly- L-
Lysin.
2.‫زداﯾﯽ‬ ‫ﭘﺎراﻓﯿﻦ‬.
3.‫ﺳﺎزي‬ ‫ﺧﻨﺜﯽ‬‫زاد‬ ‫درون‬ ‫ﭘﺮاﮐﺴﯿﺪازﻫﺎي‬3
‫از‬ ‫اﺳﺘﻔﺎده‬ ‫ﺑﺎ‬
‫ﭘﺮاﮐﺴﯿﺪﻫﯿﺪروژن‬3‫درﺻﺪ‬‫و‬‫ﻣﺘﺎﻧﻮل‬)Merck
Germany(‫ﻣﺪت‬ ‫ﺑﻪ‬15‫ﺗﺎرﯾﮑﯽ‬ ‫در‬ ‫دﻗﯿﻘﻪ‬.
3.‫ﮐﺮدن‬ ‫ﺑﻠﻮﮐﻪ‬‫ﻫﺎ‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬‫اﺧﺘﺼﺎﺻﯽ‬ ‫ﻏﯿﺮ‬ ‫ي‬‫اﺳﺘﻔﺎده‬ ‫ﺑﺎ‬
‫ﺑﺰي‬ ‫ﺳﺮم‬ ‫از‬4
)DAKO Denmark(.
4.‫ﮐﺮدن‬ ‫ﺑﻠﻮﮐﻪ‬‫زاد‬ ‫درون‬ ‫ﻫﺎي‬ ‫ﺑﯿﻮﺗﯿﻦ‬5
)Merck
Germany(‫ﺑﺎ‬ ‫ﻧﯿﺰ‬‫ﮐﻨﻨ‬ ‫ﺑﻠﻮك‬ ‫ﻣﺤﻠﻮل‬ ‫از‬ ‫اﺳﺘﻔﺎده‬‫ﺑﯿﻮﺗﯿﻦ‬ ‫ﺪه‬
‫ﻣﺪت‬ ‫ﺑﻪ‬15‫دﻗﯿﻘﻪ‬.
5.‫ﻫﺎ‬ ‫ﻧﻤﻮﻧﻪ‬ ‫ﺗﯿﻤﺎر‬‫اﻃﺮا‬‫اﺳﺘﻔﺎده‬ ‫ﺑﺎ‬ ‫ﻫﺎ‬ ‫ﻻم‬ ‫ف‬‫از‬‫اوﻟﯿﻪ‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬
‫آﻧﺰﯾﻢ‬ ‫اﻧﺴﺎﻧﯽ‬ ‫ﺿﺪ‬ ‫ﺧﺮﮔﻮﺷﯽ‬ ‫ﮐﻠﻮﻧﺎل‬ ‫ﭘﻠﯽ‬eNOS)Rabbit
2 -Testicular Biopsy
3 -Endogenous peroxidase
4- Goat serum
5 -Endogenous Biotin

‫ﺷﻤﺎﻟﯽ‬ ‫ﺧﺮاﺳﺎن‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ‫ﻣﺠﻠﻪ‬‫ﺗﺎﺑﺴﺘﺎن‬1392‫؛‬)2(5413
anti human eNOSAb cam cat num≠ ab5589
USA)(‫ﺑﺎرﻗﺖ‬50:1‫ﻣﺪت‬ ‫ﺑﻪ‬1‫اﻧﮑﻮﺑﺎﺗﻮر‬ ‫در‬ ‫ﺳﺎﻋﺖ‬.
6.‫ﺷﺴﺘﺸﻮ‬
7.‫ﺑﺎ‬ ‫ﺗﯿﻤﺎر‬‫ﺛﺎﻧﻮﯾﻪ‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬‫ﺑﯿﻮﺗﯿﻨﻪ‬ ‫ﺿﺪﺧﺮﮔﻮﺷﯽ‬(Goat
anti rabbit/bitinated))cat num≠ab 6721USA
Ab cam(‫ﺑﺎرﻗﺖ‬200:1‫ﺑ‬‫ﻣﺪت‬ ‫ﻪ‬1‫اﻧﮑﻮﺑﺎﺗﻮر‬ ‫در‬ ‫ﺳﺎﻋﺖ‬.
8.‫ﺷﺴﺘﺸﻮ‬
9.‫ﺑﺎ‬ ‫ﺗﯿﻤﺎر‬‫ﺛﺎﻟﺜﯿﻪ‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬(Straptividin /HRP(sigma
cat num ≠s 2341)‫رﻗﺖ‬ ‫ﺑﺎ‬2000:1‫ﻣﺪت‬ ‫ﺑﻪ‬30‫دﻗﯿﻘﻪ‬
10.‫ﺑﺎ‬ ‫ﻣﻮاﺟﻬﻪ‬‫دي‬ ‫رﻧﮕﯽ‬ ‫ﻣﺎده‬–‫ﺑﻨﺰﯾﺪﯾﻦ‬ ‫آﻣﯿﻨﻮ‬
1
(DAB)(DAKO Denmark).
11.‫ﻧﻤﻮﻧﻪ‬‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬ ‫ﺟﺎي‬ ‫ﺑﻪ‬ ‫ﮐﺎر‬ ‫ﺗﺄﺋﯿﺪ‬ ‫ﺟﻬﺖ‬ ‫ﻣﻨﻔﯽ‬ ‫ﮐﻨﺘﺮل‬
‫ﺑﺎ‬ ‫اوﻟﯿﻪ‬PBS‫ﺷﺴﺘﺸﻮ‬‫ﮔﺮدﯾﺪ‬.
12.‫ﻧﻤﻮﻧﻪ‬‫اﻧﺴﺎﻧﯽ‬ ‫ﺟﻔﺖ‬ ‫ﻫﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ ‫از‬ ‫ﺧﺎرﺟﯽ‬ ‫ﻣﺜﺒﺖ‬ ‫ﮐﻨﺘﺮل‬
‫ﻣﺎد‬‫راﻧﯽ‬‫ﮐﻪ‬‫زاﯾﻤﺎن‬Full –term‫داﺷﺘﻨﺪ‬‫ا‬‫ﮔﺮدﯾﺪ‬ ‫ﺳﺘﻔﺎده‬.
‫ﻫﺎي‬ ‫ﮐﺪ‬ ‫داراي‬ ‫ﮐﻪ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫ﻣﻮرد‬ ‫ي‬ ‫ﻫﺎ‬ ‫ﻧﻤﻮﻧﻪ‬ ‫ﭘﺎﯾﺎن‬ ‫در‬
‫ﺑﻮد‬ ‫ﻣﺨﻔﯽ‬‫ﻣﺤﻘﻖ‬ ‫ﺳﻪ‬ ‫ﺗﻮﺳﻂ‬ ‫ﻧﺪ‬‫و‬ ‫ﮔﺮﻓﺘﻪ‬ ‫ﻗﺮار‬ ‫ﺑﺮرﺳﯽ‬ ‫ﻣﻮرد‬
‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﺷﺪت‬ ‫ﺑﺮاﺳﺎس‬‫ﺷﺪﯾﺪ‬(+++)،‫ﻣﺘﻮﺳﻂ‬(++)،
‫ﺧﻔﯿﻒ‬(+)‫واﮐﻨﺶ‬ ‫ﺑﺪون‬ ‫و‬)0(‫ﮔﺮدﯾﺪ‬ ‫ﮔﺬاري‬ ‫ﻧﻤﺮه‬]9-
12[.
1 -3,3́ daiamno benzidine
‫آﻣﺎ‬ ‫روش‬‫ري‬:
‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷ‬ ‫از‬ ‫ﺣﺎﺻﻞ‬ ‫ﻧﺘﺎﯾﺞ‬ ‫آﻣﺎري‬ ‫ﺑﺮرﺳﯽ‬‫ﯿ‬‫دوﮔﺮوه‬ ‫ﻤﯽ‬
‫ﻏﯿﺮﭘﺎراﻣﺘﺮي‬ ‫آزﻣﻮن‬ ‫از‬ ‫اﺳﺘﻔﺎده‬ ‫ﺑﺎ‬Kruscal wallis‫ﺗﺠﺰﯾﻪ‬
‫ﮔﺮدﯾﺪ‬ ‫ﺗﺤﻠﯿﻞ‬.
‫ﻫﺎ‬ ‫ﯾﺎﻓﺘﻪ‬
‫ﻻﯾﺪﯾﮓ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬،‫ﺳﺮﺗﻮﻟﯽ‬‫ﺑﻪ‬ ‫ﮐﻪ‬ ‫ﻧﺎﺑﺎﻟﻎ‬ ‫اﺳﭙﺮﻣﺎﺗﯿﺪﻫﺎي‬
‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﺑﺎﺷﻨﺪ‬ ‫ﻣﯽ‬ ‫آﭘﻮﭘﺘﻮزﯾﺲ‬ ‫ﺣﺎل‬ ‫در‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﻧﻈﺮ‬
eNOS‫دادﻧﺪ‬ ‫ﻧﺸﺎن‬)‫ﺗﺼﺎوﯾﺮ‬2‫و‬1(.‫ﺳﻠﻮل‬ ‫ﮔﺮوه‬ ‫دو‬ ‫ﻫﺮ‬ ‫در‬
‫رﻧﮓ‬ ‫ﺷﺪت‬ ‫ﻣﯿﺎﻧﮕﯿﻦ‬ ‫ﻫﺎ‬ ‫ﺗﻮﺑﻮل‬ ‫اﮐﺜﺮ‬ ‫در‬ ‫ﻧﺮﻣﺎل‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬
‫ﺻﻔﺮ‬ ‫ﭘﺬﯾﺮي‬‫ﺑﻮد‬‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﻫﺎ‬ ‫ﺗﻮﺑﻮل‬ ‫ﻣﻮارد‬ ‫ﺑﺮﺧﯽ‬ ‫در‬ ‫اﻣﺎ‬
‫داﺷﺘﻨﺪ‬ ‫ﺿﻌﯿﻔﯽ‬.‫آزواﺳﭙﺮﻣﯽ‬ ‫ﮔﺮوه‬ ‫در‬ ‫ﻻﯾﺪﯾﮓ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
‫اﻧﺴﺪادي‬‫و‬‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬‫ﯾﮑﺴﺎﻧﯽ‬‫اﺳﺖ‬ ‫داﺷﺘﻪ‬
)‫ﺟﺪول‬1(‫ﭘﺬ‬ ‫رﻧﮓ‬ ‫ﻣﻼﺣﻈﻪ‬ ‫ﻗﺎﺑﻞ‬ ‫ﺗﻔﺎوت‬ ‫ﺗﻨﻬﺎ‬‫ﯾﺮي‬‫ﺳﻠﻮل‬
‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬‫در‬ ‫ﮐﻪ‬ ‫ﺑﻮده‬‫ﺷﺪت‬ ‫اﻧﺴﺪادي‬ ‫زواﺳﭙﺮﻣﯽ‬ ‫آ‬ ‫ﮔﺮوه‬
‫اﺳﺖ‬ ‫داﺷﺘﻪ‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫ﺑﻪ‬ ‫ﻧﺴﺒﺖ‬ ‫را‬ ‫ﺑﯿﺸﺘﺮي‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬
)‫ﺟﺪول‬2(.‫ﭘﯿﮑﻨﻮﺗﯿ‬ ‫ﻫﺴﺘﻪ‬ ‫ﺑﺎ‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫ﮏ‬
‫ﺳﻠﻮل‬ ‫ﺑﻪ‬ ‫ﺑﯿﺸﺘﺮ‬ ‫ﮐﻪ‬ ‫وﻫﺘﺮوﮐﺮﻣﺎﺗﯿﻦ‬‫ﺣﺎل‬ ‫در‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬
‫اﻧﺴﺪادي‬ ‫ﮔﺮوه‬ ‫در‬ ‫اﺳﺖ‬ ‫ﺷﺒﯿﻪ‬ ‫آﭘﻮﭘﺘﻮز‬‫و‬‫ﮐﻨﺘﺮل‬‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬
‫ﯾﮑﺴﺎﻧﯽ‬‫د‬‫ا‬ ‫اﺷﺘﻪ‬‫ﻧﺪ‬)‫ﺗﺼﻮﯾﺮ‬2(.‫ﺑﺮ‬ ‫ﻋﻼوه‬‫ﻧﻈﺮ‬ ‫در‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
‫ﻋﺮوق‬ ‫اﻧﺪوﺗﻠﯿﻮم‬ ‫ﻣﺎﻧﻨﺪ‬ ‫ﻫﺎ‬ ‫ﺳﻠﻮل‬ ‫دﯾﮕﺮ‬ ‫ﻓﻮق‬ ‫ﺷﺪه‬ ‫ﮔﺮﻓﺘﻪ‬
‫و‬ ‫ﺑﯿﻀﻮي‬ ‫ﺧﻮﻧﯽ‬‫ﻧﯿﺰ‬ ‫ﻣﯿﻮﺋﯿﺪ‬ ‫ﻋﻀﻼﻧﯽ‬ ‫ﺷﺒﻪ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫از‬ ‫درﺟﺎﺗﯽ‬eNOS‫راﻧﺸﺎن‬‫داد‬)‫ﺗﺼﻮﯾﺮ‬1‫و‬2(.
‫ﺟﺪول‬1
‫ﮔﺮوه‬/‫ﺳﻠﻮل‬‫ﻻﯾ‬ ‫ﺳﻠﻮل‬‫ﺪﯾﮓ‬‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﺳﻠﻮل‬
‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬+++
‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﮔﺮوه‬++++
‫آﻧﺰﯾﻢ‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬eNOS‫درﺳﻠﻮل‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﯿﻮﭘﺴﯽ‬ ‫از‬ ‫ﺣﺎﺻﻞ‬ ‫ﻫﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ ‫در‬‫دو‬ ‫ﻫﺎي‬‫و‬ ‫اﻧﺴﺪادي‬ ‫ﮔﺮوه‬‫ﮐﻨﺘﺮل‬:
)0:‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﻋﺪم‬:+‫ﺿﻌﯿﻒ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬:++‫ﻣﺘﻮﺳﻂ‬ ‫ﺷﺪت‬ ‫ﺑﺎ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬:+++‫ﺷﺪﯾﺪ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬(.‫ﺑﯿﺎن‬eNos‫در‬
‫ﮔﺮوه‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫داد‬ ‫ﻧﺸﺎن‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫ﺑﺎ‬ ‫داري‬ ‫ﻣﻌﻨﯽ‬ ‫اﺧﺘﻼف‬ ‫اﻧﺴﺪادي‬ ‫اﺳﭙﺮﻣﯽ‬ ‫آزو‬)001/0P<(

‫ﺟﺪول‬2
‫ﮔﺮوه‬/‫ﺳﻠﻮل‬‫ﺳﯿﺘﻮﭘﻼﺳﻢ‬
‫اﺳﭙﺮﻣﺎﺗﯿﺪﻧﺎﺑﺎﻟﻎ‬
‫ﻃﺒﯿﻌﯽ‬ ‫زاﯾﺎي‬ ‫ﺳﻠﻮل‬‫ﭘﯿﮑﻨﻮﺗﯿﮏ‬ ‫ﻫﺴﺘﻪ‬ ‫ﺑﺎ‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬)‫اﺑﻨﺮﻣﺎل‬(
‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬+0+
‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﮔﺮوه‬+0+
‫آﻧﺰﯾﻢ‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬eNOS‫ﺑﯿﻮﭘﺴﯽ‬ ‫از‬ ‫ﺣﺎﺻﻞ‬ ‫ﻫﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ ‫در‬‫ﺟﻨﺴﯽ‬ ‫ﻫﺎي‬ ‫درﺳﻠﻮل‬ ‫ﺑﯿﻀﻪ‬‫اﻧﺴﺪادي‬ ‫دوﮔﺮوه‬
‫وﮐﻨﺘﺮل‬:
)0:‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﻋﺪم‬:+‫ﺿﻌﯿﻒ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬:++‫ﻣﺘﻮﺳﻂ‬ ‫ﺷﺪت‬ ‫ﺑﺎ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬:+++‫ﺷﺪﯾﺪ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬.(‫در‬ ‫داري‬ ‫ﻣﻌﻨﯽ‬ ‫اﺧﺘﻼف‬
‫ﺑﯿﺎن‬eNOS‫ﻧﺪادﻧﺪ‬ ‫ﻧﺸﺎن‬)05/0p>(
‫ﺗﺼﻮﯾ‬‫ﺮ‬1:‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫آﻣﯿﺰي‬ ‫رﻧﮓ‬eNOS‫اﺳﭙﺮﻣﯽ‬ ‫آزو‬ ‫ﮔﺮوه‬ ‫در‬.‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
)‫ﭘﯿﮑﺎن‬(‫ﻟﯿﺪﯾﮏ‬ ،)،‫ﺳﻔﯿﺪ‬ ‫ﭘﯿﮑﺎن‬‫وﭼﭗ‬ ‫ﺑﺎﻻ‬(‫اﺳﭙﺮﻣﺎﺗﻮزوﺋﯿﺪ‬ ‫رده‬ ‫و‬)‫ﭘﯿﮑﺎن‬ ‫ﻧﻮك‬.(‫ﺑﺰرﮔﻨﻤﺎﯾﯽ‬40X

‫ﺑﺤﺚ‬
‫درﻣﻘﺎﯾﺴﻪ‬ ‫ﺣﺎﺿﺮ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫در‬‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺎن‬eNOS‫در‬‫اﻓﺮاد‬
‫و‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬‫زﯾﺎدي‬ ‫ﺗﻐﯿﯿﺮات‬ ‫ﺑﺎ‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬
‫و‬ ‫ﻧﺸﺪه‬ ‫ﻣﻮاﺟﻪ‬‫اﻓﺰاﯾﺶ‬ ‫ﮐﻪ‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬ ‫ﺟﺰ‬ ‫ﺑﻪ‬
‫اﯾﻦ‬ ‫ﻫﺎ‬ ‫ﺳﻠﻮل‬ ‫دﯾﮕﺮ‬ ‫در‬ ‫داﺷﺖ‬ ‫وﺟﻮد‬ ‫اﻧﺴﺪادي‬ ‫ﮔﺮوه‬ ‫در‬ ‫ﺑﯿﺎن‬
‫ﺑﯿﺎن‬‫ﮐﻤﯽ‬ ‫ﻧﯿﻤﻪ‬‫ﻣﺸﺎﺑﻪ‬ ‫ﺗﻘﺮﯾﺒﺎ‬‫ﺑﻮد‬.‫در‬ ‫اﯾﻦ‬‫ﮐﻪ‬ ‫اﺳﺖ‬ ‫ﺣﺎﻟﯽ‬
‫اي‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫در‬‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﯿﻤﺎران‬ ‫ﺑﺮروي‬ ‫ﻗﺒﻼ‬ ‫ﮐﻪ‬
‫داد‬ ‫اﻧﺠﺎم‬ ‫ﻏﯿﺮاﻧﺴﺪادي‬‫ا‬ ‫ه‬‫ﺳﻠﻮل‬ ‫ﮐﻞ‬ ‫در‬ ‫ﺑﯿﺎن‬ ‫ﺗﻐﯿﯿﺮات‬ ‫اﯾﻦ‬ ‫ﯾﻢ‬
‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫ﻫﺎي‬‫و‬ ‫ﺟﻨﺴﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫از‬ ‫اﻋﻢ‬‫ﻏﯿﺮﺟﻨﺴﯽ‬
‫ﻣﺘﻔﺎوت‬‫اﺳﺖ‬ ‫ﺑﻮده‬]15[.‫دﻟﯿﻞ‬ ‫اﯾﻦ‬ ‫ﺑﻪ‬ ‫اﺣﺘﻤﺎﻻ‬ ‫اﻣﺮ‬ ‫اﯾﻦ‬ ‫ﻋﻠﺖ‬
‫دﻟﯿﻞ‬ ‫ﺑﻪ‬ ‫اﻧﺴﺪادي‬ ‫ﻏﯿﺮ‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫اﻓﺮاد‬ ‫در‬ ‫ﮐﻪ‬ ‫اﺳﺖ‬‫ﻧﻤﺎﻫﺎي‬
‫ﺗﺎ‬ ‫ﻓﯿﺒﺮوز‬ ‫ﺗﻮﺑﻮل‬ ‫از‬ ‫ﻫﯿﺴﺘﻮﭘﺎﺗﻮﻟﻮژي‬ ‫ﻣﺨﺘﻠﻒ‬
‫در‬ ‫ﻧﻘﺺ‬ ‫ﻧﻤﺎﯾﺎﻧﮕﺮ‬ ‫ﮐﻪ‬ ‫ﻫﺎﯾﭙﻮاﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬
‫ﻓﺮاﯾﻨﺪاﺳﭙﺮﻣ‬‫ﭘﺮوﺗﺌﯿ‬ ‫ﺑﯿﺎن‬ ‫ﻣﯿﺰان‬ ‫ﻫﺴﺘﻨﺪ‬ ‫ﺎﺗﻮژﻧﺰﯾﺲ‬‫ﻦ‬eNOS
‫اﺳﭙﺮﻣﺎﺗﻮزﻧﺰﯾﺲ‬ ‫ﮐﻪ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﮔﺮوه‬ ‫ﺑﻪ‬ ‫ﻧﺴﺒﺖ‬
‫ﺑﯿﺸﺘﺮي‬ ‫ﺗﻐﯿﯿﺮات‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫ﺑﺎ‬ ‫ﻣﻘﺎﯾﺴﻪ‬ ‫در‬ ‫دارﻧﺪ‬ ‫ﻧﺮﻣﺎل‬
‫اﺳﺖ‬ ‫داﺷﺘﻪ‬.‫اﺳﭙ‬ ‫ﺑﺪ‬ ‫ﮐﯿﻔﯿﺖ‬‫ﺑﯿﻤﺎران‬ ‫در‬ ‫ﻫﺎ‬ ‫ﺮم‬‫آ‬‫زواﺳﭙﺮﻣﯽ‬
‫و‬ ‫اﻧﺴﺪادي‬‫ﻃﺮﯾﻖ‬ ‫از‬ ‫اﻧﺴﺪاد‬ ‫از‬ ‫ﭘﺲ‬ ‫ﺣﺎﻣﻠﮕﯽ‬ ‫ﮐﻢ‬ ‫ﻣﻮﻓﻘﯿﺖ‬
‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫ﻣﺸﻬﻮد‬ ‫وازﮐﺘﻮﻣﯽ‬،‫از‬ ‫ﻓﺎﮐﺘﻮرﻫﺎﺋﯽ‬ ‫ﺧﺎﻃﺮ‬ ‫ﻫﻤﯿﻦ‬ ‫ﺑﻪ‬
‫دﯾﺪﯾﻤﯽ‬ ‫اﭘﯽ‬ ‫اﺧﺘﻼﻻت‬ ‫ﻗﺒﯿﻞ‬،‫آ‬‫اﺳﭙﺮم‬ ‫ﺿﺪ‬ ‫ﻫﺎي‬ ‫ﺑﺎدي‬ ‫ﻧﺘﯽ‬1
‫و‬
‫ﻣﯽ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﻣﺎﻧﻨﺪ‬ ‫ﻫﺎ‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬ ‫اﯾﻦ‬ ‫اﻓﺰاﯾﻨﺪه‬ ‫ﻋﻮاﻣﻞ‬
‫ﻣﻬﻢ‬ ‫ﻧﻘﺶ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫اﺧﺘﻼل‬ ‫ﺑﺮوز‬ ‫در‬ ‫ﺗﻮاﻧﻨﺪ‬
‫ﮐﻨﻨﺪ‬ ‫اﯾﻔﺎ‬ ‫را‬ ‫اي‬ ‫ﺑﺎﻟﻘﻮه‬]12،11[‫ﻣﯿﺰان‬ ‫اﻓﺰاﯾﺶ‬ ‫ﻃﺮﻓﯽ‬ ‫از‬
‫اﯾﻦ‬ ‫در‬ ‫ﺿﺪاﺳﭙﺮم‬ ‫ﻫﺎي‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬‫ﺑﯿﻤﺎران‬‫اﻓﺰاﯾﺶ‬ ‫ﺑﺎ‬ ‫ﻫﻤﺮاه‬
‫ﮐﻪ‬ ‫رﺳﯿﺪ‬ ‫ﻧﺘﯿﺠﻪ‬ ‫اﯾﻦ‬ ‫ﺑﻪ‬ ‫ﺗﻮان‬ ‫ﻣﯽ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺳﻄﻮح‬ ‫در‬
‫ﺑﺎ‬ ‫ﻣﻮﻟﮑﻮل‬ ‫اﯾﻦ‬ ‫اﻧﺴﺪادي‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫اﺧﺘﻼل‬ ‫ﺑﺎ‬ ‫اﻓﺮاد‬ ‫در‬
‫ا‬‫روﻧﺪ‬ ‫در‬ ‫اﺧﺘﻼل‬ ‫ﺳﺒﺐ‬ ‫ﺿﺪاﺳﭙﺮم‬ ‫ﺑﺎدي‬ ‫آﻧﺘﯽ‬ ‫ﺗﻮﻟﯿﺪ‬ ‫ﻓﺰاﯾﺶ‬
‫ﮔﺮدﻧﺪ‬ ‫ﻣﯽ‬ ‫ﺗﻮﻟﯿﺪﻣﺜﻠﯽ‬]16[
‫ﭘﺮوﺗﺌﯿﻦ‬eNOS‫ﻫﺎي‬ ‫آﻣﯿﺰي‬ ‫رﻧﮓ‬ ‫ﻫﺎي‬ ‫روش‬ ‫وﺳﯿﻠﻪ‬ ‫ﺑﻪ‬
‫و‬ ‫اﺳﺖ‬ ‫ﺷﺪه‬ ‫داده‬ ‫ﻧﺸﺎن‬ ‫اﯾﻤﻮﻧﻮﻟﻮژﯾﮑﯽ‬‫ﮐﻪ‬ ‫اﺳﺖ‬ ‫ﺷﺪه‬ ‫دﯾﺪه‬
‫ﺗﺨﺮﯾﺐ‬ ‫ﺣﺎل‬ ‫در‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬‫رت‬ ‫ﻫﺎي‬ ‫ﺑﯿﻀﻪ‬ ‫در‬
‫و‬ ‫ﻧﺮﻣﺎل‬ ‫ﻫﺎي‬‫ﺻ‬ ‫ﭘﺮوﺗﺌﯿﻦ‬ ‫اﯾﻦ‬ ‫ﺑﯿﺎن‬ ‫ﮐﺮﯾﭙﺘﻮرﮐﯿﺪﯾﺴﻢ‬‫ﻣﯽ‬ ‫ﻮرت‬
‫ﭘﺬﯾﺮد‬]15،10[.‫در‬ ‫اﺳﺖ‬ ‫ﻣﻤﮑﻦ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫اﯾﻦ‬ ‫ﺑﻨﺎﺑﺮ‬
1 -Anti sperm Antibody
‫ﺗﺼﻮﯾﺮ‬2:‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬ ‫آﻣﯿﺰي‬ ‫رﻧﮓ‬eNOS‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫در‬.‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬
)‫ﭘﯿﮑﺎن‬(‫ﺧﻮﻧﯽ‬ ‫ﻋﺮوق‬ ‫دﯾﻮاره‬ ،(*)‫ﺑﺰرﮔﻨﻤﺎﯾﯽ‬x40

‫و‬ ‫ﻧﺮﻣﺎل‬ ‫ﺷﺮاﯾﻂ‬ ‫در‬ ‫ﭼﻪ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫ﻋﻤﻠﮑﺮد‬ ‫ﮐﻨﺘﺮل‬‫در‬ ‫ﭼﻪ‬
‫ﺑﺎﺷﺪ‬ ‫داﺷﺘﻪ‬ ‫ﻧﻘﺶ‬ ‫ﭘﺎﺗﻮﻟﻮژﯾﮏ‬ ‫ﺷﺮاﯾﻂ‬‫ﺗﻮﺟﻪ‬ ‫زﯾﺎدي‬ ‫ﮔﺰارﺷﺎت‬
‫درﻫ‬ ‫ﺑﻪ‬‫وزاﯾﺎ‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺑﯿﻦ‬ ‫ﮐﻨﺶ‬ ‫ﻢ‬‫دارﻧﺪ‬]
21،18،16[.‫در‬ ‫ﻃﺮﯾﻖ‬ ‫ﻫﻤﯿﻦ‬ ‫ﺑﻪ‬‫ﻫﻤﮑﻨﺶ‬1
‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺑﯿﻦ‬
‫زاﯾﺎ‬‫و‬‫ﺑﯿﺎن‬ ‫اﺳﺖ‬ ‫ﻣﻤﮑﻦ‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬eNOS‫ﻧﯿﺰ‬ ‫را‬
‫ﺳﺎزد‬ ‫ﻣﺘﺄﺛﺮ‬.‫ﺗﻮاﻧ‬ ‫ﻣﯽ‬ ‫ﺑﻨﺎﺑﺮاﯾﻦ‬،‫ﮐﻨﯿﻢ‬ ‫اﺳﺘﻨﺒﺎط‬ ‫ﻃﻮر‬ ‫اﯾﻦ‬ ‫ﯿﻢ‬
‫ﺳﻠﻮل‬ ‫اﻧﻮاع‬ ‫ﺷﺎﻫﺪ‬ ‫ﻧﯿﻔﺮ‬ ‫ﺳﻤﯽ‬ ‫ﻫﺎي‬ ‫ﺗﻮﺑﻮل‬ ‫در‬ ‫ﮐﻪ‬ ‫ﻫﻨﮕﺎﻣﯽ‬
‫ﻫﺴﺘﯿﻢ‬ ‫زاﯾﺎ‬ ‫ﻫﺎي‬،‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺸﺘﺮ‬ ‫ﺑﯿﺎن‬ ‫ﺷﺎﻫﺪ‬eNOS‫ﻣﯽ‬
‫ﺑﺎﺷﯿﻢ‬.‫در‬ ‫اﺳﺖ‬ ‫ﻣﻨﻄﻘﯽ‬‫ﻟﺤﺎظ‬ ‫از‬ ‫ﺑﯿﻤﺎران‬ ‫ﮐﻪ‬ ‫ﻣﻮاردي‬
‫ﻫﯿﺴ‬‫ﻫﺴﺘ‬ ‫ﻧﺮﻣﺎل‬ ‫ﺘﻮﻟﻮژي‬‫آ‬ ‫ﺑﯿﻤﺎران‬ ‫ﻣﺜﻞ‬ ‫ﻨﺪ‬‫زواﺳﭙﺮﻣﯽ‬
‫ﺣﺎﻟﺖ‬ ‫ﺑﻪ‬ ‫ﻧﺴﺒﺖ‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫اﻓﺮاد‬ ‫و‬ ‫اﻧﺴﺪادي‬
‫ﮐﻪ‬ ‫اﻧﺴﺪادي‬ ‫ﻏﯿﺮ‬ ‫آزواﺳﭙﺮﻣﯽ‬ ‫ﺑﯿﻤﺎران‬ ‫در‬ ‫ﻫﯿﺴﺘﻮﭘﺎﺗﻮﻟﻮژي‬
‫دارﻧﺪ‬ ‫را‬ ‫ﻫﺎ‬ ‫ﺗﻮﺑﻮل‬ ‫در‬ ‫زاﯾﺎ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﮐﺎﻫﺶ‬ ‫از‬ ‫ﻧﻤﺎﻫﺎﯾﯽ‬،
‫ﻣﯽ‬ ‫ﺑﯿﺸﺘﺮ‬ ‫اﻧﺪازه‬ ‫ﻫﻤﺎن‬ ‫ﺑﻪ‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺑﯿﺎن‬‫ﺷﻮد‬]
16[.‫ﺳﻠﻮ‬ ‫در‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﻋﻤﻠﮑﺮد‬ ‫اﻟﺒﺘﻪ‬‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫ل‬
‫اﺳﺖ‬ ‫ﻧﺸﺪه‬ ‫ﻣﺸﺨﺺ‬ ‫ﻫﻨﻮز‬]10[.‫و‬ ‫ﻓﺮﺿﯿﺎت‬ ‫ﺑﻪ‬ ‫ﺗﻮﺟﻪ‬ ‫ﺑﺎ‬ ‫اﻣﺎ‬
‫و‬ ‫ﻧﻘﺶ‬ ‫ﻓﻮق‬ ‫ﻣﻄﺎﻟﺐ‬‫ﺗﻨﻈﯿﻢ‬ ‫ﺗﻮاﻧﺪ‬ ‫ﻣﯽ‬ ‫آن‬ ‫اﺣﺘﻤﺎﻟﯽ‬ ‫ﻋﻤﻠﮑﺮد‬
‫ﺑﺎﺷﺪ‬ ‫زاﯾﺎ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺗﮑﺎﻣﻞ‬.‫ﮐﻪ‬ ‫اﺳﺖ‬ ‫ﭘﯿﺸﻨﻬﺎدﺷﺪه‬ ‫اﺧﯿﺮا‬
‫وﺟﻮد‬eNOS‫در‬ ‫ﺑﺎ‬ ‫ﺳﺮﺗﻮﻟﯽ‬ ‫ﻫﺎي‬ ‫درﺳﻠﻮل‬‫ﺑﯿﻦ‬ ‫ﻫﻤﮑﻨﺶ‬
‫زاﯾﺎ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬]23[‫و‬‫ﭘﭙﺘﯿ‬ ‫ﺗﺮاﻧﺲ‬ ‫ﮔﻠﻮﺗﺎﻣﯿﻞ‬ ‫ﮔﺎﻣﺎ‬ ‫آﻧﺰﯾﻢ‬‫ﺪاز‬
]18[‫دارد‬ ‫ارﺗﺒﺎط‬.‫در‬ ‫را‬ ‫آﻣﯿﻨﻪ‬ ‫اﺳﯿﺪﻫﺎي‬ ‫اﻧﺘﻘﺎل‬ ‫آﻧﺰﯾﻢ‬ ‫اﯾﻦ‬
‫و‬ ‫ﮐﺮده‬ ‫ﻓﻌﺎل‬ ‫ﭘﻼﺳﻤﺎﺋﯽ‬ ‫ﻏﺸﺎء‬‫ﻣﯽ‬ ‫ﺗﻨﻈﯿﻢ‬ ‫را‬ ‫ﮔﻠﻮﺗﺎﺗﯿﻮن‬ ‫ﺳﻄﺢ‬
‫ﮐﻨﺪ‬]24[.‫ﻃﺮﻓﯽ‬ ‫از‬NOS‫و‬ ‫ﺣﯿﺎﺗﯽ‬ ‫ﮐﻨﻨﺪه‬ ‫ﺗﻨﻈﯿﻢ‬ ‫ﯾﮏ‬
‫ﻣﺘﻌﺪد‬ ‫ﻫﺎي‬ ‫ﺗﻠﯿﻮم‬ ‫اﭘﯽ‬ ‫ﻧﻔﻮذﭘﺬﯾﺮي‬ ‫در‬ ‫ﻣﻬﻢ‬]23[‫ﺑﻨﺎﺑﺮاﯾﻦ‬
‫ﻫﻢ‬ ‫ﻧﯿﺰ‬ ‫ﻧﯿﻔﺮ‬ ‫ﺳﻤﯽ‬ ‫ﺗﻮﺑﻮل‬ ‫ﺗﻠﯿﻮم‬ ‫اﭘﯽ‬ ‫در‬ ‫ﻗﻮي‬ ‫اﺣﺘﻤﺎل‬ ‫ﺑﻪ‬‫ﻣﯽ‬
‫ﮐﻨﺪ‬ ‫ﻧﻘﺶ‬ ‫اﯾﻔﺎي‬ ‫ﮐﻨﻨﺪه‬ ‫ﺗﻨﻈﯿﻢ‬ ‫ﯾﮏ‬ ‫ﻋﻨﻮان‬ ‫ﺑﻪ‬ ‫ﺗﻮاﻧﺪ‬.‫ﻫﻤﺮاه‬
‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﮐﻨﻨﺪه‬ ‫ﺳﻨﺘﺰ‬ ‫آﻧﺰﯾﻢ‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬ ‫اﯾﺰوﻓﺮم‬ ‫ﺑﻮدن‬
،‫اﮐﻠﻮدﯾﻦ‬ ‫ﻗﺒﯿﻞ‬ ‫از‬ ‫ﻫﺎﺋﯽ‬ ‫ﭘﺮوﺗﺌﯿﻦ‬ ‫ﺑﺎ‬،‫اﮐﺘﯿﻦ‬‫ﺗﻮﺑﻮﻟﯿﻦ‬ ‫آﻟﻔﺎ‬1
‫ﺗﻮاﻧ‬ ‫ﻣﯽ‬‫درﮐﻨﺘﺮل‬ ‫ﮐﻪ‬ ‫ﺑﺎﺷﺪ‬ ‫ﻣﻄﻠﺐ‬ ‫اﯾﻦ‬ ‫ﻣﺆﯾﺪ‬ ‫ﺪ‬‫اﺗﺼ‬‫ﺎﻻت‬
‫اﺳﺖ‬ ‫ﻣﺆﺛﺮ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫در‬ ‫ﻣﺤﮑﻢ‬]23[.‫ﻧﺸﺎن‬ ‫ﻣﻄﺎﻟﻌﺎت‬‫داده‬
‫ﺑﯿﺎن‬ ‫ﮐﻪ‬eNOS‫و‬ ‫ﺗﺨﺮﯾﺐ‬ ‫ﺣﺎل‬ ‫در‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫در‬
‫ﺑﯿﺎن‬ ‫اﻣﺎ‬ ‫اﺳﺖ‬ ‫ﺑﻮده‬ ‫ﻫﻤﺮاه‬ ‫آﭘﻮﭘﺘﻮﺗﯿﮏ‬iNOS‫ﺑﻘﺎي‬ ‫ﺑﺎ‬ ‫ﻫﻤﺮاه‬
‫ﺗﻮﺑ‬ ‫ﺗﻠﯿﻮم‬ ‫اﭘﯽ‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫ﻧﯿﻔﺮوس‬ ‫ﺳﻤﯽ‬ ‫ﻫﺎي‬ ‫ﻮل‬
‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫ﻫﻤﺮاه‬]24[
‫ﻧﯿﺘﺮﯾﮏ‬ ‫اﻧﺪوﺗﻠﯿﺎﻟﯽ‬ ‫آﻧﺰﯾﻢ‬ ‫ﮐﺎﺗﺎﻟﯿﺰ‬ ‫از‬ ‫ﺣﺎﺻﻞ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬
‫ﭘﺎﺗﻮ‬ ‫ﺳﻄﻮح‬ ‫در‬ ‫اﮐﺴﺎﯾﺪ‬‫ﻟﻮژﯾﮏ‬‫از‬ ‫ﺑﺎﻻﺗﺮ‬)10-4
(‫ﻣﻮﻻر‬‫ﺗﺨﺮﯾﺐ‬
1 -interaction
‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬‫از‬ ‫را‬ ‫ﻧﯿﻔﺮ‬ ‫ﺳﻤﯽ‬ ‫ﻫﺎي‬ ‫ﺗﻮﺑﻮل‬ ‫ﺗﻠﯿﻮم‬ ‫اﭘﯽ‬
‫ﮐﻨﺪ‬ ‫ﻣﯽ‬ ‫اﻟﻘﺎ‬ ‫آﭘﻮﭘﺘﻮز‬ ‫ﻃﺮﯾﻖ‬.‫ﺑﯿﻦ‬ ‫از‬ ‫ﺑﺮاي‬ ‫آﭘﻮﭘﺘﻮزﯾﺲ‬ ‫ﻫﻤﻮاره‬
‫اﻓﺰاﯾﺶ‬ ‫و‬ ‫ﻣﯿﮑﻨﺪ‬ ‫اﯾﻔﺎ‬ ‫ﻧﻘﺶ‬ ‫ﻧﺎﺳﺎﻟﻢ‬ ‫ﻫﺎي‬ ‫اﺳﭙﺮم‬ ‫ﺣﺬف‬ ‫و‬ ‫ﺑﺮدن‬
‫آﻧﺰﯾﻢ‬ ‫ﺑﯿﺎن‬eNOS‫ﻏﺸﺎئ‬ ‫ﺗﺨﺮﯾﺐ‬ ‫ﺳﺒﺐ‬ ‫ﺗﻮاﻧﺪ‬ ‫ﻣﯽ‬
‫در‬ ‫ﻣﯿﺘﻮﮐﻨﺪري‬‫و‬ ‫اﺳﭙﺮم‬‫آز‬ ‫ﻧﻬﺎﯾﺘﺎ‬‫ﺳﯿﺘﻮﮐﺮوم‬ ‫ﺳﺎزي‬ ‫اد‬C‫و‬
‫آﺑﺸﺎر‬ ‫اﻓﺘﺎدن‬ ‫راه‬ ‫ﺑﻪ‬Caspase‫ﻓﺮاﯾﻨﺪ‬ ‫ﺑﯿﺸﺘﺮ‬ ‫ﺗﺤﺮﯾﮏ‬ ‫ﺑﺮاي‬
‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫آﭘﻮﭘﺘﻮزﯾﺲ‬]26،25.[
‫در‬‫ﺑﯿﺎن‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬‫آﻧﺰﯾﻢ‬eNOS‫در‬‫دو‬‫ﻣﻮرد‬ ‫ﮔﺮوه‬
‫ﺳﻮﻣﺎﺗﯿﮏ‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﺷﺪت‬ ‫آزﻣﺎﯾﺶ‬)‫ﻻﯾﺪﯾﮓ‬
‫وﺳﺮﺗﻮﻟﯽ‬(‫و‬‫ﺟﻨﺴﯽ‬)‫و‬ ‫واﺑﻨﺮﻣﺎل‬ ‫ﻧﺮﻣﺎل‬ ‫زاﯾﺎي‬ ‫ﺳﻠﻮل‬
‫ﻧﺎﺑﺎﻟﻎ‬ ‫اﺳﭙﺮﻣﺎﺗﯿﺪ‬(‫ﻗﺮارﮔﺮﻓﺖ‬ ‫ﻣﻮردارزﯾﺎﺑﯽ‬.‫ﺑﺎ‬ ‫ﻫﺎ‬ ‫ﺳﻠﻮل‬ ‫ﺗﻤﺎﻣﯽ‬
‫ﺑﻪ‬ ‫داﺷﺘﻨﺪ‬ ‫ﺑﺎﻻ‬ ‫ﺷﺪت‬ ‫ﺗﺎ‬ ‫ﺿﻌﯿﻒ‬ ‫ﭘﺬﯾﺮي‬ ‫رﻧﮓ‬ ‫ﺷﺪت‬ ‫درﺟﺎﺗﯽ‬
‫ﭘﺬﯾﺮي‬ ‫ﻓﺎﻗﺪرﻧﮓ‬ ‫ﺗﻘﺮﯾﺒﺎ‬ ‫ﮐﻪ‬ ‫ﻧﺮﻣﺎل‬ ‫زاﯾﺎي‬ ‫ﻫﺎي‬ ‫ﺳﻠﻮل‬ ‫ﺟﺰ‬
‫اﺳﺖ‬ ‫ﺑﻮده‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬.‫ازﺑﺮرﺳﯽ‬ ‫ﺣﺎﺻﻞ‬ ‫ﻫﺎي‬ ‫ﯾﺎﻓﺘﻪ‬
‫ﺣﺎﺻﻞ‬ ‫ﺑﺎﻧﺘﺎﯾﺞ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫ﻣﻮرد‬ ‫ﻫﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ ‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬
‫ﻫﺎي‬ ‫ﯾﺎﻓﺘﻪ‬ ‫از‬‫زﯾﻨﯽ‬2
‫و‬‫ﻫﻤﮑﺎراﻧﺶ‬]9[‫ﺳﺎل‬ ‫در‬1996‫ﮐﻪ‬
‫اﯾﻤﻮﻧﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ‬eNOS‫ﺑﺮ‬ ‫را‬‫واﭘﯽ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫روي‬
‫و‬ ‫دﯾﺪﯾﻢ‬‫دارد‬ ‫ﻣﻄﺎﺑﻘﺖ‬ ‫دادﻧﺪ‬ ‫ﻧﺠﺎم‬ ‫ا‬ ‫ﺳﺎﻟﻢ‬ ‫اﻓﺮاد‬ ‫در‬ ‫وازدﻓﺮان‬.
‫ﻧﺰدﯾ‬ ‫ﻣﻘﺎﻟﻪ‬ ‫ﺗﻨﻬﺎ‬ ‫ﺷﺎﯾﺪ‬ ‫ﻣﻘﺎﻟﻪ‬ ‫اﯾﻦ‬‫ﻣﺎ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫ﻣﻮرد‬ ‫ﻣﻮﺿﻮع‬ ‫ﺑﺎ‬ ‫ﮏ‬
‫ﺑﺎﺷﺪ‬.‫ﻣﻄﺎﻟﻌﺎت‬ ‫اﺻﻠﯽ‬ ‫ﻧﻘﺎﯾﺺ‬ ‫از‬ ‫ﯾﮑﯽ‬ ‫وﺟﻮد‬ ‫ﺑﺎاﯾﻦ‬‫زﯾﻨﯽ‬‫و‬
‫ﺑﺮر‬ ‫اﯾﻦ‬ ‫در‬ ‫ﻫﻤﮑﺎراﻧﺶ‬‫ﺑﻮ‬ ‫اﯾﻦ‬ ‫ﺳﯽ‬‫ﻫﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ ‫ﮐﻪ‬ ‫اﺳﺖ‬ ‫ده‬
‫ﻫﺎ‬ ‫آن‬ ‫ﮐﻨﺘﺮل‬‫ﻧﻘﺎﺋﺺ‬ ‫از‬ ‫درﺟﺎﺗﯽ‬ ‫ﮐﻪ‬ ‫ﺑﻮدﻧﺪ‬ ‫اﻓﺮادي‬
‫و‬ ‫راداﺷﺘﻪ‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬‫ﺗﺮوﻓﯽ‬ ‫ﻫﯿﭙﺮ‬ ‫ﮐﻪ‬ ‫اﻓﺮادي‬ ‫ﻫﻤﭽﻨﯿﻦ‬
‫ﭘﺮوﺳﺘﺎت‬ ‫ﺧﯿﻢ‬ ‫ﺧﻮش‬3
‫و‬ ‫ﺷﺪﻧﺪ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫وارد‬ ‫داﺷﺘﻨﺪ‬‫ﺑﻪ‬
‫ﻣﻌﻨﺎي‬ ‫ﺑﻪ‬ ‫ﺳﺎﻟﻢ‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﺎﻓﺖ‬ ‫اﻓﺮاد‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬ ‫در‬ ‫ﻋﺒﺎرﺗﯽ‬
‫ﻧﻤﻮ‬ ‫واﻗﻌﯽ‬‫ﻧ‬‫ﻧﺪاﺷﺘﻨﺪ‬ ‫ﺳﺎﻟﻢ‬ ‫ﺑﺎﻓﺘﯽ‬ ‫ﻪ‬.‫در‬‫ﻣﻄﺎﻟﻌﻪ‬ ‫در‬ ‫ﺣﺎﻟﯿﮑﻪ‬
‫اﻓﺮاد‬ ‫ﺣﺎﺿﺮ‬‫داراي‬ ‫ﮐﻪ‬ ‫ﺑﻮدﻧﺪ‬ ‫اﻓﺮادي‬ ‫ﺷﺎﻣﻞ‬ ‫ﮐﻨﺘﺮل‬ ‫ﮔﺮوه‬
‫ﻧ‬ ‫ﮐﺎﻣﻼ‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬‫و‬ ‫ﺑﻮده‬ ‫ﺳﺎﻟﻢ‬ ‫ﺑﯿﻀﻪ‬ ‫وﺑﺎﻓﺖ‬ ‫ﺮﻣﺎل‬‫ﺑﺮاي‬
‫و‬ ‫ﻧﺎﺑﺎروي‬ ‫ﺗﺸﺨﯿﺺ‬‫ﯾﺎ‬‫دﯾﮕﺮ‬ ‫دﻻﯾﻞ‬)‫داﺷﺘﻦ‬ ‫ﻣﺜﺎل‬ ‫ﻋﻨﻮان‬ ‫ﺑﻪ‬
‫ﻧﻤﻮﻧﻪ‬ ‫دادن‬ ‫در‬ ‫اﺳﺘﺮس‬(‫ﭘﺰﺷﮏ‬ ‫ﺻﻼﺣﺪﯾﺪ‬ ‫ﻃﺒﻖ‬
‫ﮔﺮﻓﺘﻨﺪ‬ ‫ﻗﺮار‬ ‫ﺑﯿﻀﻪ‬ ‫ﺑﯿﻮﭘﺴﯽ‬ ‫ﻋﻤﻞ‬ ‫ﺗﺤﺖ‬ ‫اوروﻟﻮژﯾﺴﺖ‬.
‫ﻧﺘ‬‫ﯿ‬‫ﮔﯿﺮي‬ ‫ﺠﻪ‬
‫آﻧﺰﯾﻢ‬ ‫ﻣﺨﺘﻠﻒ‬ ‫اﻧﻮاع‬ ‫از‬ ‫ﺷﺪه‬ ‫آزاد‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﮐﻪ‬ ‫ﻫﺮﭼﻨﺪ‬
‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﮐﻨﻨﺪه‬ ‫ﺳﻨﺘﺰ‬ ‫ﻫﺎي‬4
‫ﺳﻤﯽ‬ ‫ﺗﻠﯿﻮم‬ ‫اﭘﯽ‬ ‫در‬
2 -Zini
3 -Benign Prostate Hypertrophy(BPH)
4 -Nitric oxide synthase

‫وﺟﻮددارد‬ ‫ﻧﯿﻔﺮ‬‫آﻧﺰﯾﻢ‬ ‫اﯾﻦ‬ ‫ﻗﻮي‬ ‫اﺣﺘﻤﺎل‬ ‫ﺑﻪ‬ ‫اﻣﺎ‬eNOS‫اﺳﺖ‬
‫ﺑﺎﺷﻨﺪ‬ ‫ﻣﯽ‬ ‫اﺳﭙﺮﻣﺎﺗﻮژﻧﺰﯾﺲ‬ ‫ﻓﺮاﯾﻨﺪ‬ ‫اﺻﻠﯽ‬ ‫ﮐﻨﻨﺪه‬ ‫ﺗﻨﻈﯿﻢ‬ ‫ﮐﻪ‬.
‫ﻓﺮﺿﯿﻪ‬ ‫اﯾﻦ‬‫ﺟ‬ ‫در‬ ‫را‬ ‫ﺑﺎﻟﯿﻨﯽ‬ ‫ﮐﺎرﻫﺎي‬ ‫راه‬ ‫ﺗﻮاﻧﺪ‬ ‫ﻣﯽ‬‫ﻬﺖ‬
‫ا‬‫درﻣﺎﻧﯽ‬ ‫ﻫﺎي‬ ‫ﺳﺘﺮاﺗﮋي‬‫اﺧﺘﻼﻻت‬ ‫از‬ ‫ﺟﻠﻮﮔﯿﺮي‬ ‫ﺑﺮاي‬
‫ﺑﺎﺷﺪ‬ ‫داﺷﺘﻪ‬ ‫اﮐﺴﺎﯾﺪ‬ ‫ﻧﯿﺘﺮﯾﮏ‬ ‫ﺑﻪ‬ ‫واﺑﺴﺘﻪ‬ ‫آزواﺳﭙﺮﻣﯿﺎﯾﯽ‬.
‫ﺗﺸﮑﺮ‬‫ﻗﺪرداﻧﯽ‬ ‫و‬
‫در‬ ‫ﭘﮋوﻫﺸﯽ‬ ‫ﻫﺎي‬ ‫ﯾﺎﻓﺘﻪ‬ ‫از‬ ‫ﺑﺨﺸﯽ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬‫ﻣﺮﮐﺰ‬
‫ﺑﺎروري‬ ‫ﺗﺤﻘﯿﻘﺎت‬–‫ﺷﻬﯿﺪ‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ‫ﻧﺎﺑﺎروري‬
‫ﺑﺎﺷﺪ‬ ‫ﻣﯽ‬ ‫ﺑﻬﺸﺘﯽ‬.
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Journal of North Khorasan University of Medical Sciences Summer 2013;5(2):419
Immuno-histochemical localization of endothelial nitric oxide
synthase in testicular cells of men with obstructive azoospermia
Marefat ghaffari novin1
, Farhang Abed2
,Fatemeh Kiani3
,Khadijeh Foghi4*
1
faculty member of Infertility and Reproductive Health Research Center(IRHRC).Shahid Beheshti Univeristy of
Medical Sciences.Tehran.Iran
2
Sabohi infertility center, Mahdieh hospital. Infertility and Reproductive Health Research
Center(IRHRC).Tehran.Iran
3
Msc , Shahid Beheshti Univeristy of Medical Sciences,Tehran,Iran
4
faculty member of north Khorasan University of Medical Sciences. Medicine school,Anatomical sciences
Department, Tehran,Iran
Abstract
Background & Objectives: Obstructive azoospermy is one of the
cause of infertility in men which is classified as post testicular
disorders. According to previous studies patients with
azoospermia show deferent levels of eNOS enzyme.The aim of
this study was to assess the eNOS enzyme expression in patients
with azoospermia.
Method and Materials: In this case control study 10 patients
with azoospermia and 7 healthy men referred to infertility center
for treatment or diagnosis were studied. After filling consents the
testicular biopsies were obtained and fixed
Results: Leydig and sertoli cells expressed eNOS ,but germinal
normal cells did not expressed eNOS enzyme . Interestingly
eNOS enzyme expression in case group showed significant level
of difference in comparison with those of control(P<0.001).
Conclusion: according to the results of this study sertoli cells
and their intractions with germinal cells of seminiferous tubule
may play an important role in sperm quality and a successful
fertilization.
Keywords: Immunohistochemestry, eNOS, Obstructive
azoospermy ,Nitric oxide
*Corresponding Author:
North Khorasan University of
Medical Sciences, Medicine
school, ,Tehran,Iran
Email:alashtlake@gmail.com
Submitted:2013 Apr 22
Revised:2013 July 10
Accepted:2013 Sep 7
Original Article

nkums-journal-v5n2p411-en

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