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NEUROSERVICE-Corporate_2015
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- 3. www.neuroservice.com WHO WE ARE OUR SOLUTIONS OUR COMMITMENTS YOUR INTERLOCUTORS WHO WEARE OUR TECHNIQUES OUR FOCUS AREAS
- 4. www.neuroservice.com WHO WE ARE Corebusiness activity A Contract Research Organization (CRO) Testing the effect of ANY COMPOUNDS on Central Nervous System tissues
- 5. www.neuroservice.com WHAT WE DO Corebusiness activity IN VITRO TESTS UNDER PHYSIOLOGICAL CONDITIONS In rodent (or human) acute brain slices In rodent spinal cord slices In rodent cultured neurons
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- 7. www.neuroservice.com WHAT WE PROPOSEFOR DRUG DISCOVERY Your remote laboratory Hits Leads Advanced candidate IND LEAD SELECTION & OPTIMIZATION MECHANISM OF ACTION SAFETY & TOXICOLOGY PROFILING CLINICAL RESEARCH R&DPRÉ-CLINICAL DEVELOPMENT
- 8. www.neuroservice.com WHO WE ARE Keyfeatures Launched in Aix-en-Provence in 2006 Portfolio of 150 studies 60 worldwide clients (Pfizer, Roche, CHDI…) 35 people – 2 laboratories Capital: 520 000€ Income 2014 : 2,7 M€
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- 12. www.neuroservice.com OUR TECHNIQUES: THEIDEAL COMBINATION Patch-Clamp Electrode MEA Electrode
- 13. www.neuroservice.com OUR TECHNIQUES: ACOMPREHENSIVE COMBINATION Different structures and protocols MATERIAL STRUCTURE PROTOCOL Input/Output Properties Paired-Pulse Protocol Basal Synaptic Transmission Long-Term Potentiation Long-Term Depression Short-Term Potentiation Firing Activity Dose-Response Protocol Spontaneous Postsynaptic Current Agonist Evoked Postsynaptic Current Pharmacology of Intracellular Pathways Hippocampus Cortex Cerebellum Spinal Cord with or without root Ventral Tegmental Area Striatum Substantia Nigra Locus Coeruleus Periaqueductal Gray Matter Dorsal Raphe Amygdala Thalamus Dorsal Vagal Nucleus i In vitro rodent slices
- 14. www.neuroservice.com OUR TECHNIQUES: THEIDEAL COMBINATION In vitro evaluation of compounds under the most physiologically relevant conditions Robust and reproducible high quality data Reducing animal and compound use Exceptional macroscopic view of neuronal networks Ability to record multiple evoked-responses in parallel within a single slice Mid-throughput production capabilities Refined investigation of compounds’ effect on single proteins/receptors Large catalog of CNS structures and neuronal cell types to address effects on specific neurons, neurotransmitter systems or receptors NEURONAL NETWORK SINGLE NEURON PATCH-CLAMPMEA (Multi-Electrode Arrays))
- 15. www.neuroservice.com OUR FOCUS AREAS OUR SOLUTIONS OUR COMMITMENTS YOUR INTERLOCUTORS WHO WEARE OUR TECHNIQUES OUR FOCUS AREAS Drug Discovery Safety
- 16. www.neuroservice.com OUR FOCUS AREA LEAD SELECTION& OPTIMIZATION MECHANISM OF ACTION SAFETY & TOXICOLOGY PROFILING SOLUTIONS FOR CNS DRUG DISCOVERY SOLUTIONS FOR SAFETY: Testing the effect of any type of drug, molecule… on CNS and investigate its mechanism of action
- 17. www.neuroservice.com SOLUTIONS FOR DRUGDISCOVERY Example with MEA technique
- 18. www.neuroservice.com SOLUTIONS FOR DRUGDISCOVERY Example with MEA technique ACCURATE PHARMACOLOGY CHOLINERGIC RECEPTOR-MEDIATED INCREASE OF CA1 PYRAMIDAL NEURONS FIRING CARBACHOL & PIRENZEPINE M1 MUSCARINIC ACETYLCHOLINE RECEPTORS A B CPirenzepineCarbachol
- 19. www.neuroservice.com EXAMPLE WITH MEATECHNIQUE Tg Rodent Phenotyping ELECTROPHYSIOLOGY IN BRAIN SLICES FROM GENETICALLY ENGINEERED MICE: Mice deficit (or gain) determination Physiological characterizations Drug evaluation in a disease model FASTER 2 weeks LESS ANIMALS REQUIRED 10 WT / 10 Tg … in comparison with behavioural tests LESS COMPOUNDS REQUIRED
- 20. www.neuroservice.com SOLUTIONS FOR DRUGDISCOVERY Example with Patch-Clamp CHOLINERGIC RECEPTOR-MEDIATED INCREASE OF CA1 PYRAMIDAL NEURONS FIRING CARBACHOL & PIRENZEPINE EXPERIMENT Miniature inhibitory postsynaptic currents (mIPSCs) in lamina II of adult spinal cord mice DATA mIPSC frequency, amplitude distribution and detailed analysis of the average mIPSC waveform (including amplitude, rise time, decay time) 100 pA 100 s Strychnine 1 µM Rat, p15, lamina X TTX, 0.5 µM + ORG 24598, 10 µM, 35°C dorsal ventral II III 10x
- 21. www.neuroservice.com SOLUTIONS FOR DRUGDISCOVERY Example with Patch-Clamp PHARMACOLOGICAL CHARACTERIZATION OF SODIUM CURRENTS IN DRG TTXs and TTXr sodium current n=6 TTXs sodium current n=6 TTXr sodium current n=3 Mice DRG (12 weeks) Rat DRG (12 weeks) Inactivation Inactivation Inactivation
- 22. www.neuroservice.com Human tissuemanagement Protocol design Patch Clamp recording HUMAN BRAIN SLICE RECORDINGS A partnership customized to client needs EXPERT INTERFACE WITH CLIENT REMOTE LABORATORY Customer Relationship Management Quotation & Contractual Paperwork Contract and Project Management Raw data validation & Final report Clients
- 23. www.neuroservice.com HUMAN BRAIN SLICERECORDINGS STRUCTURE MATERIAL PROTOCOLS Neurons passive membrane properties AMPA/kainate “minis” or evoked-currents NMDA evoked-currents GABAA “minis” or evoked-currents … Small areas of cortical brain tissue Neurosurgical resections from adult patients For purely medical reasons (informed consent given by patients) Surgery approved by independent Board of Physicians All is supervised by the Ethical Committee from the University of Freiburg (in accordance with both national and international guidelines)
- 24. www.neuroservice.com SOLUTIONS FOR SAFETY Focusareas CUSTOMER WORK FLOW Advanced candidate MECHANISM OF ACTION SAFETY & TOXICOLOGY PROFILING CLINICAL RESEARCH Safety Studies !
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- 26. www.neuroservice.com OUR SOLUTIONS Neuroservice: yourremote laboratory Outsourced Assays R&D Customized Solutions Packages DRIVE OUR RESSOURCES CUSTOMIZE OUR ASSAYS CHOOSE A READY TO USE SOLUTION Highly experienced team in neurophysiology, neuropharmacology and neurotoxicology Portfolio of 150 studies • High quality data •Short turnaround time •Mid-throughput production capabilities 4 packages:
- 27. www.neuroservice.comwww.neuroservice.com OUTSOURCED R&D Highly skilledscientists Flexible Multi-Platform FTE Contract
- 28. www.neuroservice.comwww.neuroservice.com CUSTOMIZED SOLUTIONS More than150 studies already provided Fee for Service Contract
- 29. www.neuroservice.com NEUROSAFETY PACKAGE General profilingon synaptic properties ● At least one effect detected ! PROPOSAL FOR FURTHER INVESTIGATION
- 30. www.neuroservice.com PRO-CONVULSIVE PACKAGE Prediction ofconvulsive properties ● At least one effect detected TEST 1, TEST 2 or TEST 3 TEST 1 TEST 2 TEST 3 OK● ●● ! PROPOSAL FOR FURTHER INVESTIGATION
- 31. www.neuroservice.com GAMMA OSCILLATIONS PACKAGE Atranslational biomarker Neuronal rhythms are altered in schizophrenia, epilepsy, autism, ADHD, Alzheimer and Parkinson Diseases.
- 32. www.neuroservice.com DRG SODIUM PACKAGE(PAIN) Effects on DRG Na+ Channels Sodium channels are involved in pain associated with neuropathy, diabetes, osteoarthritis and surgery. (1 mM) (1 mM)
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- 34. www.neuroservice.com OUR COMMITMENTS QUALITY ROBUSTAND REPRODUCIBLE DATA SHORT TURNAROUND 100 VALIDATED SLICES OR 40 NEURONS / WEEK EXPERTISE INTERACTIVE AND EXPERT SUPPORT FLEXIBILITY STEP-BY-STEP STUDY PLANS ETHICS MINIMIZED ANIMAL USE
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- 36. www.neuroservice.com YOUR INTERLOCUTORS BRUNO BUISSON,PhD Founder General manager & Chief scientist SCIENTIFIC TEAM Based in JapanBased in USA TATSUO MIYAUCHI, PhD Scientific liaison JEFFREY SPROUSE, PhD Scientific liaison OLIVIER TOURY Co-Founder Deputy general manager & Chief of business development MAI SAITO Asia Business Developer LINDA HOUAICHI USA & Europe Business Developer ESTHER STEIDL, MsSc Co-founder Head of the Multi-Electrode Array laboratory BOGDAN SAVA, PhD Head of the Patch-Clamp laboratory BUSINESS TEAM
- 37. www.neuroservice.comYour partner forhands-on CNS electrophysiology Domaine de St Hilaire 595, rue Pierre CS 30531 13 593 Aix-en-Provence Cedex 3 FRANCE Tel : +33 (0)442 991 220 contact@neuroservice.com www.neuroservice.com