An enzyme assay was prepared using a robotic liquid handling system to mix substrates, enzymes, compounds, and buffer plates, followed by enzyme reactions also on the robotic system. The resulting MALDI plates were then analyzed via mass spectrometry, and the ionization data was compared between MALDI-TOF and MALDI-QqQ systems for an AChE reaction.

1. Enzyme assay preparation

2. Substrate, Enzyme, Compounds, and matrix buffer plates preparation on liquid handing Robotics system

3. Enzyme reactions on Robotic system

4. MALDI Plates ready for analysis

5. Loading MALDI plate to MS

6. MS analysis

7. Comparison of the MALDI-TOF ionization and data output with the MALDI-QqQ system.

8. AChE reaction