This document discusses micropropagation techniques that have been developed for Nepenthes species of plants. In vitro seed germination and multiplication methods have been successfully applied to both highland and lowland Nepenthes species. Proliferation was achieved using growth media containing growth regulators and coconut milk. Over 90% of Nepenthes species investigated have been propagated using these methods with no apparent deterioration even after 10 years of culture.

1. 12/08/2021 Micropropagation of Nepenthes species.
https://www.cabdirect.org/cabdirect/abstract/19910303435 1/2
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Micropropagation of Nepenthes species.
Author(s) :
Redwood, G. N. (/cabdirect/search/?q=au%3a%22Redwood%2c+G.+N.%22)
;
Bowling, J. C. (/cabdirect/search/?q=au%3a%22Bowling%2c+J.+C.%22)
Author Affiliation :
Micropropagation Unit, Royal Botanic Gardens, Kew, Richmond TW9 3AB,
UK.
Journal article
:
Botanic Gardens Micropropagation News (/cabdirect/search/?
q=do%3a%22Botanic+Gardens+Micropropagation+News%22)
1990
Vol.1
No.2
pp.19-20
ref.6
Abstract :
In vitro seed germination and multiplication techniques for Nepenthes
species have been developed at Kew and successfully applied to both highland and
lowland species. Proliferation was achieved on media containing growth regulators and
coconut milk. Activated charcoal was required for all stages apart from germination and
final multiplication. The use of antibiotics and fungicides proved beneficial in the sowing
media (Nepenthes seeds are frequently heavily contaminated and cannot withstand
prolonged sterilization). Over 90% of the species so far investigated have been
propagated; no deterioration or vitrification of the plants has been apparent, even after
10 years of culture, possibly because the plantlets are propagated from basal offshoots.
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2. 12/08/2021 Micropropagation of Nepenthes species.
https://www.cabdirect.org/cabdirect/abstract/19910303435 2/2
Record Number :
19910303435
Language of text :
English (/cabdirect/search/?q=la%3a%22English%22)
Indexing terms for this abstract:
Organism descriptor(s) :
Nepenthes, plants
Descriptor(s) :
Activated carbon, Coconut milk, culture media, germination, in vitro culture,
micropropagation, ornamental herbaceous plants, ornamental plants, plant growth regulators,
propagation, seeds, Tissue culture, utilization
Identifier(s) :
Nepenthes carunculata, Nepenthes madagascariensis, Nepenthes pervillei,
ornamentals, plant growth substances, plant hormones, plant propagation
Broader term(s) :
Nepenthaceae, Caryophyllales, eudicots, angiosperms, Spermatophyta,
plants, eukaryotes
Plants of N. madagascariensis and N. carunculata have flowered in vitro when only 30
mm high. One particularly endangered species, N. pervillei, is being grown on for
reintroduction to the Seychelles.
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Descriptors : (13)
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