Scaling-Up Yeast Fermentator to Produce Ethanol

1. Mission Statement
Ethanol production ↑
Glucose + Yeast → Ethanol Operating costs ↓
How much ethanol produced?
1 V=1000L batch fermentor
Yeast concentration?
2 X0=0.1g/L
Oxygen required?
3 S0=20g/L
Add glucose?
ChBE 229W: Fermentation Lab 1

2. Kinetic Parameters
!"#$%#&'()"#*+,-./*345*012%*
#!"
Aerobic Anaerobic Units
'&" *+,,"
decrease
Shut off O2 -,./01+"
'%"
µg 0.010 0.004 min-1
Add glucose 234560,"
'$"
!"#$%#&'()"#*+,-./*
decrease
'#"
qS 0.170 0.045 g glucose/ g cell min
'!"
increase
&"
qP 0.013 0.016 g ethanol/g cell min
%"
$"
OUR 0.0002 mmol/Ls
#"
!"
!" (!" '!!" '(!" #!!" #(!" )!!"
012%*+21#/* kL a 4.02 min-1
ChBE 229W: Fermentation Lab 2

3. Kinetic Parameters
!"#$%#&'()"#*+,-./*345*012%*
#!"
'&" *+,,"
Aerobic Anaerobic Units
'%" -,./01+"
'$"
Shut off O2 decrease
234560,"
Add glucose µg 0.010 0.004
!"#$%#&'()"#*+,-./*
min-1
'#"
decrease
qS 0.170 0.045 g glucose/ g cell min
'!"
increase
&"
qP 0.013 0.016 g ethanol/g cell min
%"
$"
OUR 0.0002 mmol/Ls
#"
kL a 4.02 min-1
!"
!" (!" '!!" '(!" #!!" #(!" )!!"
012%*+21#/*
ChBE 229W: Fermentation Lab 2

4. Scale-Up Design
*Constraints! 0.2mmol/s
1.2kg O2
Maximum * Kinetics of Ethanol Inhibition in
Glucose
alcohol fermentation, J.H.T Luong, 1984
Ethanol 70g/L
Cell 96g/L
Vacuum
Cell Balance Ethanol Balance Glucose Balance T=35°C
pH 6
V=600L
P0=0
S0=20 P1=124g/L P2=124g/L
X0=0.1 X1=20g/L X2=96g/L
0 3 6 9 12 15
Time (hr)
ChBE 229W: Fermentation Lab 3

5. Determining YX/S,YP/S and YP/X Determining µnet
23456"7"9:%"23456"8"
(#$"
("
!"#"$%&$'()"*"$%+$&,"
!"##$%"&'()*$+,-./$$2'3$0(1"$
'#$" -."#"$%,/'/&" ("
'" Aerobic Anaerobic
23456"7"
&#$" '"
&" +,-./0123"
!"#"$%/&+()"*"$%'0&1"
-."#"$%,0,1("
YX/S 0.1276 0.2056
!"##$%"&'()*$+,-./$
%#$" +./0123" &"
%"
!#$"
%"
)*+,-./"
!"
$" )01*+,-./"
)" *" %!" %&" %(" %)" %*" &!" &&" &("
23456"8"
#"
34567"8":;%"34567"9" !"
!" '!" #!!" #'!" $!!" $'!" %!!"
,"!!# 0(1"$+1(&/$
+"!!# !"#"$%'12-*"+")%,,0"
./"#"$%,112)"
*"!!#
)"!!#
Aerobic Anaerobic
Cell Balance in
34567"8"
("!!#
'"!!# -./0123# /01232$4"9:%"5678"
&"!!# -45./0123#
("
%"!!#
$"!!#
!"#"$%&'()*"+"$%,(-&"
./"#"$%,0($&" YP/S 0.1674 0.6283 Batch Reactor '"
!"!!#
!"#"$%$&''(")"$%*+$*"
!# (# $!# $(# %!# %(# ,-"#"$%''$.&"
34567"9"
&"
/01232$4"
%"
34567"8":;%"34567"9"
,"!!# $"
+"!!# !"#"$%&$'()"*"+%,&'("
-."#"'%/'&01" #"
*"!!#
)"!!#
Aerobic Anaerobic !"
34567"8"
("!!#
!" '!" #!!" #'!" $!!" $'!" %!!"
'"!!# -./012345#
5678"
&"!!#
!"#"0%,2'/)"*"'%0/&+"
-012345#
YP/X 1.2908 3.6305
%"!!# -."#"'%2$(1&"
$"!!#
!"!!#
µg=0.0199min-1
!# !"(# $# $"(# %# %"(# &# &"(#
34567"9"
ChBE 229W: Fermentation Lab ChBE 229W: Fermentation Lab 4

6. Determining qP Determining qS
Ethanol Balance in Batch Reactor Glucose Balance in Batch Reactor
ChBE 229W: Fermentation Lab ChBE 229W: Fermentation Lab 5

7. Determining OUR and kLa
!"#/.0#+,%-# Calculating OUR
!#&$"
!#&"
Calculating kLa
3rd Order Polyfit
!#%$"
OUR=0.0002mmol/Ls
!"#$%%&'()*#
!#%"
slope=-0.0002
!#!$"
is not
constant
!"
!" %!!" &!!" '!!" (!!" $!!" )!!" *!!"
+,%-#$.*#
kLa =4.02mmol/Ls
ChBE 229W: Fermentation Lab ChBE 229W: Fermentation Lab 6