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MORFOLOGI
SPERMATOZOA
SAPI BALI (BOS
SONDAICUS)
DENGAN
PEWARNAAN
WILLIAMS
1. TRY STAR GABRIELL
2. ADINDA AMALIA
3. DEWI PURNAMA SARI
4. SRI SULASTRI
5. NUR ISTIQOMAH
KELOMPOK I
(ARIFIANTINI, WRESDIYATI, RETNANI)
APA ITU
SPERMATOZOA?
Spermatozoa
Berasal dari kata sperma yang
berarti benih dan zoon yang
artinya hidup, adalah sel yang
diproduksi dari alat reproduksi
laki-laki.
Pengertian
Karakteristik
peri-akrosom
post-akrosom
Penelitian ini bertujuan untuk
mengetahui karakteristik
morfologi spermatozoa sapi Bali
menggunakan pewarnaan
Williams
Tujuan
1. Mikroskop cahaya 1000x
2. Kaca preparat dan kaca penutup
3. Pipet tetes
4. Box preparat
5. Bunsen
Alat
Bahan
1. Semen Sapi Bali
2. Alkohol 95%
3. Chloramin 0.5%
4. Alkohol Absolute
5. NaCl
6. Air
7. Minyak emersi
8. Larutan Williams
1. Disterilkan kaca preparat menggunakan bunsen
2. Semen dilarutkan dengan NaCl satu banding empat lalu dibuat
preparat ulas tipis pada gelas objek
3. Dicuci menggunakan alkohol Absolute 4 menit, lalu dikering
udarakan
4. Dimasukkan kedalam larutan 0,5% chloramin 1-2 menit
5. Dicuci pada air terdestilasi kemudian dicuci pada alkohol 95%
6. Diwarnai pada larutan WILLIAMS lalu dicuci menggunakan air
Cara Kerja
Bentuk kepala pearshaped atau
pyriform menyerupai buah pear,
dimana daerah peri-akrosom
tampak membesar dan post-
acrosom sedikit memanjang
Bentuk Pearshaped
Bentuk Narrow
Merupakan jenis kelainan kepala
dimana daerah peri-akrosom dan
post-acrosom meneyempit.
Bentuk pearshaped
Hasil
Pengamatan
peri-akrosom
post-akrosom
TERIMA KASIH
Ada pertanyaan?

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Kelompok 1 Mikroteknik.pptx

  • 1. MORFOLOGI SPERMATOZOA SAPI BALI (BOS SONDAICUS) DENGAN PEWARNAAN WILLIAMS 1. TRY STAR GABRIELL 2. ADINDA AMALIA 3. DEWI PURNAMA SARI 4. SRI SULASTRI 5. NUR ISTIQOMAH KELOMPOK I (ARIFIANTINI, WRESDIYATI, RETNANI)
  • 3. Spermatozoa Berasal dari kata sperma yang berarti benih dan zoon yang artinya hidup, adalah sel yang diproduksi dari alat reproduksi laki-laki. Pengertian Karakteristik peri-akrosom post-akrosom
  • 4. Penelitian ini bertujuan untuk mengetahui karakteristik morfologi spermatozoa sapi Bali menggunakan pewarnaan Williams Tujuan
  • 5. 1. Mikroskop cahaya 1000x 2. Kaca preparat dan kaca penutup 3. Pipet tetes 4. Box preparat 5. Bunsen Alat
  • 6. Bahan 1. Semen Sapi Bali 2. Alkohol 95% 3. Chloramin 0.5% 4. Alkohol Absolute 5. NaCl 6. Air 7. Minyak emersi 8. Larutan Williams
  • 7. 1. Disterilkan kaca preparat menggunakan bunsen 2. Semen dilarutkan dengan NaCl satu banding empat lalu dibuat preparat ulas tipis pada gelas objek 3. Dicuci menggunakan alkohol Absolute 4 menit, lalu dikering udarakan 4. Dimasukkan kedalam larutan 0,5% chloramin 1-2 menit 5. Dicuci pada air terdestilasi kemudian dicuci pada alkohol 95% 6. Diwarnai pada larutan WILLIAMS lalu dicuci menggunakan air Cara Kerja
  • 8. Bentuk kepala pearshaped atau pyriform menyerupai buah pear, dimana daerah peri-akrosom tampak membesar dan post- acrosom sedikit memanjang Bentuk Pearshaped Bentuk Narrow Merupakan jenis kelainan kepala dimana daerah peri-akrosom dan post-acrosom meneyempit. Bentuk pearshaped Hasil Pengamatan peri-akrosom post-akrosom