SlideShare a Scribd company logo
1 of 17
Download to read offline
Michael Klas
UNSW
Joint Academic Microbiology Seminars
Supervisors: Naomi Tsafnat, Mike Manefield
28 January 2015
• Mission and Motivation
o Asteroid Capture
o Envelopment
o Refuelling
• Project Objectives
o Feasibility Study
o Experimentation
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
http://www.escolar.com/avanzado/geografia004.htm
• P. aeruginosa
• E. coli
• B. subtilis
• M. barkeri
• M. mazei
• M. taylorii
Archaea Bacteria
Eukaryotes
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
http://phylogenomics.blogspot.com.au/2008/10/twisted-tree-of-life-award-2-science.html
• Substrates
o Nutrient availability
o Salinity
o pH control
• Physical Structure
• Water accessibility
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
http://deepspaceindustries.com/prospecting/
• Heat
• Gravity
• Radiation
• Vacuum
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
https://microbewiki.kenyon.edu/index.php/B
acterial_response_to_microgravity
http://www.esa.int/var/esa/storage/images/esa_multimedia/images/2012/07/space_radiati
on_affects_satellites/11258259-2-eng-GB/Space_radiation_affects_satellites.jpg
• Methane Propulsion
• Ethical & Legal Concerns
• Economy & Commerce
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
http://ingienous.com/the-challenge/green-technology/waste/space-waste/
• Asteroid Capture
o Trajectory Optimisation
• Bagging & ECLSS
o Microbial Survival
o Methane Capture
o Storage
o Refining Process
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
http://www.planetaryresources.com
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
Lab SetupPhase Diagram
En.wikipedia.org/wiki/Phase_ Diagram
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
P. aeruginosa E. coli B. subtilis
Controls
(Atmospheric Pressure)
60 min Exposure
(Low Pressure)
v
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
0
200
400
600
800
1000
1200
1400
1600
0 10 20 30 40 50 60 70
Methaneconcentration,μmol
Time, min
Methanogenesis Rate for each Timepoint
(after 6 weeks)
M. Barkeri
M. Mazei
M. Taylorii
• Potentially Feasible, more research needed
• Future Work
o More Strains
o Longer Exposure, Stronger Vacuums, Repetition
o Combined Effects Analysis
o Ecological Theory Testing
• Increased In-Situ Resource Utilisation Capabilities
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
Acknowledgments
• Dr Naomi Tsafnat
• Professor Mike Manefield
• Dr Stephen Parker
• Dr Robert Barnes
• Dr Sabrina Beckmann
• Professor Andrew Dempster
• Joel Dennerley
• Dr Gordon Roesler
• Dr Barnaby Osborne
• Professor Steven Freeland
• Mark Sonter
• http://www.planetaryresources.com/
• http://www.escolar.com/avanzado/geografia004.htm
• http://www.cmar.csiro.au/datacentre/taxonomy/mainlist.htm
• http://bucurestifm.ro/2014/09/05/un-asteroid-de-peste-30-de-metri-va-
trece-duminica-pe-langa-terra/
• http://www.xcor.com/blog/videomike/
• http://www.universetoday.com/102234/put-the-aurora-borealis-in-your-ear/
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
chaoite C
diamond C
graphite C
lonsdaleite C
cohenite (Fe,Ni)3C
haxonite (Fe,Ni)23C6
iron carbide Fe2.5C
molybdenum carbide MoC
silicon carbide SiC
titanium carbide TiC
zirconium carbide ZrC
Phosphate carbonate fluorapatiteCa5(PO4,CO3)3F
oxalate whewellite CaC2O4.H2O
various Presolar grains
Native Element
Carbide
ankerite Ca(Fe+2
,Mg,Mn)(C03)2
aragonite CaCO3
barringtonite MgCO3.2H2O
calcite CaCO3
dolomite CaMg(C03)2
hydromagnesite Mg5(C03)4(OH)2.4H20
kutnohorite Ca(Mn,Mg,Fe+2
)(C03)2
magnesite (Mg,Fe)CO3
nesquehonite Mg(HCO3)(OH).2H2O
nyerereite Na2Ca(CO3)2
reevesite Ni6Fe2(CO3)(0H)14.4H2O
rhodochrosite MnCO3
siderite FeCO3
vaterite CaCO3
zaratite Ni3(CO3)(OH)4.4H2O
Carbonate
Carbonaceous Substrates
Methanogen Database
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0 10 20 30 40 50 60
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0 10 20 30 40 50 60
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0 10 20 30 40 50 60
BacterialViability,NormalisedCFU/mL
Time, min Time, min Time, min
P. aeruginosa E. coli B. subtilis
Atmospheric Pressure Low Pressure Exposure
I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
M. barkeri M. mazei M. tayloriiLive Cells
Dead Cells
Controls
(Atmospheric Pressure)
60 min Exposure
(Low Pressure)

More Related Content

Similar to JAMS_Presentation_Michael_Klas_V2 (11)

Data integration and building a profile for yourself as an online scientist
Data integration and building a profile for yourself as an online scientistData integration and building a profile for yourself as an online scientist
Data integration and building a profile for yourself as an online scientist
 
Bionanotechnology #SciChallenge2017
Bionanotechnology #SciChallenge2017Bionanotechnology #SciChallenge2017
Bionanotechnology #SciChallenge2017
 
Microarray By Pushpita Saha
Microarray By Pushpita SahaMicroarray By Pushpita Saha
Microarray By Pushpita Saha
 
Biotische relaties ref
Biotische  relaties refBiotische  relaties ref
Biotische relaties ref
 
Data Mining Dissertations and Adventures and Experiences in the World of Chem...
Data Mining Dissertations and Adventures and Experiences in the World of Chem...Data Mining Dissertations and Adventures and Experiences in the World of Chem...
Data Mining Dissertations and Adventures and Experiences in the World of Chem...
 
How One Monkey on a Typewriter Made a Difference to Online Chemistry
How One Monkey on a Typewriter Made a Difference to Online ChemistryHow One Monkey on a Typewriter Made a Difference to Online Chemistry
How One Monkey on a Typewriter Made a Difference to Online Chemistry
 
Artemis sim 1b
Artemis sim 1bArtemis sim 1b
Artemis sim 1b
 
Isec2012 o hara
Isec2012 o haraIsec2012 o hara
Isec2012 o hara
 
stoke chemistry masterclass
stoke chemistry masterclassstoke chemistry masterclass
stoke chemistry masterclass
 
Cmat101cmatcareersslideshare
Cmat101cmatcareersslideshareCmat101cmatcareersslideshare
Cmat101cmatcareersslideshare
 
master-journey.pptx
master-journey.pptxmaster-journey.pptx
master-journey.pptx
 

JAMS_Presentation_Michael_Klas_V2

  • 1. Michael Klas UNSW Joint Academic Microbiology Seminars Supervisors: Naomi Tsafnat, Mike Manefield 28 January 2015
  • 2. • Mission and Motivation o Asteroid Capture o Envelopment o Refuelling • Project Objectives o Feasibility Study o Experimentation I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n http://www.escolar.com/avanzado/geografia004.htm
  • 3. • P. aeruginosa • E. coli • B. subtilis • M. barkeri • M. mazei • M. taylorii Archaea Bacteria Eukaryotes I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n http://phylogenomics.blogspot.com.au/2008/10/twisted-tree-of-life-award-2-science.html
  • 4. • Substrates o Nutrient availability o Salinity o pH control • Physical Structure • Water accessibility I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n http://deepspaceindustries.com/prospecting/
  • 5. • Heat • Gravity • Radiation • Vacuum I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n https://microbewiki.kenyon.edu/index.php/B acterial_response_to_microgravity http://www.esa.int/var/esa/storage/images/esa_multimedia/images/2012/07/space_radiati on_affects_satellites/11258259-2-eng-GB/Space_radiation_affects_satellites.jpg
  • 6. • Methane Propulsion • Ethical & Legal Concerns • Economy & Commerce I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n http://ingienous.com/the-challenge/green-technology/waste/space-waste/
  • 7. • Asteroid Capture o Trajectory Optimisation • Bagging & ECLSS o Microbial Survival o Methane Capture o Storage o Refining Process I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n http://www.planetaryresources.com
  • 8. I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n Lab SetupPhase Diagram En.wikipedia.org/wiki/Phase_ Diagram
  • 9. I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n P. aeruginosa E. coli B. subtilis Controls (Atmospheric Pressure) 60 min Exposure (Low Pressure) v
  • 10. I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n 0 200 400 600 800 1000 1200 1400 1600 0 10 20 30 40 50 60 70 Methaneconcentration,μmol Time, min Methanogenesis Rate for each Timepoint (after 6 weeks) M. Barkeri M. Mazei M. Taylorii
  • 11. • Potentially Feasible, more research needed • Future Work o More Strains o Longer Exposure, Stronger Vacuums, Repetition o Combined Effects Analysis o Ecological Theory Testing • Increased In-Situ Resource Utilisation Capabilities I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
  • 12. Acknowledgments • Dr Naomi Tsafnat • Professor Mike Manefield • Dr Stephen Parker • Dr Robert Barnes • Dr Sabrina Beckmann • Professor Andrew Dempster • Joel Dennerley • Dr Gordon Roesler • Dr Barnaby Osborne • Professor Steven Freeland • Mark Sonter
  • 13. • http://www.planetaryresources.com/ • http://www.escolar.com/avanzado/geografia004.htm • http://www.cmar.csiro.au/datacentre/taxonomy/mainlist.htm • http://bucurestifm.ro/2014/09/05/un-asteroid-de-peste-30-de-metri-va- trece-duminica-pe-langa-terra/ • http://www.xcor.com/blog/videomike/ • http://www.universetoday.com/102234/put-the-aurora-borealis-in-your-ear/ I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n
  • 14. chaoite C diamond C graphite C lonsdaleite C cohenite (Fe,Ni)3C haxonite (Fe,Ni)23C6 iron carbide Fe2.5C molybdenum carbide MoC silicon carbide SiC titanium carbide TiC zirconium carbide ZrC Phosphate carbonate fluorapatiteCa5(PO4,CO3)3F oxalate whewellite CaC2O4.H2O various Presolar grains Native Element Carbide ankerite Ca(Fe+2 ,Mg,Mn)(C03)2 aragonite CaCO3 barringtonite MgCO3.2H2O calcite CaCO3 dolomite CaMg(C03)2 hydromagnesite Mg5(C03)4(OH)2.4H20 kutnohorite Ca(Mn,Mg,Fe+2 )(C03)2 magnesite (Mg,Fe)CO3 nesquehonite Mg(HCO3)(OH).2H2O nyerereite Na2Ca(CO3)2 reevesite Ni6Fe2(CO3)(0H)14.4H2O rhodochrosite MnCO3 siderite FeCO3 vaterite CaCO3 zaratite Ni3(CO3)(OH)4.4H2O Carbonate Carbonaceous Substrates
  • 16. I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 0 10 20 30 40 50 60 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 0 10 20 30 40 50 60 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 0 10 20 30 40 50 60 BacterialViability,NormalisedCFU/mL Time, min Time, min Time, min P. aeruginosa E. coli B. subtilis Atmospheric Pressure Low Pressure Exposure
  • 17. I n t r o d u c t i o n • B a c k g r o u n d • D e s i g n C o n c e p t • E x p e r i m e n t a t i o n • C o n c l u s i o n M. barkeri M. mazei M. tayloriiLive Cells Dead Cells Controls (Atmospheric Pressure) 60 min Exposure (Low Pressure)