This document describes an invasive plant rapid assessment monitoring project along the 52-mile San Diego River ecosystem. The goals of the project are to establish a habitat assessment baseline, collect data to plan for treatment and removal of invasive plants, and monitor biodiversity and conservation of native flora. The methods developed include a field data sheet, survey protocol, statistics for analysis, and test runs to collect data on invasive plant distribution, canopy coverage, heights and diameters in order to map and plan management strategies.