1. DANIEL SMITH
ARGONNE NATIONAL LABORATORY

2. WHEN BIOMES COLLIDE
?
+ =

3. FIELD STUDY
 Monitoring the home and human microbiota
of relocating “citizen scientists”.

4. SAMPLING
 Human:  House:
 Palm of hand  Kitchen countertop
 Heel of foot  Kitchen floor
 Inside of nose  Kitchen light switch
 Bedroom floor
 Front door knob
 Bathroom door knob

5. ANALYSIS
PCR-Amplify 16S rDNA.
↓
Sequence 150bp x2 (paired end)
↓
Qiime Phylogenetic Assignment
↓
Principle Component Analysis

6. LIVE UPDATES
 Facebook.com/HomeMicrobiome
 Twitter.com/HomeMicrobiome
 “We’ll post all data
publicly as soon as it’s
available. If someone
can process and publish
it faster, let them.” –
Jack Gilbert