The document outlines the requirements and materials needed for a staining procedure in microbiology. It includes an inoculating loop, Bunsen burner, cotton, microscope, and specific reagents like crystal violet, grams iodine, alcohol, and safranine. These components are essential for performing the staining technique effectively.

7. Requirements
1
Materials
Slides
Sample
Inoculating loop
Bunsen burner
Cotton
Microscope
Reagents
• Primary Stain: Crystal violet
• Mordent: Grams Iodine
• Decoloriser: Alcohol
• Counterstain: Safranine