Lecture by Humbert Flamm in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III".
For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
THIS MAGAZINE BRINGS TOGETHER PREMIUM PRODUCT MANUFACTURERS AND LEADING BUILDERS TO CREATE BETTER, DIFFERENTIATED HOMES AND BUILDINGS THAT USE LESS ENERGY, SAVE WATER AND REDUCE OUR
IMPACT ON THE ENVIRONMENT.
In this presentation, Pragash talks about an automatic DD vending machine that uses ATM card for authentication. This will save time and effort for the bank and the customers will be saved from the long queues.
MAASTRO clinic is hét instituut voor radiotherapie dat de bestraling van kankerpatiënten in de provincie Limburg verzorgt.
We doen dat vanuit hoofdvestiging Maastricht en op locatie in Venlo. MAASTRO werkt intensief samen met zowel het academisch ziekenhuis Maastricht (azM) als de Universiteit Maastricht (UM).
Een samenwerkingsverband waarin we wetenschappelijk onderzoek verrichten, met o.a het azM, UM en GROW.
En dát is belangrijk om te kunnen blijven
innoveren in radiotherapie.
MAASTRO takes on cancer together with the patient and his or her relatives. Our fight against cancer is based around radiotherapy, scientific research and education. View also our general corporate presentation (on the left).The central aim of MAASTRO Clinic’s strategy is “individualised radiotherapy”. "Heading the charge for our patients!" or watch the short animation movie about Maastro's vision for cancer treatment.
Carbonic Anhydrase IX: regulation and role in cancerMAASTRO clinic
Lecture by Prof. Silvia Pastorekova in the course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
THIS MAGAZINE BRINGS TOGETHER PREMIUM PRODUCT MANUFACTURERS AND LEADING BUILDERS TO CREATE BETTER, DIFFERENTIATED HOMES AND BUILDINGS THAT USE LESS ENERGY, SAVE WATER AND REDUCE OUR
IMPACT ON THE ENVIRONMENT.
In this presentation, Pragash talks about an automatic DD vending machine that uses ATM card for authentication. This will save time and effort for the bank and the customers will be saved from the long queues.
MAASTRO clinic is hét instituut voor radiotherapie dat de bestraling van kankerpatiënten in de provincie Limburg verzorgt.
We doen dat vanuit hoofdvestiging Maastricht en op locatie in Venlo. MAASTRO werkt intensief samen met zowel het academisch ziekenhuis Maastricht (azM) als de Universiteit Maastricht (UM).
Een samenwerkingsverband waarin we wetenschappelijk onderzoek verrichten, met o.a het azM, UM en GROW.
En dát is belangrijk om te kunnen blijven
innoveren in radiotherapie.
MAASTRO takes on cancer together with the patient and his or her relatives. Our fight against cancer is based around radiotherapy, scientific research and education. View also our general corporate presentation (on the left).The central aim of MAASTRO Clinic’s strategy is “individualised radiotherapy”. "Heading the charge for our patients!" or watch the short animation movie about Maastro's vision for cancer treatment.
Carbonic Anhydrase IX: regulation and role in cancerMAASTRO clinic
Lecture by Prof. Silvia Pastorekova in the course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Measuring of biological parameters of the tumor microenvironment – advantages...MAASTRO clinic
Lecture by Andreas Weltin in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Lecture by Dr. Supuran in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III".
For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Lecture by Prof. Lambin in the course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Hypoxia as a target for personalized medicineMAASTRO clinic
Lecture by Prof. Hans Kaanders in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Tumour Hypoxia - detection and prognostic significanceMAASTRO clinic
Lecture by Dr. Heidi Lyng in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III".
For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Biological responses to tumor hypoxia & their potential as therapeutic targetsMAASTRO clinic
Lecture by Dr. Brad Wouters in the context of the Course: "Tumour Hypoxia: From Biology to Therapy III".
For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
Epigenetic Mechanisms Regulating the Cellular Response to HypoxiaMAASTRO clinic
Lecture by Prof. Lorenz Poellinger in the course: "Tumour Hypoxia: From Biology to Therapy III". For the complete e-Course see http://www.myhaikuclass.com/MaastroClinic/metoxia
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
Normal Labour/ Stages of Labour/ Mechanism of LabourWasim Ak
Normal labor is also termed spontaneous labor, defined as the natural physiological process through which the fetus, placenta, and membranes are expelled from the uterus through the birth canal at term (37 to 42 weeks
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
1. METOXIA Course
Global and local oxygen control in in vitro systems
Hubert Flamm, IMTEK Freiburg
06.10.2012
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
2. Learning objectives
• Monitoring – The only way to get oxygen under control
• Cell cultures – Physics behind oxygen distribution
• Oxygen production – Smart solution with potential
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
3. Monitoring – Its worth the effort
• Which oxygen tension does the cell see?
• Monitoring as control loop
input
• How and where can we detect pericellular oxygen levels?
− Optical dip-in sensor (Fluorescence)
− SCCF platform (Electrochemical)
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
4. Cell culture oxygenation
+ well defined environment
+ „clean room“ conditions
- long regulation path
- global regulation
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
5. Physics – Culture model
pO2 atmosphere
c0
h medium
ccells cells
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
6. Physics – Partial pressure
What is the partial pressure of oxygen gas above the medium?
n
ptotal = ∑ pi
i =1
ptotal = p N 2 + pCO2 + pO2 + pH 2O
John Dalton, 1766-1844
Assumption: Ideal gas
pO2 = vol % O2 * patm − 60hPa
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
7. Physics – Culture model
pO2 atmosphere
c0
h medium
ccells cells
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
8. Physics – Solubility of oxygen
How many oxygen disolves in the cell medium?
c0 = k H * pO2
c0 = k H (T , S ) * pO2 ( patm , pH 2O )
William Henry, 1774 -1836
c0
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
9. Physics – Culture model
pO2 atmosphere
c0
h medium
ccells cells
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
10. Physics - Oxygen diffusion
What happens if cells metabolise oxygen?
Cells consume oxygen with rate −j
pO2 atmosphere
c0
h medium
-j
ccells cells
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
11. Physics - Oxygen diffusion
What happens if cells metabolise oxygen?
Diffusion flux can be described by Fick´s first law
ccells − c0 jh
j = −D ccells = c0 +
∆h D Adolf Fick, 1829-1901
pO2 atmosphere
c0
h medium
-j
ccells cells
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
12. Oxygen control - Strategies
jh
ccells = k H (T , S ) * pO2 ( patm , p H 2O ) +
D
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
13. Oxygen control – Incubator box
• Feedback loop CELLS / INCUBATOR
– Oxygen sensor for pericellular measurement
– Software interface Sensor / Incubator
• Limitations
– Same global atmosphere for all cell cultures
– Long control loop (long response time)
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
14. Oxygen control – Single flask supply
Inside incubator:
– Individual gas supply in each (critical) flask for fine-tuning
– Easy to implement, but additional equipment needed
– Shorter regulation loop (shorter response time)
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
15. Oxygen control – Medium height
Medium height is influencing oxygen concentration at cells
jh
ccells = c0 +
D
Height could be altered:
– static (if something is known about oxygen consumption)
– dynamic with feedback to sensor reading
• Problems with forced convection
Increasing viscosity or seldom adjustments
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
16. Oxygen control – Local regulation
External source counter-acting the cellular oxygen consumption
pO2 atmosphere
c0
h medium
ccells cells
j prod > 0
Steady-state: ccells = c0 +
(j cells + j prod ) ∗ h
D
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
17. Oxygen control – Multichamber flask
• Multichamber construction
• Oxygen supply across thin
diaphragm
+ short diffusion path
+ rapid concentration change
- premixed gas required
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
18. Oxygen control – Active O2 production
• Electrolysis of aqueous solutions
• Dosing by diffusion into cell culture chamber
• Voltage / Current, Time [O2]
Cell chamber
Membrane
Electrolyte O2
Platinum electrodes
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
19. Oxygen control – Active O2 production
Cells • high aspect ratio
PDMS
• integration of micro-fluidics
Isolation
Glass Pt
• complex geometries
• plasma enhanced bonding
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
20. Oxygen control – Active O2 production
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
21. Oxygen control – Active O2 production
Video
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
22. Oxygen control – Active O2 production
300
290
2
250nA (0.476 mA/cm )
280 2
200nA (0.381 mA/cm )
oxygen / µM
2
100nA (0.190 mA/cm )
2
270 50nA (0.095 mA/cm )
2
20nA (0.038 mA/cm )
260
250
0 2 4 6 8 10
time / min
350
340
330
• Linear production
oxygen / µM
Y=A+B*X
320 Parameter Value Failure
------------------------------------------------------------
A 248,00183 2,15675
310 B 215,74322 6,16445
------------------------------------------------------------
• Wide operation range
R SD N P
300
-----------------------------------------------------------
0,99918 1,31363 4 8,15423E-4
------------------------------------------------------------
290
280
0,15 0,20 0,25 0,30 0,35 0,40 0,45 0,50
2
current density / mA/cm
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
23. Oxygen control – Active O2 production
cell chamber refill
370
350 • Fast response time
330
• Predefined profiles programmable
oxygen / µM
310
290
270
250
8 23 38 53 68 83 98 113 128
time / min
285
275
oxygen / µM
• Longtime stability 265
• Short pulses and long ramps 255
0 20 40 60 80 100 120
time / min
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
24. Oxygen control – Active O2 production
Simulation as design tool Measurement as simulation input
300
290
2
250nA (0.476 mA/cm )
280 2
200nA (0.381 mA/cm )
oxygen / µM
2
100nA (0.190 mA/cm )
2
270 50nA (0.095 mA/cm )
2
20nA (0.038 mA/cm )
260
250
0 2 4 6 8 10
time / min
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
25. Oxygen control – Active O2 production
Where to measure oxygen?
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
26. Oxygen control – Active O2 production
Simulation as guidance for design
• Lateral oxygen profiles possible
• Mimicking of reperfusion
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
27. Coffee break
• Coffee increases open-mindedness
– Coffee drinkers agree rather than others
• Coffein raises sexual longing – proven for female rats
• Coffee improves short-term memory
• Coffee consumption increases health damages caused by smoking
This course is funded with the support of the METOXIA project
06.10.12 under the FP7 Programme.
Editor's Notes
This course is funded with the support of the METOXIA project under the FP7 Programme.
Wasserdampfdruck aus Clausius-Clapeyron oder Antoine-Gleichung Dry air
Wasserdampfdruck aus Clausius-Clapeyron oder Antoine-Gleichung